scholarly journals Molecular Characterization of African Swine Fever Virus Isolates in Estonia in 2014–2019

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 582 ◽  
Author(s):  
Annika Vilem ◽  
Imbi Nurmoja ◽  
Tarmo Niine ◽  
Taavi Riit ◽  
Raquel Nieto ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Limited Area ◽  
First Case ◽  
Central Variable Region ◽  
Genotype Ii

After the extensive spread of the African swine fever virus (ASFV) genotype II in Eastern Europe, the first case of African swine fever (ASF) in Estonia was diagnosed in September 2014. By the end of 2019, 3971 ASFV-positive wild boars were found, and 27 domestic pig outbreaks were reported. A selection of ASFV isolates from wild boar and domestic pigs (during the period of September 2014–2019) was molecularly characterized using standardized genotyping procedures. One of the proven markers to characterize this virus is the central variable region (CVR) within the B602L gene. In summer 2015, a new ASFV genotype II CVR variant 2 (GII-CVR2) was confirmed in Estonia. The results suggest that the GII-CVR2 variant was only confirmed in wild boar from a limited area in southern Estonia in 2015 and 2016. In addition to GII-CVR2, a single nucleotide polymorphism (SNP) that resulted in amino acid change was identified within the genotype II CVR variant 1 (GII-CVR1). The GII-CVR1/SNP1 strain was isolated in Estonia in November 2016. Additional GII-CVR1/SNP1 cases were confirmed in two neighbouring counties, as well as in one outbreak farm in June 2017. Based on the available data, no GII-CVR2 and GII-CVR1/SNP1 have been reported by other affected European countries. The spread of variant strains in Estonia has been limited over time, and restricted to a relatively small area.

scholarly journals First Oral Vaccination of Eurasian Wild Boar Against African Swine Fever Virus Genotype II

2019 ◽  
Vol 6 ◽  
Author(s):  
Jose A. Barasona ◽  
Carmina Gallardo ◽  
Estefanía Cadenas-Fernández ◽  
Cristina Jurado ◽  
Belén Rivera ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Virus Genotype ◽  
Oral Vaccination ◽  
Genotype Ii

scholarly journals Amino Acid Tandem Repeats within a Late Viral Gene Define the Central Variable Region of African Swine Fever Virus

Virology ◽  
1996 ◽  
Vol 220 (1) ◽  
pp. 20-27 ◽  
Author(s):  
P.M. IRUSTA ◽  
M.V. BORCA ◽  
G.F. KUTISH ◽  
Z. LU ◽  
E. CALER ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tandem Repeats ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Viral Gene ◽  
Central Variable Region

scholarly journals Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1237
Author(s):  
Hyun-Joo Kim ◽  
Ki-Hyun Cho ◽  
Ji-Hyoung Ryu ◽  
Min-Kyung Jang ◽  
Ha-Gyeong Chae ◽  
...  
Keyword(s):  
South Korea ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Genetic Characteristics ◽  
Tandem Repeat Sequences ◽  
Central Variable Region ◽  
Pig Farm

On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

scholarly journals Genotyping of African Swine Fever Virus (ASFV) Isolates in Romania with the First Report of Genotype II in Symptomatic Pigs

2021 ◽  
Vol 8 (12) ◽  
pp. 290
Author(s):  
Andrei Ungur ◽  
Cristina Daniela Cazan ◽  
Luciana Cătălina Panait ◽  
Marian Taulescu ◽  
Oana Maria Balmoș ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
Animal Health ◽  
African Swine Fever ◽  
Fever Virus ◽  
Swine Industry ◽  
First Report ◽  
The World ◽  
First Time ◽  
Genotype Ii

The World Organisation for Animal Health has listed African swine fever as the most important deadly disease in domestic swine around the world. The virus was recently brought from South-East Africa to Georgia in 2007, and it has since expanded to Russia, Eastern Europe, China, and Southeast Asia, having a devastating impact on the global swine industry and economy. In this study, we report for the first time the molecular characterization of nine African swine fever virus (ASFV) isolates obtained from domestic pigs in Mureş County, Romania. All nine Romanian samples clustered within p72 genotype II and showed 100% identity with all compared isolates from Georgia, Armenia, Russia, Azerbaijan, Ukraine, Belarus, Lithuania, and Poland. This is the first report of ASFV genotype II in the country.

scholarly journals The Role of Interleukine-10 and Interferon-γ as Potential Markers of the Evolution of African Swine Fever Virus Infection in Wild Boar

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 757
Author(s):  
Sandra Barroso-Arévalo ◽  
Jose A. Barasona ◽  
Estefanía Cadenas-Fernández ◽  
José M. Sánchez-Vizcaíno
Keyword(s):  
Wild Boar ◽  
Vaccine Candidate ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Interferon Γ ◽  
Fever Virus ◽  
Control Group ◽  
Challenge Virus ◽  
Ifn Γ

African swine fever virus (ASFv) is one of the most challenging pathogens to affect both domestic and wild pigs. The disease has now spread to Europe and Asia, causing great damage to the pig industry. Although no commercial vaccine with which to control the disease is, as yet, available, some potential vaccine candidates have shown good results in terms of protection. However, little is known about the host immune mechanisms underlying that protection, especially in wild boar, which is the main reservoir of the disease in Europe. Here, we study the role played by two cytokines (IL-10 and IFN-γ) in wild boar orally inoculated with the attenuated vaccine candidate Lv17/WB/Rie1 and challenged with a virulent ASFv genotype II isolate. A group of naïve wild boar challenged with the latter isolate was also established as a control group. Our results showed that both cytokines play a key role in protecting the host against the challenge virus. While high levels of IL-10 in serum may trigger an immune system malfunctioning in challenged animals, the provision of stable levels of this cytokine over time may help to control the disease. This, together with high and timely induction of IFN-γ by the vaccine candidate, could help protect animals from fatal outcomes. Further studies should be conducted in order to support these preliminary results and confirm the role of these two cytokines as potential markers of the evolution of ASFV infection.

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