scholarly journals Anti - Dense Fıne Speckled (DFS) Antibody: its staınıng pattern in indirect immunofluorescence and its clinical relevance

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Samet Karahan ◽  
Şerife Emre ◽  
Hatice T. Hörmet-Öz
Keyword(s):  
Joint Pain ◽  
Staining Pattern ◽  
Statistical Significance ◽  
Laboratory Findings ◽  
Rheumatology Clinic ◽  
Laboratory Abnormality ◽  
Common Complaint ◽  
Antibody Positivity ◽  
Low Rate ◽  
Common Laboratory

Abstract Background: Dense fine speckled (DFS) pattern defined by very intense, heterogeneous speckled staining of nucleoplasms of interphase HEp-2 cells and chromosomal areas of metaphase cells. The association of Anti-DFS70 and rheumatologic signs, symptoms, and diagnosis were evaluated. Methods: One-hundred-eight anti-DFS70 positives who consecutively admitted Rheumatology clinic between January-June 2020 were analyzed. The clinical and laboratory findings of positives for anti-DFS70 antibody were compared with those with DFS pattern ANA IFA staining rates. Also, anti-DFS70 positivity rates and their correlation with the DFS staining pattern were analyzed retrospectively in 1016 CTD patients. Results: The most common complaint was joint pain seen in 77(71.3%) and the most common laboratory abnormality was RF-positivity observed in 10/108(9.3%) who had anti-DFS70 positivity. The most common ANA staining pattern was DFS (72/108;66,7%); one-third had other than DFS. No statistical significance was found for the association of any of the rheumatological complaints and laboratory findings with the DFS70 staining pattern. ANA analysis was performed in a total of 964/1016(94.88%) CTD patients and 44 (4.56%) of these positive for anti-DFS70. The correlation coefficient showed good correlations between the DFS pattern staining and anti-DFS70 antibody positivity (r=+0.773, p<0.001). Conclusions: Anti-DFS70-positives have a low rate of CTD. A low anti-DFS70 positivity rate was observed in patients with CTD. As such, it can be considered that anti-DFS70 does not predict CTD or even excludes it.

The clinical course of bartonellosis in 24 cats

2021 ◽  
Vol 77 (04) ◽  
pp. 65146-2021
Author(s):  
ŁUKASZ MAZUREK ◽  
OLIWIER TEODOROWSKI ◽  
PIOTR DĘBIAK ◽  
KLAUDIUSZ SZCZEPANIAK ◽  
ANNA WILCZYŃSKA ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Medical Records ◽  
Clinical Course ◽  
Bartonella Henselae ◽  
Clinical Signs ◽  
Course Of Disease ◽  
Laboratory Abnormality ◽  
Allergic Dermatitis ◽  
Time Of Presentation ◽  
Common Laboratory

The aim of this study was to analyse clinical cases of bartonellosis. Medical records of cats naturally infected with Bartonella henselae were retrospectively evaluated with regard to clinical signs and laboratory abnormalities at the time of presentation, therapy and course of disease. The most common clinical abnormalities in B. henselae-positive cats included in the study were flea allergic dermatitis (29.5%), fever (25%) and diarrhoea (12.5%). Thrombocytopenia was the most common laboratory abnormality (50%).The results of the study indicate that B. henselae infection must be considered in differential diagnosis in patients with thrombocytopenia and flea invasion.

The incidence and clinical manifestations of human brucellosis in a referral hospital in Southern Saudi Arabia between 2015 and 2019

2021 ◽  
Author(s):  
Mutasim E. Ibrahim ◽  
Mohammad S. Al-Shahrani
Keyword(s):  
Saudi Arabia ◽  
Joint Pain ◽  
Clinical Manifestations ◽  
Signs And Symptoms ◽  
Referral Hospital ◽  
Human Brucellosis ◽  
Local Health Authority ◽  
Local Health ◽  
Laboratory Findings ◽  
Body Ache

AbstractWe determined the incidence and clinical manifestations of human brucellosis from patients who attended a referral hospital in South of Saudi Arabia. A record-based retrospective study was conducted from January 2015 to December 2019 at King Abdulla Hospital, Bisha, Saudi Arabia. Information on patients’ demographic characteristics, detailed records of signs and symptoms, and the laboratory findings were reviewed. Of 6,586 patients included, 15.8% (n = 1,041) were infected with brucellosis. The age of infected individuals ranged from five to 95 years, with an average of 35.1 ± 21.2 years. Most infected patients were male (72.3%). Young adults (26–44 years) were the most common age group with the disease (34.1%). The annual rate of infection significantly decreased (P < 0.0001) from 33.2% in 2015 to 12.5% in 2019. An escalating number of brucellosis cases was seen in the spring and peaked during the summer. Fever (35.3%), joint pain (25.5%), generalized body ache (10.7%), and neurological symptoms (10.0%) were the most frequent clinical manifestation associated with brucellosis. Joint pain was commonly found among children (44.4%). Neurological findings were more frequent among adult patients. The study concluded that brucellosis is endemic in Southern Saudi Arabia and needs local health authority to implement preventive and educational program measures. Infected patients may present with diverse, nonspecific clinical manifestations that require intuition from clinicians to detect the disease.

scholarly journals Pattern and Frequency of Anti-nuclear Antibody Positivity in Paediatric Rheumatic Diseases

2019 ◽  
Vol 43 (1) ◽  
pp. 21-26
Author(s):  
Mohammad Imnul Islam ◽  
Kamrul Laila ◽  
Shahana A Rahman
Keyword(s):  
Rheumatic Diseases ◽  
Lupus Erythematosus ◽  
Staining Pattern ◽  
Renal Involvement ◽  
Clinical Manifestations ◽  
Speckled Pattern ◽  
Nuclear Antigens ◽  
Antibody Positivity ◽  
Homogeneous Pattern ◽  
Paediatric Rheumatic Diseases

Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens detected in the serum of patients with many rheumatic and non-rheumatic diseases.These antibodies are not only involved in the pathogenesis, but also constitute the basis for diagnosis and treatment of paediatric rheumatic diseases. The objective of the study was to identify the patterns and frequency of ANA positivity in Paediatric Rheumatic Diseases. Methodology: It was a retrospective study. Fourteen hundred and sixty eight records of paediaric rheumatology patients were analyzed. Statistical analysis were done to observe the frequency and association of different patterns of ANA in Juvenile idiopathic arthritis and systemic lupus erythematosus patients. Results: Among the 1468 patients of PRDs, frequency of JIA cases was the highest (65 %) followed by SLE, Scleroderma, juvenile dermatomyositis, and others. Among the 261 PRD patients ANA positivity was 65%. ANA positivity was 100%, 92%, 40% and 31.5% in Mixed connective tissue disease, SLE, JDM and Scleroderma patients respectively. Homogenous staining pattern was found in 59% and speckled pattern in 22.9%. There was significant association between ANA positivity and uveitis in oligoarticular JIA patients. Significant association was also found between homogeneous patterns of ANA and renal involvement in SLE patients. Conclusion: ANA positivity was highest in MCTD cases followed by SLE cases. Majority of SLE cases had homogeneous pattern of ANA.Staining patterns of ANA had significant association with the clinical manifestations in SLE and JIA cases. Bangladesh J Child Health 2019; VOL 43 (1) :21-26

scholarly journals Clinical Manifestation and Laboratory Finding of Sclerosis Systemic Patient in Dr. Hasan Sadikin General Hospital Bandung : A Descriptive Quantitative Study

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Annisa Meivira Budiman ◽  
Sumartini Dewi ◽  
Marietta Shanti Prananta
Keyword(s):  
Systemic Sclerosis ◽  
General Hospital ◽  
Clinical Manifestation ◽  
Clinical Symptoms ◽  
Laboratory Finding ◽  
Renal Involvement ◽  
Clinical Manifestations ◽  
Laboratory Findings ◽  
Rheumatology Clinic ◽  
Quantitative Design

Background Systemic sclerosis is a chronic progressive multisystem autoimmune disease in connective tissue, characterized by its heterogeneous clinical manifestation. The purpose of this study is to give information regarding clinical manifestations and laboratory findings of systemic sclerosis patients to establish diagnosis of disease. Methods This study was conducted using descriptive quantitative design in September−October 2016. Data was collected from medical records of patients visiting Rheumatology Clinic Dr. Hasan Sadikin General Hospital from 1 July 2015−30 June 2016 using total sampling method. The collected data were expected to comprise patient’s clinical manifestation and laboratory finding. Results Most of patients had cutaneous 57 (100.0%) and musculoskeletal 40 (70.2%) involvement. Some of the disease manifestations were Raynaud’s phenomenon 38 (66.7%), fingertip lesion 33 (57.9%), stiffness in skin 34 (59.6%), and arthalgia 29 (50.9%). Gastrointestinal involvements were present in 29 (50.9%) patients. Renal involvement were determined from urinalysis result showed proteinuria 10 (17.5%) and hematuria 8 (14.0%), found in 24 (42.1%) patients, while pulmonary and cardiac involvements were found in 30 (52.6%) patients, acknowledged from clinical symptoms such as dyspnea 12 (21.1%). Identification of autoantibodies was found in 12 (21.1%) patients, with 10 (17.5%) patients had reactive ANA and 3 (3.5%) had positive anti-Scl70. Conclusion Most of systemic sclerosis patients had cutaneous involvement. Renal, pulmonary, and cardiac involvement were concluded based on laboratory findings. Keywords: Systemic sclerosis, clinical manifestation, laboratory finding

scholarly journals Analysis of chikungunya outbreak of 2016 in Rajasthan: a clinico-epidemiological study

2018 ◽  
Vol 5 (1) ◽  
pp. 151
Author(s):  
Raman Sharma ◽  
Sunil Mahavar ◽  
Mayank Gupta ◽  
Sandeep Kumar ◽  
Jai Purohit ◽  
...  
Keyword(s):  
Epidemiological Study ◽  
Clinical Features ◽  
Joint Pain ◽  
Elevated Level ◽  
Clinical Manifestations ◽  
Cervical Lymphadenopathy ◽  
Febrile Illness ◽  
Rt Pcr ◽  
Laboratory Findings ◽  
Diagnostic Certainty

Background: To describe the diversity of clinical manifestations, laboratory findings and outcome of chikungunya fever in patients attending SMS Hospital, Jaipur during the epidemic of 2016 (September to November).Methods: All cases of febrile illness with polyarthralgia/polyarthritis diagnosed as chikungunya were analyzed. Diagnosis was made by ELISA based IgM serology and RT PCR assay.Results: A total of 200 cases were studied. All of them presented with fever, severe crippling joint pain & tenderness, headache, anorexia and body rash. On examination, there was periarticular edema, erythema, and tenderness in joints with post auricular and cervical lymphadenopathy. Unusual manifestations were hyper pigmentation of face and forehead and scrotal ulcers. On investigations patient had leucopenia with elevated level of SGOT, SGPT with normal bilirubin levels. Other complications observed were encephalopathy, encephalitis, myocarditis and hepatitis. There was no mortality in this group.Conclusions: Chikungunya though prevalent is under-reported. The diagnostic certainty is mandated by presence of febrile transiently crippling polyarthragias / arthritis. On analyzing a large series, unusual clinical features may emerge.

scholarly journals Anti-Ro/SS-A Antibody is Associated with Worse Pulmonary Outcome and Reduced Overall Survival in Systemic Sclerosis

2021 ◽  
Author(s):  
Katya Meridor ◽  
Iftach Sagy ◽  
Yair Molad
Keyword(s):  
Systemic Sclerosis ◽  
Pulmonary Function Tests ◽  
Univariate Analysis ◽  
Disease Outcome ◽  
Rheumatology Clinic ◽  
Long Term Study ◽  
All Cause Mortality ◽  
Antibody Positivity

ABSTRACT Objective We sought to determine the association of anti-Ro/SS-A antibody with organ involvement and disease outcome, in patients with systemic sclerosis (SSc). Methods A retrospective, long-term study of a cohort of incident patients diagnosed with SSc, and continuously followed at our rheumatology clinic during 1990-2018. Results Included were 105 patients with known anti-Ro/SS-A antibody status, 92.4% female, mean age at diagnosis 52.0±15.6 years, and median follow-up 10 years; 64% were diagnosed with limited cutaneous SSc, 18% with diffuse cutaneous SSc, and 18% had SSc siné scleroderma or undetermined disease type. Anti-Ro/SS-A antibody tested positive in 21% of patients. In univariate analysis, anti-Ro/SS-A antibody-positivity was significantly associated with SSc overlap with Sjogren’s syndrome (p &lt;0.001). Pulmonary function tests (PFT) deterioration at last encounter was significantly associated with anti-Ro/SS-A antibody-positivity. In multivariate regression for anti-Ro/SS-A antibody-positive SSc patients and disease outcome (adjusted for age&gt;50 years, smoking, and baseline predicted forced vital capacity (pFVC) &lt; 80%), positive anti-Ro/SS-A antibody was significantly associated with higher all-cause mortality rate (HR 5.17, CI 95% 1.18-22.67, p=0.029), and greater deterioration of pFVC defined as decrement of last available pFVC compared to first available pFVC of ≥10% (HR 3.65, CI 95% 1.07-12.38, p=0.038). Conclusions Anti-Ro/SS-A antibody is an independent risk factor for worse pulmonary outcome and higher all-cause mortality in patients with SSc, independent of SSc clinical and/or serological subtype.

scholarly journals Clinical and laboratory findings in patients with dengue associated with hepatopathy

2011 ◽  
Vol 44 (6) ◽  
pp. 674-677 ◽  
Author(s):  
Delso do Nascimento ◽  
Ana Rita Coimbra Motta de Castro ◽  
Íris Bucker Froes ◽  
Gláucia Bigaton ◽  
Éveny Cristine Luna de Oliveira ◽  
...  
Keyword(s):  
Low Income ◽  
Clinical Symptoms ◽  
Statistical Significance ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Laboratory Findings ◽  
Mato Grosso ◽  
Mortality And Morbidity ◽  
Average Value ◽  
Grade Ii

INTRODUCTION: Hepatic disorders caused by dengue infection may progress to severe manifestations, including mortality and morbidity. Cytokines are involved in it, such as the migration inhibitory factor of macrophages (MIF), tumor necrosis factor (TNF), natural killer cells (NK), B lymphocytes, and macrophages. METHODS: This study was carried out from January to April 2007 at a public hospital from the Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Sixty-eight patients were studied concerning hepatic alterations, with 56 reported having classic dengue, 6 with hemorrhagic dengue grade I, and 6 with hemorrhagic dengue grade II. RESULTS: Among the 56 with classic dengue, 83.3% had aspartate aminotransferase (AST) alterations, and 69.6% had altered alanine aminotransferase (ALT). For those with hemorrhagic dengue grade I, 100% had AST alterations, and 83.3% had altered ALT. All the patients with hemorrhagic dengue grade II had AST and ALT alterations. AST variations reached 22.0 and 907.0, with an average value of 164.6. For ALT, we found variations between 25.0 and 867.0, with an average value of 166.07. There had been statistical significance between dengue clinical shapes and hepatic function markers. CONCLUSIONS: We conclude that the infection was predominant in adults, females, and in those with low income and education. The liver enzymes were of larger amount in hemorrhagic dengue, but there was weak statistical evidence of the clinical manifestations and transaminases. Major signs and clinical symptoms were fever, headache, myalgia, arthralgia, weakness, severe pain behind the eyes, and rashes.

Initial Presentation of Granulomatosis with Polyangiitis as Progressive Skull Base Osteomyelitis

ORL ◽  
2021 ◽  
pp. 1-5
Author(s):  
Hannan A. Qureshi ◽  
Anshu Bandhlish ◽  
Robert P. DeConde ◽  
Ian M. Humphreys ◽  
Waleed M. Abuzeid ◽  
...  
Keyword(s):  
Skull Base ◽  
Endoscopic Sinus Surgery ◽  
Granulomatosis With Polyangiitis ◽  
Abducens Nerve ◽  
Sinus Surgery ◽  
High Dose ◽  
Laboratory Findings ◽  
Skull Base Osteomyelitis ◽  
Antibody Positivity ◽  
Fluid Collections

A healthy man in his 30s presented with a 2-week history of severe bitemporal pain and pressure. He was initially treated for presumed acute rhinosinusitis, but his symptoms continued to worsen and underwent endoscopic sinus surgery at an outside community facility. He developed left abducens nerve palsy postoperatively, and magnetic resonance imaging (MRI) demonstrated evidence of extensive skull base osteomyelitis. He was initiated on intravenous (IV) broad-spectrum antibiotics but was subsequently found to have prostatic and submandibular sterile fluid collections. The patient subsequently developed new right abducens and left vagal nerve palsies and underwent revision endoscopic sinus surgery. Pathology revealed extensive inflammation, necrotizing granulomas, and evidence of small and medium vessel vasculitis. Extensive laboratory workup was negative, except for anti-PR-3 antibody positivity. Given the characteristic findings on pathology and laboratory findings, the patient was diagnosed with granulomatosis with polyangiitis (GPA). High-dose glucocorticoid therapy as well as rituximab infusion were promptly initiated. He had marked improvement in his symptoms and resolution of his right CN VI palsy but left-sided CN VI and CN X palsies persisted. This patient presented without the typical rhinologic manifestations of GPA, and rather presented with progressive sinusitis, skull base osteomyelitis with associated cranial neuropathies, and aseptic systemic abscesses. Prompt diagnosis of GPA is particularly important in those with otorhinolaryngological manifestations, as early initial immunosuppressive therapy has been linked to lower relapse and mortality rates. Vigilance and early differentiation between GPA and other forms of sinusitis is of critical importance, particularly when symptoms are refractory to standard rhinosinusitis therapies.

Children With Partial IgA Deficiency

2011 ◽  
Vol 51 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Aarti Shakkottai ◽  
Krishnaveni Bupathi ◽  
Ankit P. Patel ◽  
Elizabeth Chalom ◽  
Suneetha Chamarthi ◽  
...  
Keyword(s):  
Joint Pain ◽  
Antinuclear Antibody ◽  
Chart Review ◽  
Morning Stiffness ◽  
Clinical Manifestations ◽  
Retrospective Chart Review ◽  
Iga Deficiency ◽  
Rheumatology Clinic ◽  
History Of ◽  
Pediatric Rheumatology Clinic

Literature is lacking on partial IgA deficiency. In this study, the authors propose to describe the clinical manifestations of patients with partial IgA deficiency. Methods. The authors conducted a retrospective chart review of 13 patients with partial IgA deficiency followed at the pediatric rheumatology clinic at Robert Wood Johnson Medical School. They looked for the presence of rashes, joint pain, joint swelling, and morning stiffness. The authors also examined charts for a history of frequent infections, allergies, and the presence of elevated antinuclear antibody. Results. Eleven out of the 13 patients complained of joint pain, joint swelling, or morning stiffness. Six patients carried a diagnosis of a definitive rheumatic disease. Four patients suffered from frequent infections and 2 patients reported allergies. Conclusion. Partial IgA deficiency appears to be associated with rheumatic diseases and complaints of joint pain, joint swelling, and morning stiffness. A larger study is needed to confirm these results.

scholarly journals Recurrent Nephritis and/or Pulmonary Hemorrhage in Patients with Anti-Glomerular Basement Membrane Disease with and without ANCA Positivity

2021 ◽  
pp. 1-8
Author(s):  
Nicole Droz ◽  
Alexis Katz ◽  
Aditi Patel ◽  
Isaac Briskin ◽  
John Sedor ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Statistical Significance ◽  
Disease Onset ◽  
Clinical Manifestations ◽  
Pulmonary Hemorrhage ◽  
High Incidence ◽  
Long Term Follow Up ◽  
Antibody Positivity

<b><i>Introduction:</i></b> Anti-glomerular basement membrane (anti-GBM) disease is characterized by rapidly progressive glomerular nephritis with or without pulmonary hemorrhage with disease severity correlating with antibody titer. Following treatment, relapse is rare but has been reported in the literature. The objective of this study was to assess for clinical, serologic, and histologic differences associated with disease relapse in patients with anti-GBM disease. <b><i>Methods:</i></b> Patients seen at our facility between 1997 and 2017 were screened for anti-GBM disease by ICD 9/10 codes. They were included if the diagnosis was confirmed by a board-certified rheumatologist or nephrologist and had positive antibodies and/or biopsy results consistent with anti-GBM disease. Relapsing disease was defined as recurrence of pulmonary or renal manifestations after achieving remission following the initial presentation. All charts were reviewed for baseline demographics, clinical manifestations, and antibody positivity and compared between groups. <b><i>Results:</i></b> 40 patients were confirmed as having anti-GBM disease. Mean follow-up from disease onset to the date of last follow-up was 56.2 months. 8 patients had relapsing disease and 32 patients had nonrelapsing disease. Baseline characteristics and clinical manifestations were similar between groups. Patients with relapsing disease had a high incidence of anti-neutrophilic cytoplasmic antibody (ANCA) co-positivity as compared to nonrelapsing patients (50 vs. 15.6%, respectively, <i>p</i> = 0.059), but this did not reach statistical significance. In patients with relapsing disease, only one had positive anti-GBM antibodies at time of relapse. <b><i>Conclusions:</i></b> In this study, patients with relapsing disease had a high incidence of ANCA co-positivity (50%). In patients with newly diagnosed anti-GBM disease, ANCAs should be obtained to assess for the risk of relapse and to help guide long-term follow-up and treatment.

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