scholarly journals Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle

Open Physics ◽  
2014 ◽  
Vol 12 (3) ◽  
Author(s):  
Maja Miletić ◽  
Dragana Vuković ◽  
Irena Živanović ◽  
Ivana Dakić ◽  
Ivan Soldatović ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Plasma Chemistry ◽  
Atmospheric Pressure Plasma ◽  
Plasma Source ◽  
Methicillin Resistant ◽  
Plasma Sources ◽  
Equilibrium Plasma ◽  
Plasma Needle

AbstractIn numerous recent papers plasma chemistry of non equilibrium plasma sources operating at atmospheric pressure has been linked to plasma medical effects including sterilization. In this paper we present a study of the effectiveness of an atmospheric pressure plasma source, known as plasma needle, in inhibition of the growth of biofilm produced by methicillin resistant Staphylococcus aureus (MRSA). Even at the lowest powers the biofilms formed by inoculi of MRSA of 104 and 105 CFU have been strongly affected by plasma and growth in biofilms was inhibited. The eradication of the already formed biofilm was not achieved and it is required to go to more effective sources.

scholarly journals Cold Atmospheric-Pressure Plasma Caused Protein Damage in Methicillin-Resistant Staphylococcus aureus Cells in Biofilms

2021 ◽  
Vol 9 (5) ◽  
pp. 1072
Author(s):  
Li Guo ◽  
Lu Yang ◽  
Yu Qi ◽  
Gulimire Niyazi ◽  
Lingling Huang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Atmospheric Pressure Plasma ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Methicillin Resistant ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Biofilms formed by multidrug-resistant bacteria are a major cause of hospital-acquired infections. Cold atmospheric-pressure plasma (CAP) is attractive for sterilization, especially to disrupt biofilms formed by multidrug-resistant bacteria. However, the underlying molecular mechanism is not clear. In this study, CAP effectively reduced the living cells in the biofilms formed by methicillin-resistant Staphylococcus aureus, and 6 min treatment with CAP reduced the S. aureus cells in biofilms by 3.5 log10. The treatment with CAP caused the polymerization of SaFtsZ and SaClpP proteins in the S. aureus cells of the biofilms. In vitro analysis demonstrated that recombinant SaFtsZ lost its self-assembly capability, and recombinant SaClpP lost its peptidase activity after 2 min of treatment with CAP. Mass spectrometry showed oxidative modifications of a cluster of peaks differing by 16 Da, 31 Da, 32 Da, 47 Da, 48 Da, 62 Da, and 78 Da, induced by reactive species of CAP. It is speculated that the oxidative damage to proteins in S. aureus cells was induced by CAP, which contributed to the reduction of biofilms. This study elucidates the biological effect of CAP on the proteins in bacterial cells of biofilms and provides a basis for the application of CAP in the disinfection of biofilms.

scholarly journals Cold Atmospheric Pressure Microplasma Pipette for Disinfection of Methicillin-Resistant Staphylococcus aureus

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1103
Author(s):  
Geunyoung Nam ◽  
Muhwan Kim ◽  
Yeonsook Jang ◽  
Sungbo Cho
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Heat Radiation ◽  
Methicillin Resistant ◽  
Solid Agar ◽  
Microbial Infections ◽  
Cold Atmospheric Pressure Plasma

Microbial infections should be controlled and prevented for successful wound healing and tissue regeneration. Various disinfection methods exist that use antibiotics, ultraviolet (UV), heat, radiation, or chemical disinfectants; however, cold atmospheric pressure plasma has exhibited a unique and effective antibacterial ability that is not affected by antibiotic resistance or pain. This study develops a cold atmospheric pressure microplasma pipette (CAPMP) that outputs an Ar plasma plume through a tube with an inner radius of 180 μm for disinfection in a small area. The CAPMP was evaluated using Staphylococcus aureus and methicillin-resistant Staphylococcus aureus diluted in liquid media, spread on solid agar, or covered by dressing gauze. An increase in the treatment time of CAPMP resulted in a decrease in the number of colonies of the grown microorganism (colony forming unit) and an increase in the disinfected area for both bacteria. The disinfection ability of CAPMP was observed when the bacteria were covered with dressing gauze and was dependent on the number of gauze layers.

scholarly journals Plasma-Activated Saline Promotes Antibiotic Treatment of Systemic Methicillin-Resistant Staphylococcus aureus Infection

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1018
Author(s):  
Lu Yang ◽  
Gulimire Niyazi ◽  
Yu Qi ◽  
Zhiqian Yao ◽  
Lingling Huang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Combined Treatment ◽  
Atmospheric Pressure Plasma ◽  
Systemic Infection ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Methicillin Resistant ◽  
New Strategy ◽  
Hematological And Biochemical Parameters

Systemic infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are life-threatening due to their strong multidrug resistance, especially since the biofilms formed by MRSA are more difficult to inactivate by antibiotics, causing long term recurrence of infection. Plasma-activated saline (PAS), a derived form of cold atmospheric-pressure plasma, can effectively inactivate bacteria and cancer cells and has been applied to sterilization and cancer treatment. Previous studies have demonstrated that the pretreatment of MRSA with PAS could promote the action of antibiotics. Here, the PAS was used as an antibiotic adjuvant to promote the inactivation of MRSA biofilms by rifampicin and vancomycin, and the combined treatment reduced approximately 6.0-log10 MRSA cells in biofilms. The plasma-activated saline and rifampicin synergistically and effectively reduced the systemic infection in the murine model. The histochemical analysis and the blood hematological and biochemical test demonstrated that the combined treatment with plasma-activated saline and rifampicin improved the blood hematological and biochemical parameters of infected mice by reducing the infection. Therefore, PAS based on plasma technology represents a new strategy for the treatment of infectious disease caused by multidrug-resistant bacteria and alleviating antibiotic resistance.

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