scholarly journals Cold Atmospheric-Pressure Plasma Caused Protein Damage in Methicillin-Resistant Staphylococcus aureus Cells in Biofilms

2021 ◽  
Vol 9 (5) ◽  
pp. 1072
Author(s):  
Li Guo ◽  
Lu Yang ◽  
Yu Qi ◽  
Gulimire Niyazi ◽  
Lingling Huang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Atmospheric Pressure Plasma ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Methicillin Resistant ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Biofilms formed by multidrug-resistant bacteria are a major cause of hospital-acquired infections. Cold atmospheric-pressure plasma (CAP) is attractive for sterilization, especially to disrupt biofilms formed by multidrug-resistant bacteria. However, the underlying molecular mechanism is not clear. In this study, CAP effectively reduced the living cells in the biofilms formed by methicillin-resistant Staphylococcus aureus, and 6 min treatment with CAP reduced the S. aureus cells in biofilms by 3.5 log10. The treatment with CAP caused the polymerization of SaFtsZ and SaClpP proteins in the S. aureus cells of the biofilms. In vitro analysis demonstrated that recombinant SaFtsZ lost its self-assembly capability, and recombinant SaClpP lost its peptidase activity after 2 min of treatment with CAP. Mass spectrometry showed oxidative modifications of a cluster of peaks differing by 16 Da, 31 Da, 32 Da, 47 Da, 48 Da, 62 Da, and 78 Da, induced by reactive species of CAP. It is speculated that the oxidative damage to proteins in S. aureus cells was induced by CAP, which contributed to the reduction of biofilms. This study elucidates the biological effect of CAP on the proteins in bacterial cells of biofilms and provides a basis for the application of CAP in the disinfection of biofilms.

scholarly journals Plasma-Activated Saline Promotes Antibiotic Treatment of Systemic Methicillin-Resistant Staphylococcus aureus Infection

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1018
Author(s):  
Lu Yang ◽  
Gulimire Niyazi ◽  
Yu Qi ◽  
Zhiqian Yao ◽  
Lingling Huang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Combined Treatment ◽  
Atmospheric Pressure Plasma ◽  
Systemic Infection ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Methicillin Resistant ◽  
New Strategy ◽  
Hematological And Biochemical Parameters

Systemic infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are life-threatening due to their strong multidrug resistance, especially since the biofilms formed by MRSA are more difficult to inactivate by antibiotics, causing long term recurrence of infection. Plasma-activated saline (PAS), a derived form of cold atmospheric-pressure plasma, can effectively inactivate bacteria and cancer cells and has been applied to sterilization and cancer treatment. Previous studies have demonstrated that the pretreatment of MRSA with PAS could promote the action of antibiotics. Here, the PAS was used as an antibiotic adjuvant to promote the inactivation of MRSA biofilms by rifampicin and vancomycin, and the combined treatment reduced approximately 6.0-log10 MRSA cells in biofilms. The plasma-activated saline and rifampicin synergistically and effectively reduced the systemic infection in the murine model. The histochemical analysis and the blood hematological and biochemical test demonstrated that the combined treatment with plasma-activated saline and rifampicin improved the blood hematological and biochemical parameters of infected mice by reducing the infection. Therefore, PAS based on plasma technology represents a new strategy for the treatment of infectious disease caused by multidrug-resistant bacteria and alleviating antibiotic resistance.

scholarly journals Synthesis of photo-excited Chlorin e6 conjugated silica nanoparticles for enhanced anti-bacterial efficiency to overcome methicillin-resistant Staphylococcus aureus

2019 ◽  
Vol 55 (18) ◽  
pp. 2656-2659 ◽  
Author(s):  
Jia-fu Lin ◽  
Juan Li ◽  
Ashna Gopal ◽  
Tasnim Munshi ◽  
Yi-wen Chu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Silica Nanoparticles ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Chlorin E6 ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Methicillin Resistant

Nano photodynamic therapy to overcome multidrug resistant bacteria.

scholarly journals Cold Atmospheric Pressure Microplasma Pipette for Disinfection of Methicillin-Resistant Staphylococcus aureus

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1103
Author(s):  
Geunyoung Nam ◽  
Muhwan Kim ◽  
Yeonsook Jang ◽  
Sungbo Cho
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Heat Radiation ◽  
Methicillin Resistant ◽  
Solid Agar ◽  
Microbial Infections ◽  
Cold Atmospheric Pressure Plasma

Microbial infections should be controlled and prevented for successful wound healing and tissue regeneration. Various disinfection methods exist that use antibiotics, ultraviolet (UV), heat, radiation, or chemical disinfectants; however, cold atmospheric pressure plasma has exhibited a unique and effective antibacterial ability that is not affected by antibiotic resistance or pain. This study develops a cold atmospheric pressure microplasma pipette (CAPMP) that outputs an Ar plasma plume through a tube with an inner radius of 180 μm for disinfection in a small area. The CAPMP was evaluated using Staphylococcus aureus and methicillin-resistant Staphylococcus aureus diluted in liquid media, spread on solid agar, or covered by dressing gauze. An increase in the treatment time of CAPMP resulted in a decrease in the number of colonies of the grown microorganism (colony forming unit) and an increase in the disinfected area for both bacteria. The disinfection ability of CAPMP was observed when the bacteria were covered with dressing gauze and was dependent on the number of gauze layers.

scholarly journals High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal

2020 ◽  
Vol 8 (10) ◽  
pp. 1487
Author(s):  
Marta Aires-de-Sousa ◽  
Claudine Fournier ◽  
Elizeth Lopes ◽  
Hermínia de Lencastre ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Species ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Encoding Genes

In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended-spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for vancomycin-resistant Enterococci (VRE). A large proportion of samples yielded carbapenemase- or ESBL-producing Enterobacteriaceae (16% and 55%, respectively), while only two MRSA and two VRE were detected. Mating-out assays followed by PCR and whole-plasmid sequencing allowed to identify carbapenemase and ESBL encoding genes. Among 24 carbapenemase-producing isolates, there were mainly Klebsiella pneumoniae (50%) and Escherichia coli (33%). OXA-181 was the most common carbapenemase identified (54%), followed by OXA-48 (25%) and KPC-2 (17%). Ten different ESBLs were found among 62 ESBL-producing isolates, mainly being CTX-M-type enzymes (87%). Co-occurrence in single samples of multiple ESBL- and carbapenemase producers belonging to different bacterial species was observed in some cases. Seagulls constitute an important source for spreading multidrug-resistant bacteria in the environment and their gut microbiota a formidable microenvironment for transfer of resistance genes within bacterial species.

scholarly journals Nitrogen Functionalities of Amino-Functionalized Nitrogen-Doped Graphene Quantum Dots for Highly Efficient Enhancement of Antimicrobial Therapy to Eliminate Methicillin-Resistant Staphylococcus aureus and Utilization as a Contrast Agent

2021 ◽  
Vol 22 (18) ◽  
pp. 9695
Author(s):  
Wen-Shuo Kuo ◽  
Ping-Ching Wu ◽  
Chi-Yao Hung ◽  
Chia-Yuan Chang ◽  
Jiu-Yao Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Quantum Dots ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Continuous Wave ◽  
Graphene Quantum Dots ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Methicillin Resistant ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

There is an urgent need for materials that can efficiently generate reactive oxygen species (ROS) and be used in photodynamic therapy (PDT) as two-photon imaging contrast probes. In this study, graphene quantum dots (GQDs) were subjected to amino group functionalization and nitrogen doping (amino-N-GQDs) via annealing and hydrothermal ammonia autoclave treatments. The synthesized dots could serve as a photosensitizer in PDT and generate more ROS than conventional GQDs under 60-s low-energy (fixed output power: 0.07 W·cm−2) excitation exerted by a 670-nm continuous-wave laser. The generated ROS were used to completely eliminate a multidrug-resistant strain of methicillin-resistant Staphylococcus aureus (MRSA), a Gram-positive bacterium. Compared with conventional GQDs, the amino-N-GQDs had superior optical properties, including stronger absorption, higher quantum yield (0.34), stronger luminescence, and high stability under exposure. The high photostability and intrinsic luminescence of amino-N-GQDs contribute to their suitability as contrast probes for use in biomedical imaging, in addition to their bacteria tracking and localization abilities. Herein, the dual-modality amino-N-GQDs in PDT easily eliminated multidrug-resistant bacteria, ultimately revealing their potential for use in future clinical applications.

scholarly journals Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle

Open Physics ◽  
2014 ◽  
Vol 12 (3) ◽  
Author(s):  
Maja Miletić ◽  
Dragana Vuković ◽  
Irena Živanović ◽  
Ivana Dakić ◽  
Ivan Soldatović ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atmospheric Pressure ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Plasma Chemistry ◽  
Atmospheric Pressure Plasma ◽  
Plasma Source ◽  
Methicillin Resistant ◽  
Plasma Sources ◽  
Equilibrium Plasma ◽  
Plasma Needle

AbstractIn numerous recent papers plasma chemistry of non equilibrium plasma sources operating at atmospheric pressure has been linked to plasma medical effects including sterilization. In this paper we present a study of the effectiveness of an atmospheric pressure plasma source, known as plasma needle, in inhibition of the growth of biofilm produced by methicillin resistant Staphylococcus aureus (MRSA). Even at the lowest powers the biofilms formed by inoculi of MRSA of 104 and 105 CFU have been strongly affected by plasma and growth in biofilms was inhibited. The eradication of the already formed biofilm was not achieved and it is required to go to more effective sources.

scholarly journals Presence of Methicillin-Resistant Staphylococcus aureus (MRSA) on Healthcare Workers’ Attire: A Systematic Review

2021 ◽  
Vol 6 (2) ◽  
pp. 42
Author(s):  
Pavlina Lena ◽  
Angela Ishak ◽  
Spyridon A Karageorgos ◽  
Constantinos Tsioutis
Keyword(s):  
Systematic Review ◽  
Staphylococcus Aureus ◽  
Healthcare Workers ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Primary Study ◽  
Resistant Bacteria ◽  
Culture Methods ◽  
Methicillin Resistant ◽  
Healthcare Facilities

Contaminated healthcare workers’ (HCW) clothing risk transferring methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. We performed a systematic review in Pubmed and Scopus for 2000–2020 according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines to analyze evidence of MRSA on HCW attire. The primary study outcome was MRSA isolation rates on HCW clothing in healthcare settings. Out of 4425 articles, 23 studies were included: 18 with 1760 HCWs, four with 9755 HCW–patient interactions and one with 512 samples. There was a notable variation in HCWs surveyed, HCW attires, sampling techniques, culture methods and laundering practices. HCW attire was frequently colonized with MRSA with the highest rates in long-sleeved white coats (up to 79%) and ties (up to 32%). Eight studies reported additional multidrug-resistant bacteria on the sampled attire. HCW attire, particularly long-sleeved white coats and ties, is frequently contaminated with MRSA. Banning certain types and giving preference to in-house laundering in combination with contact precautions can effectively decrease MRSA contamination and spread.

scholarly journals Polymeric approach to combat drug-resistant methicillin-resistant Staphylococcus aureus

2021 ◽  
Vol 56 (12) ◽  
pp. 7265-7285
Author(s):  
Shreya Kanth ◽  
Akshatha Nagaraja ◽  
Yashoda Malgar Puttaiahgowda
Keyword(s):  
Staphylococcus Aureus ◽  
Death Rate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Pathogenic Microorganisms ◽  
Polymeric Coatings ◽  
Methicillin Resistant ◽  
Drug Resistant Bacteria

Abstract The current global death rate has threatened humans due to increase in deadly unknown infections caused by pathogenic microorganisms. On the contrary, the emergence of multidrug-resistant bacteria is also increasing which is leading to elevated lethality rate worldwide. Development of drug-resistant bacteria has become one of the daunting global challenges due to failure in approaching to combat against them. Methicillin-resistant Staphylococcus aureus (MRSA) is one of those drug-resistant bacteria which has led to increase in global mortality rate causing various lethal infections. Polymer synthesis can be one of the significant approaches to combat MRSA by fabricating polymeric coatings to prevent the spread of infections. This review provides last decade information in the development of various polymers against MRSA. Graphical abstract

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