Synergy between Salvia officinalis L. and some preservatives

AbstractThe aim of the present study is to investigate the antibacterial activity of Salvia officinalis L. aqueous extracts and its synergistic action with preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergy was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/ sodium benzoate, aqueous extract/ potassium sorbate, aqueous extract/ sodium nitrite combinations. Synergy was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergy was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values.

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In vitro synergistic antibacterial activity of Salvia officinalis L. and some preservatives

Archives of Biological Sciences ◽

10.2298/abs1001167s ◽

2010 ◽

Vol 62 (1) ◽

pp. 167-174 ◽

Cited By ~ 25

Author(s):

Dragana Stanojevic ◽

Ljiljana Comic ◽

Olgica Stefanovic ◽

Slavica Solujic-Sukdolak

Keyword(s):

Antibacterial Activity ◽

Aqueous Extract ◽

Sodium Nitrite ◽

Sodium Benzoate ◽

Synergistic Action ◽

Salvia Officinalis ◽

Assay Method ◽

Potassium Sorbate ◽

Salvia Officinalis L

The aim of this work was to investigate the antibacterial activity of aqueous extracts of the species Salvia officinalis L. and its synergistic action with the preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergism was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/sodium benzoate, aqueous extract/potassium sorbate, aqueous extract/sodium nitrite combinations. Synergism was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergism was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values. Detected autoplagiarism. Link to the Editorial Decision <a href="http://dx.doi.org/10.2298/ABS1004251U">10.2298/ABS1004251U</a>

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Effects of some organic acids and salts on microbial fermentation in the digestive tract of piglets estimated using an in vitro gas production technique

Agricultural and Food Science ◽

10.2137/145960605775897687 ◽

2008 ◽

Vol 14 (4) ◽

pp. 311 ◽

Cited By ~ 5

Author(s):

K. PARTANEN ◽

T. JALAVA

Keyword(s):

Organic Acids ◽

Formic Acid ◽

Digestive Tract ◽

Sodium Benzoate ◽

Gas Production ◽

Control Treatment ◽

Potassium Sorbate ◽

Microbial Fermentation ◽

Calcium Formate

An in vitro gas production technique was used to screen different organic acids (formic, propionic, lactic, citric, and fumaric acid), organic salts (calcium formate, potassium sorbate, and sodium benzoate), and inorganic phosphoric acid for their ability to modulate microbial fermentation in the digestive tract of piglets. For the incubation, 40 ml of culture medium (53% buffer, 45% frozen ileal digesta, and 2% fresh faeces) was dispensed in vessels containing 5 ml of buffer, 0.5 g of feed, and 20 ìl of liquid or 20 mg of solid acidifiers. Gas production was measured every 15 min during the 24 h incubation at 39°C, and a Gompertz bacterial growth model was applied to the gas production data. Formic acid was the only acid that reduced the maximum rate of gas production (ìm) compared to that in the control treatment (P < 0.05). The ìm was slower in vessels with formic acid than in those with calcium formate, citric acid, and potassium sorbate (P < 0.05) Calcium formate increased the ìm compared to the control treatment (P < 0.05). The maximum volume of gas produced and the lag time did not differ between different acidifiers (P > 0.05). When investigating formic-acid-based mixtures that contained 15% of potassium sorbate and/or sodium benzoate, the estimated parameters for the Gompertz growth model did not differ from those for treatments with plain formic acid (P > 0.05). However, concentrations of total volatile fatty acids, acetic acid, propionic acid, and n-butyric acid were reduced by all the mixtures (P < 0.05), but not by plain formic acid (P > 0.05). In conclusion, organic acids and salts were found to differ in their ability to modulate microbial fermentation in the digestive tract of piglets. Mixing formic acid with potassium sorbate or sodium benzoate changed fermentation patterns, and the possibility to use them to enhance the antimicrobial effect of formic acid should be investigated further in vivo.;

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Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520150344 ◽

2016 ◽

Vol 88 (1) ◽

pp. 281-292 ◽

Cited By ~ 26

Author(s):

CHARLENE S.C. GARCIA ◽

CAROLINE MENTI ◽

ANA PAULA F. LAMBERT ◽

THIAGO BARCELLOS ◽

SIDNEI MOURA ◽

...

Keyword(s):

Mass Spectrometry ◽

Mammalian Cells ◽

Annexin V ◽

The Body ◽

Salvia Officinalis ◽

Gas Chromatography Mass Spectrometry ◽

Ionization Mass ◽

Hek 293 ◽

Salvia Officinalis L

ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.

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