In vitro synergistic antibacterial activity of Salvia officinalis L. and some preservatives

The aim of this work was to investigate the antibacterial activity of aqueous extracts of the species Salvia officinalis L. and its synergistic action with the preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergism was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/sodium benzoate, aqueous extract/potassium sorbate, aqueous extract/sodium nitrite combinations. Synergism was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergism was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values. Detected autoplagiarism. Link to the Editorial Decision <a href="http://dx.doi.org/10.2298/ABS1004251U">10.2298/ABS1004251U</a>

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Synergy between Salvia officinalis L. and some preservatives

Open Life Sciences ◽

10.2478/s11535-010-0026-0 ◽

2010 ◽

Vol 5 (4) ◽

pp. 491-495

Author(s):

Dragana Stanojević ◽

Ljiljana Čomić ◽

Olgica Stefanović

Keyword(s):

Aqueous Extract ◽

Sodium Nitrite ◽

Sodium Benzoate ◽

Synergistic Action ◽

Salvia Officinalis ◽

Assay Method ◽

Potassium Sorbate ◽

Checkerboard Assay ◽

Salvia Officinalis L

AbstractThe aim of the present study is to investigate the antibacterial activity of Salvia officinalis L. aqueous extracts and its synergistic action with preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergy was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/ sodium benzoate, aqueous extract/ potassium sorbate, aqueous extract/ sodium nitrite combinations. Synergy was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergy was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values.

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Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study

Anaerobe ◽

10.1016/j.anaerobe.2020.102194 ◽

2020 ◽

Vol 63 ◽

pp. 102194

Author(s):

Filipe Santos Ferreira Mendes ◽

Letícia Matanovich Garcia ◽

Thais da Silva Moraes ◽

Luciana Assirati Casemiro ◽

Clauber Barbosa de Alcântara ◽

...

Keyword(s):

Antibacterial Activity ◽

In Vitro Study ◽

Salvia Officinalis ◽

Vitro Study ◽

Salvia Officinalis L

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IN VITRO ANTAGONISTIC ACTIVITIES OF INDONESIAN MARINE SPONGE AAPTOS AAPTOS AND CALLYSPONGIA PSEUDORETICULATA EXTRACTS AND THEIR TOXICITY AGAINST Vibrio spp.

Indonesian Aquaculture Journal ◽

10.15578/iaj.6.2.2011.173-182 ◽

2011 ◽

Vol 6 (2) ◽

pp. 173 ◽

Cited By ~ 1

Author(s):

Rosmiati Rosmiati ◽

Habsah Mohamad ◽

Tengku Sifzizul Tengku Muhammad ◽

Najiah Musa ◽

Aziz Ahmad ◽

...

Keyword(s):

Antibacterial Activity ◽

Aqueous Extract ◽

Pathogenic Bacteria ◽

Vibrio Harveyi ◽

Diffusion Method ◽

Larval Rearing ◽

Butanol Extract ◽

Vibrio Spp ◽

Mic Value

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.

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Effects of some organic acids and salts on microbial fermentation in the digestive tract of piglets estimated using an in vitro gas production technique

Agricultural and Food Science ◽

10.2137/145960605775897687 ◽

2008 ◽

Vol 14 (4) ◽

pp. 311 ◽

Cited By ~ 5

Author(s):

K. PARTANEN ◽

T. JALAVA

Keyword(s):

Organic Acids ◽

Formic Acid ◽

Digestive Tract ◽

Sodium Benzoate ◽

Gas Production ◽

Control Treatment ◽

Potassium Sorbate ◽

Microbial Fermentation ◽

Calcium Formate

An in vitro gas production technique was used to screen different organic acids (formic, propionic, lactic, citric, and fumaric acid), organic salts (calcium formate, potassium sorbate, and sodium benzoate), and inorganic phosphoric acid for their ability to modulate microbial fermentation in the digestive tract of piglets. For the incubation, 40 ml of culture medium (53% buffer, 45% frozen ileal digesta, and 2% fresh faeces) was dispensed in vessels containing 5 ml of buffer, 0.5 g of feed, and 20 ìl of liquid or 20 mg of solid acidifiers. Gas production was measured every 15 min during the 24 h incubation at 39°C, and a Gompertz bacterial growth model was applied to the gas production data. Formic acid was the only acid that reduced the maximum rate of gas production (ìm) compared to that in the control treatment (P < 0.05). The ìm was slower in vessels with formic acid than in those with calcium formate, citric acid, and potassium sorbate (P < 0.05) Calcium formate increased the ìm compared to the control treatment (P < 0.05). The maximum volume of gas produced and the lag time did not differ between different acidifiers (P > 0.05). When investigating formic-acid-based mixtures that contained 15% of potassium sorbate and/or sodium benzoate, the estimated parameters for the Gompertz growth model did not differ from those for treatments with plain formic acid (P > 0.05). However, concentrations of total volatile fatty acids, acetic acid, propionic acid, and n-butyric acid were reduced by all the mixtures (P < 0.05), but not by plain formic acid (P > 0.05). In conclusion, organic acids and salts were found to differ in their ability to modulate microbial fermentation in the digestive tract of piglets. Mixing formic acid with potassium sorbate or sodium benzoate changed fermentation patterns, and the possibility to use them to enhance the antimicrobial effect of formic acid should be investigated further in vivo.;

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In vitro Study on Anti-salmonella Activities of Boerhaavia diffusa (L. syn) Leaf Extract

International Journal of Pathogen Research ◽

10.9734/ijpr/2019/v3i130081 ◽

2019 ◽

pp. 1-10

Author(s):

Ologun, Oluwabusayo ◽

Dada, Ebenezer Oluyemi ◽

Ajayi, Kehinde Oluyemi

Keyword(s):

Antibacterial Activity ◽

Clinical Isolate ◽

Optical Density ◽

Aqueous Extract ◽

Leaf Extract ◽

Ethanol Extract ◽

Salmonella Typhi ◽

Boerhaavia Diffusa ◽

Broth Dilution

Various strategies have been employed in the treatment and management of Salmonella infection however, Salmonella strains have gained resistance to antibiotics. This study was to determine in vitro anti-Salmonella activity of Boerhaavia diffusa leaf extract against clinical isolate of Salmonella typhi and Salmonella typhi ATCC 14028. The aqueous and ethanol extracts of B. diffusa were studied for their antibacterial activity against pathogenic Salmonella typhi. This study was carried out between April and September 2018. The in vitro antibacterial activity was performed by agar well diffusion method and broth dilution using spectrophotometric method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well and optical density respectively. It was observed that aqueous extract exerted slightly higher activity than ethanolic extract as revealed by the mean diameter of zone of inhibitions at a concentration of 200 mg/ml, the aqueous extract had 35.21±0.47 mm (Salmonella typhi ATCC 14028) compared with ethanol extract 26.41±0.32 mm (clinical). However, in the broth dilution method, ethanol extract significantly (p=0.05) reduced the cell, at 48 hours, the optical density of clinical isolate of S. typhi treated at concentration of 200 mg/ml of extract was 0.47±0.02 nm while at the same concentration of extract, aqueous extract had an optical density of 0.52±0.11 nm respectively. The phytochemical assay revealed that tannin (5.18±0.02 mg/g) and quinone (8.45±0.13 mg/g) in ethanol extract was significantly (p=0.05) higher than aqueous extract while saponin (14.18±0.06 mg/g) was higher in the aqueous extract. The ethanol and aqueous extracts of leaves of B. diffusa whole plant exhibited significant antibacterial activity against both clinical and typed Salmonella typhi. Therefore, the plant extract could be used for the treatment of Salmonellosis, however, the in vivo studies is needed to ascertain the safety of the extract.

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Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520150344 ◽

2016 ◽

Vol 88 (1) ◽

pp. 281-292 ◽

Cited By ~ 26

Author(s):

CHARLENE S.C. GARCIA ◽

CAROLINE MENTI ◽

ANA PAULA F. LAMBERT ◽

THIAGO BARCELLOS ◽

SIDNEI MOURA ◽

...

Keyword(s):

Mass Spectrometry ◽

Mammalian Cells ◽

Annexin V ◽

The Body ◽

Salvia Officinalis ◽

Gas Chromatography Mass Spectrometry ◽

Ionization Mass ◽

Hek 293 ◽

Salvia Officinalis L

ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.

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