Production and application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in acquisition of glucose

AbstractAmylases from Rhizopus oryzae and Rhizopus microsporus var. oligosporus were obtained using agro-industrial wastes as substrates in submerged batch cultures. The enzymatic complex was partially characterised for use in the production of glucose syrup. Type II wheat flour proved better than cassava bagasse as sole carbon source for amylase production. The optimum fermentation condition for both microorganisms was 96 hours at 30°C and the amylase thus produced was used for starch hydrolysis. The product of the enzymatic hydrolysis indicated that the enzyme obtained was glucoamylase, only glucose as final product was attained for both microorganisms. R. oligosporus was of greater interest than R. oryzae for amylase production, taking into account enzyme activity, cultivation time, thermal stability and pH range. Glucose syrup was produced using concentrated enzyme and 100 g L−1 starch in a 4 hours reaction at 50°C. The bioprocess studied can contribute to fungus glucoamylase production and application.

Download Full-text

Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest

Biota Neotropica ◽

10.1590/1676-0611-bn-2017-0337 ◽

2017 ◽

Vol 17 (3) ◽

Cited By ~ 6

Author(s):

Paula Zaghetto de Almeida ◽

Marita Gimenez Pereira ◽

Caio Cesar de Carvalho ◽

Paulo Ricardo Heinen ◽

Luciana Sobrani Ziotti ◽

...

Keyword(s):

Filamentous Fungi ◽

Atlantic Forest ◽

Rhizopus Oryzae ◽

Residual Activity ◽

Brazilian Atlantic Forest ◽

Tropical Environments ◽

Aspergillus Brasiliensis ◽

Significant Difference ◽

Initial Ph ◽

Ph Range

Abstract Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments - which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great amounts of glucoamylases. The enzymes of A. brasiliensis and R. oryzae are different, possibly because of their phylogenetical distance. The best amylase production of A. brasiliensis occurred during 120 hours with initial pH of 7.5; it had a better activity in the pH range of 3.5-5.0 and at 60-75°C. Both fungal glucoamylase had wide pH stability (3-8) and were activated by Mn2+. R. oryzae best production occurred in 96 hours and at pH 6.5. Its amylases had a greater activity in the pH range of 4.0-5.5 and temperature at 50-65ºC. The most significant difference between the enzymes produced by both fungi is the resistance to thermal denaturation: A. brasiliensis glucoamylase had a T50 of 60 minutes at 70ºC. The R. oryzae glucoamylase only had a residual activity when incubated at 50°C with a 12 min T50.

Download Full-text

Truncated Prosequence of Rhizopus oryzae Lipase: Key Factor for Production Improvement and Biocatalyst Stability

Catalysts ◽

10.3390/catal9110961 ◽

2019 ◽

Vol 9 (11) ◽

pp. 961 ◽

Cited By ~ 2

Author(s):

Josu López-Fernández ◽

Juan J. Barrero ◽

M. Dolors Benaiges ◽

Francisco Valero

Keyword(s):

Amino Acids ◽

Ionic Strength ◽

Rhizopus Oryzae ◽

Lipase Production ◽

Biochemical Properties ◽

Mature Sequence ◽

Rhizopus Oryzae Lipase ◽

Batch Cultures ◽

Production Improvement ◽

Key Factor

Recombinant Rhizopus oryzae lipase (mature sequence, rROL) was modified by adding to its N-terminal 28 additional amino acids from the C-terminal of the prosequence (proROL) to obtain a biocatalyst more suitable for the biodiesel industry. Both enzymes were expressed in Pichia pastoris and compared in terms of production bioprocess parameters, biochemical properties, and stability. Growth kinetics, production, and yields were better for proROL harboring strain than rROL one in batch cultures. When different fed-batch strategies were applied, lipase production and volumetric productivity of proROL-strain were always higher (5.4 and 4.4-fold, respectively) in the best case. rROL and proROL enzymatic activity was dependent on ionic strength and peaked in 200 mM Tris-HCl buffer. The optimum temperature and pH for rROL were influenced by ionic strength, but those for proROL were not. The presence of these amino acids altered lipase substrate specificity and increased proROL stability when different temperature, pH, and methanol/ethanol concentrations were employed. The 28 amino acids were found to be preferably removed by proteases, leading to the transformation of proROL into rROL. Nevertheless, the truncated prosequence enhanced Rhizopus oryzae lipase heterologous production and stability, making it more appropriate as industrial biocatalyst.

Download Full-text

Data base simulation of batch and fed-batch cultures for α-amylase production using a culture data base and a statistical procedure

Journal of Fermentation and Bioengineering ◽

10.1016/0922-338x(93)90203-k ◽

1993 ◽

Vol 76 (4) ◽

pp. 326-332 ◽

Cited By ~ 9

Author(s):

Jun-Ichi Horiuchi ◽

Michimasa Kishimoto ◽

Masami Kamasawa ◽

Hisashi Miyakawa ◽

Haruo Momose

Keyword(s):

Data Base ◽

Statistical Procedure ◽

Fed Batch ◽

Batch Cultures ◽

Amylase Production

Download Full-text

Influence of Environmentally Friendly and High-Efficiency Composite Additives on Pulverized Coal Combustion in Cement Industry

Journal of Combustion ◽

10.1155/2016/8205945 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Zhiyong Wang ◽

Shijie Wang ◽

Qi Zhang ◽

Qingyan Fang ◽

Wangsheng Chen

Keyword(s):

High Efficiency ◽

Combustion Process ◽

Combustion Efficiency ◽

Cement Industry ◽

Industrial Wastes ◽

Chemical Reagents ◽

Cold Rolled ◽

Burnout Rate ◽

The Cost ◽

Better Than

4 kinds of chemical reagents and 3 kinds of industrial wastes were selected as burning additives for 2 kinds of coals in cement industry. The work focused on the replacement of partial chemical reagents by industrial wastes, which not only reduced the cost and took full advantage of industrial wastes, but also guaranteed the high combustion efficiency and removed theNOXand SO2simultaneously. The experiments were carried out in DTF. The combustion residues were analyzed by SEM and XRD. The results showed that the burnout rate was increased after adding the additives; meanwhile, theNOXand SO2release concentration were reduced, but the degree of action varied for different additives and coals. The substitute of chemical reagents by industrial wastes was very effective; overall, the cold-rolled iron oxide worked better than others; the particles surface was tougher and the peaks of crystalline phase were lower than raw coal, which indicated that the additives played good roles in combustion process.

Download Full-text

Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822011000400014 ◽

2011 ◽

Vol 42 (4) ◽

pp. 1334-1342 ◽

Cited By ~ 25

Author(s):

Rajshree Saxena ◽

Rajni Singh

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Industrial Wastes ◽

Bacillus Sp ◽

Amylase Production

Download Full-text

Usefulness of Activated Carbon Prepared from Industrial Wastes in the Removal of Nickel from Aqueous Solution

E-Journal of Chemistry ◽

10.1155/2009/632012 ◽

2009 ◽

Vol 6 (2) ◽

pp. 361-370 ◽

Cited By ~ 4

Author(s):

R. Rajalakshmi ◽

S. Subhashini ◽

P. Lalitha

Keyword(s):

Aqueous Solution ◽

Industrial Wastes ◽

Polluted Water ◽

First Order Kinetics ◽

Successful Removal ◽

Ph Range ◽

Direct Proportionality ◽

By Products ◽

Maximum Removal ◽

Nickel Adsorption

Elimination of heavy metals like nickel from waste water is an important subject in view of public health. In the present study, an attempt has been made to study the applicability of industrial by-products as potential metal adsorbents to remove nickel from aqueous solutions and polluted water. A direct proportionality between the percentage of Ni(II) removal and adsorbent dosage was noted. Maximum removal ⁄ recovery of nickel was achieved at pH range of 10-12 for all adsorbents. An optimum temperature of 40 °C for efficient removal of Ni(II) was observed. The effect of nickel adsorption was affected by salinity. The adsorption isotherm data confirmed to Freundlich and Langmuir isotherms. Conformation of data to the Lagergren᾽s rate equation indicated first order kinetics. The suitability of the industrial by-products in the successful removal of nickel from aqueous solution is quite obvious from the study.

Download Full-text

Analysis of the Bacterial Sulphur System

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.825.190 ◽

2013 ◽

Vol 825 ◽

pp. 190-193

Author(s):

Rachel M. Candy ◽

Kyle R. Blight ◽

David E. Ralph

Keyword(s):

Bacterial Cell ◽

Mine Drainage ◽

Solid Phase ◽

Low Grade ◽

Ph Measurements ◽

Batch Cultures ◽

Partial Pressures ◽

S Oxidation ◽

Ph Range

Heterogeneous bacterial sulphur systems are inherently complicated. However, developing an understanding of the influence of environmental factors such as pH,Iand PCO2is important for a number of fields. Examples of these include minimising acid mine drainage and maximising metal recovery from low-grade sulphide minerals. Measuring the effect of these factors on the extent and rate of sulphur (S) oxidation is complicated by the presence and nature of solid phase elemental S. The rate and extent of S oxidation can be determined indirectly via the reaction product, H2SO4, which was quantified using pH measurements in this study. The method was critically dependent on the quality of pH data but proved effective in providing rate constants for the catalysed S oxidation reaction and yield (biomass/substrate) estimates in the range pH > 1.5. IncreasingIover the range 0.176 0.367 mol L-1decreased bacterial cell yields but increased the rate of sulphur oxidation significantly. Partial pressures of CO2in the range of 0.039 1.18% v/v produced no significant effect on the rates of S oxidation or bacterial cell yields. Bacterial cell yields were not affected in the pH range 1.5 2.5, however the rate of S oxidation increased significantly from pH 2.0 2.5. In the range pH < 1.5 the batch cultures progressed and although no reliable rate data was recorded cell yields decreased from 7.43 to 2.05 (× 1012cells mol-1) at pH 1.5 to 1.0 respectively.

Download Full-text

Searching the best operational strategies for Rhizopus oryzae lipase production in Pichia pastoris Mut+ phenotype: Methanol limited or methanol non-limited fed-batch cultures?

Biochemical Engineering Journal ◽

10.1016/j.bej.2013.03.018 ◽

2013 ◽

Vol 75 ◽

pp. 47-54 ◽

Cited By ~ 29

Author(s):

José Manuel Barrigón ◽

José Luis Montesinos ◽

Francisco Valero

Keyword(s):

Pichia Pastoris ◽

Rhizopus Oryzae ◽

Lipase Production ◽

Fed Batch ◽

Rhizopus Oryzae Lipase ◽

Operational Strategies ◽

Batch Cultures

Download Full-text

Optimization of Amylase Production fromB. amyloliquefaciens(MTCC 1270) Using Solid State Fermentation

International Journal of Microbiology ◽

10.1155/2014/764046 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Koel Saha ◽

Sujan Maity ◽

Sudeshna Roy ◽

Koustav Pahan ◽

Rishija Pathak ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Wide Spectrum ◽

Chloride Ions ◽

Fermentation Condition ◽

Structure Activity ◽

Agroindustrial Waste ◽

Incubation Periods ◽

Amylase Production ◽

Microbial Strain

Demand for microbial amylase production persists because of its immense importance in wide spectrum industries. The present work has been initiated with a goal of optimization of solid state fermentation condition for amylase using agroindustrial waste and microbial strain likeB. amyloliquefaciens(MTCC 1270). In an aim to improve the productivity of amylase, fermentation has been carried out in the presence of calcium (Ca+2), Nitrate (NO3−), and chloride ions (Cl−) as well as in the presence of D-inositol and mannitol. Amylase needs calcium ion for the preservation of its structure, activity and stability that proves beneficial also for amylase production using solid state fermentation. The inclusion of ions and sugars in the SSF media is promising which can be explained by the protection offered by them against thermal decay of amylase at various incubation periods at 37°C.

Download Full-text