Cloning of taxane 2α-O-benzoyltransferase (TBT) genomic DNA from Taxus

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Dongli Zhao ◽  
Le Li ◽  
Qian Wang ◽  
Guoyin Kai ◽  
Xiang Wang ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Cdna Sequence ◽  
Gene Diversity ◽  
Genomic Sequences ◽  
Taxus Yunnanensis ◽  
Sequence Encoding ◽  
First Time

AbstractBased on the cDNA sequence encoding taxane 2α-O-benzoyltransferase (TBT) from Taxus yunnanensis (Gen-Bank Accession No.: AY970522), genomic sequences of TBTs from T. yunnanensis (TyTBT) and T. cuspidata (TcuTBT) were cloned for the first time. They both contain only one intron. The finding that the introns of TyTBT and TcuTBT are more diverse than their exons implies that the gene diversity is more within introns than within exons, which may be important for keeping the functions of the genes.

scholarly journals Analysis of HLA-G long-read genomic sequences in mother–offspring pairs with preeclampsia

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayako Nishizawa ◽  
Kazuki Kumada ◽  
Keiko Tateno ◽  
Maiko Wagata ◽  
Sakae Saito ◽  
...  
Keyword(s):  
Single Molecule ◽  
Gene Polymorphisms ◽  
Genomic Dna ◽  
Genomic Sequences ◽  
Genomic Sequencing ◽  
Public Database ◽  
Coding Sequences ◽  
Pacbio Rs Ii ◽  
Potential Association ◽  
Long Read

AbstractPreeclampsia is a pregnancy-induced disorder that is characterized by hypertension and is a leading cause of perinatal and maternal–fetal morbidity and mortality. HLA-G is thought to play important roles in maternal–fetal immune tolerance, and the associations between HLA-G gene polymorphisms and the onset of pregnancy-related diseases have been explored extensively. Because contiguous genomic sequencing is difficult, the association between the HLA-G genotype and preeclampsia onset is controversial. In this study, genomic sequences of the HLA-G region (5.2 kb) from 31 pairs of mother–offspring genomic DNA samples (18 pairs from normal pregnancies/births and 13 from preeclampsia births) were obtained by single-molecule real-time sequencing using the PacBio RS II platform. The HLA-G alleles identified in our cohort matched seven known HLA-G alleles, but we also identified two new HLA-G alleles at the fourth-field resolution and compared them with nucleotide sequences from a public database that consisted of coding sequences that cover the 3.1-kb HLA-G gene span. Intriguingly, a potential association between preeclampsia onset and the poly T stretch within the downstream region of the HLA-G*01:01:01:01 allele was found. Our study suggests that long-read sequencing of HLA-G will provide clues for characterizing HLA-G variants that are involved in the pathophysiology of preeclampsia.

cDNA sequence, mRNA expression and genomic DNA of trypsinogen from the Indianmeal moth, Plodia interpunctella

2000 ◽  
Vol 9 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Y. C. Zhu ◽  
B. Oppert ◽  
K. J. Kramer ◽  
W. H. McGaughey ◽  
A. K. Dowdy
Keyword(s):  
Mrna Expression ◽  
Genomic Dna ◽  
Cdna Sequence ◽  
Plodia Interpunctella ◽  
Indianmeal Moth

scholarly journals Partial cDNA sequence encoding a nuclear pore protein modified by O-linked N-acetylglucosamine.

1988 ◽  
Vol 85 (24) ◽  
pp. 9595-9599 ◽  
Author(s):  
M. D'Onofrio ◽  
C. M. Starr ◽  
M. K. Park ◽  
G. D. Holt ◽  
R. S. Haltiwanger ◽  
...  
Keyword(s):  
Cdna Sequence ◽  
Nuclear Pore ◽  
Nuclear Pore Protein ◽  
Partial Cdna Sequence ◽  
Partial Cdna ◽  
Sequence Encoding ◽  
Pore Protein

Aureobasidium pullulans Gene from Egypt

2021 ◽  
Vol 8 (1) ◽  
pp. 6-10
Author(s):  
Hani Moubasher ◽  
Salwa S Wahsh ◽  
Nabil Abo El-Kassem ◽  
Refaat Ali
Keyword(s):  
Molecular Mass ◽  
Genomic Dna ◽  
Aureobasidium Pullulans ◽  
Hypothetical Protein ◽  
Significant Alignment ◽  
First Time

Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.

scholarly journals Analysis of the cDNA sequence encoding MHC-Aβ in tubular epithelium from mouse kidney

1991 ◽  
Vol 40 (1) ◽  
pp. 139-142 ◽  
Author(s):  
Shelley E. Albert ◽  
Kathleen Shelton ◽  
Mae Jane Sun ◽  
Eric G. Neilson
Keyword(s):  
Cdna Sequence ◽  
Mouse Kidney ◽  
Tubular Epithelium ◽  
Sequence Encoding

scholarly journals Gene prediction by multiple syntenic alignment

2005 ◽  
Vol 2 (1) ◽  
pp. 38-47
Author(s):  
Said S. Adi ◽  
Carlos E. Ferreira
Keyword(s):  
Computer Program ◽  
Genomic Dna ◽  
Gene Prediction ◽  
Genomic Sequences ◽  
Prediction Problem ◽  
Huge Amount ◽  
Protein Coding ◽  
Coding Regions ◽  
Conserved Regions ◽  
Similarity Information

Summary Given the increasing number of available genomic sequences, one now faces the task of identifying their functional parts, like the protein coding regions. The gene prediction problem can be addressed in several ways. One of the most promising methods makes use of similarity information between the genomic DNA and previously annotated sequences (proteins, cDNAs and ESTs). Recently, given the huge amount of newly sequenced genomes, new similarity-based methods are being successfully applied in the task of gene prediction. The so-called comparative-based methods lie in the similarities shared by regions of two evolutionary related genomic sequences. Despite the number of different gene prediction approaches in the literature, this problem remains challenging. In this paper we present a new comparative-based approach to the gene prediction problem. It is based on a syntenic alignment of three or more genomic sequences. With syntenic alignment we mean an alignment that is constructed taking into account the fact that the involved sequences include conserved regions intervened by unconserved ones. We have implemented the proposed algorithm in a computer program and confirm the validity of the approach on a benchmark including triples of human, mouse and rat genomic sequences.

scholarly journals Complete cDNA sequence encoding human β-galactoside α-2, 6-sialyltransferase

1990 ◽  
Vol 18 (3) ◽  
pp. 667-667 ◽  
Author(s):  
Ulrich Grundmann ◽  
Claudia Nerlich ◽  
Thomas Rein ◽  
Gerd Zettlmeissl
Keyword(s):  
Cdna Sequence ◽  
Sequence Encoding

scholarly journals Genetic study of motor functions in Drosophila melanogaster

2012 ◽  
Vol 10 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Sergey A Fedotov ◽  
Julia V Bragina ◽  
Nataliya G Besedina ◽  
Larisa V Danilenkova ◽  
Elena A Kamysheva ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Motor Activity ◽  
Genomic Dna ◽  
Molecular Mechanisms ◽  
Central Pattern Generators ◽  
Courtship Song ◽  
P Element ◽  
Rhythmic Motor ◽  
Pattern Generators ◽  
First Time

To investigate molecular mechanisms of central pattern generators (CPG s) functioning, we carried out a screening of collection of Drosophila P-insertional mutants for strong deviations in locomotion and courtship song. In 21 mutants, the site of the P-insertion was localized by sequencing of the fragments of genomic DNA flanking the P-element. Bioinformational analysis revealed a list of candidate genes, potential players in development and functioning of CPG s. Possible involvement of certain identified genes in rhythmic motor activity is suggested for the first time (CG15630, Map205).

Sign in / Sign up

Export Citation Format

Share Document