Aureobasidium pullulans Gene from Egypt

2021 ◽  
Vol 8 (1) ◽  
pp. 6-10
Author(s):  
Hani Moubasher ◽  
Salwa S Wahsh ◽  
Nabil Abo El-Kassem ◽  
Refaat Ali
Keyword(s):  
Molecular Mass ◽  
Genomic Dna ◽  
Aureobasidium Pullulans ◽  
Hypothetical Protein ◽  
Significant Alignment ◽  
First Time

Sequancing of pullulanase from the fungus Aureobasidium pullulans isolated from Egypt soil; Genomic DNA of pullulanase was determined for the first time using PCR, according to Baser program, Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed similarity using NCBI significant alignment with Aureobasidium namibiae CBS 147.97 hypothetical protein partial mRNA and 46 % with Aureobasidium pullulans JQ624241 and AF470619; Identified sequenced fragment was 2051 bp. and G+C content is 50.5% with molecular mass 63 KDa.

Cloning of taxane 2α-O-benzoyltransferase (TBT) genomic DNA from Taxus

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Dongli Zhao ◽  
Le Li ◽  
Qian Wang ◽  
Guoyin Kai ◽  
Xiang Wang ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Cdna Sequence ◽  
Gene Diversity ◽  
Genomic Sequences ◽  
Taxus Yunnanensis ◽  
Sequence Encoding ◽  
First Time

AbstractBased on the cDNA sequence encoding taxane 2α-O-benzoyltransferase (TBT) from Taxus yunnanensis (Gen-Bank Accession No.: AY970522), genomic sequences of TBTs from T. yunnanensis (TyTBT) and T. cuspidata (TcuTBT) were cloned for the first time. They both contain only one intron. The finding that the introns of TyTBT and TcuTBT are more diverse than their exons implies that the gene diversity is more within introns than within exons, which may be important for keeping the functions of the genes.

scholarly journals Genetic study of motor functions in Drosophila melanogaster

2012 ◽  
Vol 10 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Sergey A Fedotov ◽  
Julia V Bragina ◽  
Nataliya G Besedina ◽  
Larisa V Danilenkova ◽  
Elena A Kamysheva ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Motor Activity ◽  
Genomic Dna ◽  
Molecular Mechanisms ◽  
Central Pattern Generators ◽  
Courtship Song ◽  
P Element ◽  
Rhythmic Motor ◽  
Pattern Generators ◽  
First Time

To investigate molecular mechanisms of central pattern generators (CPG s) functioning, we carried out a screening of collection of Drosophila P-insertional mutants for strong deviations in locomotion and courtship song. In 21 mutants, the site of the P-insertion was localized by sequencing of the fragments of genomic DNA flanking the P-element. Bioinformational analysis revealed a list of candidate genes, potential players in development and functioning of CPG s. Possible involvement of certain identified genes in rhythmic motor activity is suggested for the first time (CG15630, Map205).

Isolation and characterization of chitinases from Verticillium lecanii

2005 ◽  
Vol 51 (12) ◽  
pp. 1045-1055 ◽  
Author(s):  
Zhen-Xiang Lu ◽  
André Laroche ◽  
Hung Chang Huang
Keyword(s):  
Molecular Mass ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Verticillium Lecanii ◽  
Phylogenetic Groups ◽  
Sequence Alignments ◽  
Chitin Binding Domain ◽  
Isolation And Characterization ◽  
Fungal Chitinases

Degenerate PCR primers corresponding to conserved domains of fungal chitinases were designed, and PCR was performed on genomic DNA of the entomogenous fungus Verticillium lecanii (Zimmermann) Viegas. Two distinct PCR fragments, chf1 and chf2, were isolated and used to identify two DNA contigs. Analyses of these two contigs revealed that we had obtained the full-length DNA sequence including the promoter, 5′ untranslated region, open reading frame (ORF), and 3′ untranslated regions for two distinct chitinase-like genes. These two genomic DNA sequences exhibited 51% identity at the amino acid (aa) level and were designed as acidic (chi1) and basic (chi2) chitinase-like genes. The isolated cDNA for chi1 gene is 1110 bp with a predicted protein of 370 aa and molecular mass of 40.93 kDa, and its ORF was uninterrupted in its corresponding genomic DNA sequence. The cDNA for the chi2 gene is 1269 bp, a predicted ORF of 423 aa and molecular mass of 45.95 kDa. In contrast, the ORF was interrupted by three introns in its corresponding genomic DNA. The basic chitinase gene (chi2) was successfully expressed in the Pichia pastoris system; optimum enzymatic activity was observed at 22 °C and at pH 7.5. CHI1 and CHI2 were clustered into two different phylogenetic groups according to their sequence alignments with 28 other fungal chitinases. A chitin-binding domain, comprising two sub-domains that exhibit similarities at the aa level to chitin binding domains in bacteria, was identified in 30 fungal chitinase sequences examined.Key words: fungus, chitin, cloning, sequencing, transformation, Pichia sp. expression.

scholarly journals Pseudomonas aeruginosa Requires the DNA-Specific Endonuclease EndA To Degrade Extracellular Genomic DNA To Disperse from the Biofilm

2019 ◽  
Vol 201 (18) ◽  
Author(s):  
Kathryn E. Cherny ◽  
Karin Sauer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Genomic Dna ◽  
Early Stage ◽  
Content Type ◽  
Biofilm Dispersion ◽  
Biofilm Structure ◽  
First Time ◽  
Biofilm Matrix ◽  
Dispersed Cells

ABSTRACT The dispersion of biofilms is an active process resulting in the release of planktonic cells from the biofilm structure. While much is known about the process of dispersion cue perception and the subsequent modulation of the c-di-GMP pool, little is known about subsequent events resulting in the release of cells from the biofilm. Given that dispersion coincides with void formation and an overall erosion of the biofilm structure, we asked whether dispersion involves degradation of the biofilm matrix. Here, we focused on extracellular genomic DNA (eDNA) due to its almost universal presence in the matrix of biofilm-forming species. We identified two probable nucleases, endA and eddB, and eddA encoding a phosphatase that were significantly increased in transcript abundance in dispersed cells. However, only inactivation of endA but not eddA or eddB impaired dispersion by Pseudomonas aeruginosa biofilms in response to glutamate and nitric oxide (NO). Heterologously produced EndA was found to be secreted and active in degrading genomic DNA. While endA inactivation had little effect on biofilm formation and the presence of eDNA in biofilms, eDNA degradation upon induction of dispersion was impaired. In contrast, induction of endA expression coincided with eDNA degradation and resulted in biofilm dispersion. Thus, released cells demonstrated a hyperattaching phenotype but remained as resistant to tobramycin as biofilm cells from which they egress, indicating EndA-dispersed cells adopted some but not all of the phenotypes associated with dispersed cells. Our findings indicate for the first time a role of DNase EndA in dispersion and suggest weakening of the biofilm matrix is a requisite for biofilm dispersion. IMPORTANCE The finding that exposure to DNase I impairs biofilm formation or leads to the dispersal of early stage biofilms has led to the realization of extracellular genomic DNA (eDNA) as a structural component of the biofilm matrix. However, little is known about the contribution of intrinsic DNases to the weakening of the biofilm matrix and dispersion of established biofilms. Here, we demonstrate for the first time that nucleases are induced in dispersed Pseudomonas aeruginosa cells and are essential to the dispersion response and that degradation of matrix eDNA by endogenously produced/secreted EndA is required for P. aeruginosa biofilm dispersion. Our findings suggest that dispersing cells mediate their active release from the biofilm matrix via the induction of nucleases.

Darstellung von Peroxotantalaten durch Perhydrolyse von Tantal(V)-äthanolat / Preparation of Peroxotantalates by Perhydrolysis of Tantalum(V)ethoxide

1973 ◽  
Vol 28 (9-10) ◽  
pp. 590-594 ◽  
Author(s):  
J. Fuchs ◽  
D. Lubkoll
Keyword(s):  
Petroleum Ether ◽  
Molecular Mass ◽  
Water Soluble ◽  
Organic Cations ◽  
Water Soluble Compound ◽  
Soluble Compound ◽  
First Time

Tetraperoxotantalates with organic cations as well as an oligomeric peroxopolytantalate were prepared for the first time by perhydrolysis of tantalum(V)ethoxide, Ta(OC2H5)5, in the presence of bases. Guanidinium, tert-butylammonium and cyclohexylammonium salts of tetraperoxotantalic acid crystallize from ethanol as relatively stable compounds. The cell constants of these compounds are determined. By perhydrolysis of the ester in petroleum ether the tert-butylammonium salt of a peroxododecantalic acid was obtained. The molecular mass of this water soluble compound was determined with the ultracentrifuge.

scholarly journals Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill).

2013 ◽  
Vol 60 (1) ◽  
Author(s):  
Mariola Galbas ◽  
Filip Porzucek ◽  
Anna Woźniak ◽  
Ryszard Słomski ◽  
Marek Selwet
Keyword(s):  
Cell Proliferation ◽  
Glycine Max ◽  
Molecular Mass ◽  
Molecular Level ◽  
Cancer Cell Proliferation ◽  
Isolation And Purification ◽  
Anticancer Properties ◽  
Glycine Max L ◽  
First Time ◽  
Proteins And Peptides

Numerous studies have shown that consumption of soybean products decrease the risk of cancers in humans. Experiments at the molecular level have demonstrated that in most cases proteins and peptides are responsible for the anticancer properties of soybeen. Special attention should be paid to lunasin - a peptide described for the first time 16 years ago. Due to its structure it causes i.a., inhibition of cancer cell proliferation. A novel procedure for the isolation and purification of low-molecular-mass 2S soybean albumin protein is described in the present paper. A fraction of four peptides one of them corresponding to molecular mass and isoelectric point characteristic for lunasin. The obtained peptides decreased on the rate of HeLa cell proliferation.

First molecular detection and characterization of Sarcocystis infection in naturally infected buffaloes, Brazil

2020 ◽  
Author(s):  
Luiza Pires Portella ◽  
Fagner D'ambroso Fernandes ◽  
Camila Encarnação Minuzzi ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Fernando Vilani de Pelegrini ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Genomic Dna ◽  
Molecular Detection ◽  
Natural Infection ◽  
Sarcocystis Species ◽  
Sarcocystis Spp ◽  
The World ◽  
First Time ◽  
Pathogenic Effects

Sarcocystosis is a disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in ruminants causing pathogenic effects. Although the distribution of Sarcocystis can be found all over the world, the species infecting buffaloes in Brazil is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in buffaloes using molecular identification. In addition, phylogenetic analyzes were used for the first time to identify the different species of this protozoan infecting buffalo in the south of the country. Heart samples from 80 buffaloes were subjected to microscopic examination, followed by molecular analysis. Microcysts were present in 19/80 (23,75%) of the samples. Genomic DNA was extracted from the 19 isolates, all there were amplified DNA in the primer used in the study. Six readable sequences were obtained after sequencing of the samples in both the directions. In the present study all the sequenced samples indicated were of Sarcocystis levinei.

Phylogenetic analysis of wood-inhabiting molds and assessment of soft-rot wood deterioration. Part 5. Genus Aureobasidium

Holzforschung ◽  
2017 ◽  
Vol 71 (5) ◽  
pp. 437-443
Author(s):  
Young Min Lee ◽  
Hanbyul Lee ◽  
Young Mok Heo ◽  
Joo-Hyun Hong ◽  
Seokyoon Jang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Aureobasidium Pullulans ◽  
Soft Rot ◽  
Wood Degradation ◽  
Degradation Pattern ◽  
Wood Deterioration ◽  
First Time ◽  
Aureobasidium Melanogenum ◽  
Scanning Electron

Abstract The genus Aureobasidium is wellknown as a wood-staining mold and as a black yeast-like fungi, which produces mainly dark spores or pigmented hyphae within the wood cell lumens. Nevertheless, few studies are dedicated to wood-colonizing Aureobasidium species and little is known about the wood degradation patterns of this genus. In the present study, four Aureobasidium species, including Aureobasidium melanogenum, Aureobasidium leucospermi, Aureobasidium pullulans, and an unknown Aureobasidium sp., were isolated and identified based on phylogenetic analysis. A. melanogenum and A. leucospermi were observed for the first time in Korea. The degradation pattern of Douglas-fir by Aureobasidium was observed for the first time by scanning electron microscopy (SEM). All tested Aureobasidium species except an unknown Aureobasidium sp. revealed soft-rot Type ΙΙ (erosion) in sapwood pine.

scholarly journals A novel emaravirus identified in maple with leaf mottle symptoms by deep sequencing

2020 ◽  
Author(s):  
Artemis Rumbou ◽  
Thierry Candresse ◽  
Susanne von Bargen ◽  
Carmen Büttner
Keyword(s):  
Sequence Data ◽  
Hypothetical Protein ◽  
Acer Pseudoplatanus ◽  
Rna Seq ◽  
Glycoprotein Precursor ◽  
Putative Movement Protein ◽  
Symptomatic Sample ◽  
Viral Replicase ◽  
Full Length Genome ◽  
First Time

AbstractThe full-length genome of a novel Emaravirus has been identified and characterized from sycamore maple (Acer pseudoplatanus) - a tree species of significant importance in urban and forest areas - showing leaf mottle symptoms. RNA-Seq was performed using RNA preparations from a symptomatic and a symptomless maple tree. Purified double-stranded cDNA from each sample were used for RNA-Seq analysis on the Illumina HiSeq2500system and 14-198 MB data/sample of 100 bp-long paired-end sequence reads were generated. The sequence assembly and analysis revealed the presence of six RNA segments in the symptomatic sample (RNA1: 7,075 nt-long encoding the viral replicase; RNA2: 2,289 nt-long encoding the glycoprotein precursor; RNA3: 1,525 nt-long encoding the nucleocapsid protein; RNA4: 1,533 nt-long encoding the putative movement protein; RNA5: 1,825 nt-long encoding a hypothetical protein P5; RNA6: 1,179 nt-long encoding a hypothetical protein P6). Two independent HTS sequencing runs from the same symptomatic maple tree detected the same genome segments. For one of these sequencing runs the cDNA library was prepared using a primer targeting the conserved genome terminal region, known to be shared between emaraviruses genome segments and a high amount of sequence data was generated. We suggest, therefore, that the six identified genome segments represent the complete genome of a novel emaravirus from maple, which we tentatively name maple mottle-associated virus (MaMaV). RT-PCR assays were performed on symptomatic and non-symptomatic leaves of A. pseudoplatanus trees coming growing on two different locations in Berlin. MaMaV was only detected from symptomatic trees and all six RNAs were generally simultaneously detected. Non-symptomatic samples were consistently negative for MaMaV. These results suggest that MaMaV might be the symptom inducing virus in the sampled trees. In the present state of the art, this is the first time an Emaravirus is described from maple and is fully genetically characterized.

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