Expression pattern of PsAPY1 during apical hook development in pea

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Trivima Sharma ◽  
Eugene Morita ◽  
Shunnosuke Abe
Keyword(s):  
Expression Pattern ◽  
Shoot Meristem ◽  
Temporal Expression ◽  
Transcript Accumulation ◽  
Essential Information ◽  
Apical Hook ◽  
Mrna In Situ Hybridization ◽  
Spatio Temporal ◽  
Hydrolysis Of

AbstractApyrase (ATP diphosphohydrolase, EC 3.6.1.5) catalyzes hydrolysis of nucleoside tri- and di-phosphates to nucleoside monophosphates and orthophosphates. In the present study, the spatio-temporal expression of an apyrase gene (PsAPY1) in pea (Pisum sativum L. var. Alaska), was investigated during early stages of apical hook development using nonradioactive mRNA in-situ hybridization. During the formation of apical hook; at 45 hours after sowing (HAS), expression of PsAPY1 was obvious in epidermis and vascular bundle. By 60 HAS, the apical hook was completely formed. At this stage, transcript accumulation became higher than at the previous stage and expression was also visible in the cortex tissues of the developing hook. However, at 78 HAS, the curvature of the hook was reduced and hook was in the process of opening. At this time, expression of PsAPY1 was visible in all the above-mentioned tissues although the level of expression was slightly lower than at the previous stage (60 HAS). Apical hook formation provides a unique mechanism of protection for delicate shoot meristem in dicot plants. Its establishment is orchestrated by differential elongation rates of cells within the structure. The expression pattern of a gene provides essential information concerning the likely appearance and localization of its encoded protein and this helps to understand the mechanism of development of plant cells and tissues. Higher expression of PsAPY1 during the process of hook development indicates its essential role in the process of formation and maintenance of hook curvature and thus aids in protection of delicate shoot meristem.

scholarly journals Regulation of spatial and temporal gene expression in an animal germline

2018 ◽  
Author(s):  
Asija Diag ◽  
Marcel Schilling ◽  
Filippos Klironomos ◽  
Salah Ayoub ◽  
Nikolaus Rajewsky
Keyword(s):  
Gene Expression ◽  
Regulatory Mechanisms ◽  
Rna Expression ◽  
Temporal Expression ◽  
Temporal Gene Expression ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Gene Regulatory ◽  
Spatio Temporal

SUMMARYIn animal germlines, regulation of cell proliferation and differentiation is particularly important but poorly understood. Here, using a cryo-cut approach, we mapped RNA expression along the Caenorhabditis elegans germline and, using mutants, dissected gene regulatory mechanisms that control spatio-temporal expression. We detected, at near single-cell resolution, > 10,000 mRNAs, > 300 miRNAs and numerous novel miRNAs. Most RNAs were organized in distinct spatial patterns. Germline-specific miRNAs and their targets were co-localized. Moreover, we observed differential 3’ UTR isoform usage for hundreds of mRNAs. In tumorous gld-2 gld-1 mutants, gene expression was strongly perturbed. In particular, differential 3’ UTR usage was significantly impaired. We propose that PIE-1, a transcriptional repressor, functions to maintain spatial gene expression. Our data also suggest that cpsf-4 and fipp-1 control differential 3’ UTR usage for hundreds of genes. Finally, we constructed a “virtual gonad” enabling “virtual in situ hybridizations” and access to all data (https://shiny.mdc-berlin.de/spacegerm/).

scholarly journals BEST: a web server for brain expression Spatio-temporal pattern analysis

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Liyuan Guo ◽  
Wei Lin ◽  
Yidan Zhang ◽  
Wenhan Li ◽  
Jing Wang
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Temporal Pattern ◽  
Pattern Analysis ◽  
Expression Patterns ◽  
Temporal Expression ◽  
Expression Pattern Analysis ◽  
Brain Expression ◽  
Spatio Temporal ◽  
Temporal Expression Pattern

Abstract Background Dysregulated gene expression patterns have been reported in several mental disorders. Limited by the difficulty of obtaining samples, psychiatric molecular mechanism research still relies heavily on clues from genetics studies. By using reference data from brain expression studies, multiple types of comprehensive gene expression pattern analysis have been performed on psychiatric genetic results. These systems-level spatial-temporal expression pattern analyses provided evidence on specific brain regions, developmental stages and molecular pathways that are possibly involved in psychiatric pathophysiology. At present, there is no online tool for such systematic analysis, which hinders the applications of analysis by non-informatics researchers such as experimental biologists and clinical molecular biologists. Results We developed the BEST web server to support Brain Expression Spatio-Temporal pattern analysis. There are three highlighted features of BEST: 1) visualization: it generates user-friendly visual results that are easy to interpret, including heatmaps, Venn diagrams, gene co-expression networks and cluster-based Manhattan gene plots; these results illustrate the complex spatio-temporal expression patterns, including expression quantification and correlation between genes; 2) integration: it provides comprehensive human brain spatio-temporal expression patterns by integrating data from currently available databases; 3) multi-dimensionality: it analyses input genes as both a whole set and several subsets (clusters) which are enriched according to co-expression patterns, and it also presents the correlation between genetic and expression data. Conclusions To the best of our knowledge, BEST is the first data tool to support comprehensive human brain spatial-temporal expression pattern analysis. It helps to bridge disease-related genetic studies and mechanism studies, provides clues for key gene and molecular system identification, and supports the analysis of disease sensitive brain region and age stages. BEST is freely available at http://best.psych.ac.cn.

scholarly journals Functional implications of the utero-placental relaxin (RLN) system in the dog throughout pregnancy and at term

Reproduction ◽  
2017 ◽  
Vol 154 (4) ◽  
pp. 415-431 ◽  
Author(s):  
Marta Nowak ◽  
Aykut Gram ◽  
Alois Boos ◽  
Selim Aslan ◽  
Serhan S Ay ◽  
...  
Keyword(s):  
Domestic Dog ◽  
Dependent Manner ◽  
Temporal Expression ◽  
Functional Roles ◽  
Decidual Cells ◽  
Functional Implications ◽  
Spatio Temporal ◽  
Connective Tissue Remodeling ◽  
Post Implantation

Relaxin (RLN) is a key hormone of pregnancy in mammals best known for its involvement in connective tissue remodeling. In the domestic dog, placental RLN is the only known endocrine marker of pregnancy. However, knowledge is sparse regarding the spatio-temporal expression of RLN and its receptors (RXFP1 and RXFP2) in the canine uterus and placenta. Here, their expression was investigated in the pre-implantation uterus and utero-placental compartments (UtPl) at selected time points during gestation: post-implantation, mid-gestation, and at normal and antigestagen-induced luteolysis/abortion. Immunohistochemistry with newly generated, canine-specific antisera,in situhybridization and semi-quantitative PCR were applied. In compartmentalization studies, placental and endometrialRLNincreased continuously toward prepartum. The placentalRXFP1was time-related and highest during post-implantation and decreased together withRXFP2at prepartum luteolysis. The endometrial levels of both receptors did not vary greatly, but myometrialRXFP2decreased from mid-gestation to prepartum luteolysis. Antigestagen treatment resulted in suppression ofRLNin UtPl and decreasedRXFP1andRXFP2in the uterus. The placental RLN was localized mainly in the cytotrophoblast. Additionally, RXFP1 stained strongly in placental endothelial cells while RXFP2 was found mainly in maternal decidual cells. Uterine staining for all targets was found in epithelial cellular constituents and in myometrium. Finally, besides its endocrine functions, RLN seems to be involved in auto-/paracrine regulation of utero-placental functions in dogs in a time-dependent manner. New insights into feto-maternal communication was provided, in particular regarding the localization of RXFP2 in the maternal decidual cells, implying functional roles of RLN during the decidualization process.

scholarly journals Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development

Life ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 164
Author(s):  
Tanja Šimić Bilandžija ◽  
Katarina Vukojević ◽  
Anka Ćorić ◽  
Ivna Vuković Kekez ◽  
Ivana Medvedec Mikić ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Temporal Expression ◽  
Immunohistochemical Expression ◽  
Ki 67 ◽  
Palate Development ◽  
Significant Difference ◽  
Secondary Palate ◽  
Spatio Temporal ◽  
Apoptotic Activity ◽  
Temporal Expression Pattern

We analyzed the immunohistochemical expression of Ki-67, pRb, Bax, and MMP-9 during the human secondary palate formation (7th to 12th developmental weeks (DWs). The most significant proliferation was observed in the seventh DW with 32% of Ki-67-positive cells in the epithelium, while loose ectomesenchyme condensations (lec) and loose non-condensing ectomesenchyme (lnc) had only 18 and 11%, respectively (Kruskal–Wallis, p < 0.001), and diminished afterwards. Contrarily, pRb-positive cells were mostly located in the lnc (67%), with significant difference in comparison to epithelium and lec in all investigated periods (Kruskal–Wallis, p < 0.001). Ki-67- and pRb-positive cells co-expressed occasionally in all investigated periods. MMP-9 displayed a strong expression pattern with the highest number of positive cells during the seventh DW in the epithelium, with significant difference in comparison to lec and lnc (Kruskal–Wallis, p < 0.0001). The ninth DW is particularly important for the Bax expression, especially in the epithelium (84%), in comparison to lec (58%) and lnc (47%) (Kruskal–Wallis, p < 0.001). The co-expression of Bax and MMP-9 was seen only in the epithelium during seventh and ninth DWs. Our study indicates the parallel persistence of proliferation (Ki-67, pRb) and remodeling (MMP-9) that enables growth and apoptotic activity (Bax) that enable the removal of the epithelial cells at the fusion point during secondary palate formation.

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