Sea anemone (Stichodactyla haddoni) induces apoptosis in lung cancer A549 cells: an in vitro evaluation of biological activity of mucus derivatives

Abstract Small cell lung cancer (SCLC) accounts for 13% ~ 15% of lung cancer. It is a subtype with high malignancy and poor prognosis. Almost all patients with SCLC will inevitably have drug resistance and tumor recurrence, which has become an urgent problem in the treatment of SCLC. Nuclear-targeted drug delivery system, which enables intra-nuclear release of anticancer drugs, is expected to address this challenge. In this study, based on transactivator of transcription (TAT)’s active transport property to the nucleus, we developed a high-efficiency nucleus-targeted co-delivery vector that delivers genes and drugs directly into the nucleus of A549 cells. The system is based on a poly-(N-ε-carbobenzyloxy-L-lysine) (PZLL) and dendritic polyamidoamine (PAMAM) block copolymer (PZLL-D3) with TAT modified on the surface of carrier. In vitro studies showed that DOX and p53 could can be effectively transported to the nucleus and kill the cancer cells. Thus, such deliver system would bypass the drug resistance and tumor recurrence problem.

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Effects of MALAT1 on proliferation and apo- ptosis of human non-small cell lung cancer A549 cells in vitro and tumor xenograft growth in vivo by modulating autophagy

Cancer Biomarkers ◽

10.3233/cbm-170917 ◽

2018 ◽

Vol 22 (1) ◽

pp. 63-72 ◽

Cited By ~ 15

Author(s):

Jun Ma ◽

Kaiming Wu ◽

Kuanzhi Liu ◽

Rong Miao

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

A549 Cells ◽

Small Cell ◽

Tumor Xenograft ◽

Small Cell Lung ◽

Xenograft Growth ◽

Tumor Xenograft Growth

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Baicalein suppresses vasculogenic mimicry through inhibiting RhoA/ROCK expression in lung cancer A549 cell line

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmaa075 ◽

2020 ◽

Vol 52 (9) ◽

pp. 1007-1015

Author(s):

Zhe Zhang ◽

Li Nong ◽

Menglei Chen ◽

Xiaoli Gu ◽

Weiwei Zhao ◽

...

Keyword(s):

Lung Cancer ◽

Vasculogenic Mimicry ◽

A549 Cells ◽

A549 Cell Line ◽

Traditional Herbal Medicine ◽

Underlying Mechanisms ◽

Filament Network ◽

Lung Cancer A549 Cell

Abstract Vasculogenic mimicry (VM) refers to a new tubular network of the blood supply system with abundant extracellular matrix. VM is similar to capillaries but does not involve endothelial cells. As a traditional herbal medicine commonly used in China, baicalein possesses anti-inflammatory and lipoxygenase activities. However, the effects of baicalein on the process of VM formation in non-small cell lung cancer (NSCLC) and the underlying mechanisms have remained poorly understood. In this study, baicalein was found to inhibit the viability and motility of A549 cells and induced the breakage of the cytoskeletal actin filament network. In addition, baicalein significantly decreased the formation of VM and downregulated the expressions of VM-associated factors, such as VE-cadherin, EphA2, MMP14, MMP2, MMP9, PI3K and LAMC2, similar to the effects of ROCK inhibitors. Indeed, baicalein inhibited RhoA/ROCK expression in vitro and in vivo, suggesting the underlying mechanisms of reduced VM formation. Collectively, baicalein suppressed the formation of VM in NSCLC by targeting the RhoA/ROCK signaling pathway, indicating that baicalein might serve as an emerging drug for NSCLC.

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Development and in vitro evaluation of BSA-coated liposomes containing Zn (II) phthalocyanine-containing ferrocene groups for photodynamic therapy of lung cancer

Journal of Organometallic Chemistry ◽

10.1016/j.jorganchem.2020.121469 ◽

2020 ◽

Vol 925 ◽

pp. 121469

Author(s):

Özlem Çoban ◽

Burak Barut ◽

Can Özgür Yalçın ◽

Arzu Özel ◽

Zekeriya Bıyıklıoğlu

Keyword(s):

Lung Cancer ◽

Photodynamic Therapy ◽

In Vitro Evaluation

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Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 CellsIn VitroandIn Vivo

BioMed Research International ◽

10.1155/2016/4567580 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 4

Author(s):

XinGang Lu ◽

Liu Yang ◽

ChengHua Lu ◽

ZhenYu Xu ◽

HongFu Qiu ◽

...

Keyword(s):

Lung Cancer ◽

Mapk Pathway ◽

A549 Cells ◽

Human Lung Cancer ◽

Activity Measurement ◽

Cover Glass ◽

Patchouli Alcohol ◽

Pogostemon Cablin

Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containingPogostemon cablin Benth(also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil ofPogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability bothin vitroandin vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumorin vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.

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Extract from the Leaves of Toona sinensis Roemor Exerts Potent Antiproliferative Effect on Human Lung Cancer Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x02000223 ◽

2002 ◽

Vol 30 (02n03) ◽

pp. 307-314 ◽

Cited By ~ 46

Author(s):

Hui-Chiu Chang ◽

Wen-Chun Hung ◽

Ming-Shyan Huang ◽

Hseng-Kuang Hsu

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Human Lung ◽

Apoptotic Cell ◽

A549 Cells ◽

Lung Cancer Cells ◽

Lung Fibroblasts ◽

Human Lung Cancer ◽

Toona Sinensis

Recent study indicated that the components of Toona sinensis Roemor have potent anti-inflammatory and analgesic effects. These components have also been reported to inhibit the growth of boils in vivo. In this study, we investigated the effect of crude extract from the leaves of Toona sinensis Roemor on the proliferation of A549 lung cancer cells. We found that the extract effectively blocked cell cycle progression by inhibiting the expression of cyclin D1 and E in A549 cells. Additionally, incubation of the extract led to activation of caspase-3-like proteases and apoptotic cell death. Conversely, the extract did not show any significant cytotoxic effect on primarily cultured human foreskin fibroblasts or MRC-5 human lung fibroblasts. Therefore, antiproliferative action of the extract is specific for tumor cells. Our results suggest that the components of Toona sinensis Roemor have potent anticancer effects in vitro and identification of the useful components in the extract may lead to the development of a novel class of anticancer drugs.

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