Antibacterial activity of Mentha piperita L. (peppermint) from leaf extracts - a medicinal plant

The study evaluates the antioxidant, antibacterial and anticancer activities of different extracts of Menthapiperitaleaves against the MCF-7 cell line. The leaves of Menthapiperitawere extracted with aqueous, ethyl acetate, and chloroform. These extracts were subjected to qualitative phytochemical screening, antibacterial activity, cytotoxic activity, and AO/ErBr assay for cells' apoptotic effect against the MCF-7 cell line. Qualitative analysis of the leaves' different extracts revealed glycosides, alkaloids, flavonoids, terpenoids, tannin, and saponin. The antibacterial activity of the leaf extracts was examined against four different bacterial species (Bacillus cereus, Pseudomonasfluorescens, Aeromonas hydrophila, and Klebsiella pneumoniae). The aqueous extract exhibited a high level of antibacterial activity (18.66mm ±1.1 in Bacillus cereus).Free‑radicalscavenging activity of chloroform extractof M. piperita leaves was found to be more than aqueous and ethyl acetate extracts. Further, aqueous, ethyl acetate,and chloroform extracts exerted a cytotoxic effect with the IC50value of 45±1.5μg/ml, 29±1.2μg/ml, and 24±1.0μg/ml, respectively.From this study, we have observed that chloroform extract showed a concentration-dependent apoptotic effect against MCF-7 cellsdetermined by AO/EtBr assay.The resultsfurther depicted that the selected traditionalMentha piperita could be used as a potential anticancer, antibacterial, and good antioxidant agent against the MCF-7 cell line.

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Antibacterial activity of northern Ontario medicinal plant extracts

Canadian Journal of Plant Science ◽

10.4141/cjps2013-258 ◽

2014 ◽

Vol 94 (2) ◽

pp. 417-424 ◽

Cited By ~ 2

Author(s):

Haider M. Hassan ◽

Zi-Hua Jiang ◽

Christina Asmussen ◽

Emma McDonald ◽

Wensheng Qin

Keyword(s):

Antibacterial Activity ◽

Medicinal Plant ◽

Plant Extracts ◽

Micrococcus Luteus ◽

Xanthium Strumarium ◽

Leaf Extracts ◽

Northern Ontario ◽

Cornus Canadensis ◽

Medicinal Plant Extracts

Hassan, H. M., Jiang, Z.-H., Asmussen, C., McDonald, E. and Qin, W. 2014. Antibacterial activity of northern Ontario medicinal plant extracts. Can. J. Plant Sci. 94: 417–424. In the present study, the antibacterial activity (in vitro) of the leaf and/or flower crude extracts of Anaphalis margaritacea (L.) Benth & Hook.f., Grindelia squarrosa (Pursh) Dunal, Apocynum androsaemifolium L., Arctostaphylos uva-ursi (L.) Spreng, Cornus canadensis L. and Xanthium strumarium L. medicinal plants was analyzed through the hole-plate diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays against Escherichia coli, Aeromonas caviae, Paenibacillus alvei, Micrococcus luteus, Mycobacterium avium subsp. avium and Bacillus cereus bacteria. In addition, the time-kill dynamic processes of these extracts against A. caviae, B. cereus, P. alvei, and M. luteus bacteria were also tested. The leaf and flower extracts of Anap. margaritacea and G. squarrosa possess significant antibacterial activity against all the bacteria tested, with inhibition of A. caviae, P. alvei and M. luteus within 1–12 h of incubation at MBC. Particularly, at higher concentrations of the Anap. margaritacea flower crude extract (2–3×MBC), inhibition of A. caviae, B. cereus, P. alvei, and M. luteus bacteria is achieved between 0.5 and 4 h of incubation. In addition, these extracts exhibit high inhibition diameters (majority > 18 mm) and low MIC and/or MBCs (majority=1.25 mg mL−1). In contrast, the leaf extracts of Arct. uva-ursi, X. strumarium, Apoc. androsaemifolium and C. canadensis plants demonstrate moderate to low activity. These results indicate that extracts from Anap. margaritacea and G. squarrosa could be a source of novel antimicrobial scaffolds, compounds or pharmacophores with implications in the pharmaceutical industry and medicine.

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Antibacterial Activity and Phytochemical Analysis of <i>Mentha piperita</i> L. (Peppermint)—An Important Multipurpose Medicinal Plant

American Journal of Plant Sciences ◽

10.4236/ajps.2013.41012 ◽

2013 ◽

Vol 04 (01) ◽

pp. 77-83 ◽

Cited By ~ 22

Author(s):

Para Sujana ◽

Thulasi Muneppa Sridhar ◽

Penchalaneni Josthna ◽

Challagundla Varadarajulu Naidu

Keyword(s):

Antibacterial Activity ◽

Medicinal Plant ◽

Mentha Piperita ◽

Phytochemical Analysis

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Antibacterial activity of Matricaria chamomilla flower and leaf extracts

Planta Medica ◽

10.1055/s-0035-1565653 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

BF Navarro ◽

M del Carmen Ortiz García ◽

BN Marin ◽

ML Rodríguez ◽

AV Hernández

Keyword(s):

Antibacterial Activity ◽

Matricaria Chamomilla ◽

Leaf Extracts

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Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

International Journal of Natural Sciences ◽

10.3329/ijns.v1i4.9733 ◽

1970 ◽

Vol 1 (4) ◽

pp. 82-88 ◽

Cited By ~ 5

Author(s):

MJ Foysal ◽

MM Rahman ◽

M Alam

Keyword(s):

Antibacterial Activity ◽

Pseudomonas Fluorescens ◽

Plant Extracts ◽

Antibiotic Sensitivity ◽

Diffusion Method ◽

Biochemical Tests ◽

Leaf Extracts ◽

Sensitivity Pattern ◽

Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

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Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

BioMed Research International ◽

10.1155/2014/521287 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 14

Author(s):

Somayeh Razmavar ◽

Mahmood Ameen Abdulla ◽

Salmah Binti Ismail ◽

Pouya Hassandarvish

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Phytochemical Screening ◽

Bioactive Constituents ◽

Disc Diffusion Method ◽

Leaf Extracts ◽

Baeckea Frutescens ◽

High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

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