scholarly journals INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA

2017 ◽  
Vol 4 (1) ◽  
pp. 41
Author(s):  
Ni Luh Putu Kayika Febryanti ◽  
Made Ria Defiani ◽  
Ida Ayu Astarini
Keyword(s):  
Sweet Corn ◽  
Shoot Multiplication ◽  
Basic Medium ◽  
Shoot Height ◽  
Randomized Design ◽  
In Vitro Technique ◽  
Number Of Leaves ◽  
Range Test ◽  
Mathematics And Science

Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.

scholarly journals PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

2016 ◽  
Vol 31 (1) ◽  
pp. 33
Author(s):  
Sri Hartati ◽  
Agus Budiyono ◽  
Ongko Cahyono
Keyword(s):  
Plant Conservation ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Significant Difference ◽  
Two Factors ◽  
Number Of Leaves ◽  
Media Research ◽  
Test Level ◽  
Range Test

<p><em>Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  </em><em>hybrids</em><em> orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. </em><em>The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of </em><em>Benzyl Amino Purine</em><em> /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). </em><em>Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that </em><em>the addition  </em><em>of NAA 3 ppm </em><em>as much as 4,96 cm </em><em> and BAP 3 ppm </em><em>as much as 4,41 cm </em><em> give a significant effect on the increase of high plantlets</em><em> </em><em>and  so NAA 3 ppm </em><em>as much as 5,76 cm </em><em> effect  on  roots length , but did not significantly effect the number of leaves and roots.</em></p>

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

2019 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani
Keyword(s):  
Musa Acuminata ◽  
Laboratory Research ◽  
Independent Effect ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

scholarly journals Multiplikasi Tunas dan Induksi Perakaran Pada Perbanyakan Rhododendron radians J.J.Sm (Ericaceae) Secara In Vitro [Shoot Multiplication and Root Induction on In Vitro Propagation of Rhododendron radians J.J.Sm (Ericaceae)]

2019 ◽  
Vol 28 (1) ◽  
pp. 51
Author(s):  
Tri Warseno ◽  
Dyan Meiningsasi Siswoyo Putri
Keyword(s):  
Growth Regulators ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Root Induction ◽  
Plant Materials ◽  
Shoot Height ◽  
Randomized Design ◽  
Starting Point ◽  
Four Levels

<p><em>Rhododendron</em> radians merupakan tanaman hias yang hanya ditemukan di Sulawesi Tengah dan Utara. Perbanyakan secara massal diperlukan untuk komersialisasi tanaman tersebut. Penelitian bertujuan mendapatkan teknik mikropropagasi secara in vitro yang tepat untuk R. radians melalui percobaan pemberian zat pengatur tumbuh (ZPT) pada media kultur. Penelitian terdiri atas dua tahap percobaan. Percobaan 1 perlakuan kombinasi ZPT untuk proliferasi tunas disusun menggunakan rancangan acak lengkap dengan satu faktor, yaitu media (delapan kombinasi media) dengan menggunakan dua konsentrasi IAA (0,1 mg/l dan 1,0 mg/l) dan empat konsentrasi 2iP (0, 6, 7, dan 8 mg/l). Percobaan 2 adalah perlakuan IBA untuk pertumbuhan tunas dan pengakaran menggunakan lima variasi media (M1 = media WPM (kontrol), M2 = media WPM + 0,25 mg/l IBA, M3 = media WPM + 0,5 mg/l IBA, M4 = media WPM + 1 mg/l IBA, M5 = media WPM + 5 mg/l IBA). Hasil penelitian menunjukan bahwa media WPM dengan penambahan 1,0 mg/l IAA dan 7,0 mg/l 2iP merupakan media terbaik untuk induksi tunas pada R. radians, dengan jumlah tunas rata-rata 15,80 + 3,45 cm dan tinggi tunas rata-rata 2,36 + 0,25 cm, sedangkan media terbaik untuk induksi akar adalah media M4 (WPM + 1,0 mg/l IBA), dengan persentase eksplan berakar 63,33% dan panjang akar rata-rata 4,7 mm. Hasil penelitian diharapkan dapat digunakan sebagai acuan awal untuk melakukan perbanyakan secara massal R. radians dan diharapkan penelitian lebih lanjut dapat dilakukan sampai dengan tahap aklimatisasi.</p><p><strong>Keywords</strong></p><p>Auksin; Media; Mikropropagasi; <em>Rhododendron radians</em></p><p><strong>Abstract</strong></p><p>Rhododendron radians is an ornamental plant that found only in Sulawesi (Central and North). Commercialization of this species requires mass propagated plant materials. This study aimed to determine the proper technique for R. radians micropropagation in vitro. This study used two stages of treatment i.e (1) treatment of IAA on shoot proliferation R. radians arranged in completely randomized design with one factor (eight medium combination), the concentration of plant growth regulators IAA which consisted of two levels: 0.1 mg/l, and 1 mg/l and concentration of growth regulators 2iP consisting of four levels (0,6, 7, and 8 mg / l); (2) IBA treatment on shoot growth and rooting using five variations of the medium (M1 = WPM (control), M2 = WPM + 0.25 mg/l IBA, M3 = WPM + 0.5 mg/l IBA, M4 = WPM + 1 mg/l IBA; M5 = WPM + 5 mg/l IBA). The results showed that the WPM added with 1 mg/l IAA and 7 mg/l 2iP was the best medium for shoot induction initiated from seed culture, with the average number of shoots 15. 80 ± 3.45 cm and an average shoot height of 2.36 ± 0.25 cm. While the best medium for root induction was M4 (WPM + 1 mg / l IBA), with a percentage of 63.33% rooted explants and the average root length of 4.7 mm. The results of this study can be used as a starting point to conduct mass propagation R. radians.</p>

scholarly journals The Effect of Benzyl Amino Purine (BAP) Concentration on the Growth Amount of the Explant of Dendrobium spectabile Orchid by In-Vitro

2020 ◽  
Vol 3 (2) ◽  
pp. 33
Author(s):  
Ario Ario ◽  
Setiawan Setiawan
Keyword(s):  
Economic Value ◽  
Aloe Vera ◽  
Leaf Length ◽  
Plant Seeds ◽  
Randomized Design ◽  
In Vitro Technique ◽  
Number Of Leaves ◽  
Short Time ◽  
Benzyl Amino Purine

<em>Dendrobium</em><em> spectabile orchid is an ornamental plant of high economic value, having relatively durable properties, beauty, and attractiveness of orchids lie in the diverse shapes and colors of the flowers. Therefore, it requires rapid and efficient propagation techniques. One of them is in-vitro vegetative propagation. Plant propagation using the in-vitro technique has the opportunity to produce a large number of plant seeds in a relatively short time. This study aims to determine the correct concentration of Benzyl Amino Purine (BAP) on the growth amount of the explants of Dendrobium spectabile orchid by in-vitro. The research was conducted at the Biotechnology Laboratory of the Regional Technical Implementation Unit (UPTD) of the Aloe Vera Center (AVC) Pontianak. This research was conducted from January 1, 2020, to March 30, 2020. The method used was a Completely Randomized Design (CRD). The treatment consisted of 6 levels of treatment, namely b1 = 0.75 ppm, b2 = 1.00 ppm, b3 = 1.25 ppm, b4 = 1.50 ppm, b5 = 1.75 ppm, b6 = 2.00 ppm. All treatments were repeated 4 times for each treatment consisting of 3 plant samples. The research finding showed that the treatment of BAP concentration 1.50 was the best in increasing the average number of leaves 4.92 strands, the average number of shoots 4.25 buds, and the average leaf length was 2.44 cm.</em>

scholarly journals MULTIPLIKASI TUNAS PUCUK TOMAT (Lycopersicum esculentum MILL) DENGAN MENGGUNAKAN BENZYL AMINO PURINE (BAP) DAN NAPHTALENE ACETIC ACID (NAA) SECARA IN VITRO

2019 ◽  
Author(s):  
F. Fathurrahman
Keyword(s):  
Acetic Acid ◽  
Shoot Multiplication ◽  
Shoot Height ◽  
Factor B ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

Research with the title shoot multiplication shoots tomatoes (Lycopersicumesculentum mill) using the Benzyl Amino Purine (BAP) dan Naphtalene Acetic Acid(NAA) in vitro have been conducted at the Laboratory of Biotechnology Faculty ofAgriculture, Islamic University of Riau, Pekanbaru. This research has been carried outfor three months carried out startingfrom November 2010 to February 2011. This studyaims to determine the effect of a single interaction between the administration and plantgrowth regulators BAP and NAA on shoot multiplication of in tomato shoots vitro.Rancangan used in this study was completely randomized design (CRD) in factorialwhich consist of two factors. The first factor is factor B (concentration of BAP) withfour standard treatments are: B0 (0 ppm), B1 (1 ppm), B2 (2 ppm), B3 (3 ppm).Thesecond factor is the factor (the concentration of NAA) with four standard treatments,including: N0 (0 ppm), N1 (0.1 ppm), N2 (0.5 ppm), and N3 (1 ppm), to obtain 16combined treatment with three replications. Parameters observed, namely: age emergedshoots, number of shoots, shoot height, the percentage of growing shoots, roots andgrowing percentage of the number of explants forming callus. The data was statisticallyanalyzed the results of observations, when the F calculated is greater than the F table,followed by a further test of honest real difference (HRD) 5%. From the results ofresearch in the interaction of BAP and NAA effect on the parameters of high-shoots bytreatment tebaik B1N0 namely 6.16 cm. BAP singly significantly affect the parametersage appears buds (days) and the percentage grows shoots with the best treatment B2 (2ppm), shoot height with the best treatment B0 (0 ppm), the number of shoots (the fruit)with the best treatment B3 (3 ppm). singly whereas NAA significantly affect theparameters age appears shoots (day) and high-shoots with the best treatment N0 (0ppm), and the percentage grows roots with the best treatment N3 (1 ppm).

scholarly journals MULTIPLIKASI TUNAS DAN AKLIMATISASI PEGAGAN (Centella asiatica L.) PERIODE KULTUR LIMA TAHUN

2020 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
NATALINI NOVA KRISTINA ◽  
DEDI SURACHMAN
Keyword(s):  
Rice Husk ◽  
Research Result ◽  
Shoot Multiplication ◽  
Centella Asiatica ◽  
Randomized Design ◽  
Medicinal Crop ◽  
Pedicle Length ◽  
Number Of Leaves ◽  
Completely Randomized Design

ABSTRAK<br />Pegagan (Centella asiatica L.) adalah tanaman obat yang<br />mengandung zat asiaticotik sebagai obat alzaimer dan penghalus kulit.<br />Tanaman ini telah diperbanyak sejak tahun 2000. Penelitian dilakukan di<br />Laboratorium Kultur Jaringan dan Rumah Kaca Kelompok Peneliti<br />Plasma Nutfah dan Pemuliaan, Balai Penelitian Tanaman Obat dan<br />Aromatik, Bogor dari bulan Januari 2000 sampai dengan Juni 2005.<br />Penelitian ini bertujuan untuk melihat daya multiplikasi tunas setiap<br />periode subkultur dimulai dari tahun kedua sampai periode lima tahun.<br />Media yang digunakan adalah MS + BA 0,1 mg/l. Pengamatan dilakukan<br />pada jumlah tunas, jumlah daun dan visual tunas pada umur 2, 3, 4 dan 5<br />tahun pada dua periode subkultur setiap tahunnya. Penelitian disusun<br />dengan rancangan acak lengkap, masing-masing terdiri atas 10 botol yang<br />merupakan ulangan dan setiap botol terdiri atas 1 eksplan. Untuk re-media<br />terhadap tanaman yang terlihat berubah digunakan media MS + BA (0;<br />0,1; 0,2; 0,3) mg/l. Selanjutnya untuk perakaran dilakukan pada media MS<br />+ IAA (0,1 dan 0,2); MS + NAA (0,1 dan 0,2) mg/l serta MS + IBA (0,1<br />dan 0,2) mg/l. Plantlet utuh yang terbentuk selanjutnya diaklimatisasi pada<br />media tanah + pupuk kandang dan tanah + sekam dengan perbandingan 1 :<br />1. Hasil penelitian menunjukkan bahwa daya multiplikasi tunas optimum<br />terjadi pada tahun ketiga, dan memasuki tahun keempat dan kelima<br />menurun yang diiringi dengan perubahan eksplan yang terlihat pada<br />tangkai daun yang terbentuk. Akar terpanjang dan terbanyak yang<br />terbentuk didapat pada media IAA 0,2 mg/l dengan penampilan yang<br />kurus dan rapuh. Keberhasilan aklimatisasi sangat rendah, tetapi plantlet<br />mampu beregenerasi dengan baik dan terlihat tumbuh normal. Dari hasil<br />perbanyakan terlihat bahwa jumlah anakan, jumlah daun, panjang stolon<br />dan jumlah bunga lebih tinggi dibandingkan yang tumbuh pada media<br />sekam, berturut-turut : 6,77; 7,30; 46,50 cm dan 8,31. Sementara pada<br />media sekam komponen yang dominan adalah panjang tangkai daun yakni<br />9,75 cm.<br />Kata kunci : Pegagan, Centella asiatica L., multiplikasi, tunas, aklima-<br />tisasi, penyimpanan, Jawa Barat<br />ABSRACT<br />Shoot multiplication and acclimatization of gotuloca<br />(Centella asiatica L.) five years after conservation by in<br />vitro culture<br />Gotuloca (Centella asiatica L.) is a medicinal crop containing<br />asiaticotic as alzaimer and skin revitalizer. This crop has been<br />multiplicated in vitro since 2000. This research was carried out in the<br />laboratory and glasshouse of Breeding and Germplasm Group in the<br />Indonesian Medicinal and Aromatic Crops Research Institute (IMACRI)<br />from January 2000 to June 2005. The objective of the research was to find<br />out the viability of shoots multiplication after two year to five year period,<br />with media MS + BA 0.1 mg/l. The parameters observed were the number<br />of shoots, the number of leaves at the age 2, 3, 4 and 5 years from two<br />subculture periods every year. The treatments were arranged in a<br />completely randomized design, each replication consisted of 10 bottles and<br />each bottle consisted of 1 explant. After subculture the ex-plant were re-<br />media in medium MS + BA (0; 0,1; 0,2; 0,3) mg/l. The rooting media<br />before glasshouse were : MS + IAA ( 0,1 and 0,2); MS + NAA ( 0,1 and<br />0,2) mg/l; and MS + IBA ( 0,1 and 0,2) mg/l. The plantlets formed were<br />acclimatized using soil + cattle manure and soil + rice husk with<br />comparison 1:1. Research result indicated that the optimum viability<br />multiplication was achieved in the third year, and it decreased after the<br />fourth and fifth years with change in explant forming the petiole. The<br />longest and plantlet roots were formed through media IAA 0.2 mg/l with<br />brittle and thin appearance, but the plantlets were able to regenerate better<br />and grow normal. The acclimatization was not very successful but the<br />plantlets could regenerate and grew normally. The multiplication showed<br />that the number of stumps, leaves, stolons and flowers were : 6,77; 7,30;<br />46,50 cm and 8,31 respectively. In rice husk media the dominant<br />component was pedicle length 9,75 cm.<br />Key words : Gotuloca, Centella asiatica L., multiplication, shoot,<br />acclimatization, conservation, East Jav

scholarly journals PENGARUH BEBERAPA JENIS SITOKININ PADA MULTIPLIKASI TUNAS ANGGREK Vanda douglas SECARA IN VITRO

2017 ◽  
Vol 4 (1) ◽  
pp. 36 ◽  
Author(s):  
Karyanti Karyanti
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Design Experiment ◽  
Randomized Design ◽  
Leaf Formation ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Positive Effect ◽  
Number Of Shoots

The Effect of Several Types of Cytokinin on Shoot Multiplication of Vanda douglas Orchid In VitroThe study was aimed to determine the response of Vanda douglas orchid on shoot-multiplication media to different cytokinin concentrations in vitro. A completely randomized design experiment was employed with one factor cytokinin, in which the cytokinins used were TDZ (thidiazuron), BAP (6-Benzylaminopurine) and kinetin at the concentrations of 0, 0.5, 1, and 1.5 mg/L. The results showed that kinetin 0.5 mg/L was the best concentration for shoot formation, occuring on average at 14.88 days after planting; while TDZ 0.5 mg/L was the best concentration for increasing the height of the plant, being on average 0.53 cm. TDZ at 0.5 mg/L concentration also had positive effect on shoot and leaf formation, which resulted in the highest number of shoots and leaves. The average number of shoots was 8.00 buds, and the average number of leaves was 12.25 sheets. Keywords: Vanda douglas, thidiazuron, BAP, kinetin, shoots multiplication ABSTRAKPenelitian ini bertujuan untuk mengetahui respon anggrek Vanda douglas terhadap media perbanyakan tunas pada beberapa konsentrasi jenis sitokinin secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor, yaitu sitokinin. Sitokinin yang digunakan adalah TDZ (thidiazuron), BAP (6-Benzylaminopurine) dan kinetin, dengan konsentrasi 0, 0,5, 1, dan 1,5 mg/L. Hasil penelitian menunjukkan bahwa pemberian kinetin 0,5 mg/L menunjukkan hasil terbaik pada peubah waktu pembentukan tunas, dengan rata-rata 14,88 hari setelah tanam. Sedangkan konsentrasi TDZ 0,5 mg/L merupakan jenis sitokinin dan konsentrasi terbaik terhadap pertambahan tinggi tanaman, dengan rata-rata 0,53 cm. TDZ dengan konsentrasi 0,5 mg/L juga berpengaruh positif terhadap pertumbuhan tunas dan daun, dengan menghasilkan jumlah tunas tertinggi dan jumlah daun terbanyak. Rata-rata jumlah tunas adalah 8,00 tunas, dan rata-rata jumlah daun adalah 12,25 helai.Kata Kunci: Vanda douglas, thidiazuron, BAP, kinetin, perbanyakan tunasReceived: 19 June 2017        Accepted: 02 July 2017        Published: 12 July 2017

scholarly journals The effect of coconut water and benzyl amino purine (BAP) addition to the growth of pineapple from Sipahutar North Sumatera in vitro condition

2018 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
Harifah Insani ◽  
Fauziyah Harahap ◽  
Diky Setya Diningrat
Keyword(s):  
Tissue Culture ◽  
Leaf Length ◽  
Coconut Water ◽  
Basic Medium ◽  
Shoot Height ◽  
Culture Techniques ◽  
Base Medium ◽  
Randomized Design ◽  
Benzyl Amino Purine

Background: Pineapple fruit used in this study was obtained from Sipahutar sub-district, North Tapanuli regency, North Sumatra which was a superior commodity fruit. Propagation of the Sipahutar pineapple plant was conducted vegetatively using bud stems and crown shoots, but the amount was limited to be planted in large areas. Therefore, propagation through tissue culture techniques could be used as an alternative solution.Objective: Explants source were taken from plantlets in vitro aged 3 months were grown on Murashige and Skoog (MS) basic medium with the addition of Indole Acetic Acid (IAA) 1 ppm.Methods: Sipahutar pineapple tissue culture work has been done in YAHDI Plant Tissue Culture Laboratory, Medan. The study design used a factorial completely randomized design. The first factor is coconut water with concentration of 0%, 10%, 20%, and 30%, second factor is Benzyl Amino Purine (BAP) with concentration 0 ppm, 2 ppm and 4 ppm, so there were 12 combination with 3 repetition.Result: The results showed that the addition of coconut water on MS base medium with IAA of 1 ppm gave a significant effect on the number of leaf yield and shoot height. The provision of BAP on basic MS medium with IAA of 1 ppm gave a significant effect on the number of shoots produced, the number of leaves produced, the length of the leaves, the width ofleaves and the length of shoots. The interaction of coconut water and BAP addition on MS base medium with IAA of 1 ppm gave significant effect on leaf length, leaf width and shoot length as well.Conclusions: MS + IAA basic media combined with coconut water + BAP could stimulate the growth of Sipahutar pineapple in vitro.  

scholarly journals The shoot explant types effect of eleven stevia (Stevia rebaudiana Bertoni)

Jurnal Agro ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 142-152
Author(s):  
Suseno Amien ◽  
Arini Zahra Azhari ◽  
Citra Bakti ◽  
Haris Maulana
Keyword(s):  
Stevia Rebaudiana ◽  
Shoot Tip ◽  
Shoot Height ◽  
Stevia Rebaudiana Bertoni ◽  
Randomized Design ◽  
Two Factors ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Number Of Shoots

Explants play an important role in thepropagation system of stevia (Stevia rebaudiana Bertoni). A Completely Randomized Design (CRD) was used in this experiment with factorial pattern consisting of two factors, namely three types of explants (shoot tip, first node, and second node) and eleven accessions of stevia namely a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). The results showed that shoot tip explant was the best explant than first node and second node for the number of shoots (18.11 shoots), number of leaves (93.49 leaves) and wet weight (3.56 grams). The best accession of shoot height was a10(SGR 7.5) (19.95 cm), the highest number of shoots wasa7(SBG 10) (21.87 shoots), the highest number of leaves wasa7(SBG 10) (138.00 leaves), the heaviest wet weight wasa7(SBG 10) (3.56 grams), the highest leaf chlorophyll content was a10 (SGR 7.5) (0.63 µg mL-1). Accessions with the fastest root initiation time at the rooting stage was a11 (TR 3.5) (4.00 DAC), the highest number of roots wasa10 (TR 3.5) (27.11 roots), the best root length wasa2(Garut) (4.51 cm). Information on the best explant types and stevia accessions in the in-vitro multiplication stage can be used as the basis for stevia breeding programs in Indonesia.AbstractEksplan berperan penting dalam sistem perbanyakan Stevia (Stevia rebaudiana Bertoni). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu tiga jenis eksplan (ujung pucuk, ruas pertama, dan ruas kedua) dan sebelas aksesi stevia yaitu a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). Hasil penelitian menunjukkan bahwa eksplan ujung pucuk merupakan eksplan terbaik dibandingkan buku pertama dan kedua untuk jumlah tunas (18,11 tunas), jumlah daun (93,49 daun) dan berat basah (3,56 gram). Aksesi terbaik pada tinggi pucuk adalah a10(SGR 7.5) (19,95 cm), jumlah pucuk tertinggi a7 (SBG 10) (21,87 pucuk), jumlah daun terbanyak a7 (SBG 10) (138.00 helai daun), bobot basah terberat a7 (SBG 10) (3,56 gram), kandungan klorofil daun tertinggi adalah GR 7,5 (0,63 µg mL-1). Aksesi dengan waktu inisiasi akar tercepat pada tahap perakaran adalah TR 3,5 (4,00 HST), jumlah akar terbanyak adalah TR 3,5 (27,11 akar), panjang akar terbaik adalah a2(Garut) (4,51 cm). Informasi jenis eksplan dan aksesi stevia terbaik pada tahap multiplikasi in-vitro dapat dijadikan dasar program pemuliaan stevia di Indonesia.

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