scholarly journals Development and Validation of a Simple RP-HPLC-PDA Method for Determination of 18 Polyphenols in Grape Juice and Red Wine

2021 ◽  
Vol 9 (8) ◽  
pp. 1599-1605
Author(s):  
Nilüfer Vural ◽  
Özlem Yalçınçıray
Keyword(s):  
Grape Juice ◽  
Average Concentration ◽  
Red Wines ◽  
Rp Hplc ◽  
Relative Standard ◽  
Water Ph ◽  
Grape Juices ◽  
Development And Validation ◽  
Gradient Phase

According to the trend of a healthy eating awareness trend, having a potential benefit on human health, some polyphenols like flavonoids, resveratrol, hydroxy-stilbenes, and phenolic acids are in the spotlight. Grapes, and one of the most widespread grape product wine; are among the best sources of these polyphenols. In this study, a highly specific, susceptible, and easy chromatographic method was brought out and validated to determine 18 polyphenols in grape and red wines. For this aim, an HPLC-PDA was used, and the separation was accomplished on an RP-ODS4 column. The method comprised of a mobile gradient phase consisting of A solution (acetic acid in water, pH 2.00) and a mixture of the solution A – acetonitrile (20:80, v/v), at a flow rate of 1.0 ml/min, and PDA detection was carried out at 260,280, 320, and 360 nm. According to the results, it can be said that the program indicated good linearity over the range of 1-40 mg L−1 of phenolics with r2>0.99. The recovery of the 18 phenolics ranges from 83.17% to 119.88% at red wines and 88.20% to 117.83% at grape juices. The method is well precise, with the relative standard deviation (RSD) of the average concentration of the phenolic compounds are ranges from 1.22% to 2.02% at red wines and 1.01% to 2.56% at grape juices.

DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF BERBERINE AND CURCUMIN IN AN AYURVEDIC FORMULATION

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (08) ◽  
pp. 48-52
Author(s):  
K. P Parekh ◽  
◽  
A. P. Jadhav
Keyword(s):  
Mobile Phase ◽  
Herbal Medicines ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Quality And Safety ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Water Ph ◽  
Development And Validation

A simple, accurate, precise, robust stability indicating RP-HPLC method was developed and validated for simultaneous estimation of berberine and curcumin in an ayurvedic formulation. The two markers were resolved using a C-18 column using as the mobile phase methanol: water (pH 3 adjusted using acetic acid) in the ratio 75:25 V/V at a flow rate of 1mL/min. Retention times of berberine and curcumin were 2.58 ± 0.2 min and 8.5 ± 0.2 min, respectively at 358 nm. Linear response was observed in the concentration range of 2 – 8 ppm for berberine and 5 – 40 ppm for curcumin, with correlation coefficient (r2) of 0.994 and 0.998 for berberine and curcumin, respectively. The developed method was applied for quantitation of markers in marketed and in-house formulations of Gruhadhoomadi Churna. This method can also be used to evaluate formulations containing berberine and curcumin as markers, thus conforming to the need of ensuring quality and safety of herbal medicines.

Development and Validation of an RP-HPLC Method for Determination of Atorvastatin and its Hydroxyl Metabolites in Human Plasma

2020 ◽  
Vol 16 (3) ◽  
pp. 238-245
Author(s):  
Dagmara Sowińska ◽  
Alicja Pogorzelska ◽  
Marlena Rakicka ◽  
Justyna Sznura ◽  
Justyna Janowska ◽  
...  
Keyword(s):  
Human Plasma ◽  
Internal Standard ◽  
Hplc Method ◽  
Active Metabolites ◽  
Peak Separation ◽  
Rp Hplc ◽  
Increased Risk ◽  
Relative Standard ◽  
Development And Validation

Background: Atorvastatin (AT) belongs to cholesterol-lowering agents, commonly used in patients with an increased risk of cardiovascular disease. The drug, as well as its hydroxyl metabolites, exhibit pharmacological activity, and their plasma levels may be helpful in the assessment of the therapeutic effectiveness. Objective: Development and validation of a fast and reproducible RP-HPLC method with UV detection for the simultaneous determination of atorvastatin and its active metabolites, para-hydroxy-atorvastatin (p-OH-AT) and ortho-hydroxy-atorvastatin (o-OH-AT) in human plasma. Methods: Optimal conditions of chromatographic separation of the analytes, as well as rosuvastatin, chosen as an internal standard, were studied. The absorbance of the compounds was measured at λ=248 nm. Validation of the method was performed. The usefulness of the method was confirmed for determination of the analytes in plasma of patients treated with the drug. Results: Total peak separation was achieved at LiChrospher 100 RP-18 column with a mobile phase composed of methanol and water (1:1,v:v) and a flow rate of 1.2 ml/min. The method was linear in the ranges of 0.025 - 1.0 μg/ml for AT, o-OH-AT and p-OH-AT. Intra- and inter-assay precision expressed as relative standard deviation was ≤13% for AT, ≤12% for p-OH-AT and ≤11% for o-OH-AT. Intraand inter-day accuracy of the method, expressed as a relative error, was ≤15%. Conclusion: The elaborated HPLC method is specific, repeatable, reproducible, adequately accurate and precise and fulfills the validation requirements for the bioanalytical method. The method was successfully applied for analysis of atorvastatin and its o-hydroxy metabolite in plasma of patients treated with the drug.

DEVELOPMENT AND VALIDATION OF DETERMINATION FOR CHLORPHENIRAMINE MALEATE, PHENYLEPHRINE HYDROCHLORIDE AND IBUPROFEN IN BULK AND PHARMACEUTICAL FORMULATION BY RP-HPLC METHOD

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (11) ◽  
pp. 35-40
Author(s):  
V. B. Baviskar ◽  
◽  
S. T. Donda ◽  
S. S. Patil ◽  
P. K Deshmukh ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Orthophosphoric Acid ◽  
Pharmaceutical Preparation ◽  
Hplc Method ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Rp Hplc ◽  
Water Ph ◽  
Development And Validation

A simple, precise and accurate isocratic RP-HPLC method was successfully developed for the simultaneous determination of chlorpheniramine maleate (CPM), phenylephrine hydrochloride (PHE) and ibuprofen (IBU) in bulk and pharmaceutical preparation. The separation of the drugs was achieved using Inertsil ODS C18 column with mobile phase consisting of methanol and 0.1% triethylamine (55:45 V/V) in water, pH 3 (adjusted with orthophosphoric acid). These drugs were resolved successfully with retention time of 3.0 min for CPM, 4.6 min for PHE and 18.9 min for IBU, when the eluate was monitored at 240 nm. Linearity was found in the concentration range of 10 to 50 μg mL-1 for CPM, 25 to 125 μg mL-1 for PHE and 100 to 500 μg mL-1 for IBU, with a correlation coefficient (r2) of 0.9987, 0.9965 and 0.9998 for CPM, PHE and IBU, respectively. Thus, the proposed method can be successfully applied to the pharmaceutical preparation containing the above mentioned drugs without any interference of excipients.

scholarly journals Development and Validation of a New RP-HPLC Analytical Method for the Simultaneous Determination of Luliconazole and Clobetasol Propionate in Synthetic Mixture

2021 ◽  
pp. 53-60
Author(s):  
Binal Solanki ◽  
Hirak Joshi
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Clobetasol Propionate ◽  
Synthetic Mixture ◽  
Reverse Phase ◽  
Injection Volume ◽  
Rp Hplc ◽  
Water Ph ◽  
Development And Validation

Aim: To develop new selective and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) approach for the quantification of antifungal drug Luliconazole integrate with corticosteroid drug Clobetasol Propionate in a synthetic mixture. Methods: The method was validated to achieve International Conference Harmonization (ICH) requirements. Chromatographic separation was carried out by isocratic technique on a reversed-phase Inertsil C18 column (5 µm, 250mm x 4.6mm i.d with the mixture of Acetonitrile: Water pH adjusted with H3PO4 (60: 40) and UV detection at 264 nm. The compounds were eluted at a flow rate of 1.0 mL/min with an injection volume of 20μL. Results: The calibration curves were linear (r2 > 0.999) over the concentration range 10-200 μg/mLfor Luliconazole and 5-100 μg/mL for Clobetasol Propionate. The average retention times for Luliconazole and Clobetasol Propionate were 3.16 and 6.94 min, respectively. The % RSD   for the proposed method was found to be less than %2. The % recovery was found to between 99.22-99.48% for the developed method. Conclusion: The developed method is simple, rapid, precise, and accurate and hence was successfully applied for the determination of Luliconazole and Clobetasol Propionate in a synthetic mixture.

scholarly journals DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID, CURCUMIN AND PIPERINE IN AN AYURVEDIC FORMULATION

2020 ◽  
pp. 81-86
Author(s):  
MEHJABEEN SHAIKH ◽  
ARUNA P. JADHAV
Keyword(s):  
Gallic Acid ◽  
Mobile Phase ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Robust Method ◽  
Isocratic Elution ◽  
Rp Hplc ◽  
Relative Standard ◽  
Water Ph ◽  
Development And Validation

Objective: The objective of the present work was to establish a simple, precise, accurate and robust method for simultaneous estimation of gallic acid, curcumin and piperine from the marketed ayurvedic formulation by liquid chromatography. Methods: The separation was carried out on Hemochrom C18 Column (250 mm × 4.6 mm ID, 5 µm pore size) with a mobile phase methanol: acetonitrile: water (pH 3.2adjusted by using orthophosphate acid) in the ratio 70:20:10v/v by isocratic elution modeat 25 °C and the flow rate was setat0.8 ml/min. The analysis was carried out atisoabsorptive wavelength of 295 nm. Results: The retention time of gallic acid, curcumin and piperine was found to be 3.3(±0.2), 4.7 (±0.2) and 5.6 (±0.2) min, respectively. The linearity range for gallic acid, curcumin and piperine was found to be 10-70 μg/ml, 20-80 µg/ml and 2-14 µg/ml, respectively with the coefficient of linear regression greater than 0.99 for all markers. Mean percent recoveries for gallic acid, curcumin, and piperine were found within the limit of acceptance (99-100%). The percent relative standard deviation (%RSD) for precision and robustness was found less than 2%, which indicates the method is precise and robust. The developed method applied for quantification of these markers from the marketed ayurvedic formulation of Dekofcyn tablet. Conclusion: The developed method was found to be simple, rapid, precise and reproducible for standardization of Dekofcyn tablet and can be useful for other formulations containing these three markers.

scholarly journals A novel LC-MS method development and validation for the determination of phenyl vinyl sulfone in eletriptan hydrobromide

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Indhu Priya Mabbu ◽  
G. Sumathi ◽  
N. Devanna
Keyword(s):  
Method Development ◽  
Limit Of Detection ◽  
Vinyl Sulfone ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical ◽  
Development And Validation ◽  
Phenyl Vinyl

Abstract Background The aim of the present method is to develop and validate a specific, sensitive, precise, and accurate liquid chromatography-mass spectrometry (LC-MS) method for the estimation of the phenyl vinyl sulfone in the eletriptan hydrobromide. The effective separation of the phenyl vinyl sulfone was achieved by the Symmetry C18 (50 × 4.6 mm, 3.5 μm) column and a mobile phase composition of 0.1%v/v ammonia buffer to methanol (5:95 v/v), using 0.45 ml/min flow rate and 20 μl of injection volume, with methanol used as diluent. The phenyl vinyl sulfone was monitored on atomic pressure chemical ionization mode mass spectrometer with positive polarity mode. Results The retention time of phenyl vinyl sulfone was found at 2.13 min. The limit of detection (LOD) and limit of quantification (LOQ) were observed at 1.43 ppm and 4.77 ppm concentration respectively; the linear range was found in the concentration ranges from 4.77 to 27.00 ppm with regression coefficient of 0.9990 and accuracy in the range of 97.50–102.10%. The percentage relative standard deviation (% RSD) for six replicates said to be injections were less than 10%. Conclusion The proposed method was validated successfully as per ICH guidelines. Hence, this is employed for the determination of phenyl vinyl sulfone in the eletriptan hydrobromide.

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