ISOLASI BAKTERI AMILOLITIK DARI RAGI

Jefri Nur Hidayat; Dyah Ayu Fajarianingtyas

doi:10.24929/lensa.v3i2.1260

ISOLASI BAKTERI AMILOLITIK DARI RAGI

LENSA (Lentera Sains) Jurnal Pendidikan IPA ◽

10.24929/lensa.v3i2.1260 ◽

2021 ◽

Vol 3 (2) ◽

pp. 39-42

Author(s):

Jefri Nur Hidayat ◽

Dyah Ayu Fajarianingtyas

Keyword(s):

Soluble Starch ◽

Starch Agar

Penelitian ini bertujuan untuk mendapatkan bakteri amilolitik dan untuk mengetahui aktifitas amilase. Uji aktifitas kualitatif amilase menggunakan media SSA. Hasil isolasi bakteri yang diisolasi dari ragi tape berjumlah 7 isolat. Kemudian 7 isolat bakteri diberi kode: a, b, c, d, e, f dan j. Tujuh isolat dilakukan seleksi astau skrining dengan menumbuhkan pada medium Soluble Starch Agar (SSA). Hasil seleksi diperoleh 4 isolat yang menunjukkan adanya aktifitas amilase ektraseluler. Aktifitas enzim tertinggi terdapat pada isolat j yang memiliki indeks zona bening koloni sebesar 12. Setiap kinerja enzim dipengaruhi oleh keadaan lingkungan seperti pH dan suhu. Enzim mempunyai konstanta disosiasi pada gugus asam maupun gugus basanya, terutama pada gugus residu terminal karboksil dan gugus terminal aminonya. Peningkatan aktifitas enzim dapat disebabkan oleh terjadinya perubahan ionisasi enzim, substrat atau kompleks enzim substrat.

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Extraction and Assays of ADP-glucose Pyrophosphorylase, Soluble Starch Synthase and Granule Bound Starch Synthase from Wheat (Triticum aestivum L.) Grains

BIO-PROTOCOL ◽

10.21769/bioprotoc.1929 ◽

2016 ◽

Vol 6 (18) ◽

Cited By ~ 3

Author(s):

Konstantin Kulichikhin ◽

Shalini Mukherjee ◽

Belay Ayele

Keyword(s):

Triticum Aestivum ◽

Triticum Aestivum L ◽

Soluble Starch ◽

Starch Synthase ◽

Adp Glucose Pyrophosphorylase ◽

Granule Bound Starch Synthase

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Grafting pH‐Responsive Copolymers to Cold Water‐Soluble Starch Using Nitroxide‐Mediated Polymerization

Macromolecular Reaction Engineering ◽

10.1002/mren.202100011 ◽

2021 ◽

pp. 2100011

Author(s):

Alexander T. Fritz ◽

Jaime C. Cazotti ◽

Omar Garcia‐Valdez ◽

Niels M. B. Smeets ◽

Marc A. Dubé ◽

...

Keyword(s):

Cold Water ◽

Soluble Starch ◽

Water Soluble ◽

Ph Responsive ◽

Nitroxide Mediated Polymerization

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Toward an understanding of the biogenesis of the starch granule. Determination of granule-bound and soluble starch synthase functions in amylopectin synthesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)31510-7 ◽

1994 ◽

Vol 269 (40) ◽

pp. 25150-25157

Author(s):

M.L. Maddelein ◽

N. Libessart ◽

F. Bellanger ◽

B. Delrue ◽

C. D'Hulst ◽

...

Keyword(s):

Starch Granule ◽

Soluble Starch ◽

Starch Synthase

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QTL-seq identifies cooked grain elongation QTLs near soluble starch synthase and starch branching enzymes in rice (Oryza sativa L.)

Scientific Reports ◽

10.1038/s41598-019-44856-2 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Siwaret Arikit ◽

Samart Wanchana ◽

Srisawat Khanthong ◽

Chatree Saensuk ◽

Tripop Thianthavon ◽

...

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Soluble Starch ◽

Starch Synthase ◽

Starch Branching Enzymes ◽

Branching Enzymes

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Green synthesis of mesoporous anatase TiO2 nanoparticles and their photocatalytic activities

RSC Advances ◽

10.1039/c7ra08187a ◽

2017 ◽

Vol 7 (76) ◽

pp. 48083-48094 ◽

Cited By ~ 41

Author(s):

Sunderishwary S. Muniandy ◽

Noor Haida Mohd Kaus ◽

Zhong-Tao Jiang ◽

Mohammednoor Altarawneh ◽

Hooi Ling Lee

Keyword(s):

Low Temperature ◽

Green Chemistry ◽

Visible Light ◽

Green Synthesis ◽

Tio2 Nanoparticles ◽

Soluble Starch ◽

Anatase Tio2 ◽

Photocatalytic Activities ◽

Visible Light Active

Mesoporous anatase TiO2 nanoparticles are produced by employing a facile green chemistry approach at low temperature with soluble starch as the template in this work. The obtained TiO2 photocatalyst is visible-light active with good photocatalytic activities.

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OBSERVATIONS ON AMYLOLYTIC BACTERIA: III. CULTURAL CONDITIONS INFLUENCING THE BREAKDOWN OF STARCH BY STENOTHERMOPHILIC BACTERIA BELONGING TO BACILLUS STEAROTHERMOPHILUS

Canadian Journal of Botany ◽

10.1139/b52-028 ◽

1952 ◽

Vol 30 (4) ◽

pp. 360-370 ◽

Cited By ~ 2

Author(s):

Egon Stark ◽

P. A. Tetrault

Keyword(s):

Yeast Extract ◽

Bacillus Stearothermophilus ◽

Soluble Starch ◽

The Other ◽

Ph Changes ◽

Proteose Peptone ◽

Cultural Conditions ◽

Initial Ph ◽

Commercial Medium ◽

Agar Media

Thirty-five cultures of Bacillus stearothermophilus hydrolyzed five starches under various cultural conditions. Hydrolysis occurred regardless of the type, brand, or batch of starch; regardless of the initial pH or of the subsequent pH changes of the medium. Starch in broth was better attacked than in agar media. Some cultures hydrolyzed 0.5%, but not 1% starch; others hydrolyzed easily 10% soluble starch. Length of incubation was important. Certain cultures never formed acid or sugar from starch. Dextrinization was a more reliable indication of starch hydrolysis than was the formation of acid or sugar. Soluble starch gave more consistent results in repeated experiments than did nonsoluble starches. The type of protein medium determines strongly the formation of amylase. Trypticase was the best commercial medium, yeast extract came second. The other 10 media yielded fewer amylolytic cultures. Yeast extract added to media enhanced amylase formation, except with trypticase. Tryptose, proteose-peptone, and neopeptone inhibited the growth of most cultures.

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