Phospho-proteomic analysis of the dopamine pathway enables discovery of a novel reward signal in vivo

2016 ◽  
Vol 4 (Suppl. 2) ◽  
pp. A12.1
Author(s):  
Taku Nagai
Keyword(s):  
Proteomic Analysis

scholarly journals Proteomic analysis of silica hybrid sol-gel coatings: a potential tool for predicting the biocompatibility of implants in vivo

Biofouling ◽  
2017 ◽  
Vol 33 (8) ◽  
pp. 676-689 ◽  
Author(s):  
F. Romero-Gavilan ◽  
A. M. Sánchez-Pérez ◽  
N. Araújo-Gomes ◽  
M. Azkargorta ◽  
I. Iloro ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Sol Gel ◽  
Potential Tool ◽  
Silica Hybrid

Proteomic Analysis of in Vivo 14-3-3 Interactions in the YeastSaccharomyces cerevisiae†

Biochemistry ◽  
2007 ◽  
Vol 46 (26) ◽  
pp. 7781-7792 ◽  
Author(s):  
Kazue Kakiuchi ◽  
Yoshio Yamauchi ◽  
Masato Taoka ◽  
Maki Iwago ◽  
Tomoko Fujita ◽  
...  
Keyword(s):  
Proteomic Analysis

Abstract 362: Proteomic Analysis of Low Oxygen Tension-Induced Secretome of Adipose Tissue-Derived Stem Cells

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Zeljko Bosnjak ◽  
Bassam Wakim ◽  
Yasheng Yan ◽  
Scott Canfield ◽  
Chika Kikuchi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Oxygen Tension ◽  
Proteomic Analysis ◽  
Stem Cell Markers ◽  
Paracrine Factors ◽  
Differentiation Potential ◽  
Trophic Effect ◽  
Lower Oxygen

Growing evidence from animal studies shows that adipose tissue-derived stem cells (ASCs) improve cardiac function of infarcted hearts. It is commonly accepted that therapeutic potential of ASCs may depend more on their paracrine effects than differentiation potential. The underlying mechanisms remain unclear. However, most data regarding paracrine factors were obtained from ASCs cultured in normoxic condition (20%). The present study investigated how in vivo physiological oxygen (4%) tension influenced the secretome of ASCs. ASCs were isolated from three 8-week-old BALB/c mice. ASCs were confirmed by the expression of stem cell markers (CD44 and CD90) and their capacity to differentiate into adipocytes and osteocytes. ASCs at passage 5 were cultured in normoxic (20%) and lower oxygen (4%) incubators and conditioned for 24 h (3 cultures/group). The conditioned media (CM) from ASCs were subjected to trypsin digestion followed by analysis using automated nano-flow liquid chromatography tandem mass spectrometry. The collected LC/MS/MS data were searched against the rodent subset of the Uniprot database and the total proteomes were identified. The data were from 6 technical replicates. A total of 28 proteins were identified and 7 proteins were unique to normoxic CM. Of the 21 common proteins detected in both normoxic and lower oxygen CM, 9 were extracellular matrix proteins. The abundance of 6 of these proteins (e.g., collagen I and laminin) differed noticeably between normoxic and lower oxygen CM. In addition, a greater amount of cytokine CXCL5 and matrix metalloproteinase (MMP)-2 was detected in lower oxygen CM than in normoxic CM while tissue inhibitor of metalloproteinase (TIMP)-1 was only detected in normoxic CM. These results indicate that lower oxygen tension differentially regulates the secretome of ASCs. Extrapolating the results of this study to the in vivo setting, it would appear that injected ASCs may exert their anti-fibrotic and trophic effect by 1) directly regulating the balance of MMP/TIMP production and preventing collagen accumulation in ischemic hearts to decrease fibrosis, and 2) secreting trophic factors including CXCL5. These data suggest that proteomic analysis of CM is useful for elucidation of the paracrine effect of ASCs in vivo.

Proteomic Analysis of Cell Lines and Primary Tumors in Pancreatic Cancer Identifies Proteins Expressed Only In Vitro and Only In Vivo

Pancreas ◽  
2020 ◽  
Vol 49 (8) ◽  
pp. 1109-1116
Author(s):  
Orla Coleman ◽  
Michael Henry ◽  
Fiona O'Neill ◽  
Sandra Roche ◽  
Niall Swan ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cell Lines ◽  
Proteomic Analysis ◽  
Primary Tumors

scholarly journals Proteomic analysis of Proteus mirabilis outer membrane proteins reveals differential expression in vivo vs. in vitro conditions

2011 ◽  
Vol 63 (2) ◽  
pp. 174-182 ◽  
Author(s):  
Bruno D'Alessandro ◽  
Leticia M. S. Lery ◽  
Wanda M. A. Krüger ◽  
Analía Lima ◽  
Claudia Piccini ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Differential Expression ◽  
Proteus Mirabilis ◽  
Proteomic Analysis ◽  
Outer Membrane Proteins

scholarly journals In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins

2013 ◽  
Vol 94 ◽  
pp. 513-526 ◽  
Author(s):  
Eduardo de la Torre-Escudero ◽  
Ricardo Pérez-Sánchez ◽  
Raúl Manzano-Román ◽  
Ana Oleaga
Keyword(s):  
Proteomic Analysis ◽  
Surface Proteins ◽  
Tegumental Surface ◽  
Schistosoma Bovis

scholarly journals Proteomic Analysis Reveals GLUT1 to be a Novel Discriminating Marker of Human Arterial Endothelium In vivo and loss of Venous Identity in Cell Culture

2016 ◽  
Vol 1 (1) ◽  
pp. 001-007
Author(s):  
MR Richardson ◽  
P Cahan ◽  
X Lai ◽  
N Prasain ◽  
MC Yoder
Keyword(s):  
Cell Culture ◽  
Proteomic Analysis ◽  
Arterial Endothelium
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