In order to meet requirement of separation screen for tenebrio molitor L, one separation screen was designed, this product has a good separation, simple structure, low cost, high efficiency, easy operation. Based on the analysis of design requirement of separating screen for tenebrio molitor L, overall structure, working principle and the main structural parameters were determined. The kinematic mathematical model of shaking mechanism was established by using the vector method. The kinematics analysis of shaking mechanism was conducted by using Matlab, the displacement, velocity and acceleration curves of sieve box were obtained. The performance test showed that the prototype machine can screen different instar tenebrio molitor L and frass, this machine has good screening effect. In addition, machine design and performance are able to meet the design requirements.