scholarly journals Gas Chromatography-Mass Spectrometry Profiling of Pimpinella anisum Oils and its Antimicrobial and Antioxidant Activities

2020 ◽  
Vol 11 (02) ◽  
pp. 257-261
Author(s):  
Muthanna J. Mohammed ◽  
Hiyam A. Ebraheem
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Pimpinella Anisum ◽  
Chromatography Mass Spectrometry ◽  
Soxhlet Apparatus

Essential and fixed oils of anise plant Pimpinella anisum growing in Iraq have been investigated regarding their chemical components, antioxidant, and antimicrobial properties. Essential oils were extracted using the Clevenger-apparatus, while fixed oil was extracted using a Soxhlet apparatus. Gas chromatography-mass spectrometry (GC-MS) was used for the analysis of the oil components. Six strains of bacteria, namely S. epidermidis, S. aureus, E. coli, B. cereus, P. vulgaris, and S. typhimurium were tested against the antimicrobial activity of each oil. Anise oil demonstrated a broad antibacterial property range, against gram-positive and gram-negative bacteria, through the inhibition zone. The antibiotic sensitivity test was performed by disk diffusion process against the test organisms. The agar dilution method was used at five different concentrations (12.5, 25, 50, 100, and 200 mg/mL) throughout the test. The minimum inhibitory concentration (MIC) was determined for each volatile and fixed oil. The DPPH radical scavenging assay was used to test the antioxidant activities of essential and fixed oils. Anise oil showed excellent antioxidant activity, in comparison with the reference compounds. Anise oil has the potential to be used as a therapeutic, antimicrobial, and antioxidant agent.

scholarly journals Antioxidant Activities of Dialium indum L. Fruit and Gas Chromatography-Mass Spectrometry (GC-MS) of the Active Fractions

Antioxidants ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 154 ◽  
Author(s):  
Muhamad Osman ◽  
Norazian Mohd Hassan ◽  
Alfi Khatib ◽  
Siti Tolos
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Linoleic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Methanol Fraction ◽  
Chromatography Mass Spectrometry

The fruit of Dialium indum L. (Fabaceae) is one of the edible wild fruits native to Southeast Asia. The mesocarp is consumed as sweets while the exocarp and seed are regarded as waste. This study aimed to evaluate the antioxidant activities of the fruit by using four assays, which measure its capabilities in reducing phosphomolybdic-phosphotungstic acid reagents, neocuproine, 2,2-diphenyl-picrylhydrazyl (DPPH), and inhibiting linoleic acid peroxidation. The active fractions were then analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the seed methanol fraction (SMF) exhibited the strongest antioxidant activity with significantly higher (p < 0.05) gallic acid equivalence (GAE), total antioxidant capacity (TAC), and DPPH radical scavenging activity (IC50 31.71; 0.88 µg/mL) than the other fractions. The exocarp dichloromethane fraction (EDF) was the discriminating fraction by having remarkable linoleic acid peroxidation inhibition (IC50 121.43; 2.97 µg/mL). A total of thirty-eight metabolites were detected in derivatized EDF and SMF with distinctive classes of phenolics and amino acids, respectively. Bioautography-guided fractionation of EDF afforded five antioxidant-enriched subfractions with four other detected phenolics. The results revealed the antioxidant properties of D. indum fruit, which has potential benefits in pharmaceutical, nutraceutical, and cosmeceutical applications.

Volatile Constituents of the Ligarine Extract of Eupatoriurn odoraturn

2013 ◽  
Vol 726-731 ◽  
pp. 245-249
Author(s):  
Zhong Liang Sun ◽  
Feng Xia Liu ◽  
Xian Qun Luo ◽  
Yu Cang Zhang ◽  
Jing Xu
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Eicosatrienoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Constituents ◽  
Aerial Parts ◽  
Chromatography Mass Spectrometry ◽  
Main Components

Eupatoriurn odoratumaerial parts were extracted with ligarine and the volatile constituents isolated were analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Volatile constituents were isolated from the ground aerial parts ofE. odoratumby ligarine extraction and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). As a result, a total of fifteen compounds represented all of the extract were identified, amongst nine of fifteen compounds were sesquiterpenes. Esters and sesquiterpenes were found to compose three major chemotype accounted for 58.58% and 30.80% of the constituents, respectively. The main components was demonstrated to be dibutyl phthalate (39.73%), 11,14,17-eicosatrienoic acid, methyl ester (13.20%), (S)-spiro [4.nona-1,6-diene (6.80%), 1,2-benzenedicarboxylic acid, bis (2-methylpropyl) ester (5.65%) andcis-Z-α-bisabolene epoxide (5.56%). In addition, some pharmaceutical components such as α-cadinol and germacrene D were discovered. Antioxidant activity of the extract was assessed by the free radical scavenging (DPPH). The study offers theoretic basis for pharmaceutical utilization of the medicinal plantE. odoratum.

Measurement of blood volume with an enriched stable isotope of chromium (53Cr) and isotope dilution by combined gas chromatography-mass spectrometry

1994 ◽  
Vol 40 (1) ◽  
pp. 71-73 ◽  
Author(s):  
C Veillon ◽  
K Y Patterson ◽  
D A Nagey ◽  
A M Tehan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Blood Volume ◽  
Isotope Dilution ◽  
Gas Chromatography Mass Spectrometry ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
Chromatography Mass Spectrometry ◽  
Survival Studies

Abstract We used an established isotope dilution gas chromatography-mass spectrometry method to measure blood volumes with an enriched stable isotope of chromium. The results were compared with those of the conventional method, which involves radioactive 51Cr. The two methods were compared simultaneously in two male subjects of different sizes, and essentially identical volumes were obtained. The isotope dilution method is insensitive to contamination with natural (unenriched) chromium and can be used to measure all of the stable isotopes of chromium. The method has potential applications in simultaneous erythrocyte survival studies. The absence of radioactivity makes possible an accurate means of studying blood volume changes in children and during pregnancy.

scholarly journals ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES

2019 ◽  
Vol 12 (1) ◽  
pp. 289 ◽  
Author(s):  
Samreen Fatema ◽  
Milind Ubale ◽  
Mazahar Farooqui ◽  
Pathan Mohd Arif
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Results ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Study

Objective: The main objective of the present work is to carry out the biological activity, gas chromatography-mass spectrometry (GC-MS) studies for the possible compounds present in Vitex negundo.Methods: The aqueous extract of V. negundo Linn. was screened for biological activities such as antimicrobial, antituberculosis (TB), antimalarial, and antioxidant activities. The GC-MS analysis was carried out.Results: The result shows that leaf extract is effective against Escherichia Coli and Bacillus subtitus while negative results for anti-TB and anti-malarial activity. The antioxidant activity of the leave extract is excellent.Conclusion: The compounds present in the leaf extract of V. negundo are responsible for possessing the biological activity.

scholarly journals PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY–MASS SPECTROMETRY PROFILING, IN VITRO CYTOTOXICITY, AND ANTHELMINTIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF DURANTA ERECTA LEAVES

2019 ◽  
pp. 63-68
Author(s):  
RAJESWARI R ◽  
PRABHAKARAN V ◽  
SARAVANAKUMAR A
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Anthelmintic Activity ◽  
In Vitro Cytotoxicity ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroalcoholic Extract ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Soxhlet Apparatus

Objective: The present study aims at the preliminary phytochemical evaluation, gas chromatography–mass spectrometry (GC–MS) analysis, in vitro cytotoxicity, and anthelmintic activity of the hydroalcoholic extract of D. erecta leaves. Methods: The hydroalcoholic extraction of the plant material is done with Soxhlet apparatus and the GC–MS profiling of the extract is performed. The in vitro cytotoxicity activity of the extract is done using the MOLT-3 cell lines. The anthelmintic activity is performed using Pheretima posthuma. Results: The GC–MS analysis shows the presence of various secondary metabolites with proved pharmacological activity. The in vitro cytotoxicity and the anthelmintic activity of the extract show the beneficial effect of the plant. Conclusion: The flavonoids, phenolic compounds, and tannins present in the plant extract may support the cytotoxic and anthelmintic activity of the plant.

Essential fatty acid components and antioxidant activities of eight Cephalaria species from southwestern Anatolia

2007 ◽  
Vol 79 (12) ◽  
pp. 2297-2304 ◽  
Author(s):  
Süheyla Kırmızıgül ◽  
Nazlı Böke ◽  
Hüseyin Sümbül ◽  
R. Süleyman Göktürk ◽  
Nazlı Arda
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Fatty Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Methyl Esters ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Soxhlet Apparatus ◽  
Hexane Extracts

The hexane extracts of eight Cephalaria (Dipsacaceae) species, which were collected from southwestern Anatolia, were obtained by Soxhlet apparatus. The fatty acids were derived to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The dominant fatty acid components and maximum percentages were detected as myristic [in C. joppica (17.48 %)], palmitic [in C. cilicica, C. elmaliensis, C. isaurica, C. scoparia (19.51 %), and C. gazipashaensis], linoleic [in C. joppica (33.02 %), C. elmaliensis, C. dipsacoides, and C. gazipashaensis], α-linolenic (ALA) [in C. cilicica, C. elmaliensis, C. isaurica, C. scoparia, C. lycica, and C. gazipashaensis (47.95 %)] and oleic [in C. isaurica and C. dipsacoides (40.66 %)] acids. The antioxidant activity of all hexane extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric thiocyanate (FTC), and thiobarbituric acid (TBA) methods. The results indicate that hexane extracts of Cephalaria species possess considerable antioxidant activity. The highest radical scavenging activity was detected in C. isaurica (IC50 = 741 μg/mL). The most effective species on lipid peroxidation are C. lycica and C. gazipashaensis in FTC and TBA assays, respectively. This study reveals that Cephalaria species are attractive sources of fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

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