scholarly journals Amplificación cruzada de microsatélites en dos especies de haemúlidos (Haemulon aurolineatum y Haemulon steindachneri)

2018 ◽  
Vol 47 (1) ◽  
Author(s):  
Paula E. Pabón Quintero ◽  
José Julian Tavera ◽  
Ana María Millán-Márquez ◽  
Arturo Acero P.
Keyword(s):  
Population Genetics ◽  
Molecular Markers ◽  
Gene Flow ◽  
Marine Fish ◽  
Population Studies ◽  
Artisanal Fisheries ◽  
Genetic Population ◽  
Genetic Studies ◽  
Future Studies ◽  
Cross Amplification

Genetic-population studies in marine fish have allowed to study patterns of dispersal and connectivity between habitats. Oneimportant tool in population genetics is the use of microsatellite molecular markers. Cross-amplification of microsatellite is amethod that consists in using primers designed for one species in a different one but phylogenetically related. Because of theimportance of genetic studies of populations in artisanal fisheries species, primers were evaluated and designed for the species Haemulon aurolineatum and Haemulon steindachneri. Samples were collected from the artisanal fisheries in Barú-Colombia. Amplification was standardized for 12 microsatellites which ten were polymorphic for H. aurolineatum and nine for H. steindachneri. It is considered that the primers implemented in this study are useful for future studies of gene flow in these species.

scholarly journals AMPLIFICACIÓN CRUZADA DE MICROSATÉLITES PARA ESTUDIOS POBLACIONALES DE DOS ESPECIES DE CAMARÓN DEL GÉNERO LITOPENAEUS EN COLOMBIA

2016 ◽  
Vol 42 (1) ◽  
Author(s):  
Adriana Fresneda Rodríguez ◽  
Luis Chasqui Velasco ◽  
David Alonso Carvajal
Keyword(s):  
Population Genetics ◽  
Molecular Markers ◽  
Population Genetic ◽  
Dna Amplification ◽  
High Specificity ◽  
Target Species ◽  
Genetic Studies ◽  
Litopenaeus Setiferus ◽  
Cross Amplification ◽  
Long Time

Microsatellites are molecular markers frequently used in population genetic studies despite of the high cost, and long time involved in developing them, mainly due to their high specificity. One method to save money and time is cross-amplification, which is the DNA amplification of the target species using primers developed for a different species. By using cross-amplification, the suitability of 15 developed microsatellite loci from Litopenaeus setiferus and L. vannamei to amplify microsatellite regions of L. schmitti and L. occidentalis was evaluated. Five primers showed consistent amplification and were polymorphic in L. schmitti and four in L. occidentalis. These results point out the usefulness of cross-amplification with these primers for population genetics studies of both species.

scholarly journals Molecular markers applying in forest trees gene pool conservation

2009 ◽  
pp. 101-113
Author(s):  
Jelena Milovanovic ◽  
Mirjana Sijacic-Nikolic
Keyword(s):  
Population Genetics ◽  
Molecular Markers ◽  
Gene Flow ◽  
Gene Pool ◽  
Statistical Power ◽  
Forest Trees ◽  
Effective Population ◽  
Migration Patterns ◽  
Geographic Diversity ◽  
Marker Loci

Many studies performed during the last years demonstrated the usefulness of neutral molecular markers in the field of conservation and population genetics of forest trees, in particular to understand the importance of migration patterns in shaping current genetic and geographic diversity and to measure important parameters such as effective population size, gene flow and past bottleneck. During the next years, a large amount of data at marker loci or at sequence level is expected to be collected, and to become excellent statistical power for the assessment of biological and evolutionary value.

scholarly journals Novel microsatellites for Cypseloides fumigatus, cross-amplifiable in Streptoprocne zonaris

2019 ◽  
Vol 27 (3) ◽  
pp. 207-211
Author(s):  
Renata Neves Biancalana ◽  
Fabio Raposo do Amaral ◽  
Cibele Biondo
Keyword(s):  
Microsatellite Markers ◽  
Related Species ◽  
New World ◽  
Population Studies ◽  
Genetic Population ◽  
World Species ◽  
Cross Amplification ◽  
The Cross ◽  
Species Cross

AbstractBased on microsatellite prospection, we isolated and characterized 21 microsatellite markers for the Sooty Swift (Cypseloides fumigatus) and tested the cross-amplification in the White-collared Swift (Streptoprocne zonaris). Both species are New World species included in the Apodidae family. From these 21, only 13 loci were polymorphic in the Sooty Swift, and their levels of polymorphism were surprisingly low compared to related species. Cross-amplification in the White-collared Swift was successful for 11 loci of the 13 polymorphic found for the Sooty Swift, but seven were monomorphic and four were biallelic. The microsatellites described here could be useful in future genetic population studies for Sooty Swifts and related species.

Indirect methods for monitoring and modeling gene flow in natural plant populations.

2021 ◽  
pp. 26-38
Author(s):  
María Eugenia Barrandeguy ◽  
María Victoria García
Keyword(s):  
Molecular Markers ◽  
Gene Flow ◽  
Population Genetic ◽  
Molecular Ecology ◽  
Evolutionary Process ◽  
Genetic Admixture ◽  
Indirect Methods ◽  
Genetic Studies ◽  
Approximate Bayesian ◽  
Sequencing Platforms

Abstract Gene flow is a micro-evolutionary process that maintains the allelic exchange among local populations, increasing population genetic diversity. Because of the immobility of plants, pollen plays a major role in connecting extant populations while seeds are necessary to establish and maintain populations of plants. Despite the widespread use of next-generation sequencing platforms, co-dominant molecular markers, such as microsatellites, are still useful and informative tools in molecular ecology and conservation genetic studies. These markers are currently the most frequently used tools for population genetic studies in plants. These molecular markers reveal information for gene flow estimation by indirect methods and also for comparing the role of gene flow by pollen versus gene flow by seeds in the determination of population genetic structures. Approximate Bayesian computation methods are often used to determine the most probable model of genetic admixture among populations.

Genetic population studies on 15 NGM™ STR loci in central Poland population

2012 ◽  
Vol 6 (4) ◽  
pp. e119-e120 ◽  
Author(s):  
Maciej Jedrzejczyk ◽  
Renata Jacewicz ◽  
Stefan Szram ◽  
Jaroslaw Berent
Keyword(s):  
Population Studies ◽  
Genetic Population ◽  
Str Loci ◽  
Central Poland

scholarly journals Genetic structure and diversity of the mustard hill coral Porites astreoides along the Florida Keys reef tract

2021 ◽  
Vol 51 (4) ◽  
Author(s):  
Dominique N. Gallery ◽  
Michelle L. Green ◽  
Ilsa B. Kuffner ◽  
Elizabeth A. Lenz ◽  
Lauren T. Toth
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Relatedness ◽  
Florida Keys ◽  
Genetic Population ◽  
High Genetic Diversity ◽  
Western Atlantic ◽  
Porites Astreoides ◽  
Single Well ◽  
Reef Tract

AbstractIncreases in local and global stressors have led to major declines in coral populations throughout the western Atlantic. While abundances of other species have declined, however, the relative abundance of the mustard hill coral, Porites astreoides, has increased. Porites astreoides is relatively resilient to some stressors, and because of its mixed reproductive strategies, its populations often recover quickly following disturbances. The ability for P. astreoides to continue as a potential “winner” in western Atlantic reefs relies on maintaining sufficient genetic variation within populations to support acclimatization and adaptation to current and future environmental change. Without high genetic diversity and gene flow within the population, it would have limited capacity for adaptation and the species’ competitive advantages could be short-lived. In this study, we determined the genetic relatedness of 37 P. astreoides colonies at four shallow reefs along the offshore Florida Keys Reef Tract (FKRT), a region particularly hard-hit by recent disturbances. Using previously designed microsatellite markers, we determined the genetic diversity and connectivity of individuals among and between sites. Our results suggest that the FKRT likely contains a single, well-mixed genetic population of P. astreoides, with high levels of gene flow and evidence for larval migration throughout the region. This suggests that regional populations of P. astreoides likely have a higher chance of maintaining resilience than many other western Atlantic species as they face current and future disturbances.

scholarly journals Population genetics of Southern Hemisphere tope shark (Galeorhinus galeus): Intercontinental divergence and constrained gene flow at different geographical scales

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184481 ◽  
Author(s):  
Aletta E. Bester-van der Merwe ◽  
Daphne Bitalo ◽  
Juan M. Cuevas ◽  
Jennifer Ovenden ◽  
Sebastián Hernández ◽  
...  
Keyword(s):  
Population Genetics ◽  
Gene Flow ◽  
Southern Hemisphere ◽  
Galeorhinus Galeus
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