Effect of dietary omega-3 fatty acid source on plasma and red blood cell membrane composition and immune function in yearling horses1

The present study evaluated the effects of moderate intakes of myristic acid (MA), at 1·2% and 1·8% of total energy (TE), associated with a 0·9% TE intake of α-linolenic acid (ALA) on lipid and fatty acid profiles and red blood cell membrane fluidity. Twenty-nine monks without dyslipidaemia were enrolled in a 1-year nutritional study in which two experimental diets were tested for 3 months each: diet 1, MA 1·2% and ALA 0·9%; diet 2, MA 1·8% and ALA 0·9%. A control diet (MA 1·2%, ALA 0·4%) was given 3 months before diets 1 and 2. Thus, two different levels of MA (1·2%, 1·8%) and ALA (0·4%, 0·9%) were tested. Intakes of other fatty acids were at recommended levels. Samples were obtained on completion of all three diets. For fluidity analysis, the red blood cells were labelled with 16-doxylstearate and the probe incorporated the membrane where relaxation-correlation time was calculated. Diet 1 was associated with a decrease in total cholesterol, in LDL-cholesterol, in triacylglycerols and in the ratio of total to HDL-cholesterol; ALA and EPA levels were increased in both phospholipids and cholesterol esters. Diet 2 was associated with a decrease in triacylglycerols and in the ratios of total to HDL-cholesterol and of triacylglycerols to HDL-cholesterol, and with an increase in HDL-cholesterol; EPA levels were decreased in phospholipids and cholesterol esters. Red blood cell membrane fluidity was increased in both diets (P<0·0001), but the higher increase was obtained with diet 1, mainly in the oldest subjects. Intakes of myristic acid (1·2%TE) and ALA (0·9%TE), both mainly in the sn-2 position, were associated with favourable lipid and n−3 long-chain fatty acid profiles. These beneficial effects coexisted with particularly high membrane fluidity, especially among the oldest subjects.

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Acute Coronary Syndrome Patients With Depression Have Low Blood Cell Membrane Omega-3 Fatty Acid Levels

Psychosomatic Medicine ◽

10.1097/psy.0b013e318188a01e ◽

2008 ◽

Vol 70 (8) ◽

pp. 856-862 ◽

Cited By ~ 35

Author(s):

Alpesh A. Amin ◽

Rishi A. Menon ◽

Kimberly J. Reid ◽

William S. Harris ◽

John A. Spertus

Keyword(s):

Acute Coronary Syndrome ◽

Fatty Acid ◽

Cell Membrane ◽

Blood Cell ◽

Omega 3 ◽

Omega 3 Fatty Acid ◽

Coronary Syndrome

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FATTY ACID COMPOSITION OF RED BLOOD CELL MEMBRANE PHOSPHATIDYLETHANOLAMINE AND PHOSPHATIDYLCHOLINE IN RAT, RABBIT, HUMAN AND DOG : SIÇAN, TAVŞAN, İNSAN VE KÖPEK ERİTROSİT MEMBRANI FOSFATİDİLETANOL

Ankara Universitesi Eczacilik Fakultesi Dergisi ◽

10.1501/eczfak_0000000374 ◽

2002 ◽

pp. 169-182

Author(s):

Bilgehan DOĞRU PEKİNER

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Cell Membrane ◽

Blood Cell ◽

Acid Composition ◽

Red Blood Cell ◽

Red Blood Cell Membrane

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Oxidative Stress, Nutrient Intake, and Red Blood Cell Membrane Fatty Acid Profiles of Women with and without Fertility Issues

The FASEB Journal ◽

10.1096/fasebj.29.1_supplement.lb263 ◽

2015 ◽

Vol 29 (S1) ◽

Author(s):

Nicole Litwin ◽

Norman Assad ◽

W. Andrew Clark ◽

Ray Mohseni ◽

Shimin Zheng ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Acid ◽

Cell Membrane ◽

Blood Cell ◽

Red Blood Cell ◽

Nutrient Intake ◽

Membrane Fatty Acid ◽

Fatty Acid Profiles ◽

Red Blood Cell Membrane

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Abstract 397: Correlation Between the Whole Blood Omega-Score and Red Blood Cell Membrane Omega-3 Index in a Patient Population with Cardiovascular Disease Risk Factors Treated With a Unique Omega-3 Formulation

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.34.suppl_1.397 ◽

2014 ◽

Vol 34 (suppl_1) ◽

Author(s):

George Jackowski ◽

Jason Yantha ◽

Petro Kupchak ◽

Nisar A Shaikh

Keyword(s):

Risk Factors ◽

Comparative Analysis ◽

Cell Membrane ◽

Blood Cell ◽

Red Blood Cell ◽

Whole Blood ◽

Disease Risk ◽

Cardiovascular Disease Risk ◽

Omega 3 ◽

Red Blood Cell Membrane

Background: Growing evidence supports the value of maintaining a diet rich in omega-3 polyunsaturated fatty acids (PUFA) to achieve longevity of cardiovascular health, and prevent sudden death. Omega-3 deficiency (OM3D) is highly prevalent in the US and contributes to 84,000 deaths/year. To identify patients at risk with OM3D, two laboratory tests are available, including the Omega-Score (OS - whole blood), as well as the Omega-3 Index (OI - red blood cell membrane), that measure omega-3 blood levels. Omega-Score or OI levels <6.1% suggest OM3D. Here we report on the dietary omega-3 status of Canadians, including those with CVD risk factors, through a comparative analysis of the OS vs. OI both pre- and post-treatment with a unique omega-3 formulation. Methods: Open label study of 143 study subjects enrolled for baseline omega-3 deficiency assessment, of which 63 subjects were scheduled to receive a 4 g/day regimen of a highly purified (>90%) omega-3 formulation (2720 mg/day of EPA + 440 mg/day of DHA), a ratio of 6:1 EPA:DHA (6:1 OM-3) for two weeks, and 31 subjects received 6:1 OM-3 for six weeks. Diagnostic evaluation of the OS, and OI was carried out at baseline, and every two weeks until the study completion. Results: The majority (84.5%) of subjects tested at baseline had OS levels <6.1%. The baseline, mean OS and OI levels were 3.4% and 4.4% respectively (N=143). After six weeks of treatment, a significant improvement in the OS (120.6% increase, p<0.0001), and OI (72.7% increase, p<0.0001) was observed (N=31). Comparative analysis of the OS vs. the OI resulted in comparable values, with a greater baseline OI, but with near-identical resultant OS an OI levels after six weeks of treatment (7.5%, vs. 7.6% respectively). Conclusions: This study shows that both the OS and OI provide highly correlative results in the assessment of nutritional omega-3 deficiency, and response to treatment with 6:1 OM-3.

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