In Vivo Evaluation of a Brown Midrib Mutant of Zea Mays L.

1972 ◽  
Vol 35 (4) ◽  
pp. 883-889 ◽  
Author(s):  
L. D. Muller ◽  
V. L. Lechtenberg ◽  
L. F. Bauman ◽  
R. F. Barnes ◽  
C. L. Rhykerd
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Brown Midrib ◽  
In Vivo Evaluation

Utilization of Cogon (Imperata cylindrica L.) Silage and Urea-Treated Corn (Zea mays L.) Stover as Affected by Supplementation

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Elmar Patiga ◽  
◽  
Lolito Bestil ◽  
Hershey Mondejar ◽  
◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Imperata Cylindrica

In vivo, urea-treated, silage, concentrate: Ipil-ipil supplement

scholarly journals Baked corn (Zea mays L.) and cooked common bean (Phaseolus vulgaris L.) chips improved enzymatic biomarkers and alleviated inflammation during chronic colitis in vivo (P06-063-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Ivan Luzardo ◽  
Rocio Campos-Vega ◽  
Elvira Gonzalez de Mejia ◽  
Flavia Loarca
Keyword(s):  
Zea Mays ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Zea Mays L ◽  
Relative Weight ◽  
Inflammatory Cells ◽  
Phaseolus Vulgaris L ◽  
Chronic Colitis ◽  
Gut Barrier Function

Abstract Objectives The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the modulation of infiltration and adhesion of inflammatory cells. Methods A 70% corn and 30% bean chip (7030C) was evaluated, using dextran sodium sulfate (DSS, 2% v/v) as chemical inductor of colitis. After 1-week of acclimatization, 36 CD-1 male mice (6–8 weeks age) were randomly divided into 5 groups: G1 (negative control, fed with basal diet, BD, and water); G2 (positive control, BD + DSS), G3 (100 g 7030C/kg body weight, BW/day + BD), G4 (200 g 7030C/kg BW + BD) and G5 (300 g 7030C/kg BW + BS). The G2-G5 groups were administered DSS every other week, during 5-weeks.After the mice were euthanized, BW and disease activity index (DAI) were recorded. Liver, colon, and spleen were collected, weighed and analyzed for histology. Colonic myeloperoxidase (MPO)/fecal b-glucuronidase (GLUC) activities were also quantified, as well as fecal/cecal metabolites. The colonic mRNA expression of inflammation-associated genes was conducted using a gene inflammation profiler array. Results DSS increased DAI up to 2 units, BW loss was 10–17%, and induced colon shortening 10–15%. Compared to G2, G4 exhibited significantly (p < 0.05) lower DAI (0.75 ± 0.01), spleen relative weight (0.003 ± 0.0001) and colon weight/length ratio (0.045 ± 0.008). The histological analysis showed that the chip consumption prevented colonic barrier damage. G4 displayed the lowest MPO and GLUC among all DSS-induced groups (0.004 ± 0.0004 mU/mg colon; 0.44 ± 0.01 mmol/min/g feces, respectively), and the lowest seric content of MCP-1 protein. Amid all the quantified metabolites, the chip consumption significantly reduced the fecal/cecal content of acetic acid, while butyric and propionic increased at the end of the study. Inflammation gene expression was modulated by the chip consumption. Conclusions Our results suggest that the consumption of this chip might alleviate chronic colitis symptoms because of a protective effect in the gut barrier function and the modulation of infiltration of inflammatory cells. Funding Sources The funding received by CONACyT, CONCyTEQ-Mexico and NIFA-USDA-HATCH are appreciated.

Genetic and physical fine mapping of the novel brown midrib gene bm6 in maize (Zea mays L.) to a 180 kb region on chromosome 2

2012 ◽  
Vol 125 (6) ◽  
pp. 1223-1235 ◽  
Author(s):  
Yongsheng Chen ◽  
Hongjun Liu ◽  
Farhad Ali ◽  
M. Paul Scott ◽  
Qing Ji ◽  
...  
Keyword(s):  
Zea Mays ◽  
Fine Mapping ◽  
Zea Mays L ◽  
Brown Midrib ◽  
Chromosome 2 ◽  
The Novel

scholarly journals Development of a New Maize (Zea mays L.) Breeding Program

2002 ◽  
pp. 25-30
Author(s):  
Pál Pepó ◽  
Szilárd Tóth
Keyword(s):  
Zea Mays ◽  
Recurrent Selection ◽  
Zea Mays L ◽  
Genetic Manipulation ◽  
Dominant Gene ◽  
Breeding Program ◽  
Regeneration Ability ◽  
Breeding Programs

Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.

scholarly journals Evaluation of the in vitro and in vivo antihypertensive effect and antioxidant activity of blue corn hydrolysates derived from wet-milling//Evaluación del efecto antihipertensivo in vitro e in vivo y actividad antioxidante del hidrolizado de maíz azul derivado de la molienda húmeda

Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Alvaro Montoya-Rodríguez ◽  
Evelyn Isabel Osuna-Gallardo ◽  
Francisco Cabrera-Chávez ◽  
Jorge Milán-Carrillo ◽  
Cuauhtémoc Reyes-Moreno ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Antioxidant Activity ◽  
Zea Mays ◽  
Antihypertensive Effect ◽  
Zea Mays L ◽  
Enzymatic Digestion ◽  
Wet Milling ◽  
Food Ingredient

Hypertension is considered a risk factor for coronary heart disease, and its prevalence has increased substantially. Inhibition of angiotensin-converting enzyme (ACE-I) is key to lower blood pressure, making it an excellent treatment for hypertension. Corn (Zea mays L.) is an important source of bioactive peptides with potential anti-hypertensive activity related to ACE-I inhibition. These peptides can be obtained through the hydrolysis of corn gluten meal (CGM), as wetmilling by-products. The aim was to evaluate the in vitro and in vivo ACE-I inhibitory activity of blue CGM hydrolysates. Enzymatic digestion in vitro of blue CGM was conducted at different times. Hydrolysis for 360 min significantly increased both soluble protein and antioxidant activity by 4 and 8-fold respectively, the maximum ACE-I inhibition (94.3 %) was observed with 260 min hydrolysate. Mice were treated with the blue CGM hydrolysate (260 min), captopril or PBS to test the bioavailability in vivo. The CGM hydrolysate was detected in serum after 5 and up to 30 min after ingestion, showing the maximum ACE-I inhibitory capacity (59 %) during the first 15 min. Overall, this work showed that the blue CGM hydrolysate could serve as a functional food ingredient with antihypertensive effect due to its blood pressure-lowering peptides.RESUMENLa hipertensión es factor de riesgo en enfermedades coronarias, y su prevalencia ha aumentado sustancialmente. La inhibición de enzima convertidora de angiotensina (ECA) es clave para disminuir presión arterial, y excelente tratamiento para hipertensión. El maíz (Zea mays L.) es fuente de péptidos bioactivos con actividad antihipertensiva por inhibición de ECA. Péptidos pueden obtenerse por hidrólisis de harina de gluten de maíz (HGM), como subproducto de molienda húmeda. El objetivo fue evaluar in vitro e in vivo actividad inhibitoria de ECA en hidrolizados de HGM azul. La digestión enzimática in vitro de HGM fue conducida a diferentes tiempos. La hidrólisis por 360 min aumento significativamente proteína soluble y actividad antioxidante de 4 y 8 veces, respectivamente; la máxima inhibición de ECA (94.3 %) fue observada a 260 minutos del hidrolizado. Ratones fueron tratados con HGM hidrolizado (260 minutos), captopril o PBS para evaluar biodisponibilidad in vivo. Después de la ingestión, HGM hidrolizado fue detectado en suero en 5 hasta 30 minutos, mostrando máxima inhibición de ECA (59 %) durante los primeros 15 minutos. En general, este trabajo mostró que hidrolizado de HGM podría servir como ingrediente funcional en alimentos con efecto antihipertensivo debido a péptidos reductores de presión arterial.

In Vitro dry Matter Disappearance of Brown Midrib Mutants of Maize (Zea Mays L.)

1971 ◽  
Vol 33 (4) ◽  
pp. 881-884 ◽  
Author(s):  
R. F. Barnes ◽  
L. D. Muller ◽  
L. F. Bauman ◽  
V. F. Colenbrander
Keyword(s):  
Zea Mays ◽  
Dry Matter ◽  
Zea Mays L ◽  
Brown Midrib

Safeners as Corn Seedling Protectants against Acetolactate Synthase Inhibitors

1991 ◽  
Vol 46 (11-12) ◽  
pp. 945-949
Author(s):  
Henri Milhomme ◽  
Christophe Roux ◽  
Jean Bastide
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Acetolactate Synthase ◽  
Large Degree ◽  
Naphthalic Anhydride ◽  
Metsulfuron Methyl ◽  
Corn Seedling

Zea mays L., cv. Potro, shoots had a higher level of resistance to imazaquin (IQ) and metsulfuron methyl (M SM ) than roots. Shoot lengths were increased by pretreating the seeds with commercial 1,8 -naphthalic anhydride (N A ) at 1% (w/w) or oxabetrinil at 0.2% (w/w). The growth of shoots of safened seeds was unaffected by 400 nM IQ and by 40 nM MSM. The in vivo activity of acetolactate synthase (ALS) extracted from corn shoots and roots was not affected by treatments with IQ or MSM, but pretreatment of seeds with NA or oxabetrinil, prior to germination, caused an increase in the level of extractable ALS from shoots. ALS activity from roots and shoots of NA-pretreated seedlings was increased to a large degree (> 40% ) when the seedlings were germinated on 40 nM MSM, whereas ALS activities from oxabetrinil-pretreated seedlings were enhanced to a lesser degree (≈20%). ALS from unsafened seedlings was inhibited 21% by 400 nM IQ and 70% by 40 nM MSM in vitro, but ALS from roots of seedlings germinated on 400 nM IQ was not inhibited by 400 nM IQ in vitro.

scholarly journals ÓLEO ESSENCIAL DE ERVA-CIDREIRA SOBRE FITOPATÓGENOS DE ESPÉCIES FLORESTAIS E AGRÍCOLAS

2018 ◽  
Vol 10 (1) ◽  
pp. 206
Author(s):  
Bruna Cristine Martins de Sousa ◽  
Santana Pinto de Castro ◽  
Katiane Araújo Lourido ◽  
David Chiara ◽  
Kelly Christina Ferreira Castro ◽  
...  
Keyword(s):  
Zea Mays ◽  
Glycine Max ◽  
Rhizoctonia Solani ◽  
Capsicum Annuum ◽  
Zea Mays L ◽  
Fusarium Spp ◽  
Capsicum Annuum L ◽  
Lippia Alba ◽  
Glycine Max L

Pertencente à família Verbenaceae, Lippia alba (Mill) N. E. Brown é amplamente utilizada na medicina popular devido suas propriedades analgésicas, calmantes e espasmolíticas. Neste trabalho, buscou-se avaliar o efeito do óleo essencial dessa espécie sobre o crescimento micelial de isolados de Fusarium spp. obtidos de Anacardium occidentale L. (caju); Zea mays L. (milho); Capsicum annuum L. (pimentão); Couratari oblongifolia Ducke & R. Knuth (tauari) e Rhizoctonia solani obtido da espécie Glycine max L. (soja). No bioensaio, o óleo previamente filtrado foi adicionado em meio BDA (Batata-Dextrose-Ágar), homogeneizado e vertido em placas de Petri. Em seguida, discos contendo estruturas fúngicas foram depositados, centralmente, nas placas com BDA e o óleo em suas respectivas concentrações. O tratamento controle consistiu em placas com meio de cultura sem a presença do óleo. Os dados foram obtidos medindo o diâmetro das colônias fúngicas, durante cinco dias. Todas as concentrações reduziram o crescimento micelial dos fitopatógenos em relação à testemunha, apresentando um percentual de inibição médio de 88,5%. Para os isolados de Fusarium spp. do caju e do pimentão, bem como Rhizoctonia solani isolado da soja, o óleo apresentou efeito fungicida; sugerindo estudos, in vivo, para constatação de seu efeito fungitóxico, e análises químicas para investigação e isolamento dos compostos ativos da espécie.PALAVRAS-CHAVE: Controle alternativo, Efeito fungicida, Redução do crescimento.

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