Possibility of application different cryopreservatives for tissue-engineered corneal constructions storage

Purpose. Conduct a comparative analysis of corneal tissue–engineered constructs that have undergone cryopreservation of three different cryoprotectants. Materials and methods. After refractive surgery Relex SMILE, corneal tissue was obtained which is called a lenticule. Corneal tissue engineering constructs (RTK) have created using decellularization methods that relate to the technologies of tissue and regenerative medicine. For decellularization of the lenticules, a protocol with a 1.5 M solution of Sodium Chloride with DNase 5 U / ml and RNase 5 U / ml was used. After decellularization, RTK was cryopreserved using: 1) 10% DMSO and 90% “corneal storage solution” (Borzenok–Moroz medium), 2) «Cryoderm» cell cryopreservation medium, 3) 100% glycerin. Transparency was assessed using spectrophotometry. The thickness of collagen fibers was assessed by scanning electron microscopy (SEM). Results. When assessing the transparency of the control and experimental groups, a statistically significant decrease in transparency was revealed in the groups using cryoprotectants – Glycerin and Cryoderm (p<0.001), the groups using DMSO did not differ from the control (p=0.99). Also, statistically significant differences were found between the experimental groups «Cryoderm»–DMSO (p=0.02), «Cryoderm»–Glycerin (p<0.001) and Glycerin–DMSO (p<0.001). When evaluating the SEM data, it was found that the thickness of collagen fibers did not differ in the DMSO–Control and Cryoderm–Control groups (p>0.05). The Glycerol–Control group was statistically different (p<0.001). Conclusions. In our work, we have shown the use of DMSO for storing RTK causes thickening of collagen fibers after decellularization of similar to native lenticules. This protocol can be useful for long–term storage and creation of the RTK cryobank. Key words: storage, lenticule, cryoprotectants, cryopreservation, cornea.

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Chemical treatment of papaya seeds aiming at long-term storage and control of damping off

Revista CERES ◽

10.1590/s0034-737x2014000300013 ◽

2014 ◽

Vol 61 (3) ◽

pp. 384-391

Author(s):

Silvia de Carvalho Campos Botelho ◽

Silvaldo Felipe da Silveira ◽

Roberto Ferreira da Silva ◽

Alexandre Pio Viana

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Seed Vigor ◽

Control Group ◽

Damping Off ◽

Effective Measure ◽

Long Term Storage ◽

Papaya Seeds ◽

Term Storage

Damping off is a nursery disease of great economic importance in papaya and seed treatment may be an effective measure to control. The aim of this work was to evaluate the quality of papaya seeds treated with fungicides and stored under two environmental and packaging conditions. Additionally, the efficiency of fungicide treatments in the control of damping-off caused by Rhizoctonia solani was evaluated. Papaya seeds were treated with the fungicides Captan, Tolylfluanid and the mixture Tolylfluanid + Captan (all commercial wettable powder formulations). Seeds of the control group were not treated. The seeds were stored for nine months in two conditions: packed in aluminum coated paper and kept at 7 ± 1ºC and in permeable kraft paper and kept in non-controlled environment. At the beginning of the storage and every three months the seed quality (germination and vigor tests), emergence rate index, height, dry mass and damping of plants in pre and post-emergence (in contaminated substrate and mycelia-free substrate) were analyzed. Both storage conditions as well as the fungicide treatments preserved the germination and seed vigor. In the infested substrate, seedling emergence was favored by fungicides, but in post-emergence, fungicides alone did not control the damping off caused by R. solani. Symptoms of damping off were not observed in the clean substrate. The results showed that the fungicide treatments may be used to pretreat papaya seed for long-term storage and commercialization.

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Influence of Frozen Storage Duration and Forcing Temperature on Flowering of Oriental Hybrid Lilies

HortScience ◽

10.21273/hortsci.36.6.1053 ◽

2001 ◽

Vol 36 (6) ◽

pp. 1053-1056 ◽

Cited By ~ 20

Author(s):

Jong Suk Lee ◽

Mark S. Roh

Keyword(s):

Air Conditioning ◽

Floral Development ◽

Frozen Storage ◽

High Quality ◽

Storage Duration ◽

Long Term Storage ◽

Term Storage ◽

Oriental Hybrid Lilies ◽

Scanning Electron

The effect of long-term storage of lily bulbs at -2 °C (frozen storage) and of high forcing temperatures on plant height and floral abnormalities was investigated with Oriental hybrid lilies from 1998 to 2000. `Acapulco' and `Simplon' bulbs were stored frozen at -2 °C for various lengths of time and were forced in fan- and pad-cooled greenhouses with temperatures ranging from 11 to 31 °C, depending on the season. The same cultivars were also forced in greenhouses and maintained year-round under refrigerated air conditioning with day/night temperatures of 16/15.5 °C or 18.5/18 °C. Floral development immediately after storage and at different intervals thereafter was observed by scanning electron microscopy (SEM). The prolonged frozen storage reduced the number of flowers. High greenhouse forcing temperatures during summer significantly accelerated flowering, resulted in short plants, and increased the number of abnormal flowers. Forcing at a low temperature (15.5 °C) after planting the frozen stored bulbs resulted in longer cut stems than those forced at 25 °C for 30 days after potting. Bulbs can be stored up to 9 months and still produce high-quality Oriental hybrid lilies.

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Tc and Re Behavior in Borosilicate Waste Glass Vapor Hydration Tests

MRS Proceedings ◽

10.1557/proc-985-0985-nn06-03 ◽

2006 ◽

Vol 985 ◽

Author(s):

David A. McKeown ◽

Andrew C. Buechele ◽

Wayne W. Lukens ◽

David K. Shuh ◽

Ian L. Pegg

Keyword(s):

High Mobility ◽

Sample Surface ◽

Hydrothermal Conditions ◽

X Ray ◽

Long Term Storage ◽

Before And After ◽

X Ray Absorption ◽

Term Storage ◽

Scanning Electron

AbstractTechnetium (Tc), found in some nuclear wastes, is of particular concern with regard to long-term storage, because of its long-lived radioactivity and high mobility in the environment. Tc and rhenium (Re), commonly used as a non-radioactive surrogate for Tc, were studied to assess their behavior in borosilicate glass under hydrothermal conditions in the Vapor Hydration Test (VHT). X-ray absorption spectroscopy (XAS) and scanning electron microscopy (SEM) measurements were made on the original Tc- and Re-containing glasses and their corresponding VHT samples, and show different behavior for Tc and Re under VHT conditions. XAS indicates that, despite starting with different Tc(IV) and Tc(VII) distributions in each glass, the VHT samples have 100% Tc(IV)O6 environments. SEM shows complete alteration of the original glass, Tc enrichment near the sample surface, and Tc depletion in the center. Perrhenate (Re(VII)O4−) is dominant in both Re-containing samples before and after the VHT, where Re is depleted near the VHT sample surface and more concentrated toward the center.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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