Effect of ipidacrine on acute pain and development of neuropathic pain syndrome in wistar rats

Цель исследования - изучение особенностей действия ипидакрина («Ипигрикс» Эйч Би Эм Фарма с.р.о., Словакия) на острую боль и динамику развития невропатического болевого синдрома у крыс линии Вистар. Методика. Проведено 2 серии опытов. В 1-й серии влияние ипидакрина изучали на моделях острой боли. Пороги болевой чувствительности определяли до внутримышечного введения ипидакрина (0,5 и 1 мг/кг), а также через 30 и 60 мин после введения. Оценку изменения индивидуальной болевой чувствительности у животных проводили с помощью 2 стандартных тестов: «Tail flick» и «Hot plate». Во 2-й серии изучали влияние ипидакрина на развитие невропатического болевого синдрома, который воспроизводили перерезкой опытным и контрольным животным левого седалищного нерва на уровне подколенной ямки. Интенсивность невропатического болевого синдрома у животных оценивали в баллах по выраженности аутотомии на оперированной конечности, времени появления аутотомий и количеству животных с аутотомией. Внутримышечное введение ипидакрина осуществляли ежедневно (1 мг/кг) в течение 20 сут. Результаты. Введение ипидакрина (0,5 мг/кг) не оказывает антиноцицептивного действия: порог болевой чувствительности не менялся по тестам «Tail flick» и «Hot рlate». Введение ипидакрина (1 мг/кг) за 30 мин до тестирования также не влияло на порог болевой чувствительности по тестам «Tail flick» и «Hot рlate», а через 60 мин после введения ипидакрина порог болевой чувствительности увеличился на 15,8 % (*р=0,033) по тесту «Tail flick» по сравнению с показателем до введения препарата. Заключение. Ипидакрин значительно задерживал развитие невропатического болевого синдрома НБС, но не оказывал влияния на острую боль в тестах «Tail flick» и «Hot plate». The aim of the study was to elucidate specific features of the effect of ipidacrine (IPIGRIKS, Slovakia) оn acute pain and development of neuropathic pain syndrome (NPS) in Wistar rats. Methods. Two series of experiments were performed. In the first series, effects of ipidacrine were studied on models of acute pain. Thresholds of pain sensitivity (PS) were determined before and 30 and 60 min after ipidacrine injection (0.5 and 1 mg/kg, i.m.). Changes in individual pain sensitivity were assessed with two standard tests, tail flick and hot plate. In the second series of experiments, the effect of ipidacrine on the development of NPS was studied. NPS was induced by transection of the left sciatic nerve at the level of popliteal fossa in experimental and control animals. The NPS intensity was evaluated in scores based on severity of autotomy on the operated extremity, time of autotomy emergence, and number of animals with autotomy. Ipidacrine (1 mg/kg, i.m.) was injected daily for 21 days. Results. Ipidacrine at a dose of 0.5 mg/kg did not exert an antinociceptive effect - the PS threshold did not change in the tail flick and hot plate tests. Ipidacrine at a dose of 1 mg/kg 30 min before testing did not affect the PS thresholds in the tail flick and hot plate tests; at 60 min after the ipidacrine injection, the PS thresholds increased by 15.8% (*p = 0.033) in the tail flick test compared to the value before the ipidacrine injection. Conclusion. Ipidacrine significantly delayed the development of NPS but did not affect acute pain in the tail flick and hot plate tests.

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Antinociceptive effects of intraperitoneal citicoline administration on rat models of acute pain

Nauchno-prakticheskii zhurnal «Patogenez» ◽

10.25557/2310-0435.2018.03.98-100 ◽

2018 ◽

pp. 98-100

Author(s):

Л.В. Кузнецова ◽

М.Н. Карпова ◽

Н.В. Клишина ◽

М.Л. Кукушкин

Keyword(s):

Acute Pain ◽

Wistar Rats ◽

Pain Sensitivity ◽

Antinociceptive Effect ◽

Tail Flick ◽

Hot Plate ◽

Antinociceptive Effects ◽

Series Of Experiments ◽

Dose Dependent ◽

Dose Dependent Effect

Цель исследования - изучение дозозависимого эффекта мультимодального препарата цитиколина на моделях острой боли у крыс линии Вистар. Методы. Оценку изменения индивидуальной болевой чувствительности у животных проводили с помощью 2 стандартных тестов: «Tail flick» и «Hot plate». Проведено 2 серии опытов. В 1-й и 2-й сериях опытов определяли пороги болевой чувствительности (ПБЧ) у животных до и через 1 час после внутрибрюшинного введения цитиколина в дозах 500 и 1000 мг/кг. Результаты. Введение цитиколин в дозе 500 мг/кг оказывает антиноцицептивное действие: ПБЧ повышался по тестам Hot рlate и Hot рlate. Увеличение дозы цитиколина до 1000 мг/кг не оказывало более выраженного антиноцицептивного действия. Заключение. Введение цитиколина оказывает антиноцицептивные эффекты у крыс, что может свидетельствовать о холинергической активации, индуцированной цитиколином. The aim was to study the dose-dependent effect of the multimodal drug citicoline on models of acute pain in Wistar rats. Methods. Assessment of changes in individual pain sensitivity was performed using 2 standard tests, Tail Flick and Hot Plate. Two series of experiments were carried out. In the 1st and 2nd series of experiments, pain thresholds (PS) were determined prior to and one hour after intraperitoneal citicoline administration at doses of 500 and 1000 mg/kg. Results. Administration of citicoline 500 mg/kg had an antinociceptive effect: PS increased both in the Tail Flick and in the Hot Plate tests. Increasing the citicoline dose to 1000 mg/kg did not exert a more pronounced antinociceptive effect. Conclusion. Citicoline exerts antinociceptive effects in rats, which may indicate the cholinergic activation induced by citicoline.

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Neuropathic pain syndrome in wistar rats at different stages of chronic brain epileptization (chemical kindling)

Russian Journal of Pain ◽

10.17116/pain20201803111 ◽

2020 ◽

Vol 18 (3) ◽

pp. 11

Author(s):

L.V. Kuznetsova ◽

M.N. Karpova ◽

N.Yu. Klishina ◽

M.L. Kukushkin

Keyword(s):

Neuropathic Pain ◽

Wistar Rats ◽

Pain Syndrome ◽

Neuropathic Pain Syndrome ◽

Chemical Kindling

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Faculty Opinions recommendation of Placebo response changes depending on the neuropathic pain syndrome: results of a systematic review and meta-analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717997054.793474154 ◽

2013 ◽

Author(s):

Nanna Finnerup

Keyword(s):

Systematic Review ◽

Neuropathic Pain ◽

Meta Analysis ◽

Placebo Response ◽

Pain Syndrome ◽

Neuropathic Pain Syndrome

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First experience with chronic epidural spinal cord stimulation in Khanty-Mansi autonomous okrug — clinical observations

Neurology Bulletin ◽

10.17816/nb70921 ◽

2021 ◽

Vol LIII (2) ◽

pp. 94-100

Author(s):

Olga A. Bondarenko ◽

Gaspar V. Gavrilov ◽

Vadim A. Padurets ◽

Roman V. Kasich

Keyword(s):

Spinal Cord ◽

Neuropathic Pain ◽

Spinal Cord Stimulation ◽

Pain Syndrome ◽

Chronic Neuropathic Pain ◽

Neuropathic Pain Syndrome ◽

Epidural Stimulation ◽

Cord Injury ◽

Epidural Spinal Cord Stimulation ◽

And Migration

Purpose of the work. The article is devoted to the first experience of epidural stimulation in the Khanty-Mansiysk Autonomous Okrug at the budgetary institution Surgut Clinical Trauma Hospital. Clinical examples are presented for two main indications for the application of this technique (disease of the operated spine, a consequence of spinal cord injury in combination with chronic neuropathic pain syndrome). Research methods. An assessment of the intensity of pain syndrome was given according to a visual analogue scale, the Pain Detect questionnaire; indicators of anxiety, depression on the HADS scale; quality of life according to the Oswestry questionnaire for a follow-up period of 6-12 months in patients with chronic epidural stimulation. Results. A positive assessment of the action during test neurostimulation was 63.3% (38 patients). Of the established permanent systems, a good result was achieved and persisted for 12 months or more in 96% (24 patients). It was necessary to change the stimulation parameters in 13% (3 patients). Revision of permanent systems was performed in 20% (5 patients), due to the progression of the degenerative-dystrophic process of the spine, damage and migration of system elements. Conclusions. Chronic epidural spinal cord stimulation has established itself as a personalized, highly effective, minimally invasive and safe method of treating chronic neuropathic pain syndromes. Multicomponent corrective action is of scientific interest and requires further study.

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Comparison of antinociceptive effect of the antiepileptic drug gabapentin to that of various dosage combinations of gabapentin with lamotrigine and topiramate in mice and rats

Journal of Neurosciences in Rural Practice ◽

10.4103/0976-3147.83577 ◽

2011 ◽

Vol 02 (02) ◽

pp. 130-136 ◽

Cited By ~ 9

Author(s):

Keshab Raj Paudel ◽

SK Bhattacharya ◽

GP Rauniar ◽

BP Das

Keyword(s):

Pain Perception ◽

Late Phase ◽

Antinociceptive Effect ◽

Experimental Pain ◽

Mechanical Hyperalgesia ◽

Tail Flick ◽

Hot Plate ◽

Analgesic Effects ◽

Pain Models ◽

Antinociceptive Effects

ABSTRACT Introduction: Newer anticonvulsants have a neuromodulatory effect on pain perception mechanisms in a hyperexcitable and damaged nervous system. Aim: This study was designed to study the analgesic effects of gabapentin alone and in combination with lamotrigine and topiramate in experimental pain models. Materials and Methods: Adult albino mice (n = 490) weighing 20–30 g and rats (n = 130) weighing 100–200 g were injected intraperitoneally with gabapentin, lamotrigine, and topiramate alone and in different dose combinations. The hot-plate method, tail-flick method, capsaicin-induced mechanical hyperalgesia, and formalin assay were used to assess the antinociceptive effects. Results: Of the three antiepileptic drugs, when given separately, gabapentin was more efficacious than either topiramate or lamotrigine in all the pain models. Combination of 25 mg/kg gabapentin with 25 mg/kg topiramate was more efficacious (P <.05) than 50 mg/kg gabapentin alone in the capsaicin-induced mechanical hyperalgesia test. Similarly, 50 mg/kg gabapentin with 50 mg/kg topiramate or 5 mg/kg lamotrigine was more efficacious (P <.05) than 50 or 100 mg/kg gabapentin alone in late-phase formalin-induced behaviors. Conclusions: Combination of gabapentin with either lamotrigine or topiramate produced better results than gabapentin alone in capsaicin-induced mechanical hyperalgesia test and in late-phase formalin-induced behaviors.

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A comparative study of antinociceptive effect of paroxetine with pethidine in acute pain in albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20172739 ◽

2017 ◽

Vol 6 (7) ◽

pp. 1728

Author(s):

Manju Gari ◽

Kumari Ranjeeta ◽

Lakhan Majhee ◽

Akhilesh Kumar ◽

Sumit Kumar Mahato

Keyword(s):

Acute Pain ◽

Water Immersion ◽

Antinociceptive Effect ◽

Warm Water ◽

Albino Rats ◽

Thermal Method ◽

Average Weight ◽

Tail Flick ◽

Immersion Method ◽

Standard Drug

Background: Pain is the most common reason patients seek medical care. Increased level of monoamines (serotonin and norepinephrine) in synaptic clefts lead to changes in pain threshold and induce antinociception. The study was carried out to evaluate antinociceptive effect of paroxetine in albino rats and to probe into its possible mechanism of action. The study was carried out to evaluate anti-nociceptive effect of paroxetine in albino rats.Methods: Male Albino rats of average weight 150-240gms were used. The drugs used were paroxetine 5mg/Kg, pethidine 5mg/kg (standard drug). Anti-nociceptive effect tested by using thermal method i.e. Tail flick response and Tail warm water immersion method.Results: In this study, Anti-nociceptive effect of respective drugs were measured by using two methods i.e. tail flick test and tail warm water immersion method at 0 min., 30 min., 60 min. and 90min.after administration of drugs. Reaction time started to increase from baseline at 0 min. and peak effect was seen at 60 min. then it started to decrease at 90 min. in almost all the groups except in control group.Conclusions: Paroxetine have significant analgesic effect in acute pain, which may be mediated via central and peripheral mechanisms. Efficacy of Paroxetine is almost equal to that of standard drug pethidine in acute pain management.

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Evaluation of analgesic activity of the methanol extract from the galls of Quercus infectoria alone and as an adjuvant in wistar rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20201521 ◽

2020 ◽

Vol 9 (5) ◽

pp. 701

Author(s):

Abishek P. Raichurkar ◽

Shashikala Gowdara Hanumanth Reddy

Keyword(s):

Analgesic Activity ◽

Wistar Rats ◽

Methanolic Extract ◽

Inflammatory Diseases ◽

Syringic Acid ◽

Tail Flick ◽

Hot Plate ◽

Anti Inflammatory ◽

Group 2 ◽

Quercus Infectoria

Background: Non-steroidal anti-inflammatory drugs and opioids are the most preferred drugs for pain relief. Considering the gastrointestinal toxicity, dependence and other side effects, search for better analgesic drug continues. Quercus infectoria (QI) is from the family Fagaceae. The galls of QI are comprised of tannin (36 to 60%), gallic acid, ellagic acid, and syringic acid. They possess antioxidant, anti-inflammatory, antimicrobial, and anti-diabetic properties. In India, galls of QI have been used for the treatment of toothache, diarrhoea, sore throat and inflammatory diseases as a home remedy. This study was conducted to evaluate the analgesic activity of methanolic extract of galls of QI on wistar rats using tail-flick and Eddy’s hot-plate methods. The objective of the study was to evaluate the analgesic activity of methanolic extract of galls of QI alone and as an adjuvant with tramadol on Wistar rats.Methods: Total of 24 wistar rats were included in the study and divided into 4 groups. They received drugs intra-peritoneally as follows. In group 1, normal saline, in group 2, tramadol, in group 3, methanolic extract of galls of QI and in group 4, tramadol with methanolic extract of galls of QI was available.Results: Methanolic extract of galls of QI produced significant maximal possible analgesia (<0.001) at 30 and 60 minutes in tail-flick method whereas it failed to produce analgesia in hot-plate method during all time intervals.Conclusions: Methanolic extract of galls of QI showed analgesic activity in tail-flick method indicating that its possible mechanism of action is spinally mediated.

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