Characterization of Plasma Proteins and Lipoproteins Using Microchannel Asymmetrical Flow Field-Flow Fractionation

2016 ◽  
Author(s):  
Soheyl Tadjiki
Keyword(s):  
Flow Field ◽  
Plasma Proteins ◽  
Field Flow Fractionation ◽  
Flow Fractionation

Characterization of non-solvent precipitated starch using asymmetrical flow field-flow fractionation coupled with multiple detectors

2019 ◽  
Vol 206 ◽  
pp. 21-28 ◽  
Author(s):  
Catalina Fuentes ◽  
Hisfazilah Saari ◽  
Jaeyeong Choi ◽  
Seungho Lee ◽  
Malin Sjöö ◽  
...  
Keyword(s):  
Flow Field ◽  
Field Flow Fractionation ◽  
Flow Fractionation ◽  
Multiple Detectors

Molar Mass Characterization of Crude Lignosulfonates by Asymmetric Flow Field-Flow Fractionation

2018 ◽  
Vol 7 (1) ◽  
pp. 216-223 ◽  
Author(s):  
Irina Sulaeva ◽  
Philipp Vejdovszky ◽  
Ute Henniges ◽  
Arnulf Kai Mahler ◽  
Thomas Rosenau ◽  
...  
Keyword(s):  
Flow Field ◽  
Molar Mass ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Flow Fractionation

Characterization of natural organic matter fractions separated by ultrafiltration using flow field-flow fractionation

2004 ◽  
Vol 38 (6) ◽  
pp. 1467-1476 ◽  
Author(s):  
Shoeleh Assemi ◽  
Gayle Newcombe ◽  
Chris Hepplewhite ◽  
Ronald Beckett
Keyword(s):  
Organic Matter ◽  
Flow Field ◽  
Natural Organic Matter ◽  
Field Flow Fractionation ◽  
Flow Fractionation ◽  
Organic Matter Fractions

scholarly journals Asymmetrical flow field-flow fractionation for improved characterization of human plasma lipoproteins

2018 ◽  
Vol 411 (3) ◽  
pp. 777-786 ◽  
Author(s):  
Carmen R. M. Bria ◽  
Farsad Afshinnia ◽  
Patrick W. Skelly ◽  
Thekkelnaycke M. Rajendiran ◽  
Pradeep Kayampilly ◽  
...  
Keyword(s):  
Flow Field ◽  
Human Plasma ◽  
Plasma Lipoproteins ◽  
Field Flow Fractionation ◽  
Flow Fractionation

scholarly journals Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4703
Author(s):  
Roland Drexel ◽  
Agnieszka Siupa ◽  
Pauline Carnell-Morris ◽  
Michele Carboni ◽  
Jo Sullivan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Field ◽  
Chemical Characterization ◽  
Rabbit Serum ◽  
Field Flow Fractionation ◽  
Nanoparticle Tracking Analysis ◽  
Biological Media ◽  
Physico Chemical ◽  
Flow Fractionation

Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix. An appropriate purification step can significantly reduce matrix interferences, and thus facilitate analysis of such demanding samples. Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) provides online sample purification while simultaneously enabling access to size and Zeta potential of sample constituents in the size range of approx. 1–1000 nm. Hyphenation of EAF4 with Multi-Angle Light Scattering (MALS) and Nanoparticle Tracking Analysis (NTA) detection adds high resolution size and number concentration information turning this setup into a powerful analytical platform for the comprehensive physico-chemical characterization of such challenging samples. We here present EAF4-MALS hyphenated with NTA for the analysis of liposomes and exosomes in complex, biological media. Coupling of the two systems was realized using a flow splitter to deliver the sample at an appropriate flow speed for the NTA measurement. After a proof-of-concept study using polystyrene nanoparticles, the combined setup was successfully applied to analyze liposomes and exosomes spiked into cell culture medium and rabbit serum, respectively. Obtained results highlight the benefits of the EAF4-MALS-NTA platform to study the behavior of these promising drug delivery vesicles under in vivo like conditions.

Characterization of size and aggregation for cellulose nanocrystal dispersions separated by asymmetrical-flow field-flow fractionation

Cellulose ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 2015-2028 ◽  
Author(s):  
Maohui Chen ◽  
Jeremie Parot ◽  
Arnab Mukherjee ◽  
Martin Couillard ◽  
Shan Zou ◽  
...  
Keyword(s):  
Flow Field ◽  
Cellulose Nanocrystal ◽  
Field Flow Fractionation ◽  
Flow Fractionation
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