Effect of Bovine Serum Albumin on Gelatin-Oleic Nanoparticles on Physicochemical Properties and Cellular Uptake

2016 ◽  
Author(s):  
Beom-Jin Lee
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Bovine Serum

N-acetylcysteine delivery with silica nanoparticles into 3T3-L1 adipocytes

2021 ◽  
Author(s):  
Evelyn Frontera ◽  
Martin F Desimone ◽  
Mauricio C De Marzi ◽  
Liliana N Guerra
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Cellular Uptake ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cellular Accumulation ◽  
Dose Dependent

Background: The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials & methods: Silica NPs, 20 ± 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin. Results: While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.

scholarly journals Metabolism of platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-PAF (1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) by cultured rat Kupffer cells

1989 ◽  
Vol 261 (1) ◽  
pp. 77-81 ◽  
Author(s):  
W Chao ◽  
A Siafaka-Kapadai ◽  
D J Hanahan ◽  
M S Olson
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cellular Uptake ◽  
Kupffer Cells ◽  
Bovine Serum ◽  
Platelet Activating Factor ◽  
Metabolic Product ◽  
Supernatant Fraction ◽  
Substantial Influence ◽  
Subsequent Formation

The metabolism of platelet-activating factor (PAF; identified as AGEPC: 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-PAF (lyso-GEPC: 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) was investigated in cultured rat Kupffer cells. The rat Kupffer cells accumulated [3H]AGEPC and deacetylated this compound to the corresponding [3H]lyso-GEPC, which was the major metabolic product of [3H]AGEPC. [3H]Lyso-GEPC was distributed primarily in the supernatant fraction of incubated cells throughout the experimental interval. Only a very small portion of the [3H]lyso-GEPC was further converted to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (alkylacyl-GPC), indicating that this acylation process was not particularly active in these cells. When [3H]lyso-GEPC was incubated with Kupffer cells, the conversion of lyso-GEPC to AGEPC via the acetyltransferase reaction increased up to 30 min and declined thereafter. Bovine serum albumin (BSA) had a substantial influence on both the cellular uptake and the metabolism of [3H]AGEPC. An increase in the BSA concentration in the incubation media reduced the cellular uptake of [3H]AGEPC and the subsequent formation of lyso-GEPC. The results of this study suggest that the hepatic Kupffer cells play an important role in the metabolism of PAF. Moreover, these results infer that the regulation of the PAF level in certain hepatic pathophysiological situations may be a consequence of the production and subsequent metabolism of this potent lipid autacoid in the Kupffer cells of the liver.

Improving the physicochemical properties of bicalutamide by complex formation with bovine serum albumin

2017 ◽  
Vol 106 ◽  
pp. 381-392 ◽  
Author(s):  
Congbin Yang ◽  
Peiwen Di ◽  
JinPing Fu ◽  
Hui Xiong ◽  
Qiufang Jing ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Physicochemical Properties ◽  
Bovine Serum
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