scholarly journals APLIKASI BIOSURFAKTAN Bacillus subtilis ATCC 19659 DENGAN MEDIA PRODUKSI LIMBAH TAHU UNTUK ENHACED OIL RECOVERY

2021 ◽  
Vol 9 (2) ◽  
pp. 101
Author(s):  
Rizqy Fachria
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Liquid Waste ◽  
Nutrient Broth ◽  
Microbial Enhanced Oil Recovery ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Biosurfactant as secondary metabolit produced by Bacillus subtilis. It has the ability to emulsify and reduce the surface tension. Biosurfactants produced by B. subtilis is a lipopeptide. Furthermore, biosurfactant can be utilized in microbial enhanced oil recovery (MEOR). In this research, biosurfactant of B. subtilis ATCC 19 659 were evaluated. The production use Nutrient Broth (NB) and soybean liquid waste. Application of biosurfactant in oil recovery showed that biosurfactant of NB recover 2 mL crude oil and biosurfactant of soybean liquid waste medium recover 3.67 mL.

Application of Bacillus subtilis strain for microbial-enhanced oil recovery

2019 ◽  
Vol 16 (7) ◽  
pp. 530-539 ◽  
Author(s):  
Haiyan Xu ◽  
Huanjiang Wang ◽  
Weihong Jia ◽  
Sili Ren ◽  
Jinqing Wang
Keyword(s):  
Bacillus Subtilis ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Subtilis Strain ◽  
Microbial Enhanced Oil Recovery

A novel sophorolipid-producing Candida keroseneae GBME-IAUF-2 as a potential agent in microbial enhanced oil recovery (MEOR)

2020 ◽  
Vol 367 (17) ◽  
Author(s):  
Zahra Ganji ◽  
Keivan Beheshti-Maal ◽  
Ahmadreza Massah ◽  
Zarrindokht Emami-Karvani
Keyword(s):  
Surface Tension ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Microbial Enhanced Oil Recovery ◽  
Original Activity ◽  
5.8S Rdna ◽  
Food Industries ◽  
Emulsification Index ◽  
Isolation And Characterization ◽  
Displacement Assay

ABSTRACT The biosurfactants have extensive applications in food and petroleum microbiology. The aims of this research were isolation and characterization of thermo-tolerant biosurfactants from highly producing yeast strains. The Bushnell Hass medium was used for screening the biosurfactant-producing yeasts. Biosurfactant presence was evaluated using oil displacement assay and surface tension test. The best biosurfactant-producing strain was named Candida keroseneae GBME-IAUF-2 and its 5.8s-rDNA sequence was deposited in GenBank, NCBI, under the accession number MT012957.1. The thin layer chromatography and Fourier-transform infrared spectroscopy analysis confirmed that the extracted biosurfactant was sophorolipid with a significant surface activity. The purified sophorolipid decreased the surface tension of water from 72 to 29.1 mN/m. Its maximum emulsification index, E24%, was recorded as 60% and preserved 92.06–97.25% of its original activity at 110–120°C. It also preserved 89.11% and 84.73% of its original activity in pH of 9.3 and 10.5, respectively. It preserved 96.66–100% of its original activity in saline extreme conditions. This is the first report of sophorolipid production by the yeast C. keroseneae. According to the high thermal, pH and saline stability, the sophorolipid produced by C. keroseneae GBME-IAUF-2 could be highly recommended for applications in microbial enhanced oil recovery as well as food industries as an excellent emulsifying agent.

scholarly journals Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications

Fuel ◽  
2013 ◽  
Vol 111 ◽  
pp. 259-268 ◽  
Author(s):  
Jorge F.B. Pereira ◽  
Eduardo J. Gudiña ◽  
Rita Costa ◽  
Rui Vitorino ◽  
José A. Teixeira ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Biosurfactant Production ◽  
Microbial Enhanced Oil Recovery

scholarly journals Development of Coupled Biokinetic and Thermal Model to Optimize Cold-Water Microbial Enhanced Oil Recovery (MEOR) in Homogenous Reservoir

2019 ◽  
Vol 11 (6) ◽  
pp. 1652 ◽  
Author(s):  
Eunji Hong ◽  
Moon Jeong ◽  
Tae Kim ◽  
Ji Lee ◽  
Jin Cho ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Interfacial Tension ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Thermal Model ◽  
Cold Water ◽  
Injection Rate ◽  
Experimental Results ◽  
Microbial Enhanced Oil Recovery ◽  
Optimal Injection

By incorporating a temperature-dependent biokinetic and thermal model, the novel method, cold-water microbial enhanced oil recovery (MEOR), was developed under nonisothermal conditions. The suggested model characterized the growth for Bacillus subtilis (microbe) and Surfactin (biosurfactant) that were calibrated and confirmed against the experimental results. Several biokinetic parameters were obtained within approximately a 2% error using the cardinal temperature model and experimental results. According to the obtained parameters, the examination was conducted with several injection scenarios for a high-temperature reservoir of 71 °C. The results proposed the influences of injection factors including nutrient concentration, rate, and temperature. Higher nutrient concentrations resulted in decreased interfacial tension by producing Surfactin. On the other hand, injection rate and temperature changed growth condition for Bacillus subtilis. An optimal value of injection rate suggested that it affected not only heat transfer but also nutrient residence time. Injection temperature led to optimum reservoir condition for Surfactin production, thereby reducing interfacial tension. Through the optimization process, the determined optimal injection design improved oil recovery up to 53% which is 8% higher than waterflooding. The proposed optimal injection design was an injection sucrose concentration of 100 g/L, a rate of 7 m3/d, and a temperature of 19 °C.

Investigation of Indigenous Microbial Enhanced Oil Recovery in a Middle Salinity Petroleum Reservoir

2011 ◽  
Vol 365 ◽  
pp. 326-331 ◽  
Author(s):  
Yue Hui She ◽  
Fu Chang Shu ◽  
Zheng Liang Wang ◽  
Long Jiang Yu
Keyword(s):  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Freezing Point ◽  
Most Probable Number ◽  
Petroleum Reservoir ◽  
Microbial Enhanced Oil Recovery ◽  
Core Flooding ◽  
Probable Number ◽  
The Core

Culture-based techniques were applied to analyze the diversity of indigenous microbial communities in the Qinghai middle salinity petroleum reservoir (QH-MSPR). The results of the most probable number (MPN) method indicated there was an abundance of indigenous microbes (105-106MPN/ml). Two isolations (BIOS682) from the QH-MSPR were identified as Brevibacillus agri and Brevibacillus levickii. The study showed that BIOS682 enhanced the degradation rate of Huatugou crude oil. The viscosity and freezing point of crude oil after treatment by BIOS682 were all decreased. The results of TLC and FTIR spectrum analysis of the biosurfactant produced by BIOS682 indicated that it was identical to that of lipopeptide. The core-flooding tests showed that the incremental oil recoveries were 7.05-10.15%. Thus, BIOS682 may provide a viable application of microbial enhanced oil recovery (MEOR).

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