Investigation of Indigenous Microbial Enhanced Oil Recovery in a Middle Salinity Petroleum Reservoir

2011 ◽  
Vol 365 ◽  
pp. 326-331 ◽  
Author(s):  
Yue Hui She ◽  
Fu Chang Shu ◽  
Zheng Liang Wang ◽  
Long Jiang Yu
Keyword(s):  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Freezing Point ◽  
Most Probable Number ◽  
Petroleum Reservoir ◽  
Microbial Enhanced Oil Recovery ◽  
Core Flooding ◽  
Probable Number ◽  
The Core

Culture-based techniques were applied to analyze the diversity of indigenous microbial communities in the Qinghai middle salinity petroleum reservoir (QH-MSPR). The results of the most probable number (MPN) method indicated there was an abundance of indigenous microbes (105-106MPN/ml). Two isolations (BIOS682) from the QH-MSPR were identified as Brevibacillus agri and Brevibacillus levickii. The study showed that BIOS682 enhanced the degradation rate of Huatugou crude oil. The viscosity and freezing point of crude oil after treatment by BIOS682 were all decreased. The results of TLC and FTIR spectrum analysis of the biosurfactant produced by BIOS682 indicated that it was identical to that of lipopeptide. The core-flooding tests showed that the incremental oil recoveries were 7.05-10.15%. Thus, BIOS682 may provide a viable application of microbial enhanced oil recovery (MEOR).

Characteristics of a Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 542-545
Author(s):  
Xiang Ping Kong
Keyword(s):  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Sewage Treatment ◽  
Core Flooding ◽  
Treatment Technology ◽  
Promising Candidate ◽  
Different Types ◽  
Displacement Experiments ◽  
Original Oil In Place

The enhanced oil recovery characteristics of a Geobacillus sp. was investigated by shake flask experiments, blind-tube oil displacement experiments and core flooding tests. The strain exhibited good properties such as resisting high temperature, taking different types of crude oil as the sole carbon source, reducing the viscosity of crude oil, emulsifying and dispersing crude oil or liquid wax. The oil in the dead area could be effectively driven out by the strain, and the oil recovery of original oil in place had been increased by 12.9-15.9% after 5 treatments in 50 days by adopting air-assistant technique (air/liquid 10:1, v/v) due to the synergistic effect of the bacteria and their metabolites such as biogas and biosurfactants. The strain seems to be a promising candidate for microbial enhanced oil recovery and underground sewage treatment technology.

The field pilot of microbial enhanced oil recovery in a high temperature petroleum reservoir

2005 ◽  
Vol 48 (3-4) ◽  
pp. 265-271 ◽  
Author(s):  
Liu Jinfeng ◽  
Ma Lijun ◽  
Mu Bozhong ◽  
Liu Rulin ◽  
Ni Fangtian ◽  
...  
Keyword(s):  
High Temperature ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Petroleum Reservoir ◽  
Microbial Enhanced Oil Recovery

scholarly journals APLIKASI BIOSURFAKTAN Bacillus subtilis ATCC 19659 DENGAN MEDIA PRODUKSI LIMBAH TAHU UNTUK ENHACED OIL RECOVERY

2021 ◽  
Vol 9 (2) ◽  
pp. 101
Author(s):  
Rizqy Fachria
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Liquid Waste ◽  
Nutrient Broth ◽  
Microbial Enhanced Oil Recovery ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Biosurfactant as secondary metabolit produced by Bacillus subtilis. It has the ability to emulsify and reduce the surface tension. Biosurfactants produced by B. subtilis is a lipopeptide. Furthermore, biosurfactant can be utilized in microbial enhanced oil recovery (MEOR). In this research, biosurfactant of B. subtilis ATCC 19 659 were evaluated. The production use Nutrient Broth (NB) and soybean liquid waste. Application of biosurfactant in oil recovery showed that biosurfactant of NB recover 2 mL crude oil and biosurfactant of soybean liquid waste medium recover 3.67 mL.

scholarly journals Stimulation of indigenous microbes by optimizing the water cut in low permeability reservoirs for green and enhanced oil recovery

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kai Cui ◽  
Zhiyong Zhang ◽  
Zhongzhi Zhang ◽  
Shanshan Sun ◽  
Hailan Li ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Low Permeability ◽  
Petroleum Reservoir ◽  
Activation Time ◽  
Water Cut ◽  
Microbial Groups ◽  
Low Permeability Reservoirs ◽  
Stimulation Of

Abstract Low permeability oil reservoirs are a widespread petroleum reservoir type all over the world. Therefore, methods to recover these reservoirs efficiently are of importance to guarantee energy supply. Here we report our novel stimulation of indigenous microbes by optimizing the water cut in low permeability reservoirs for green and enhanced oil recovery. We aimed to investigate the characteristics of indigenous bacterial communities with changes in water cut in reservoirs by high-throughput sequencing technology, and reveal the mechanism and characteristics of the crude oil biotreatment under different crude oil-water ratio conditions and the optimum activation time of indigenous functional microbial groups in reservoirs. The indigenous microbial metabolism products were characterized by gas chromatography mass spectrometry. Results showed that Acinetobacter (47.1%) and Pseudomones (19.8%) were the main functional genus of crude oil degradation at the optimal activation time, and can reduce the viscosity of crude oil from 8.33 to 5.75 mPa·s. The dominant bacteria genus for oil recovery after activation of the production fluids was similar to those in the reservoirs with water cut of 60–80%. Furthermore seven mechanism pathways of enhancing oil recovery by the synergistic of functional microbial groups and their metabolites under different water cut conditions in low permeability reservoirs have been established.

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