scholarly journals Taurine is Covalently Incorporated into Alpha Tubulin

2019 ◽  
Author(s):  
Matthew T Olson ◽  
Alfred L Yergey ◽  
Kamalika Mukherjee ◽  
melissa Pergande ◽  
susan Bane ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Free Amino ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Carboxyl Terminus ◽  
Alpha Tubulin ◽  
Avian Erythrocytes ◽  
First Time ◽  
Resolution Mass

We use various methods of high resolution mass spectrometry to demonstrate that taurine is covalently attached to the carboxyl terminus of alpha Tubulin from avian erythrocytes. The alpha Tubulin terminal residue, a tyrosine, must already be removed before covalent addition of taurine, via a known cycle of de-tyrosination and re-tyrosination. Taurination appears to stop this cycle. Taurine is the most abundant free amino acid in the body but this is the first time that it has been shown to be covalently incorporated into any protein.

scholarly journals Taurine is Covalently Incorporated into Alpha Tubulin

2019 ◽  
Author(s):  
Matthew T Olson ◽  
Alfred L Yergey ◽  
Kamalika Mukherjee ◽  
melissa Pergande ◽  
susan Bane ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Free Amino ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Carboxyl Terminus ◽  
Alpha Tubulin ◽  
Avian Erythrocytes ◽  
First Time ◽  
Resolution Mass

We use various methods of high resolution mass spectrometry to demonstrate that taurine is covalently attached to the carboxyl terminus of alpha Tubulin from avian erythrocytes. The alpha Tubulin terminal residue, a tyrosine, must already be removed before covalent addition of taurine, via a known cycle of de-tyrosination and re-tyrosination. Taurination appears to stop this cycle. Taurine is the most abundant free amino acid in the body but this is the first time that it has been shown to be covalently incorporated into any protein.

Measuring Exposome Associated with Health

2020 ◽  
pp. 46-50
Keyword(s):  
Mass Spectrometry ◽  
Remote Sensing ◽  
High Resolution ◽  
Biological Effect ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Ecological Environment ◽  
Special Issue ◽  
The Life Course ◽  
Resolution Mass

The concept of exposome has received increasing discussion, including the recent Special Issue of Science –"Chemistry for Tomorrow's Earth,” about the feasibility of using high-resolution mass spectrometry to measure exposome in the body, and tracking the chemicals in the environment and assess their biological effect. We discuss the challenges of measuring and interpreting the exposome and suggest the survey on the life course history, built and ecological environment to characterize the sample of study, and in combination with remote sensing. They should be part of exposomics and provide insights into the study of exposome and health.

scholarly journals Alkaloids from Papaver coreanum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601
Author(s):  
Dong-Ung Lee ◽  
Jong Hee Park ◽  
Ludger Wessjohann ◽  
Jürgen Schmidt
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Endemic Plant ◽  
High Resolution Mass ◽  
Main Components ◽  
First Time ◽  
Resolution Mass

The alkaloid pattern of the endemic plant Papaver coreanum Nakai (Papaveraceae) was determined for the first time. Eight alkaloids could be identified by LC/ESIMS/MS and high-resolution mass spectrometry. Among them, protopine and allocryptopine represent the main components. Besides norsanguinarine, sanguinarine, dihydrosanguinarine, oxysanguinarine, lincangenine, and cryptopine, some other trace alkaloids were found whose structures remain unknown.

scholarly journals Hypochlorite-induced damage of plasminogen molecules: structural-functional disturbance

2019 ◽  
Vol 488 (5) ◽  
pp. 560-566
Author(s):  
A. D. Vasilyeva ◽  
L. V. Yurina ◽  
A. N. Shchegolikhin ◽  
A. E. Bugrova ◽  
T. S. Konstantinova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Structure ◽  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Data ◽  
High Tolerance ◽  
Functional Disturbance ◽  
Closed Conformation ◽  
First Time ◽  
Resolution Mass

Plasminogen being a precursor of plasmin, a serine protease which plays a fundamental role in the intravascular thrombolysis. For the first time, by using high-resolution mass spectrometry, data were obtained of oxidative modifications of the plasminogen molecule under induced oxidation. The FTIR data show that under oxidation on the protein, its secondary structure also undergoes the rearrangements. The high tolerance of plasminogen to oxidation can be due to both the closed conformation and the ability of some Met residues to serve as ROS trap.

scholarly journals New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 864
Author(s):  
Marina Astudillo-Pascual ◽  
Irene Domínguez ◽  
Pedro A. Aguilera ◽  
Antonia Garrido Frenich
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Compounds ◽  
High Resolution ◽  
Detection Method ◽  
High Resolution Mass Spectrometry ◽  
Posidonia Oceanica ◽  
High Resolution Mass ◽  
First Time ◽  
Different Tissues ◽  
Resolution Mass

The studies on the Posidonia oceanica Delile (P. oceanica) phenolic composition have been focused on the foliar tissues and have often neglected the phenolic compounds in rhizomes or roots alike. With the current improvements in high resolution mass spectrometry (HRMS) analyzers, such as the Orbitrap MS, there is a new opportunity to more deeply study P. oceanica. One of the benefits is the possibility of conducting an exhaustive phenolic monitoring, which is crucial in the search for new stressor-specific biomarkers of coastal deterioration. For this purpose, the different tissues (leaf, rhizome, and root) of P. oceanica seagrass from several marine sampling areas were analyzed through target, suspected, and non-target screenings. This paper brings a fast and tissues-specific extraction, as well as a detection method of phenolic compounds applying for the first time the potential of HRMS (Exactive Orbitrap) in P. oceanica samples. As a result, 42 phenolic compounds were satisfactorily detected, of which, to our knowledge, 24 were not previously reported in P. oceanica, such as naringenin, naringenin chalcone and pinocembrin, among others. Information here reported could be used for the evaluation of new stressor-specific biomarkers of coastal deterioration in the Mediterranean waters. Furthermore, the followed extraction and analytical method could be considered as a reference protocol in other studies on marine seagrasses due to the exhaustive search and satisfactory results.

scholarly journals Liquid chromatography – high resolution mass spectrometry screening of Astragalus hamosus and Astragalus corniculatus

Pharmacia ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 135-139
Author(s):  
Aleksandar Shkondrov ◽  
Ilina Krasteva
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Ionisation Mass Spectrometry ◽  
First Time ◽  
Ionisation Mass ◽  
Resolution Mass

Astragalus hamosus and Astragalus corniculatus were examined for the presence of flavoalkaloids, acylated and highly glycosylated flavonoids. Non-purified extracts of the overground parts of the species were subjected to ultra-high performance liquid chromatography – high resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) analysis and the results were compared to authentic reference substances. A flavoalkaloid of kaempferol was newly identified in an extract of A. hamosus. In addition, three compounds – quercetin and kaempferol flavonoids, acylated with hydroxymethylglutaric acid and alcesefoliside, were found in extracts of A. hamosus and A. corniculatus for the first time.

Exploring the Active Compounds of Traditional Mongolian Medicine Agsirga in Intervention of Novel Coronavirus (2019-nCoV) Based on HPLC-Q-Exactive-MS/MS and Molecular Docking Method

2020 ◽  
Author(s):  
Jie Cheng ◽  
Yuchen Tang ◽  
Baoquan Bao ◽  
Ping Zhang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
High Resolution ◽  
Mass Spectra ◽  
High Resolution Mass Spectrometry ◽  
Structural Formula ◽  
Active Compounds ◽  
High Resolution Mass ◽  
Q Exactive ◽  
Resolution Mass

<p><a></a><a></a><a></a><a><b>Objective</b></a>: To screen all compounds of Agsirga based on the HPLC-Q-Exactive high-resolution mass spectrometry and find potential inhibitors that can respond to 2019-nCoV from active compounds of Agsirga by molecular docking technology.</p> <p><b>Methods</b>: HPLC-Q-Exactive high-resolution mass spectrometry was adopted to identify the complex components of Mongolian medicine Agsirga, and separated by the high-resolution mass spectrometry Q-Exactive detector. Then the Orbitrap detector was used in tandem high-resolution mass spectrometry, and the related molecular and structural formula were found by using the chemsipider database and related literature, combined with precise molecular formulas (errors ≤ 5 × 10<sup>−6</sup>) , retention time, primary mass spectra, and secondary mass spectra information, The fragmentation regularities of mass spectra of these compounds were deduced. Taking ACE2 as the receptor and deduced compounds as the ligand, all of them were pretreated by discover studio, autodock and Chem3D. The molecular docking between the active ingredients and the target protein was studied by using AutoDock molecular docking software. The interaction between ligand and receptor is applied to provide a choice for screening anti-2019-nCoV drugs.</p> <p><b>Result</b>: Based on the fragmentation patterns of the reference compounds and consulting literature, a total of 96 major alkaloids and stilbenes were screened and identified in Agsirga by the HPLC-Q-Exactive-MS/MS method. Combining with molecular docking, a conclusion was got that there are potential active substances in Mongolian medicine Agsirga which can block the binding of ACE2 and 2019-nCoV at the molecular level.</p>

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