Affinity Liquid Chromatography Method for the Quantification of Immunoglobulin G in Bovine Colostrum Powders
2006 ◽
Vol 89
(5)
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pp. 1249-1256
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Keyword(s):
Abstract An affinity liquid chromatography (LC) method for the determination of bovine immunoglobulin G (IgG), using protein G coupled to an agarose support, was modified to permit the quantification of IgG in colostrum-based powders. Sample preparation included pH adjustment to 4.6 to precipitate casein and denatured whey protein. The method was applied to a range of colostrum powders and was compared with the alternative independent methods of surface plasmon resonance immunoassay, radial immunodiffusion, and reversed-phase LC. The method was rapid, and performance parameters included a working range of 10-150 μg IgG and precision relative standard deviation values of <10%.
2014 ◽
Vol 1353
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pp. 28-39
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2013 ◽
Vol 9
(2)
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pp. 26-29
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1995 ◽
Vol 692
(1-2)
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pp. 121-129
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1999 ◽
Vol 21
(6)
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pp. 609
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2008 ◽
Vol 1185
(2)
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pp. 251-257
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