Ion-Mobility Mass Spectrometry of Glycans and Glycopeptides Using Both Intact and Glycan Sequencing Approaches In Positive Ion Mode Facilitates Glycan Isomer and Glycan Motif Identification
Keyword(s):
High resolution glycan analysis has become an important part of biopharmaceutical API production and quality control. Liquid chromatography (LC) is now a well-established technique in this field but the resolution of similar isomeric glycan structures is still a challenge. Here we show that the addition of ion mobility spectroscopy (IMS) in a hyphenated LC-IMS-MS setting allows for the high resolution of N-glycan isomers during positive ion analysis. We have identified unique features in the IM chromatograms to help differentiate a range of isomeric N-glycans for both RFMS labelled glycans and glycopeptides.<br>
2020 ◽
2016 ◽
Vol 407
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pp. 69-76
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Keyword(s):
2016 ◽
Vol 1860
(8)
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pp. 1688-1709
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Keyword(s):
2017 ◽
Vol 412
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pp. 14-19
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Keyword(s):
2013 ◽
2017 ◽
Vol 423
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pp. 54-58
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2016 ◽
Vol 402
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pp. 1-11
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