scholarly journals The Steadfast Au@Pt Soldier: Peroxide-Tolerant Nanozyme for Signal Enhancement in Lateral Flow Immunoassay of Peroxidase-Containing Samples

2020 ◽  
Author(s):  
Vasily Panferov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Hydrogen Peroxide ◽  
Potato Virus ◽  
Limit Of Detection ◽  
Potato Virus X ◽  
Fundamental Principle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

<div>The approach to inhibit endogenous peroxidases by elevated concentrations of hydrogen peroxide while maintaining the high peroxidase-mimicking activity of Au@Pt nanozymes was developed. The approach facilitates selective and highly-sensitive detection of peroxidase-mimicking nanozyme nanozymes in the background of endogenous peroxidases. Au@Pt nanozyme was used as the colorimetric and catalytic label in lateral flow immunoassay of an important plant pathogen – potato virus X. The inhibition of endogenous peroxidases in plant extracts and selective detection of Au@Pt nanozyme provides the lowest limit of detection among immunochemical assays of potato virus X (up to 500 times lower compared to the assay with conventional gold nanoparticles). </div><div>The proposed approach uses the fundamental principle of enzyme inhibition by the substrate. It is universal and applicable to all matrixes with peroxidase activity. </div>

scholarly journals The Steadfast Au@Pt Soldier: Peroxide-Tolerant Nanozyme for Signal Enhancement in Lateral Flow Immunoassay of Peroxidase-Containing Samples

2020 ◽  
Author(s):  
Vasily Panferov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Hydrogen Peroxide ◽  
Potato Virus ◽  
Limit Of Detection ◽  
Potato Virus X ◽  
Fundamental Principle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

<div>The approach to inhibit endogenous peroxidases by elevated concentrations of hydrogen peroxide while maintaining the high peroxidase-mimicking activity of Au@Pt nanozymes was developed. The approach facilitates selective and highly-sensitive detection of peroxidase-mimicking nanozyme nanozymes in the background of endogenous peroxidases. Au@Pt nanozyme was used as the colorimetric and catalytic label in lateral flow immunoassay of an important plant pathogen – potato virus X. The inhibition of endogenous peroxidases in plant extracts and selective detection of Au@Pt nanozyme provides the lowest limit of detection among immunochemical assays of potato virus X (up to 500 times lower compared to the assay with conventional gold nanoparticles). </div><div>The proposed approach uses the fundamental principle of enzyme inhibition by the substrate. It is universal and applicable to all matrixes with peroxidase activity. </div>

Enhancement of lateral flow immunoassay by alkaline phosphatase: a simple and highly sensitive test for potato virus X

2017 ◽  
Vol 185 (1) ◽  
Author(s):  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Yury A. Varitsev ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Alkaline Phosphatase ◽  
Potato Virus ◽  
Potato Virus X ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Sensitive Test ◽  
Highly Sensitive

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

Double-enhanced lateral flow immunoassay for potato virus X based on a combination of magnetic and gold nanoparticles

2018 ◽  
Vol 1007 ◽  
pp. 50-60 ◽  
Author(s):  
Shyatesa C. Razo ◽  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Yuri A. Varitsev ◽  
Anatoly V. Zherdev ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Potato Virus ◽  
Potato Virus X ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals An Ultrasensitive Silicon-Based Electrolyte-Gated Transistor for the Detection of Peanut Allergens

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 24
Author(s):  
Donghoon Kim ◽  
Bo Jin ◽  
Sol-A Kim ◽  
Wonyeong Choi ◽  
Seonghwan Shin ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
High Sensitivity ◽  
Drain Current ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Peanut Allergens ◽  
Low Threshold ◽  
Highly Sensitive Detection ◽  
Silicon Based ◽  
Specific Control

The highly sensitive detection of peanut allergens (PAs) using silicon-based electrolyte-gated transistors (Si-EGTs) was demonstrated. The Si-EGT was made using a top-down technique. The fabricated Si-EGT showed excellent intrinsic electrical characteristics, including a low threshold voltage of 0.7 V, low subthreshold swing of <70 mV/dec, and low gate leakage of <10 pA. Surface functionalization and immobilization of antibodies were performed for the selective detection of PAs. The voltage-related sensitivity (SV) showed a constant behavior from the subthreshold regime to the linear regime. The current-related sensitivity (SI) was high in the subthreshold regime and then significantly decreased as the drain current increased. The limit of detection (LOD) was calculated to be as low as 25 pg/mL based on SI characteristics, which is the lowest value reported to date in the literature for various sensor methodologies. The Si-EGT showed selective detection of PA through a non-specific control test. These results confirm that Si-EGT is a high-sensitivity and low-power biosensor for PA detection.

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