scholarly journals An Ultrasensitive Silicon-Based Electrolyte-Gated Transistor for the Detection of Peanut Allergens

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 24
Author(s):  
Donghoon Kim ◽  
Bo Jin ◽  
Sol-A Kim ◽  
Wonyeong Choi ◽  
Seonghwan Shin ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
High Sensitivity ◽  
Drain Current ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Peanut Allergens ◽  
Low Threshold ◽  
Highly Sensitive Detection ◽  
Silicon Based ◽  
Specific Control

The highly sensitive detection of peanut allergens (PAs) using silicon-based electrolyte-gated transistors (Si-EGTs) was demonstrated. The Si-EGT was made using a top-down technique. The fabricated Si-EGT showed excellent intrinsic electrical characteristics, including a low threshold voltage of 0.7 V, low subthreshold swing of <70 mV/dec, and low gate leakage of <10 pA. Surface functionalization and immobilization of antibodies were performed for the selective detection of PAs. The voltage-related sensitivity (SV) showed a constant behavior from the subthreshold regime to the linear regime. The current-related sensitivity (SI) was high in the subthreshold regime and then significantly decreased as the drain current increased. The limit of detection (LOD) was calculated to be as low as 25 pg/mL based on SI characteristics, which is the lowest value reported to date in the literature for various sensor methodologies. The Si-EGT showed selective detection of PA through a non-specific control test. These results confirm that Si-EGT is a high-sensitivity and low-power biosensor for PA detection.

scholarly journals The Steadfast Au@Pt Soldier: Peroxide-Tolerant Nanozyme for Signal Enhancement in Lateral Flow Immunoassay of Peroxidase-Containing Samples

2020 ◽  
Author(s):  
Vasily Panferov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Hydrogen Peroxide ◽  
Potato Virus ◽  
Limit Of Detection ◽  
Potato Virus X ◽  
Fundamental Principle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

<div>The approach to inhibit endogenous peroxidases by elevated concentrations of hydrogen peroxide while maintaining the high peroxidase-mimicking activity of Au@Pt nanozymes was developed. The approach facilitates selective and highly-sensitive detection of peroxidase-mimicking nanozyme nanozymes in the background of endogenous peroxidases. Au@Pt nanozyme was used as the colorimetric and catalytic label in lateral flow immunoassay of an important plant pathogen – potato virus X. The inhibition of endogenous peroxidases in plant extracts and selective detection of Au@Pt nanozyme provides the lowest limit of detection among immunochemical assays of potato virus X (up to 500 times lower compared to the assay with conventional gold nanoparticles). </div><div>The proposed approach uses the fundamental principle of enzyme inhibition by the substrate. It is universal and applicable to all matrixes with peroxidase activity. </div>

Highly sensitive detection of nitrite by using gold nanoparticle-decorated α-Fe2O3 nanorod arrays as self-supporting photo-electrodes

2019 ◽  
Vol 6 (6) ◽  
pp. 1432-1441 ◽  
Author(s):  
Shenghong Kang ◽  
Haimin Zhang ◽  
Guozhong Wang ◽  
Yunxia Zhang ◽  
Huijun Zhao ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Nanorod Arrays ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gold nanoparticle (Au NP)-decorated-Fe2O3 nanorod arrays (AuNPs-Fe2O3) as a photoelectrode are applied to the detection of nitrite solution with a low limit of detection and high sensitivity.

scholarly journals The Steadfast Au@Pt Soldier: Peroxide-Tolerant Nanozyme for Signal Enhancement in Lateral Flow Immunoassay of Peroxidase-Containing Samples

2020 ◽  
Author(s):  
Vasily Panferov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Hydrogen Peroxide ◽  
Potato Virus ◽  
Limit Of Detection ◽  
Potato Virus X ◽  
Fundamental Principle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

<div>The approach to inhibit endogenous peroxidases by elevated concentrations of hydrogen peroxide while maintaining the high peroxidase-mimicking activity of Au@Pt nanozymes was developed. The approach facilitates selective and highly-sensitive detection of peroxidase-mimicking nanozyme nanozymes in the background of endogenous peroxidases. Au@Pt nanozyme was used as the colorimetric and catalytic label in lateral flow immunoassay of an important plant pathogen – potato virus X. The inhibition of endogenous peroxidases in plant extracts and selective detection of Au@Pt nanozyme provides the lowest limit of detection among immunochemical assays of potato virus X (up to 500 times lower compared to the assay with conventional gold nanoparticles). </div><div>The proposed approach uses the fundamental principle of enzyme inhibition by the substrate. It is universal and applicable to all matrixes with peroxidase activity. </div>

Determination of Lead (II) Concentration Using Iminodiacetic Acid-Modified Silver Nanoparticles by Highly Sensitive SERS Technique

NANO ◽  
2017 ◽  
Vol 12 (07) ◽  
pp. 1750090
Author(s):  
Na Li ◽  
Yaoyong Meng ◽  
Yongfang Dong ◽  
Zhiyou Guo ◽  
Nan Li
Keyword(s):  
Silver Nanoparticles ◽  
Limit Of Detection ◽  
Iminodiacetic Acid ◽  
High Sensitivity ◽  
World Health ◽  
Raman Signal ◽  
Highly Sensitive ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Highly Sensitive Detection

A system for the highly sensitive detection of Pb[Formula: see text] ions by surface-enhanced Raman scattering (SERS) was designed based on silver nanoparticles (AgNPs) capped with iminodiacetic acid (IDA)/4-mercaptopyridine (4-MPY). In this system, IDA was absorbed on the surface of the AgNPs to induce the coupling of Pb[Formula: see text] ions for aggregation in the aqueous solution. 4-MPY, which was also attached to the surface of the AgNPs, was used as a Raman reporter. When the concentration of Pb[Formula: see text] ions increased, a stronger 4-MPY Raman signal was detected. The IDA and 4-MPY concentrations were adjusted to maximize the 4-MPY Raman signal. Under this optimal condition, the concentration of Pb[Formula: see text] ions had a linear relationship with 4-MPY Raman signal in the range of 1[Formula: see text][Formula: see text][Formula: see text]108 to [Formula: see text][Formula: see text]mol/L ([Formula: see text]). The limit of detection (LOD) of 1.816[Formula: see text]nmol/L was obtained, which is significantly lower than the World Health Organization’s (WHO) defined limit of 100[Formula: see text][Formula: see text]g/L in drinking water. This system was successfully applied to determine the Pb[Formula: see text] concentration in a contaminated water sample, which is obtained by adding a trace of Pb[Formula: see text] ions to drinking mineral water. The results proved that the IDA/4-MPY-modified AgNPs sensing system had a potential application for the Pb[Formula: see text] measurement with high sensitivity and excellent selectivity.

scholarly journals Highly sensitive detection of nitrobenzene by a series of fluorescent 2D zinc(ii) metal–organic frameworks with a flexible triangular ligand

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23975-23984
Author(s):  
Xue Yang ◽  
Yixia Ren ◽  
Hongmei Chai ◽  
Xiufang Hou ◽  
Zhixiang Wang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Metal Organic Frameworks ◽  
High Sensitivity ◽  
Fluorescent Sensors ◽  
Sensitive Detection ◽  
Donor Ligands ◽  
Highly Sensitive ◽  
Metal Organic ◽  
Highly Sensitive Detection

Four fluorescent 2D Zn-MOFs based on a flexible triangular ligand and linear N-donor ligands are hydrothermally prepared and used to detect nitrobenzene in aqueous solution with high sensitivity, demonstrating their potential as fluorescent sensors.

scholarly journals Highly sensitive and selective colorimetric detection of dual metal ions (Hg2+ and Sn2+) in water: an eco-friendly approach

RSC Advances ◽  
2021 ◽  
Vol 11 (24) ◽  
pp. 14700-14709
Author(s):  
Rintumoni Paw ◽  
Moushumi Hazarika ◽  
Purna K. Boruah ◽  
Amlan Jyoti Kalita ◽  
Ankur K. Guha ◽  
...  
Keyword(s):  
Metal Ions ◽  
Ag Nanoparticles ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
Garlic Extract ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Dual Metal

Synthesis of Ag nanoparticles using Allin based garlic extract for highly sensitive and selective detection of metal ions Hg2+ and Sn2+ in water. The limit of detection (LoD) for Hg2+ and Sn2+ ions were found as 15.7 nM and 11.25 nM respectively.

Highly sensitive detection of Cr(vi) in groundwater by bimetallic NiFe nanoparticles

2017 ◽  
Vol 9 (6) ◽  
pp. 1031-1037 ◽  
Author(s):  
Jingtao Liu ◽  
Yu Ding ◽  
Lifei Ji ◽  
Xin Zhang ◽  
Fengchun Yang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Hexavalent Chromium ◽  
Low Cost ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Toxic Heavy Metal ◽  
Metal Pollutants ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Heavy Metal Pollutants

Hexavalent chromium (Cr(vi)) is one of the most toxic heavy metal pollutants in groundwater, and thus the detection of Cr(vi) with high sensitivity, accuracy, and simplicity and low cost is of great importance.

scholarly journals A Highly Sensitive Cell-Based TLR Reporter Platform for the Specific Detection of Bacterial TLR Ligands

2022 ◽  
Vol 12 ◽  
Author(s):  
Katharina Radakovics ◽  
Claire Battin ◽  
Judith Leitner ◽  
Sabine Geiselhart ◽  
Wolfgang Paster ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Ectopic Expression ◽  
High Sensitivity ◽  
High Specificity ◽  
Reporter System ◽  
Specificity And Sensitivity ◽  
Highly Sensitive ◽  
Definition Of ◽  
The Individual ◽  
Allergen Extracts

Toll-like receptors (TLRs) are primary pattern recognition receptors (PRRs), which recognize conserved microbial components. They play important roles in innate immunity but also in the initiation of adaptive immune responses. Impurities containing TLR ligands are a frequent problem in research but also for the production of therapeutics since TLR ligands can exert strong immunomodulatory properties even in minute amounts. Consequently, there is a need for sensitive tools to detect TLR ligands with high sensitivity and specificity. Here we describe the development of a platform based on a highly sensitive NF-κB::eGFP reporter Jurkat JE6-1 T cell line for the detection of TLR ligands. Ectopic expression of TLRs and their coreceptors and CRISPR/Cas9-mediated deletion of endogenously expressed TLRs was deployed to generate reporter cell lines selectively expressing functional human TLR2/1, TLR2/6, TLR4 or TLR5 complexes. Using well-defined agonists for the respective TLR complexes we could demonstrate high specificity and sensitivity of the individual reporter lines. The limit of detection for LPS was below 1 pg/mL and ligands for TLR2/1 (Pam3CSK4), TLR2/6 (Fsl-1) and TLR5 (flagellin) were detected at concentrations as low as 1.0 ng/mL, 0.2 ng/mL and 10 pg/mL, respectively. We showed that the JE6-1 TLR reporter cells have the utility to characterize different commercially available TLR ligands as well as more complex samples like bacterially expressed proteins or allergen extracts. Impurities in preparations of microbial compounds as well as the lack of specificity of detection systems can lead to erroneous results and currently there is no consensus regarding the involvement of TLRs in the recognition of several molecules with proposed immunostimulatory functions. This reporter system represents a highly suitable tool for the definition of structural requirements for agonists of distinct TLR complexes.

An electrochemical sensor for highly sensitive detection of copper ions based on a new molecular probe Pi-A decorated on graphene

2017 ◽  
Vol 9 (4) ◽  
pp. 618-624 ◽  
Author(s):  
Liuqing Yang ◽  
Na Huang ◽  
Liyan Huang ◽  
Meiling Liu ◽  
Haitao Li ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Electrochemical Method ◽  
Copper Ions ◽  
Molecular Probe ◽  
Selective Detection ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

In this paper, a new electrochemical sensor based on a new fluorescent probe N-(2-(1-(p-tolyl)-1H-phenanthro[9,10-d]imidazol-2-yl)phenyl)picolinamide (Pi-A) decorated reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) was developed for highly selective detection of copper ions using the electrochemical method.

scholarly journals Development of micropillar array electrodes for highly sensitive detection of biomarkers

RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 41110-41119
Author(s):  
Chaozhan Chen ◽  
Bin Ran ◽  
Zhenxing Wang ◽  
Hongli Zhao ◽  
Minbo Lan ◽  
...  
Keyword(s):  
Surface Modification ◽  
Surface Area ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Detection Of Biomarkers ◽  
Highly Sensitive Detection ◽  
Micropillar Array

PDMS-based micropillar array electrodes with increased surface area and surface modification were developed to detect biomarkers with high sensitivity.

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