Tuning the Thermogelation and Rheology of Poly(2-Oxazoline)/poly(2-Oxazine)s Based Thermosensitive Hydrogels for 3D Bioprinting.

As one kind of smart material, thermogelling polymers find applications in biofabrication, drug delivery and regenerative medicine. Here, we reported on a novel thermosensitive hydrogel which can be 3D printed using extrusion based printing. Gel strength was found around 3kPa storage modulus with pronounced shear thinning and rapid recovery after stress. Addition of clay nanoparticles (Laponite XLG) improved the rheological profile further. Human adipose derived stem cells were added to the hydrogel matrix, which remained fully viable after printing. Therefore, the presented materials adds to the available material toolbox for 3D bioprinting. <br>

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3D-Bioprinting of Polylactic Acid (PLA) Nanofiber–Alginate Hydrogel Bioink Containing Human Adipose-Derived Stem Cells

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.6b00196 ◽

2016 ◽

Vol 2 (10) ◽

pp. 1732-1742 ◽

Cited By ~ 101

Author(s):

Lokesh Karthik Narayanan ◽

Pedro Huebner ◽

Matthew B. Fisher ◽

Jeffrey T. Spang ◽

Binil Starly ◽

...

Keyword(s):

Stem Cells ◽

Polylactic Acid ◽

Adipose Derived Stem Cells ◽

3D Bioprinting ◽

Alginate Hydrogel

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3D Bioprinting of Human Adipose-Derived Stem Cells and Their Tenogenic Differentiation in Clinical-Grade Medium

International Journal of Molecular Sciences ◽

10.3390/ijms21228694 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8694

Author(s):

Deborah Stanco ◽

Monica Boffito ◽

Alessia Bogni ◽

Luca Puricelli ◽

Josefa Barrero ◽

...

Keyword(s):

Stem Cells ◽

Cell Viability ◽

Scale Up ◽

Good Manufacturing Practice ◽

Adipose Derived Stem Cells ◽

3D Bioprinting ◽

Clinical Scale ◽

Nanofibrillar Cellulose ◽

Tenogenic Differentiation ◽

Tendon Tissue Engineering

Defining the best combination of cells and biomaterials is a key challenge for the development of tendon tissue engineering (TE) strategies. Adipose-derived stem cells (ASCs) are ideal candidates for this purpose. In addition, controlled cell-based products adherent to good manufacturing practice (GMP) are required for their clinical scale-up. With this aim, in this study, ASC 3D bioprinting and GMP-compliant tenogenic differentiation were investigated. In detail, primary human ASCs were embedded within a nanofibrillar-cellulose/alginate bioink and 3D-bioprinted into multi-layered square-grid matrices. Bioink viscoelastic properties and scaffold ultrastructural morphology were analyzed by rheology and scanning electron microscopy (SEM). The optimal cell concentration for printing among 3, 6 and 9 × 106 ASC/mL was evaluated in terms of cell viability. ASC morphology was characterized by SEM and F-actin immunostaining. Tenogenic differentiation ability was then evaluated in terms of cell viability, morphology and expression of scleraxis and collagen type III by biochemical induction using BMP-12, TGF-β3, CTGF and ascorbic acid supplementation (TENO). Pro-inflammatory cytokine release was also assessed. Bioprinted ASCs showed high viability and survival and exhibited a tenocyte-like phenotype after biochemical induction, with no inflammatory response to the bioink. In conclusion, we report a first proof of concept for the clinical scale-up of ASC 3D bioprinting for tendon TE.

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Modified PLGA–PEG–PLGA thermosensitive hydrogels with suitable thermosensitivity and properties for use in a drug delivery system

Journal of Materials Chemistry B ◽

10.1039/c6tb02158a ◽

2017 ◽

Vol 5 (8) ◽

pp. 1551-1565 ◽

Cited By ~ 34

Author(s):

Puxiu Wang ◽

Wei Chu ◽

Xuezhi Zhuo ◽

Yu Zhang ◽

Jingxin Gou ◽

...

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Triblock Copolymer ◽

Thermosensitive Hydrogel ◽

System P ◽

Thermosensitive Hydrogels

PLGA–PEG–PLGA (PPP) triblock copolymer is the most widely studied thermosensitive hydrogel owing to its non-toxic, biocompatible, biodegradable, and thermosensitive properties.

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Osteogenesis of 3D-Printed PCL/TCP/bdECM Scaffold Using Adipose-Derived Stem Cells Aggregates; An Experimental Study in the Canine Mandible

International Journal of Molecular Sciences ◽

10.3390/ijms22115409 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5409

Author(s):

Joon Seok Lee ◽

Tae Hyun Park ◽

Jeong Yeop Ryu ◽

Dong Kyu Kim ◽

Eun Jung Oh ◽

...

Keyword(s):

Stem Cells ◽

Intervention Group ◽

Three Dimensional ◽

Artificial Organs ◽

Adipose Derived Stem Cells ◽

Gene Expressions ◽

Group A ◽

3D Printed ◽

Group B ◽

Tissue Recovery

Three-dimensional (3D) printing is perceived as an innovative tool for change in tissue engineering and regenerative medicine based on research outcomes on the development of artificial organs and tissues. With advances in such technology, research is underway into 3D-printed artificial scaffolds for tissue recovery and regeneration. In this study, we fabricated artificial scaffolds by coating bone demineralized and decellularized extracellular matrix (bdECM) onto existing 3D-printed polycaprolactone/tricalcium phosphate (PCL/TCP) to enhance osteoconductivity and osteoinductivity. After injecting adipose-derived stem cells (ADSCs) in an aggregate form found to be effective in previous studies, we examined the effects of the scaffold on ossification during mandibular reconstruction in beagle dogs. Ten beagles were divided into two groups: group A (PCL/TCP/bdECM + ADSC injection; n = 5) and group B (PCL/TCP/bdECM; n = 5). The results were analyzed four and eight weeks after intervention. Computed tomography (CT) findings showed that group A had more diffuse osteoblast tissue than group B. Evidence of infection or immune rejection was not detected following histological examination. Goldner trichrome (G/T) staining revealed rich ossification in scaffold pores. ColI, Osteocalcin, and Runx2 gene expressions were determined using real-time polymerase chain reaction. Group A showed greater expression of these genes. Through Western blotting, group A showed a greater expression of genes that encode ColI, Osteocalcin, and Runx2 proteins. In conclusion, intervention group A, in which the beagles received the additional ADSC injection together with the 3D-printed PCL/TCP coated with bdECM, showed improved mandibular ossification in and around the pores of the scaffold.

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Using 3D‐bioprinting scaffold loaded with adipose‐derived stem cells (ADSC) to burns wound healing.

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.3194 ◽

2021 ◽

Author(s):

Leila Roshangar ◽

Jafar Soleimani Rad ◽

Razie Kheirjou ◽

Ahad Ferdowsi Khosroshahi

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Adipose Derived Stem Cells ◽

3D Bioprinting

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Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Applied Sciences ◽

10.3390/app10072455 ◽

2020 ◽

Vol 10 (7) ◽

pp. 2455 ◽

Cited By ~ 4

Author(s):

Jongbeom Ku ◽

Hoon Seonwoo ◽

Sangbae Park ◽

Kyoung-Je Jang ◽

Juo Lee ◽

...

Keyword(s):

Cell Viability ◽

Gelation Time ◽

Three Dimensional ◽

Gelling Agent ◽

Patient Specific ◽

3D Bioprinting ◽

Thermosensitive Hydrogel ◽

Chitosan Hydrogel ◽

Covalent Interaction ◽

Thermosensitive Hydrogels

Three-dimensional (3D) bioprinting is a technology used to deposit cell-laden biomaterials for the construction of complex tissues. Thermosensitive hydrogels are physically cross-linked by non-covalent interaction without using crosslinkers, facilitating low cytotoxicity and cell viability. Chitosan, which is a non-toxic, biocompatible and biodegradable polysaccharide, can be used as a thermosensitive hydrogel. Therefore, chitosan hydrogel could be of potential use as a 3D bioprinting ink. The purpose of this study was to develop and compare the effectivity of different bioinks based on chitosan hydrogels for 3D bioprinting. The solvent type did not affect the gel shape and gelation time, whereas acetic acid exhibited better biocompatibility compared to lactic and hydrochloric acids. The nature of the gelling agent was found to have a stronger influence on these characteristics than that of the solvent. The NaHCO3 moiety exhibited a higher growth rate of the storage modulus (G′) and a more irregular porous structure than that of the β-glycerophosphate (β-GP) and K2HPO4 groups. Cell viability, and live and dead assays, showed that the NaHCO3 group was more efficient for cell adhesion. The type of gelling agent did not lead to appreciable differences in cell-laden constructs. The NaHCO3 group was more amenable to bioprinting, compared to the β-GP and K2HPO4 groups. The chitosan hydrogel bioinks could, therefore, be good candidates for 3D bioprinting and would pave the way for patient-specific regenerative medicines.

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