Ultrasound-assisted extraction of medicinal plants and evaluation of their biological activity

Deep eutectic solvents (DESs) are considered as efficient and green solvents for the extraction of bioactive compounds from medicinal plants. In this work, a novel method of DES-based ultrasound-assisted extraction of bioactive compounds from Baphicacanthus cusia leaves (BCL) was established. Systematic screening and the morphology of the original and treated BCL were observed with scanning electron microscopy to determine the extraction efficiency of different solvents. The extraction conditions were optimized by Box–Behnken design (BBD) tests and the optimal extraction conditions were as follows: lactic acid/L-menthol ratio of 5: 2 (mol/mol), solid–liquid ratio of 80.0 mL/g and temperature of 60.5 °C. The extraction yields of tryptanthrin, indigo and indirubin reached 0.356, 1.744 and 0.562 mg/g, respectively. The results of a 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity test indicated the feasibility of DESs in the extraction of bioactive compounds. This study indicated that L-menthol/lactic acid was a green and efficient solvent for the extraction of bioactive compounds from BCL, and DES-based ultrasound-assisted extraction could be used as an effective application strategy for the extraction of bioactive compounds from medicinal plants.

Download Full-text

Ultrasound-assisted extraction of polysaccharides from brown alga Fucus evanescens. Structure and biological activity of the new fucoidan fractions.

Journal of Applied Phycology ◽

10.1007/s10811-017-1342-9 ◽

2017 ◽

Vol 30 (3) ◽

pp. 2039-2046 ◽

Cited By ~ 13

Author(s):

Andrey B. Hmelkov ◽

Tatiana N. Zvyagintseva ◽

Natalia M. Shevchenko ◽

Anton B. Rasin ◽

Svetlana P. Ermakova

Keyword(s):

Biological Activity ◽

Brown Alga ◽

Ultrasound Assisted Extraction ◽

Fucus Evanescens ◽

Assisted Extraction ◽

Ultrasound Assisted

Download Full-text

Ultrasound-Assisted Extraction and Assessment of Biological Activity of Phycobiliprotein-Rich Aqueous Extracts from Wild Cyanobacteria (Aphanizomenon flos-aquae)

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.9b05483 ◽

2019 ◽

Vol 68 (7) ◽

pp. 1896-1909

Author(s):

Michail Syrpas ◽

Jolita Bukauskaitė ◽

Kristina Ramanauskienė ◽

Ju̅ratė Karosienė ◽

Daiva Majienė ◽

...

Keyword(s):

Biological Activity ◽

Aqueous Extracts ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Download Full-text

Combination of Lactic Acid-Based Deep Eutectic Solvents (DES) with β-Cyclodextrin: Performance Screening Using Ultrasound-Assisted Extraction of Polyphenols from Selected Native Greek Medicinal Plants

Agronomy ◽

10.3390/agronomy7030054 ◽

2017 ◽

Vol 7 (3) ◽

pp. 54 ◽

Cited By ~ 22

Author(s):

Chrysa Georgantzi ◽

Antonia-Eleni Lioliou ◽

Nikos Paterakis ◽

Dimitris Makris

Keyword(s):

Lactic Acid ◽

Medicinal Plants ◽

Deep Eutectic Solvents ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Download Full-text

Novel lactic acid-based natural deep eutectic solvents: Efficiency in the ultrasound-assisted extraction of antioxidant polyphenols from common native Greek medicinal plants

Journal of Applied Research on Medicinal and Aromatic Plants ◽

10.1016/j.jarmap.2016.03.003 ◽

2016 ◽

Vol 3 (3) ◽

pp. 120-127 ◽

Cited By ~ 49

Author(s):

Christina Bakirtzi ◽

Katerina Triantafyllidou ◽

Dimitris P. Makris

Keyword(s):

Lactic Acid ◽

Medicinal Plants ◽

Deep Eutectic Solvents ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Natural Deep Eutectic Solvents ◽

Ultrasound Assisted

Download Full-text

Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

Advances in Nutrition & Food Science ◽

10.33140/anfs.04.02.2 ◽

2019 ◽

Vol 4 (2) ◽

Keyword(s):

Phenolic Compounds ◽

Carcinoma Cell ◽

Biological Activities ◽

Peanut Meal ◽

Ultrasound Assisted Extraction ◽

Carcinoma Cell Lines ◽

Assisted Extraction ◽

Phenolic Extract ◽

Ultrasound Assisted ◽

Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

Download Full-text