scholarly journals Optimization of Extraction of Bioactive Compounds from Baphicacanthus cusia Leaves by Hydrophobic Deep Eutectic Solvents

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1729
Author(s):  
Zhiqin Xu ◽  
Yini Cai ◽  
Qing Ma ◽  
Zhimin Zhao ◽  
Depo Yang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Medicinal Plants ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Deep Eutectic Solvents ◽  
Green Solvents ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Deep eutectic solvents (DESs) are considered as efficient and green solvents for the extraction of bioactive compounds from medicinal plants. In this work, a novel method of DES-based ultrasound-assisted extraction of bioactive compounds from Baphicacanthus cusia leaves (BCL) was established. Systematic screening and the morphology of the original and treated BCL were observed with scanning electron microscopy to determine the extraction efficiency of different solvents. The extraction conditions were optimized by Box–Behnken design (BBD) tests and the optimal extraction conditions were as follows: lactic acid/L-menthol ratio of 5: 2 (mol/mol), solid–liquid ratio of 80.0 mL/g and temperature of 60.5 °C. The extraction yields of tryptanthrin, indigo and indirubin reached 0.356, 1.744 and 0.562 mg/g, respectively. The results of a 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity test indicated the feasibility of DESs in the extraction of bioactive compounds. This study indicated that L-menthol/lactic acid was a green and efficient solvent for the extraction of bioactive compounds from BCL, and DES-based ultrasound-assisted extraction could be used as an effective application strategy for the extraction of bioactive compounds from medicinal plants.

scholarly journals Natural antioxidants isolated from Schinus areira leaves by ultrasound-assisted extraction

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Liliana S. Celaya ◽  
Carmen I. Viturro ◽  
Luís R. Silva ◽  
Silvia Moreno
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Leaf Extracts ◽  
Chromatography Analysis ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Optimized Conditions

The aim of this study was to optimize the extraction of antioxidant compounds from Schinus areira leaves using  ultrasound assisted extraction and response surface methodology. The effect of sonication time and plant material:solvent ratio were used to optimize the recovery. Results showed that a high recovery of antioxidant compounds from leaves of three different S. areira specimens was achieved under optimized conditions. The leaf extracts obtained displayed a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity analogous to the well-known antioxidant trolox  (EC50 = 23-46 vs 36.1 µg/mL, respectively). In addition, these extracts showed a good potency to eliminate superoxide and nitric oxide-radicals as well as a moderate antimicrobial activity against gram positive Staphylococcus aureus and Enterococcus faecalis and yeast. HPLC chromatography analysis of the three S. areira leaf extracts showed different high contents of kaempferol-3-O-rutinoside, quercetin-3-O-galactoside and 3-O-caffeoylquinic acid. The results showed that the S. areira leaf extracts contained a high amount of antioxidant phenolic compounds, which might be a valuable source to be used as additives in plant-based foods.

scholarly journals Green solvents and Ultrasound-Assisted Extraction of bioactive orange (Citrus sinensis) peel compounds

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Abigail Montero-Calderon ◽  
Clara Cortes ◽  
Ana Zulueta ◽  
Ana Frigola ◽  
Maria J. Esteve
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Orange Peel ◽  
Green Solvents ◽  
Ultrasound Assisted Extraction ◽  
Total Carotenoids ◽  
Carotenoid Concentration ◽  
Assisted Extraction ◽  
Physicochemical Evaluation ◽  
Ultrasound Assisted

Abstract Byproducts such as orange peel have potential uses because of their bioactive compounds, which are important for their potential to reduce the risk factors of diseases caused by aging. The lack of effective techniques and the high levels of pollution produced by the conventional extraction of bioactive compounds using organic solvents have highlighted the need to enhance the ‘green chemistry’ trend. This study evaluates the use of ultrasound to extract bioactive compounds from orange peel. The antioxidant capacity, phenolic content, ascorbic acid, total carotenoids, and HPLC profile of phenolic compounds from orange peel extracts were obtained by a physicochemical evaluation. The results demonstrate that the optimal conditions for the ultrasound-assisted extraction of bioactive orange peel compounds were a power of 400 W, a time of 30 min, and 50% ethanol in water. These conditions were used to obtain a total carotenoid concentration of 0.63 mg ß-carotene/100 g, vitamin C concentration of 53.78 mg AA/100 g, phenolic concentration of 105.96 mg GAE/100 g, and antioxidant capacity of ORAC = 27.08 mM TE and TEAC = 3.97 mM TE. The major phenolic compound identified in all orange peel extracts was hesperidin, with a maximum concentration of 113.03 ± 0.08 mg/100 g.

scholarly journals Natural deep eutectic solvents as new green solvents to extract anthraquinones from Rheum palmatum L.

RSC Advances ◽  
2018 ◽  
Vol 8 (27) ◽  
pp. 15069-15077 ◽  
Author(s):  
Y. C. Wu ◽  
P. Wu ◽  
Y. B. Li ◽  
T. C. Liu ◽  
L. Zhang ◽  
...  
Keyword(s):  
Deep Eutectic Solvents ◽  
Green Solvents ◽  
Ultrasound Assisted Extraction ◽  
Rheum Palmatum ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasound Assisted

Natural deep eutectic solvents ultrasound-assisted extraction (NADES-UAE) was applied to extract total anthraquinones from Rheum palmatum L.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

2019 ◽  
Vol 92 (3) ◽  
pp. 369-377
Author(s):  
Barbara Fumić ◽  
Mario Jug ◽  
Marijana Zovko Končić
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lotus Corniculatus ◽  
Extraction Yield ◽  
Phenolic Antioxidants ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box–Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) IC50 and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg mL–1 and 6.69 to 92.75 mg mL–1, respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L. corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant.

scholarly journals Ultrasound-assisted extraction of bioactive compounds from lemon balm and peppermint leaves

2016 ◽  
Vol 30 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Jana Šic Žlabur ◽  
Sandra Voća ◽  
Nadica Dobričević ◽  
Stjepan Pliestić ◽  
Ante Galić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Ultrasonic Extraction ◽  
Total Phenolic ◽  
Total Phenols ◽  
Ultrasound Assisted Extraction ◽  
Lemon Balm ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Abstract The aim of this study was to investigate the influence of conventional and ultrasound-assisted extraction (frequency, time, temperature) on the content of bioactive compounds as well as on the antioxidant activity of aqueous extracts from fresh lemon balm and peppermint leaves. Total phenols, flavonoids, non-flavonoids, total chlorophylls, total carotenoids, and radical scavenging capacity were determined. Moreover, the relationship between bioactive compounds and antioxidant capacity was studied by linear regression. A significant increase in all studied bioactive compounds during ultrasonic extraction for 5 to 20 min was found. With the classical extraction method, the highest amounts of total phenols, flavonoids, and antioxidant activity were determined, and the maximum amounts of total chlorophylls and carotenoids were determined during 20 min ultrasonic extraction. The correlation analysis revealed a strong, positive relationship between antioxidant activity and total phenolic compounds.

scholarly journals Green Ultrasound Assisted Extraction of trans Rosmarinic Acid from Plectranthus scutellarioides (L.) R.Br. Leaves

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 50 ◽  
Author(s):  
Duangjai Tungmunnithum ◽  
Laurine Garros ◽  
Samantha Drouet ◽  
Sullivan Renouard ◽  
Eric Lainé ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ultrasound Assisted Extraction ◽  
Pure Ethanol ◽  
Ultrasound Frequency ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Painted nettle (Plectranthus scutellarioides (L.) R.Br.) is an ornamental plant belonging to Lamiaceae family, native of Asia. Its leaves constitute one of the richest sources of trans-rosmarinic acid, a well-known antioxidant and antimicrobial phenolic compound. These biological activities attract interest from the cosmetic industry and the demand for the development of green sustainable extraction processes. Here, we report on the optimization and validation of an ultrasound-assisted extraction (USAE) method using ethanol as solvent. Following preliminary single factor experiments, the identified limiting extraction parameters (i.e., ultrasound frequency, extraction duration, and ethanol concentration) were further optimized using a full factorial design approach. The method was then validated following the recommendations of the association of analytical communities (AOAC) to ensure the precision and accuracy of the method used to quantify trans-rosmarinic acid. Highest trans-rosmarinic acid content was obtained using pure ethanol as extraction solvent following a 45-minute extraction in an ultrasound bath operating at an ultrasound frequency of 30 kHz. The antioxidant (in vitro radical scavenging activity) and antimicrobial (directed toward Staphylococcus aureus ACTT6538) activities were significantly correlated with the trans-rosmarinic acid concentration of the extract evidencing that these key biological activities were retained following the extraction using this validated method. Under these conditions, 110.8 mg/g DW of trans-rosmarinic acid were extracted from lyophilized P. scutellarioides leaves as starting material evidencing the great potential of this renewable material for cosmetic applications. Comparison to other classical extraction methods evidenced a clear benefit of the present USAE method both in terms of yield and extraction duration.

scholarly journals Optimization of ultrasound-assisted extraction of bioactive compounds from Gomphrena celosioides Mart. using response surface methodology

2020 ◽  
Vol 34 (2) ◽  
pp. 237-248
Author(s):  
N. T. H. Yen ◽  
L. P. T. Quoc
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Gomphrena Celosioides ◽  
Experimental Values

In this study, ultrasound-assisted extraction (UAE) was used to extract bioactive compounds from Gomphrena celosioides Mart. Central composite face design (CCF) was used to optimize the influences of extraction factors on total phenolics content (TPC), total flavonoids content (TFC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging capacity (DPPH-RSC). The results obtained showed that all factors (solvent to solid ratio, extraction time and extraction temperature) strongly affected TPC, TFC, and DPPH-RSC. The optimal extraction conditions for TPC (3.123 mg GAE/g DW), TFC (1.736 mg QE/g DW), and DPPH-RSC (64.118%) were solvent to solid ratio of 27.3/1 (mL/g), extraction time of 40.2 min, and extraction temperature of 69 oC. In addition, under the optimal conditions, the results pointed out that the experimental values agreed with those predicted. Hence, this model has successfully optimized the extraction conditions for TPC, TFC and DPPH-RSC of Gomphrena celosioides extract.                     KEY WORDS: Antioxidant activity, CCF, Flavonoids, Model, Phenolic compounds, Regression equation   Bull. Chem. Soc. Ethiop. 2020, 34(2), 237-248 DOI: https://dx.doi.org/10.4314/bcse.v34i2.3

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