scholarly journals Eosinophil cationic protein as a serological marker of disease activity in childhood bronchial asthma

1999 ◽  
Vol 5 (4) ◽  
pp. 664-675
Author(s):  
O. M. Badr El Din ◽  
I. H. El Sawy ◽  
O. E. El Azzouni ◽  
M. M. A. Badr El Din ◽  
A. M. Salem
Keyword(s):  
Disease Activity ◽  
Bronchial Asthma ◽  
Eosinophil Cationic Protein ◽  
Serological Marker ◽  
Control Group ◽  
Cationic Protein ◽  
Asthmatic Children ◽  
Asthmatic Patients ◽  
Healthy Control ◽  
The Mean

To study the value of eosinophil cationic protein [ECP] as a serological marker of disease activity in childhood bronchial asthma, ECP levels were measured in 20 healthy control children and 25 asthmatic children, during and 2 weeks after acute exacerbation. The mean serum ECP level of all asthmatic patients, during and after exacerbation, was significantly higher than the control group and was significantly higher during attacks than 2 weeks after their termination. ECP levels were highest in severe attacks, but did not differ between mild and moderate attacks. ECP levels in asthmatic patients 2 weeks after mild and moderate attacks were comparable to normal; after severe attacks levels remained higher than normal. Measurement of serum ECP will be helpful in determining asthma activity and deciding the use of anti-asthma drugs

scholarly journals Eosinophil cationic protein (ECP) in saliva: a new marker of disease activity in bronchial asthma

2001 ◽  
Vol 95 (8) ◽  
pp. 670-675 ◽  
Author(s):  
B. SCHMEKEL ◽  
J. AHLNER ◽  
M. MALMSTRÖM ◽  
P. VENGE
Keyword(s):  
Disease Activity ◽  
Bronchial Asthma ◽  
Eosinophil Cationic Protein ◽  
Cationic Protein

scholarly journals The serum eosinophil cationic protein concentration in patients with bronchial asthma and its correlation with severity and exacerbation of the disease

2003 ◽  
Vol 22 (3) ◽  
pp. 249-253
Author(s):  
Snezana Kovacevic ◽  
Mirjana Bogic ◽  
Aleksandra Peric-Popadic ◽  
Sanvila Raskovic ◽  
Zikica Jovicic ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Clinical Picture ◽  
Healthy Subjects ◽  
Protein Concentration ◽  
Geometric Mean ◽  
Eosinophil Cationic Protein ◽  
Cationic Protein ◽  
Asthmatic Patients ◽  
Severity Of Disease ◽  
Severity Of The Disease

Activated eosinophilic leukocyte in asthma secretes numerous mediators, among which is ECP as well. The object of our study was to measure the serum ECP concentrations in 46 asthmatic patients with exacerabating and stable asthma, and to correlate the serum ECP concentrations with severity and exacerbation of the disease. Geometric mean of ECP in serum (Gecp) in our group of patients was 7.5 mcg/l, while it was 3.05 mcg/l in the 15 healthy subjects (controls). Highly significant correlation of serum ECP concentrations with the activity of the disease (R=0.897) and the severity of clinical picture (R=0.79) was found. The patients with stable asthma had significant correlation of ECP and the severity of disease (R=0.6). The patients with exacerbating asthma have significantly higher serum ECP concentrations than the patients with stable asthma. Serum ECP concentrations in patients with exacerbating asthma correlate with the severity of the disease.

The relationship of serum-eosinophil cationic protein and eosinophil count to disease activity in children with bronchial asthma

1998 ◽  
Vol 9 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Antje Prehn ◽  
Reinhard A. Seger ◽  
Joerg Faber ◽  
Toni Torresani ◽  
Luciano Molinari ◽  
...  
Keyword(s):  
Disease Activity ◽  
Bronchial Asthma ◽  
Eosinophil Count ◽  
Eosinophil Cationic Protein ◽  
Cationic Protein ◽  
Relationship Of ◽  
The Relationship

scholarly journals The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma

Inflammation ◽  
2021 ◽  
Author(s):  
Suhayla H. Shareef ◽  
Kawa Amin ◽  
Christer Janson
Keyword(s):  
Smooth Muscle ◽  
Allergic Asthma ◽  
Airway Smooth Muscle ◽  
Airway Remodeling ◽  
Eosinophil Cationic Protein ◽  
Healthy Control Group ◽  
Control Group ◽  
Bronchial Biopsy ◽  
Cationic Protein ◽  
Healthy Control

Abstract Allergic asthma (AA) is a complex disorder with heterogeneous features of airway hyperresponsiveness, inflammation, and remodeling. The increase of airway smooth muscle (ASM) mass is a fundamental component of bronchial remodeling in AA, yet the pathophysiological mechanisms and clinical outcomes associated with ASM modulation are still elusive. The objective of this study is to compare the expression level of β-dystroglycan (β-DG) in ASM in AA subjects and a healthy control group and to investigate the relationship between eosinophils and β-DG in ASM in patients with AA. Thirteen AA patients and seven control subjects were analyzed for the ASM area and eosinophil cells. Bronchial biopsies were stained by β-DG and eosinophil cationic protein (ECP) using immunohistochemistry. The proportion of ASM with β-DG staining was greater in those with AA than in the healthy control group (mean (95% CI) (28.3% (23.8–32.7%) vs. 16.4% (14.1–18.5%), P < 0.0001). The number of ECP positive cells was higher in patients with AA than in the control group (4056 (3819–4296) vs. 466 (395–537) cells/mm2P < 0.0001). In AA, the number of ECP positive cells was significantly correlated to the β-DG expression in ASM (r = 0.77, P = 0.002). There is an increased β-DG expression in ASM and a higher number of ECP positive cells in the bronchial biopsy of those with AA than those in the control group. The increased expression of β-DG in ASM in AA subjects correlates with the number of eosinophils, suggesting a role for this cell in airway remodeling in AA.

scholarly journals EOSINOPHIL CATIONIC PROTEIN AND IRON STATUS IN PATIENTS INFECTED WITH Enterobius vermicularis

2021 ◽  
Vol 9 (5) ◽  
pp. 672-677
Author(s):  
Esraa Wathah ◽  
◽  
Saleem Khteer Al-Hadraawy ◽  
Keyword(s):  
Serological Test ◽  
Iron Status ◽  
Eosinophil Cationic Protein ◽  
Control Group ◽  
Enterobius Vermicularis ◽  
Serum Samples ◽  
Cationic Protein ◽  
Ferritin Concentration ◽  
Healthy Control ◽  
Elisa Technique

The primary goal of this study was to determine the function of eosinophil cationic protein in iron status in patients infected with Enterobius vermicularis. For this, a total of 583 suspected patients and thirty healthy people of the same age who have visited the AL-Zahra maternity and pediatrics laboratory, AL-Hakeem hospital, AL-Sajad hospital AL-Fruit al-Awsat hospital, and AL-Munadira hospital in AL-Najaf province from July 2020 to June 2021 were screened. The presence of E. vermicularis eggs was estimated by using the saline wet mount technique from faeces samples of all respondents. Blood samples were collected from the 60 positive and 30 healthy control group and centrifuged at 3000 rpm for 5 minutes to separate serum, which was then collected in sterile tubes. Each serum sample was divided into three parts and stored in the deep freezer at -20°C until the serological test was performed. The level of ECP, iron and ferritin in enterobiasis patients was estimated from the isolated blood serum. According to the Manufacturer Company instructions, the concentration of two biomarkers (ECP, Ferritin) in serum samples was determined using the ELISA technique (Human reader, Germany). While the concentration of iron was evaluated using a colourimetric method. In comparison to the control group, the concentration of ECP was reported significantly higher (P<0.05) in the E. vermiculris infected patients while the concentration of serum iron and ferritin was significantly decreased. The results of the current study can be concluded that E. vermicularis infection changes the serum ECP, iron, and ferritin concentration.

scholarly journals Vitamin D Variants Levels Exposed and Association With the Disease Severity in Bronchial Asthma

2020 ◽  
Author(s):  
Sultan S Al-Thagfan ◽  
Sameh Ahmed ◽  
Magdy M Emara ◽  
Mohamed Fawzi Awadallah
Keyword(s):  
Vitamin D ◽  
Disease Activity ◽  
Disease Severity ◽  
Bronchial Asthma ◽  
Blood Count ◽  
Immunoglobulin E ◽  
Serum Levels ◽  
Control Group ◽  
Mass System ◽  
Asthmatic Patients

Abstract Background: Several vitamin D variants may be involved in immunity modulation including; vitamin D2 (D2), vitamin D3 (D3), 25-hydroxyvitamin D3 (25(OH) D3) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). The objective of this study is to assess serum levels of vitamin D variants in bronchial asthma patients and their correlations with disease activity markers.Methods: 113 persons divided into two groups were enrolled in this study. The first group included 73 asthmatic patients (57 males and 16 females) and the second included 40 healthy adult (31 male and 9 female) as a control group. Vitamin D variants serum levels were assessed using ultra performance liquid chromatography (UPLC) with tandem mass system. Different disease activity markers were assessed and correlated with serum levels of vitamin D variants. These markers included forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), FEV1/FVC% , peak expiratory flow (PEF), forced expiratory flow25–75% (FEF25-75%), eosinophilic blood count, and total immunoglobulin E (IgE), Results: The study showed that asthmatic patients had significant lower serum levels of vitamin D variants in comparison with the healthy control group (p ≤ 0.001). Also, serum vitamin D variants levels were significantly decreased in uncontrolled asthmatic patients as compared with the partially controlled and controlled patients. The correlation values were higher for 25(OH) D3 and 1,25-(OH) 2D3 compared to D2 and D3. Negative correlations were seen for eosinophilic blood count, total IgE and ACT.Conclusion: Serum levels of all vitamin D variants were reduced in asthmatic patients with moderate to strong correlations to the disease severity. These results suggest that vitamin D deficiency or even insufficiency may play a role in disease severity.

scholarly journals Eosinophil Cationic Protein Concentrations among Crop and Dairy Farmers with Asthma

2018 ◽  
Vol 6 (3) ◽  
pp. 456-462 ◽  
Author(s):  
Saso Stoleski ◽  
Jordan Minov ◽  
Jovanka Karadzinska-Bislimovska ◽  
Dragan Mijakoski ◽  
Aneta Atanasovska
Keyword(s):  
Statistical Significance ◽  
Smoking Habit ◽  
Eosinophil Cationic Protein ◽  
Smoking History ◽  
Control Group ◽  
Cross Sectional Survey ◽  
Dairy Farmers ◽  
Cationic Protein ◽  
Cross Sectional ◽  
The Mean

OBJECTIVE: To assess the mean serum eosinophil cationic protein (s-ECP) concentrations among crop and dairy farmers and office controls, and further examine its relation to exposure duration, smoking habit, as well as presence or absence of asthma.METHODS: A cross-sectional survey was performed including examined group (EG), composed by agricultural workers (87 crop - EG1 and 83 dairy farmers - EG2), and control group (CG) composed by 80 office workers within the same enterprise.  We have used a questionnaire to record the chronic respiratory symptoms, detailed work history, specific farming activities and tasks performed and smoking history. Evaluation of examined subjects also included lung function tests, diagnosis of asthma, and measurement of s-ECP as a marker of inflammation.RESULTS: The main finding of the present study is that s-ECP concentrations were raised in subjects with asthma independent of the smoking habit. The mean s-ECP concentrations were higher in subjects of EG1 and EG2 compared with those in CG, but without reaching statistical significance. Mean s-ECP concentrations were significantly higher among subjects in EG1 exposed more than 20 years, while mean s-ECP concentrations were non-significantly higher in subjects of EG2 exposed more than 20 years, compared to those exposed less than 20 years. Mean s-ECP concentrations were higher among smokers within all three groups, but without reaching statistical significance between smokers and non-smokers. Mean s-ECP concentrations were significantly higher in subjects with asthma within EG1 (P = 0.049) and EG2 (P = 0.040), but also within those in CG (P = 0.046).CONCLUSION: Data obtained suggest that airway inflammation is present in farmers with asthma, and s-ECP is an important biomarker in means of reflecting disease severity and prognosis among exposed workers.

Vitamin D Treatment in Patients with Hashimoto’s Thyroiditis may Decrease the Development of Hypothyroidism

2016 ◽  
Vol 86 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Bekir Ucan ◽  
Mustafa Sahin ◽  
Muyesser Sayki Arslan ◽  
Nujen Colak Bozkurt ◽  
Muhammed Kizilgul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hashimoto’S Thyroiditis ◽  
Healthy Control Group ◽  
Hashimoto's Thyroiditis ◽  
Control Group ◽  
Endocrine Society ◽  
Thyroid Autoantibody ◽  
Healthy Control ◽  
The Mean

Abstract.The relationship between Hashimoto’s thyroiditis and vitamin D has been demonstrated in several studies. The aim of the present study was to evaluate vitamin D concentrations in patients with Hashimoto’s thyroiditis, the effect of vitamin D therapy on the course of disease, and to determine changes in thyroid autoantibody status and cardiovascular risk after vitamin D therapy. We included 75 patients with Hashimoto’s thyroiditis and 43 healthy individuals. Vitamin D deficiency is defined as a 25-hydroxy vitamin D (25(OH)D3) concentration less than 20ng/mL. Vitamin D deficient patients were given 50.000 units of 25(OH)D3 weekly for eight weeks in accordance with the Endocrine Society guidelines. All evaluations were repeated after 2 months of treatment. Patients with Hashimoto’s thyroiditis had significantly lower vitamin D concentrations compared with the controls (9.37±0.69 ng/mL vs 11.95±1.01 ng/mL, p < 0.05, respectively). Thyroid autoantibodies were significantly decreased by vitamin D replacement treatment in patients with euthyroid Hashimoto’s thyroiditis. Also, HDL cholesterol concentrations improved in the euthyroid Hashimoto group after treatment. The mean free thyroxine (fT4) concentrations were 0.89±0.02 ng/dL in patients with Hashimoto’s thyroiditis and 1.07±0.03 ng/dL in the healthy control group (p < 0.001). The mean thyroid volumes were 7.71±0.44 mL in patients with Hashimoto’s thyroiditis and 5.46±0.63 mL in the healthy control group (p < 0.01). Vitamin D deficiency is frequent in Hashimoto’s thyroiditis and treatment of patients with this condition with Vitamin D may slow down the course of development of hypothyroidism and also decrease cardiovascular risks in these patients. Vitamin D measurement and replacement may be critical in these patients.

scholarly journals FRI0342 POST-EFFORT SYNOVIAL AND ENTHESIC RESPONSE IN PATIENTS WITH PSORIATIC ARTHRITIS IN REMISSION OF LOW DISEASE ACTIVITY

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 765.2-765
Author(s):  
C. Guillén-Astete ◽  
P. Zurita-Prada ◽  
C. Urrego-Laurín
Keyword(s):  
Disease Activity ◽  
Power Doppler ◽  
Control Group ◽  
Healthy Population ◽  
Dominant Hand ◽  
Synovial Joint ◽  
Low Disease Activity ◽  
Post Exercise ◽  
Eular Recommendations ◽  
The Mean

Background:Synovial inflammation is part of the pathophysiological process of PsoA although it is considered secondary to enthesitis. It is of interest to assess the synovial joint, peritendinous and enthesic response of patients with PsoA in remission under controlled mechanical stress.Objectives:Our aim is to present our observational experience in patients with APso in remission or low activity of the disease exposed to controlled manual physical efforts adapted to the own grasping capacity of each subject.Methods:Before-after study of a consecutive cohort of patients with PsoA (CASPAR criteria), of at least two years d evolution and DAPSA≤14 at present. Patients with positive rheumatoid factor, patients with exclusively axial forms and patients on biological therapy at the beginning of the study were excluded. All patients underwent controlled basal ultrasound and post-dynamometric exercise (CAMRY EH101-17) of the dominant hand which included the carpus, MCFs, IFPs and IFDs of the 2nd to 5th fingers. The ultrasound findings were scored according to EULAR recommendations in grey scale (GS) and power Doppler (PD) for synovitis, enthesitis and tenosynovitis (maximum scores 71 and 87, respectively). For statistical analysis, comparisons were made with the results of their baseline and post-exercise ultrasound scores between subjects diagnosed with PsoA and controls. The Student’s T test was used for related and unrelated data according to correspondence.Results:Nineteen patients and controls were included, of which 73.7% were male. Mean age: 42.2 SD 6.6 and 42.21 SD 8.28, respectively. Basal DAPSA among patients: 7.26 SD 4.53. Mean traction strength of patients and controls: 23.8 SD 7.3 and 26.1 SD 6.9 kg, respectively (P=0.336). In the group of patients, the post exercise DAPSA had a mean of 7.52 SD 4.62 (P=0.021, with respect to the basal DAPSA). The mean total GS score in the patient group was 3.94 SD 2.36 and 7.31 SD 3.3, pre- and post-exercise, respectively (P<0.001). The mean total score in the PD study was 0.73 SD 0.73 and 2.57 SD 1.16, respectively (P<0.001). In patients with PsoA there were no detectable enthesic changes. In the control group, no ultrasound changes were statistically significant, although the score for tenosynovitis ranged from 0.1 SD 0.31 to 0.42 SD 0.6 (P=0.055).Conclusion:Patients with APso in clinical remission or low disease activity develop ultrasound changes after controlled exercise. These changes are appreciable in the joint synovium and tendon but not at an enthesic level. Although these changes are also detectable in a healthy population, their prevalence is much lower. Our interpretation is that control of the disease correlates with an absence of enthesic inflammatory activity although synovial susceptibility remains less evident. This reinforces the idea that PsoA is an inflammatory enthesitis with associated arthritis and not its opposite.Disclosure of Interests:None declared

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