GENETIC STRUCTURE OF THE KAZAKH FINE-WOOL SHEEP POPULATION BASED ON MOLECULAR GENETIC MARKERS OF DNA

The modern Kazakh population of sheep of the Kazakh fi newool breed is characterized by the following population-genetic indicators: the average number of alleles (N) – 9.5833, the average heterozygosity (expected, not) – 0.8090, the average heterozygosity (observed, but) – 0.6972, the total expected heterozygosity Ht – 0, 8177 and the individual fi xation index (Fis) – 0.1459. 115 alleles were identifi ed, including 89 typical alleles (with a frequency of more than 0.01), 26 private alleles (with a frequency of less than 0.01), and 68.9 eff ective alleles

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Lobster Fishery Connectivity and Management in Indonesia Waters

Journal of Fisheries Science ◽

10.30564/jfsr.v3i1.2466 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Waluyo Waluyo ◽

Taslim Arifin

Keyword(s):

Genetic Structure ◽

Fishery Management ◽

Marine Organism ◽

Molecular Genetic ◽

Three Dimensional ◽

Geographical Information ◽

Biophysical Model ◽

Complex Life Cycle ◽

Biophysical Modeling ◽

The Individual

The distribution of lobsters in Indonesia waters is very wide, even lobster species in Indonesia are also scattered in the tropical waters of the western Pacific Ocean, the Indian Ocean, Africa to Japanese waters.Indonesia waters are divided into 11 (eleven) Fishery Management Zone (FMZ). Lobsters in Indonesia may come from various water areas, both national and regional waters zone, it’s called the sink population, its spread is influenced by the movement of the current. Lobster seed is nurtured by nature through oceancurrents from Australia, East Indonesia, Japan, then back to Australia. Lobsters have a complex life cycle,where adult lobsters inhabit coral reefs as a place to lay eggs, then hatch into planktonic larvae, and grow up in open seas and carry out diurnal and ontogenetic vertical migrations before returning to nurseries in shallow coastal areas and reefs. coral, as well as habitat by the type of species. Literature research had used at leasttwo methodologies to estimate the distribution and connection sensitivity matrices of marine organism larvae.The two most common approaches are using genetic markers and numerical biophysical modeling. Thus, this research uses molecular genetic techniques to explain the genetic structure of lobster populations using a biophysical model approach that can explain the genetic structure of lobsters, as well as the distribution base on regional oceanographic synthesis data and lobster biology known in Indonesia waters. This model has four components, namely: 1) a benthic module based on a Geographical Information System (GIS) which is a lobster habitat in the spawning and recruitment process, 2) a physical oceanography module containing daily velocity in the form of a three-dimensional hydrodynamic model, 3) a larva biology module that describes larval life history characteristics, and 4) a Lagrangian Stochastic module that tracks the individual trajectories of larvae.

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Analysis of the genetic structure of a population of Lebedyn cattle by microsatellite markers

Regulatory Mechanisms in Biosystems ◽

10.15421/021907 ◽

2019 ◽

Vol 10 (1) ◽

pp. 45-49

Author(s):

V. І. Ladyka ◽

L. M. Khmelnychyi ◽

Y. V. Lyashenko ◽

R. O. Kulibaba

Keyword(s):

Genetic Structure ◽

Microsatellite Markers ◽

Gene Pool ◽

Population Genetic ◽

Molecular Genetic ◽

Cattle Breed ◽

Fixation Index ◽

Experimental Population ◽

Negative Effects ◽

Average Value

Microsatellites – a separate class of molecular genetic markers, are widely used for the study of genetic variability, in particular in populations of animals bred by artificial selection under the influence of microevolutionary processes. The object of study is the gene pool of a population of animals of the Lebedyn cattle breed, which is under threat of extinction. The sample comprised 30 individuals from the farm "Komyshans'ke" in Sumy region. The analysis of population genetic structure was performed using 10 microsatellite loci recommended by FAO-ISAG: ETH225, BM2113, ETH3, BM1818, BM1824, ILSTS006, INRA023, TAGLA053, TAGLA12, ETH10. Amplification products were separated in polyacrylamide gels of different concentrations (5–8%), both native and denaturing. All studied loci were polymorphic. The number of detected alleles per locus ranged from 4 to 8 (on average 5 alleles per locus), the size of which ranged from 115 bp (ETH3) to 307 bp (ILSTS006). The majority of the investigated loci (except ETH3) belonged to valuable informative markers (PIC > 0.5). The most polymorphic TGLA053 (8 alleles), BM2113 (6) and ETH3 (6) loci have been identified. In general, the minimum number of alleles (4) was fixed in 50% loci. The main population genetic parameters for the studied loci have been calculated. The highest values of heterozygosity (He), and effective number of alleles (ne) was characterized for loci BM2113, ILSTS006, TGLA053 and ETH225. With the exception of ETH3 and VM1818 loci, the experimental group of animals is in a state of genetic equilibrium. The average value of the Wright fixation index indicates a tendency to increase in the number of homozygous individuals (inbreeding). Comparative analysis of genetic structure of breeds that have a common origin (Lebedyn (PJSC "Mykhaylivka"), Ukrainian grey (DPDG "Polyvanivka"), Red steppe (DPPR "Stepne"), etc.) has been carried out. The obtained results give grounds to assert that in the experimental population of the Lebedyn breed there are processes that lead to a decrease in genetic diversity. In order to overcome the negative effects of artificial reproduction in the gene pool of small populations of cattle, which include Lebedyn cattle, it is appropriate to use microsatellite markers in the selection and breeding work.

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Genetic Diversity and Population Genetic Structure of Erythrophleum fordii Oliv., an Endangered Rosewood Species in South China

Forests ◽

10.3390/f9100636 ◽

2018 ◽

Vol 9 (10) ◽

pp. 636 ◽

Cited By ~ 3

Author(s):

Jing Tan ◽

Zhi-Gang Zhao ◽

Jun-Jie Guo ◽

Chun-Sheng Wang ◽

Jie Zeng

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Endangered Species ◽

Genetic Differentiation ◽

South China ◽

Population Genetic Structure ◽

Population Genetic ◽

The Mean ◽

Erythrophleum Fordii ◽

Private Alleles

Erythrophleum fordii Oliv. is a valuable rosewood species indigenous to the tropical and warm sub-tropical zones of Vietnam, Laos, and South China. The natural forests have been heavily fragmented mostly due to over-exploitation and over-utilization, and alteration to croplands and fast-growing plantations. Therefore, it has been included in the IUCN Red List of Endangered Species as an endangered species. In the present study, genetic diversity and population genetic structure of 11 populations were estimated by SSR makers in South China. Five high polymorphic loci were studied with a total of 34 alleles, among which, seven were private alleles. The mean number of alleles per locus (A), the mean number of efficient alleles per locus (Ae), the observed (Ho) and expected (He) heterozygosity, and Shannon’s index (I) of the 11 populations were 3.40, 2.31, 0.52, 0.56, and 0.90, respectively. Correlation analysis between genetic parameters and geographical factors showed that He and I were in significant negative correlation with longitude, indicating that genetic diversity of E. fordii reduced gradually from West to East in south China. FIS of eight populations with above five samples was on average 0.01, most loci conformed to Hardy-Weinberg equilibrium in these populations; their genetic differentiation coefficient (FST) was 0.18, indicating that genetic differentiation among populations was relatively low and there existed 18% genetic variation among populations. Gene flow (Nm) between these populations was 1.28. The Mantel test showed that genetic distance was not significantly correlated with geographical distance (p > 0.05). It was concluded that populations with high genetic diversity or private alleles, especially Longmen, Wuming and Pingxiang populations should be a priority for in situ conservations, meanwhile more populations and as many families as possible in each population should be collected for ex situ conservations of germplasm resources of this species in the future.

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ASSESSMENT OF THE GENETIC VARIABILITY OF DIFFERENT AGE GROUPS OF SILVER AND BIGHEAD CARPS BY BIOCHEMICAL POLYMORPHISM

Animal Breeding and Genetics ◽

10.31073/abg.61.16 ◽

2021 ◽

Vol 61 ◽

pp. 146-154

Author(s):

I. Stetsiuk ◽

N. Borysenko ◽

T. Nahorniuk ◽

A. Mariutsa

Keyword(s):

Cluster Analysis ◽

Genetic Structure ◽

Genetic Variability ◽

Silver Carp ◽

Age Groups ◽

Bighead Carp ◽

Average Heterozygosity ◽

Blood Samples ◽

Enzyme Systems ◽

Expected Heterozygosity

Goal. The study aimed to analyze and assess the genetic variability of different age groups of silver and bighead carps by the polymorphism of protein and enzyme systems. Methods. Blood samples were taken from silver (Hypophthalmichthys molitrix) and bighead (Aristichthys nobilis) carps of "Sunpoint Ukraine" LLC, Slobozhanske, Zmiiv district, Kharkiv region. Blood from the tail vein was collected from age-2 and age-3 fish in vivo and placed into tubes with an anticoagulant. Whole blood samples were separated into fractions by centrifugation and as a result plasma and erythrocytes were packed into separate tubes. The methods of vertical polyacrylamide and horizontal starch electrophoresis were used for the electrophoretic separation of proteins and enzymes of silver and bighead carps blood. Histochemical staining of the gel plates was carried out with t) and bighead he following genotyping of allelic variants of protein and enzyme systems. Biochemical systems such as loci of prealbumin (Pralb), esterase (EST, K.F. 3.1.1.1), malate dehydrogenase (MDH, K.F. 1.1.1.37), enzyme malic (ME, K.F. 1.1.1.40) and carbonic anhydrase (CA, K.F. 4.2.1.1) were investigated. Statistical processing of experimental data was performed using Biosys-1. Cluster analysis was carried out using MEGA-X. Results. Analysis of the peculiarities of the distribution of allele frequencies and the genotypic composition of Pralb, EST, MDH, ME, and CA loci was carried out in the age-2 and age-3 groups of silver and bighead carps. The prevalence of the frequency of the fast migrating allelic variant F was revealed in comparison with the slow migrating gene at EST locus in the age-3 group of silver carp (Est F = 0.620). The prevalence of the frequency of the fast allelic variant Pralb A in comparison with the slow Pralb B was observed in the silver carp groups (Pralb A = 0.812 in the age-2 and Pralb A = 0.726 in the age-3 groups. According to the genotypic composition of the studied loci, there was an excess of heterozygous individuals in the age-2 group of silver carp, which indicates their genetic imbalance. The formation of breeding stocks of silver and bighead carps requires control of the level of genetic variability of different age groups to monitor changes in the genetic structure and maintain the optimal level of heterozygosity in stocks. The highest level of average heterozygosity (79.4%) was found in the age-2 group of silver carp, which prevailed over the expected average heterozygosity with a value of 49.4%. In comparison with other studied groups, the age-2 group of bighead carp had the lowest level of average heterozygosity of 59.1% versus the theoretically calculated level of 45.5%. The age-2 and age-3 groups of bighead carp had the highest level of heterozygosity at Ca locus at the level of 82.8 and 78.6% respectively, in contrast to the expected heterozygosity at this locus at the level of 49.9 and 49.7%. A dendrogram of the genetic relationships of different age groups of silver carp was constructed based on the values of genetic distances. The formed clusters indicated the formation of the genetic structure of the silver and bighead carps by species affiliation. Conclusions. The analysis of the genetic structure of different age groups of the silver and bighead carps was performed by the polymorphism of protein and enzyme systems such as Pralb, EST, MDH, ME, CA loci. The study describes the features of the distribution of allelic frequencies and genotypic variants of the aforementioned loci. The level of average heterozygosity, observed and expected heterozygosity at loci in age-2 and age-3 groups of silver and bighead carps were determined. The high genetic variability of an age-2 group of silver carp at the level of 79.4% indicates the need to stabilize their genetic structure. As a result of the cluster analysis, it was determined that the formation of the genetic structure of the silver carp groups of the "Sunpoint Ukraine" farm in the Kharkiv region occurs by species affiliation. The study and use of biochemical markers will allow differentiating stocks of silver and bighead carps. As well as in complex analysis with other molecular genetic markers will allow them to develop a genetic passport.

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