scholarly journals The effect of acute exposure to herbicide Gardoprim Plus Gold 500 SC on haematological and biochemical indicators and histopathological changes in common carp (Cyprinus carpio L.)

2011 ◽  
Vol 80 (4) ◽  
pp. 359-363 ◽  
Author(s):  
Radka Dobšíková ◽  
Jana Blahová ◽  
Helena Modrá ◽  
Miša Škorič ◽  
Zdeňka Svobodová
Keyword(s):  
Lactate Dehydrogenase ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Aspartate Aminotransferase ◽  
Cell Function ◽  
Histopathological Examination ◽  
Control Fish ◽  
Histopathological Changes ◽  
Haematological Profile ◽  
Biochemical Indices

The study was focused on the assessment of effects of herbicide preparation Gardoprim Plus Gold 500 SC (terbuthylazine and S-metolachlor as active substances) on haematological and biochemical indices as well as tissue histopathological changes in common carp (Cyprinus carpio L.). Forty eight one- to two-year-old fish were divided into two groups, i.e. 24 fish were treated with 13.0 mg·l-1 of Gardoprim Plus Gold 500 SC and 24 fish were used in control. The experiment was conducted according to OECD method No. 203 Fish, Acute Toxicity Test. Experimental carp showed a significant decrease in leukocyte and lymphocyte counts (P < 0.01) and haematocrit value (P < 0.05) in haematological profile. In biochemical indices, a significant increase in glucose, aspartate aminotransferase, ammonia (P < 0.01), and lactate dehydrogenase (P < 0.05) and a significant decrease in inorganic phosphorus, triglycerides (P < 0.01), and chlorides (P < 0.05) were found in exposed carp compared to control. Histopathological examination revealed lesions in gills and liver. The decline in both leukocyte and lymphocyte counts indicates decreased nonspecific immunity of treated common carp. Increase in ammonia and glucose concentrations, and in catalytic activities of lactate dehydrogenase and aspartate aminotransferase can be related to stress burden, and alteration of liver cell function, respectively, in experimental carp compared to control fish. The study uniquely contributes to the evaluation of the effect of two-component herbicide preparation on common carp.

The effect of praziquantel applied per os on selected haematological and biochemical indices in common carp (Cyprinus carpio L.)

2008 ◽  
Vol 35 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Eliška Sudová ◽  
Veronika Piačková ◽  
Hana Kroupová ◽  
Martin Pijáček ◽  
Zdeňka Svobodová
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Biochemical Indices

scholarly journals The Effect of Mycotoxin Deoxynivalenol on Haematological and Biochemical Indicators and Histopathological Changes in Rainbow Trout (Oncorhynchus mykiss)

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Iveta Matejova ◽  
Helena Modra ◽  
Jana Blahova ◽  
Ales Franc ◽  
Petr Fictum ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Histopathological Examination ◽  
Control Group ◽  
Fish Feed ◽  
Histopathological Changes ◽  
Biometric Parameters ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Commercial Feed ◽  
Biochemical Indices

Deoxynivalenol (DON), produced by theFusariumgenus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2 mg/kg feed. The fish(n=40)were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225 μg/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964 μg/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH(P<0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol(P<0.05), and ammonia(P<0.01)was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol.

scholarly journals Survival of common carp carrying Cyca-DAB1*05 post-challenged with Aeromonas hydrophila

2015 ◽  
Vol 13 (2) ◽  
pp. 167
Author(s):  
Laode Muhammad Arsal ◽  
Munti Yuhana ◽  
Sri Nuryati ◽  
, Alimuddin
Keyword(s):  
Major Histocompatibility Complex ◽  
Molecular Marker ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Aeromonas Hydrophila ◽  
Control Treatment ◽  
Control Fish ◽  
Complex Ii ◽  
Histocompatibility Complex ◽  
Blood Profiles

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp <em>Cyprinus carpio </em>infected by <em>Aeromonas hydrophila</em>. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL <em>A. hydrophila</em>. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with <em>A. hydrophila </em>were higher (P&lt;0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish.</p><p class="Default"> </p><p class="Default">Keywords: <em>Cyprinus carpio</em>, <em>Aeromonas hydrophila</em>, Cyca-DAB1*05, molecular marker</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas <em>Cyprinus carpio </em>setelah diinfeksi dengan bakteri <em>Aeromonas hydrophila </em>yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok <em>major histocompatibility complex </em>II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan <em>A. hydrophila </em>secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P&lt;0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Cyprinus carpio</em>, <em>Aeromonas hydrophila</em>, Cyca-DAB1*05, marka molekuler</p><br /><p> </p>

scholarly journals Comparison of some biochemical and mineral indices among Norik breed Muráň Plain type and Hucul breed mares

2015 ◽  
Vol 84 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Ján Pošivák ◽  
Eva Styková ◽  
František Novotný ◽  
Igor Valocký ◽  
Jana Noskovičová ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Biochemical Analysis ◽  
Iron Chloride ◽  
Parasitic Diseases ◽  
Gamma Glutamyltransferase ◽  
Significant Difference ◽  
Mineral Profile ◽  
Biochemical Indices

Biochemical analysis in horses is an important aid for determining correct clinical diagnosis of general, infectious, and some parasitic diseases. This work studied the biochemical and mineral indices in mares of two breeds: the Norik breed Muráň Plain type and the Hucul breed. A total of 34 mares of the Norik breed Muráň Plain type (aged 15.18 ± 5.99 years) and 28 Hucul mares (aged 9.03 ± 5.50 years) were used. Blood serum was analysed using the biochemical analyser Cobas c111 (Roche, Switzerland). Significant difference (P < 0.05) was found between the Norik breed Muráň Plain type and the Hucul mares in aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and gamma-glutamyltransferase activity; significant difference (P < 0.01) was found in urea values; and highly significant difference (P < 0.001) was found in glucose values. The mineral profile elements showed a highly significant differences (P < 0.001) between the Norik breed Muráň Plain type and the Hucul mares in phosphorus, magnesium, iron, chloride, potassium, and sodium concentrations. The results confirmed that there are significant differences between horse breeds in some biochemical indices. Therefore, it is appropriate to determine reference values for other horse breeds, as well. To our knowledge, this is the first report that compares biochemical and mineral indices between the Norik breed Muráň Plain type and the Hucul breed.

scholarly journals Effects of clove oil anaesthesia on common carp (Cyprinus carpio L.)

2012 ◽  
Vol 50 (No. 6) ◽  
pp. 269-275 ◽  
Author(s):  
J. Velisek ◽  
Z. Svobodova ◽  
V. Piackova ◽  
L. Groch ◽  
L. Nepejchalova
Keyword(s):  
Acute Toxicity ◽  
Common Carp ◽  
Clove Oil ◽  
Irreversible Damage ◽  
Fish Exposure ◽  
Haematological Profile ◽  
Gill Filaments ◽  
Biochemical Profiles ◽  
Biochemical Indices ◽  
And Control

The aim of the study was to investigate acute toxicity of clove oil for common carp and, using values of haematological and biochemical profiles of blood and histological tissue examinations, to assess the effects of the fish exposure to that anaesthetic. Acute toxicity values of clove oil for carp were found 10 minLC50 74.3&nbsp;mg/l; 10minLC0.1 51.6 mg/l; 10minLC99.9 110.1 mg/l; 96hLC50 18.10 mg/l; 96hLC0.1 15.45 mg/l; and 96hLC99.9 19.80&nbsp;mg/l. The fish were divided into four groups for haematological and biochemical examinations of blood and histological examinations of tissues. The groups were Control I (before the anaesthetic administration), Experiment I (immediately after 10 min anaesthesia at the concentration of 30 mg/l), Experiment II (24 hrs after 10 min anaesthesia) and Control II (controls examined in parallel with Experiment II). A total of 40 carp were examined. Clove oil anaesthesia had not effect on the haematological profile. The 10-min exposure to clove oil at a concentration of 30 mg/l caused a significant (P &lt; 0.01) increase in the concentration of glucose (GLU) and inorganic phosphate (PHOS) immediately after anaesthesia. Clove oil anaesthesia had not effect on other biochemical indices. Histological examination showed capillary ectasia of gill filaments immediately after clove oil anaesthesia. Twenty-four hours after anaesthesia, no ectasia was observed. No histopathological changes were demonstrated in other tissues following anaesthesia. Results of the examinations suggest that the use of clove oil at a concentration of 30 mg/l does not cause irreversible damage in carp.

scholarly journals Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture

2013 ◽  
Vol 8 (10) ◽  
pp. 975-985 ◽  
Author(s):  
Božidar Rašković ◽  
Ivan Jarić ◽  
Vesna Koko ◽  
Milan Spasić ◽  
Zorka Dulić ◽  
...  
Keyword(s):  
Water Quality ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Stream Water ◽  
Histopathological Changes ◽  
Water Supplies ◽  
Common Carp Cyprinus Carpio ◽  
Sensitive Organ ◽  
Carp Cyprinus Carpio ◽  
Tube Well

AbstractIn order to evaluate the relationship between water quality in ponds and indices of histopathological changes occurring in the vital organs of the common carp (Cyprinus carpio L., 1758), two six-month field experiments were carried out using two different water supplies: from the nearby stream and a tube well. The fish were fed supplemental feed: raw cereals, pelleted and extruded compound feed. Histopathological analysis, alteration frequencies, and semi-quantitative scoring of the changes were used to assess the health status of the fish. Ponds supplied by stream water were characterized by higher water hardness, dissolved oxygen and pH values, while those supplied by the tube well had higher electroconductivity, total ammonium and orthophosphates content. Fish survival rate and habitat suitability index were lower in ponds supplied by stream water, while the weight gain did not differ between the two water supplies. The use of stream water resulted in a higher level of histopathological changes in gills and liver. Among the water quality parameters, pH level had the strongest influence on fish. Differences in water supply produced greater influence on the level of histopathological changes than the type of feed applied. Gills were the most sensitive organ, while the kidney was the least responsive.

scholarly journals Stress Response to Long Distance Transportation of Common Carp (Cyprinus carpio L.)

2006 ◽  
Vol 75 (3) ◽  
pp. 437-448 ◽  
Author(s):  
R. Dobšíková ◽  
Z. Svobodová ◽  
J. Blahová ◽  
H. Modrá ◽  
J. Velíšek
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Stress Responses ◽  
Haemoglobin Concentration ◽  
Long Distance ◽  
Corpuscular Haemoglobin ◽  
Dependent Relation ◽  
Haematological Profile ◽  
Leukocyte Counts ◽  
Mean Corpuscular Haemoglobin

The stress responses and changes in biochemical and haematological indices were investigated in three-year-old common carp (Cyprinus carpio L.) during a long-distance transportation in special truck tanks. Twelve-hour transportation caused a significant increase in ammonia (p < 0.01), mean corpuscular volume MCV (p < 0.01), metamyelocytes ( p < 0.05) and band neutrophils (p < 0.01), and a significant decrease in Cl- (p < 0.05), lactate (p < 0.05), ALT (p < 0.05) and ALP (p < 0.01) levels. The values of LDH (p < 0.01), AST (p < 0.05), CK (p < 0.01) and haematocrit PCV (p < 0.05) were also significantly influenced by the transportation, but no time-dependent relation was found. On the contrary, the levels of cortisol, glucose and total protein in the biochemical profile, and the values of erythrocyte count (RBC), haemoglobin (Hb), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), leukocyte counts (WBC) and leukogram (except for metamyelocytes and band neutrophils) in the haematological profile were not significantly influenced by the transportation. Results showed that pre-transport fish manipulation (hauling, netting, handling, loading) was found to be an important stressor for fish. Long-distance transportation itself was relatively considerate for the common carp tested.

Sign in / Sign up

Export Citation Format

Share Document