scholarly journals Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection

2015 ◽  
Vol 20 (41) ◽  
Author(s):  
Sang Won Park ◽  
Ranawaka A P M Perera ◽  
Pyoeng Gyun Choe ◽  
Eric H Y Lau ◽  
Seong Jin Choi ◽  
...  
Keyword(s):  
Middle East ◽  
Immunosorbent Assay ◽  
Symptom Onset ◽  
Middle East Respiratory Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Course Of Illness ◽  
Antigenic Similarity ◽  
Antibody Titres ◽  
Coronavirus Infection ◽  
Time Periods

Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.

scholarly journals Current understanding of middle east respiratory syndrome coronavirus infection in human and animal models

2018 ◽  
Vol 10 (S9) ◽  
pp. S2260-S2271 ◽  
Author(s):  
Yanqun Wang ◽  
Jing Sun ◽  
Airu Zhu ◽  
Jingxian Zhao ◽  
Jincun Zhao
Keyword(s):  
Middle East ◽  
Animal Models ◽  
Middle East Respiratory Syndrome ◽  
Current Understanding ◽  
Coronavirus Infection

scholarly journals Analysis ofLeishmaniamimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Parasitology ◽  
2018 ◽  
Vol 145 (14) ◽  
pp. 1938-1948 ◽  
Author(s):  
Lígia Moraes Barizon de Souza ◽  
Vanete Thomaz Soccol ◽  
Ricardo Rasmussen Petterle ◽  
Michelle D. Bates ◽  
Paul A. Bates
Keyword(s):  
Cell Surface ◽  
Cutaneous Leishmaniasis ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cell Surfaces ◽  
Cell Markers ◽  
Antibody Titres

AbstractOligosaccharides are broadly present onLeishmaniacell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβis the immunodominant saccharide inLeishmaniacell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P< 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

scholarly journals Factors Associated With Viral Load Kinetics of Middle East Respiratory Syndrome Coronavirus During the 2015 Outbreak in South Korea

2020 ◽  
Author(s):  
Jeong-Sun Yang ◽  
Min-Gyu Yoo ◽  
Hye-Ja Lee ◽  
Han Byul Jang ◽  
Hee-Dong Jung ◽  
...  
Keyword(s):  
Viral Load ◽  
Middle East ◽  
Symptom Onset ◽  
Healthcare Workers ◽  
Middle East Respiratory Syndrome ◽  
Viral Loads ◽  
Hospitalization Period ◽  
Using Data ◽  
Kinetics Of ◽  
Respiratory Specimens

Abstract We conducted a retrospective study of Middle East respiratory syndrome coronavirus (MERS-CoV) viral load kinetics using data from patients hospitalized with MERS-CoV infection between 19 May and 20 August 2015. Viral load trajectories were considered over the hospitalization period using 1714 viral load results measured in serial respiratory specimens of 185 patients. The viral load levels were significantly higher among nonsurvivors than among survivors (P = .003). Healthcare workers (P = .001) and nonspreaders (P &lt; .001) had significantly lower viral loads. Viral RNA was present on the day of symptom onset and peaked 4–10 days after symptom onset.

scholarly journals Passive Immunotherapy with Dromedary Immune Serum in an Experimental Animal Model for Middle East Respiratory Syndrome Coronavirus Infection

2015 ◽  
Vol 89 (11) ◽  
pp. 6117-6120 ◽  
Author(s):  
Jincun Zhao ◽  
Ranawaka A. P. M. Perera ◽  
Ghazi Kayali ◽  
David Meyerholz ◽  
Stanley Perlman ◽  
...  
Keyword(s):  
Middle East ◽  
Pulmonary Infection ◽  
Serum Antibody ◽  
Case Fatality ◽  
Immune Serum ◽  
Middle East Respiratory Syndrome ◽  
Specific Therapy ◽  
Passive Immunotherapy ◽  
Coronavirus Infection ◽  
Kinetics Of

ABSTRACTMiddle East respiratory syndrome (MERS) is a highly lethal pulmonary infection. Serum from convalescent MERS patients may provide some benefit but is not readily available. In contrast, nearly all camels in the Middle East have been infected with MERS-CoV. Here, we show that sera obtained from MERS-immune camels augment the kinetics of MERS-CoV clearance and reduce the severity of pathological changes in infected lungs, with efficacy proportional to the titer of MERS-CoV-neutralizing serum antibody.IMPORTANCEMiddle East respiratory syndrome, caused by a coronavirus, is highly lethal, with a case fatality rate of 35 to 40%. No specific therapy is available, and care is generally supportive. One promising approach is passive administration of sera from convalescent human MERS patients or other animals to exposed or infected patients. The vast majority of, if not all, camels in the Middle East have been infected with MERS-CoV, and some contain high titers of antibody to the virus. Here, we show that this antibody is protective if delivered either prophylactically or therapeutically to mice infected with MERS-CoV, indicating that this may be a useful intervention in infected patients.

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