scholarly journals Monitoring West Nile virus (WNV) infection in wild birds in Serbia during 2012: first isolation and characterisation of WNV strains from Serbia

2013 ◽  
Vol 18 (44) ◽  
Author(s):  
T Petrović ◽  
A B Blázquez ◽  
D Lupulović ◽  
G Lazić ◽  
E Escribano-Romero ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Migratory Birds ◽  
Genomic Sequence ◽  
Wild Birds ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Tissue Samples ◽  
Full Genomic Sequence ◽  
Common Pheasant ◽  
Mute Swans

West Nile virus (WNV), a neurovirulent mosquito-transmissible zoonotic virus, has caused recent outbreaks in Europe, including Serbia from August until October 2012. Although humans can be infected, birds are the main natural WNV reservoir. To assess WNV circulation in northern Serbia, 133 wild birds were investigated. These comprised resident and migratory birds, collected between January and September 2012 in the Vojvodina province. The birds belonged to 45 species within 27 families. Blood sera (n=92) and pooled tissues from respective birds (n=81) were tested by enzyme-linked immunosorbent assay (ELISA), plaque reduction neutralisation test (PRNT) and real-time reverse transcription-polymerase chain reaction (RT-qPCR). WNV antibodies were detected in seven (8%) sera: four from Mute Swans (Cygnus olor), two from White-tailed Eagles (Haliaeetus albicillas), and one from a Common Pheasant (Phasianus colchicus). Five sera neutralised WNV but not Usutu virus. For the first time in Serbia, WNV RNA was detected by RT-qPCR in pooled tissue samples of eight respective birds. WNV RNA was also derived from an additional bird, after a serum sample resulted infective in cell culture. The total nine WNV RNA positive birds included three Northern Goshawks (Accipiter gentilis), two White-tailed Eagles, one Legged Gull (Larus michahelis), one Hooded Crow (Corvus cornix), one Bearded Parrot-bill (Panarus biramicus), and one Common Pheasant. Phylogenetic analysis of partial E region sequences showed the presence of, at least, two lineage 2 Serbian clusters closely related to those responsible for recent human and animal outbreaks in Greece, Hungary and Italy. Full genomic sequence from a goshawk isolate corroborated this data. These results confirm WNV circulation in Serbia and highlight the risk of infection for humans and horses, pointing to the need for implementing WNV surveillance programmes.

scholarly journals Serological Evidence of West Nile Virus in Wild Birds in Bangladesh

2020 ◽  
Vol 7 (4) ◽  
pp. 164
Author(s):  
Ariful Islam ◽  
Shariful Islam ◽  
Mohammad Enayet Hossain ◽  
Jinnat Ferdous ◽  
Josefina Abedin ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Migratory Birds ◽  
Wild Birds ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igg Antibody ◽  
West Nile ◽  
Sylvatic Cycle ◽  
Tufted Duck ◽  
Vector Borne

West Nile Virus (WNV) is a vector-borne zoonotic disease maintained in a sylvatic cycle involving mosquito vectors and birds. To detect WNV and other flavivirus infections in wild resident and migratory birds, we tested 184 samples from 19 identified species within nine families collected during 2012–2016 from four districts in Bangladesh. We tested serum samples for the immunoglobulin G (IgG) antibody against WNV using competitive Enzyme-Linked Immunosorbent Assay (c-ELISA), whereas tracheal and cloacal swabs were subjected to consensus Polymerase Chain Reaction (c-PCR) for the detection of the flavivirus RNA. Overall, we detected 11.9% (n = 22; 95% CI: 0.07–0.16) samples were seropositive, including 15.9% in the migratory wild birds and 10.7% in the resident wild birds. The migratory wild Tufted duck showed 28.5% seropositivity, whereas the resident wild house crows showed 12.5% seropositivity. None of the swab samples was positive for flavivirus RNA infection (0%, n = 184; 95% CI: 0–0.019). These study findings recommend continued surveillance for early detection and to better understand the epidemiology of WNV and other flavivirus circulation in both birds and mosquitoes in Bangladesh.

scholarly journals Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2367
Author(s):  
Mohd Yuseri Ain-Najwa ◽  
Abd Rahaman Yasmin ◽  
Siti Suri Arshad ◽  
Abdul Rahman Omar ◽  
Jalila Abu ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Peninsular Malaysia ◽  
Wild Birds ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mangrove Forests ◽  
Rt Pcr ◽  
Serum Samples ◽  
West Nile ◽  
The Status

The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown that 1:7 and 1:6 Malaysian wild birds are WNV antibody and RNA positive, respectively, and bats in North America may not be susceptible to the WNV infection. This study was conducted to determine the status of WNV in Malaysian macaques and bats found in mangrove forests and caves, respectively. Archive sera and oropharyngeal swabs from long-tailed macaques were subjected to the antibody detection using WNV competitive enzyme-linked immunosorbent assay (c-ELISA) and WNV RNA using RT-PCR, respectively, while the archive oropharyngeal and rectal swabs from bats were subjected to RT-PCR without serological analysis due to the unavailability of serum samples. The analysis revealed a WNV seropositivity of 29.63% (24/81) and none of the macaques were positive for WNV RNA. Meanwhile, 12.2% (5/41) of the bats from Pteropodidae, Emballonuridae, and Rhinolophidae families tested positive for WNV RNA. Here, we show a high WNV antibody prevalence in macaques and a moderate WNV RNA in various Malaysian bat species, suggesting that WNV circulates through Malaysian wild animals and Malaysian bat species may be susceptible to the WNV infection.

A Snapshot Avian Surveillance Reveals West Nile Virus and Evidence of Wild Birds Participating in Toscana Virus Circulation

2017 ◽  
Vol 17 (10) ◽  
pp. 698-708 ◽  
Author(s):  
Sabri Hacioglu ◽  
Ender Dincer ◽  
Cafer Tayer Isler ◽  
Zeynep Karapinar ◽  
Veysel Soydal Ataseven ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Birds ◽  
Toscana Virus ◽  
West Nile

The survey of wild birds for West Nile virus in Poland

2011 ◽  
Vol 14 (4) ◽  
pp. 573-577 ◽  
Author(s):  
J. Niczyporuk ◽  
E. Samorek-Salamonowicz ◽  
W. Kozdruń ◽  
Z. Mizak
Keyword(s):  
West Nile Virus ◽  
Genetic Material ◽  
Early Spring ◽  
Wild Birds ◽  
Rt Pcr ◽  
West Nile ◽  
Late Autumn ◽  
The Brain

The survey of wild birds for West Nile virus in PolandTwo thousand one hundred and forty birds belonging to 39 different species from different locations in Poland were examined. The study has taken place from the early spring till late autumn 2007-2010 when the activity of the mosquitoes was the highest. The brain samples were taken from the birds and whole cellular RNA was isolated, then the RT-PCR and NRT-PCR were performed to detect the presence of West Nile virus (WNV). The obtained results were confirmed by the commercial WNV Kit. No genetic material of WNV was found in the examined samples.

Surveillance on antibodies against West Nile virus, Usutu virus, tick-borne encephalitis virus and Tribeč virus in wild birds in Drienovská wetland, Slovakia

Biologia ◽  
2019 ◽  
Vol 74 (7) ◽  
pp. 813-820 ◽  
Author(s):  
Tomáš Csank ◽  
Ľuboš Korytár ◽  
Terézia Pošiváková ◽  
Tamás Bakonyi ◽  
Juraj Pistl ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Birds ◽  
Encephalitis Virus ◽  
West Nile ◽  
Usutu Virus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

West Nile virus lineage 2 in Sardinian wild birds in 2012: a further threat to public health

2013 ◽  
Vol 141 (11) ◽  
pp. 2313-2316 ◽  
Author(s):  
G. SAVINI ◽  
G. PUGGIONI ◽  
A. DI GENNARO ◽  
G. DI FRANCESCO ◽  
A. M. ROCCHIGIANI ◽  
...  
Keyword(s):  
Public Health ◽  
West Nile Virus ◽  
Sequence Analysis ◽  
Wild Birds ◽  
Homologous Sequence ◽  
West Nile ◽  
Lineage 2 ◽  
High Level

SUMMARYWest Nile virus (WNV) strains belonging to lineage 2 were detected and isolated from the tissues of a goshawk and two carrion crows in Sardinia in August 2012. According to NS3 sequence analysis, the Sardinian isolates shared a high level of similarity with those of Italian lineage 2 strains which circulated in 2011 and with the homologous sequence of the 2004 Hungarian isolate. Following the human fatality reported in 2011 in Olbia, this study is the first to report the spread and enzootic circulation of WNV lineage 2 in Sardinia.

scholarly journals West Nile Virus and Related Flavivirus in European Wild Boar (Sus scrofa), Latium Region, Italy: A Retrospective Study

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 494
Author(s):  
Angela Petruccelli ◽  
Tiziana Zottola ◽  
Gianmarco Ferrara ◽  
Valentina Iovane ◽  
Cristina Di Russo ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Boar ◽  
Specific Antibody ◽  
Central Italy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Serum Samples ◽  
West Nile ◽  
Wild Boars ◽  
European Wild Boar

Background: A retrospective sero-survey for evidence of West Nile virus (WNV) infection in European wild boar (Sus scorfa) was conducted in the Latium region, Italy, on stored serum samples of the period November 2011 to January 2012. Methods: Sera were collected from 168 European wild boars and screened for antibodies to WNV and other Flaviviruses by competitive enzyme linked immunosorbent assay (cELISA). All sera positive for Flavivirus antibodies by cELISA were further examined by virus neutralization test (VNT). To test the presence of Flavivirus RNA in samples, an RT-PCR was performed using a pan-Flavivirus primers pair. Results: Thirteen wild boars (7.73%) were seropositive for Flaviviruses. The hemolysis of serum samples limited the interpretation of the VNT for 7 samples, confirming the presence of specific antibody against WNV in a single European wild boar serum sample. The presence of ELISA positive/VNT negative samples suggests the occurrence of non-neutralizing antibodies against WNV or other antigen-related Flaviviruses. No samples resulted positive for Flavivirus by RT-PCR assay. Conclusion: Although a moderately high percentage of animals with specific antibody for WNV has been detected in wild boar in other surveillance studies in Europe, this has not been reported previously in Italy. Together, these data indicate that European wild boar are exposed to WNV and/or other related-Flavivirus in central Italy and confirm the usefulness of wild ungulates, as suitable Flavivirus sentinels.

Attempts to detect West Nile virus in wild birds in Poland

2011 ◽  
Vol 59 (3) ◽  
pp. 405-408 ◽  
Author(s):  
Jowita Niczyporuk ◽  
Elżbieta Samorek-Salamonowicz ◽  
Wojciech Kozdruń ◽  
Zbigniew Mizak
Keyword(s):  
West Nile Virus ◽  
Early Spring ◽  
Wild Birds ◽  
Rt Pcr ◽  
West Nile ◽  
Late Autumn ◽  
The Brain

The aim of the study was to attempt the detection of West Nile virus (WNV) in wild birds in Poland. Forty-eight species of 1912 wild birds were used for the investigations. The birds were derived from various locations in Poland from early spring till late autumn of the years 2009–2011. The brain samples were homogenised and cellular RNA was isolated. Two methods (RT-PCR and nested RT-PCR) were used. The presence of WNV RNA was not detected in the samples examined. Additionally, a short analysis of the epizootiological situation regarding the presence of WNV in Poland is presented.

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