scholarly journals EXPRESSION PROFILE OF SEX DETERMINATION GENE, BIOREPRODUCTION, PHENOTYPE, AND LOCOMOTORY PERFORMANCES OF OLIVE RIDLEY, Lepidochelys olivacea INDUCED BY DIFFERENT INCUBATION TEMPERATURE

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Alfred O. M. Dima ◽  
Dedy D. Solihin ◽  
Wasmen Manalu ◽  
Arief Boediono
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Incubation Period ◽  
Incubation Temperature ◽  
Development Stage ◽  
Sensitive Period ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Student’S T ◽  
Student’S T Test

<p><em>S</em><em>ex determination </em><em>in turtle species </em><em>is not only based on genotype, but also rely on the incubation temperature. In addition, sexual differentiation takes place during the thermo-sensitive period (TSP). This study was conducted to determine the effects of incubation temperature on </em><em>sex </em><em>expression profile of determination gene, bioreproduction, </em><em> phenotype</em><em>, and locomotory performances  of olive ridley turtle hatchlings. Fertile eggs incubated at two temperatures, namely feminine temperature (30-33°C), and masculine temperature (26-27°C). Value of cycle threshold (C<sub>T</sub>) measured during TSP, i.e 23-25 embryonic development stage, and after TSP, i.e 26-27 embryonic development stage using real time PCR techniques. Comparison of gene expression at both incubation temperatures were analyzed by ANOVA, and Student’s t test. Hatchling bioreproduction and phenotype measurement consist of the incubation period, embryo growth, morphometrics, and locomotori performances hatchlings were analyzed with regression analysis and Student’s t test. The results showed expression of both aromatase and Rspond 1 genes (which plays a role in ovarian differentiation) after the TSP that incubated at feminine temperature higher and different with masculine temperature. In conjunction with the  bioreproduction and phenotype, the incubation period of feminine temperature shorter than that of masculine. Likewise, growth of the embryo of feminine temperature was faster than that of masculine. Incubation at feminine temperature significantly affect to carapace width, length and width of the plastron, long flippers and rear arms, long neck, and the frequency of the swing flippers.</em><em> </em></p> <strong><em>Keywords: </em></strong><em>thermo-sensitive period (TSP), gene expression, phenotype, Lepidochelys olivacea</em>

scholarly journals EXPRESSION PROFILE OF SEX DETERMINATION GENE, BIOREPRODUCTION, PHENOTYPE, AND LOCOMOTORY PERFORMANCES OF OLIVE RIDLEY, Lepidochelys olivacea INDUCED BY DIFFERENT INCUBATION TEMPERATURE

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Alfred O. M. Dima ◽  
Dedy D. Solihin ◽  
Wasmen Manalu ◽  
Arief Boediono
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Incubation Period ◽  
Incubation Temperature ◽  
Development Stage ◽  
Sensitive Period ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Student’S T ◽  
Student’S T Test

Sex determination in turtle species is not only based on genotype, but also rely on the incubation temperature. In addition, sexual differentiation takes place during the thermo-sensitive period (TSP). This study was conducted to determine the effects of incubation temperature on sex expression profile of determination gene, bioreproduction, phenotype, and locomotory performances  of olive ridley turtle hatchlings. Fertile eggs incubated at two temperatures, namely feminine temperature (30-33°C), and masculine temperature (26-27°C). Value of cycle threshold (CT) measured during TSP, i.e 23-25 embryonic development stage, and after TSP, i.e 26-27 embryonic development stage using real time PCR techniques. Comparison of gene expression at both incubation temperatures were analyzed by ANOVA, and Student’s t test. Hatchling bioreproduction and phenotype measurement consist of the incubation period, embryo growth, morphometrics, and locomotori performances hatchlings were analyzed with regression analysis and Student’s t test. The results showed expression of both aromatase and Rspond 1 genes (which plays a role in ovarian differentiation) after the TSP that incubated at feminine temperature higher and different with masculine temperature. In conjunction with the  bioreproduction and phenotype, the incubation period of feminine temperature shorter than that of masculine. Likewise, growth of the embryo of feminine temperature was faster than that of masculine. Incubation at feminine temperature significantly affect to carapace width, length and width of the plastron, long flippers and rear arms, long neck, and the frequency of the swing flippers. Keywords: thermo-sensitive period (TSP), gene expression, phenotype, Lepidochelys olivacea

26 Drugs that Modify Epigenetics...What do they do to Porcine Clones?

2018 ◽  
Vol 30 (1) ◽  
pp. 152
Author(s):  
C. P. Buemo ◽  
A. Gambini ◽  
L. N. Moro ◽  
N. Canel ◽  
D. F. Salamone
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Electric Pulse ◽  
Donor Cell ◽  
T Test ◽  
Control Group ◽  
Developmental Rates ◽  
Student’S T ◽  
Student’S T Test

Although somatic cell nuclear transfer (SCNT) technology was developed more than 20 years ago, cloning efficiency remains low. Failures in the reprogramming of the donor cell result in embryos with aberrant epigenetic patterns and low developmental rates. In this study, we assessed whether the use the inhibitor of DNA (cytosine 5) methyltransferase 5-azacitidine (5Aza) combined with the MEK inhibitor in the MAPK pathway PD0325901 (PD) could improve SCNT efficiency in pigs. In vitro maturation of cumulus–oocyte complexes was performed in TCM for 44 h at 39°C under 5% CO2. Cumulus cells and zona pellucida was removed from matured oocytes, followed by enucleation of the metaphase plate previously stained with Hoëchst 33342. Each enucleated oocyte was attached to a donor cell by phytohemagglutinin treatment followed by an electric pulse of 80V for 30 μs. After fusion, reconstituted embryos were activated by an electric pulse followed by an incubation in 2 mM 6-DMAP for 3 h. Cloned embryos were cultured in vitro in a modified well-of-well system in SOF medium, where 3 cloned embryos were placed per microwell (3X). The experimental group 3X + drugs was exposed for the first 3 days to 1 μM PD and 1 μM 5Aza in SOF medium. After washing, embryos were cultured until Day 7 in regular SOF medium. The control group (3X) was cultured in regular SOF medium for 7 days. In vitro embryo developmental rates, gene expression, histone acetylation, and DNA methylation status were studied. The use of epigenetic modifying drugs significantly increased blastocyst rates (40.9% v. 29%; Fisher’s test, P < 0.05) and embryo size (41.46% v. 28.56%; Student’s t-test, P < 0.05) compared with the control group. Regarding gene expression, an increase of the relative expression of genes related to cell differentiation (Igf2 and Cdx2), antiapoptotic pathways (Bcl-xl) and DNA methylation modulation (Mapk1) was observed (P < 0.05). Pluripotency genes Oct4 and Nanog did not show differences between groups. The Bax proapoptotic gene significantly decreased its expression after drug treatment, as did the Klf4 gene (P < 0.05). Results were analysed by Student’s t-test. According to Histone H3K27ac, which is associated with enhancers or gene promoters, its marker was located mainly in the nuclear periphery respect to the control group with a uniform dispersion, indicating that the treatment could be activating certain genes by locating them near the periphery. Histone H3K4me1 was more uniformly localised throughout the nucleus in both groups. The intensity of the fluorescence was measured by quantitative confocal microscopy using a histogram produced by the ImageJ program (National Institutes of Health, Bethesda, MD, USA). Regarding DNA methylation by bisulphite sequencing, the 2 genes studied (Oct4 and DNMT1) showed a higher demethylation status for the treated group. Our results indicate that the combination of 5Aza+PD during early pre-implantation development dramatically increase blastocyst rates and embryo quality. This novel combination could be used as a strategy to improve the efficiency of SCNT in pigs and potentially other animals.

Potentiated 1,25(OH)2D3-induced 24-hydroxylase gene expression in uremic rat intestine

1994 ◽  
Vol 267 (6) ◽  
pp. F926-F930 ◽  
Author(s):  
H. Koyama ◽  
M. Inaba ◽  
Y. Nishizawa ◽  
E. Ishimura ◽  
Y. Imanishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dot Blot ◽  
Rat Intestine ◽  
Dihydroxyvitamin D3 ◽  
Calbindin D9k ◽  
Hydroxylase Gene ◽  
Poorly Differentiated ◽  
Student’S T ◽  
Student’S T Test

24-Hydroxylase has been considered a major enzyme regulating metabolism of circulating 1 alpha, 25-dihydroxyvitamin D3 [1,25(OH)2D3]. To understand the metabolism of 1,25(OH)2D3 in chronic renal failure, we examined 1,25(OH)2D3-induced 25-hydroxyvitamin D3-24-hydroxylase (24-hydroxylase) gene expression in the intestine of uremic rats. Northern blot and dot blot analyses showed that the induction of duodenal 24-hydroxylase gene expression was 2.0- to 3.8-fold greater in uremic rats than in sham-operated rats (P < 0.05, Student's t-test) at 6 h after 1,25(OH)2D3 administration. Gene induction of calbindin D9k by 1,25(OH)2D3 was not augmented in uremic group. In situ hybridization analysis revealed that the induction of 24-hydroxylase mRNA by 1,25(OH)2D3 was observed exclusively in the columnar epithelium of the crypt and the lower part of the villi, suggesting that the stage of epithelial cell differentiation is a major determinant of 1,25(OH)2D3-induced 24-hydroxylase gene expression. In uremia, 1,25(OH)2D3-induced 24-hydroxylase gene expression was accelerated selectively, possibly because of poorly differentiated epithelial cells.

scholarly journals Field lethal incubation temperature of olive ridley sea turtle Lepidochelys olivacea embryos at a mass nesting rookery

2010 ◽  
Vol 12 (1) ◽  
pp. 77-86 ◽  
Author(s):  
RA Valverde ◽  
S Wingard ◽  
F Gómez ◽  
MT Tordoir ◽  
CM Orrego
Keyword(s):  
Incubation Temperature ◽  
Sea Turtle ◽  
Olive Ridley ◽  
Lepidochelys Olivacea

scholarly journals Suhu inkubasi, pasir pantai peneluran dan sukses penetasan telur penyu pada sarang semi alami di Pantai Warebar - Yenbekaki Distrik Waigeo Timur, Kabupaten Raja Ampat

Cassowary ◽  
2020 ◽  
Vol 3 (1) ◽  
pp. 21-31
Author(s):  
Aditya P. Tarigan ◽  
Ricardo F. Tapilatu ◽  
Marthin Matulessy
Keyword(s):  
Incubation Temperature ◽  
Field Research ◽  
Sea Turtle ◽  
Hatching Rate ◽  
Nest Temperature ◽  
Olive Ridley ◽  
Eretmochelys Imbricata ◽  
Turtle Species ◽  
Lepidochelys Olivacea ◽  
Sand Temperature

The research took place between May and October 2019, and divided into two stages. The first stage was field research to collect data by identifying turtles, calculating hatching successes, measuring the temperature of the nesting beach and nest temperature of each species of turtle in semi-natural nests at Warebar Beach, Yenbekaki village, East Waigeo Sub District, Raja Ampat. The second stage after the field was conducting an analysis of hatching rate in hatchery, sand temperature and nest temperature for each species of turtle. The species of turtles nesting at Warebar Beach are olive-ridley  (Lepidochelys olivacea) and hawksbill (Eretmochelys imbricata) turtles. Based on the measurement results at semi-natural nests, it was obtained that the hatching rate of olive ridley sea turtle was 71.6%±28.3 (X±SD) and hawksbill sea turtle was 59.8%±41.3, the overall mean temperature of nesting beach was 28oC, the mean incubation temperature for both sea turtle species was 31oC.

scholarly journals The Effect of Thermal Conditioning on Thyroid Hormones, Hatchability and Embrionic Mortality of Broilers during the Incubation Period

2017 ◽  
Vol 66 (1-2) ◽  
pp. 32-37
Author(s):  
Zoran Ružić ◽  
Zdenko Kanački ◽  
Dragan Žikić ◽  
Gordana Ušćebrka
Keyword(s):  
Thyroid Gland ◽  
Embryonic Development ◽  
Incubation Period ◽  
Incubation Temperature ◽  
Embryonic Mortality ◽  
Control Group ◽  
Free T4 ◽  
Hormone Levels ◽  
Hatching Percentage ◽  
Positive Effect

SummaryThe aim of this study was to examine the effect of thermal conditioning on the embryonic mortality, on the time of embryonic mortality, as well as to examine the effect of this temperature regime on hatching and on thyroid gland hormone levels. 400 fertile eggs, randomly divided into two groups with two repetitions, were used in this research. The control group was exposed to the standard temperature (37.8°C) through the whole embryonic development. The second group was incubated under the standard conditions as the control group, up to 15thday of embryonic development. During 16th, 17thand 18thday of embryonic development, the incubation temperature was increased to 39°C for 3 hours. From 19thday up to the end of the incubation period the conditions of incubation were identical to those of the control group. Results of this study show that the applied treatment leads to an increase of concentration of free T3 hormone on the day 19 of embryonic development, as opposed to a decrease of free T4 hormone in the embryo’s blood compared to the control group. The percentage of hatchability was 82.2% in the control group and 83.3% in the second group. The result shows that this way of thermal manipulation did not affect the embryonic mortality, which was 9.5% in the control group and 8.9% in the experimental group. It can be concluded that in this manner thermal conditioning may manifest the positive effect on the thyroid gland hormone levels without increasing embryonic mortality or disturbing the hatching percentage.

Serosal and cutaneous recordings of gastric myoelectrical activity in patients with gastroparesis

1994 ◽  
Vol 266 (1) ◽  
pp. G90-G98 ◽  
Author(s):  
J. D. Chen ◽  
B. D. Schirmer ◽  
R. W. McCallum
Keyword(s):  
Electrical Stimulation ◽  
Slow Wave ◽  
Test Meal ◽  
Frequency Change ◽  
Fasting State ◽  
Gastric Myoelectrical Activity ◽  
Myoelectrical Activity ◽  
Gastric Slow Wave ◽  
Student’S T ◽  
Student’S T Test

The aims of this study were to 1) investigate gastric myoelectrical activity in patients with gastroparesis, 2) validate the cutaneous electrogastrogram (EGG) in tracking the frequency change of the gastric slow wave, and 3) investigate the effect of electrical stimulation on gastric myoelectrical activity. Gastric myoelectrical activity was recorded in 12 patients with documented gastroparesis using serosal electrodes for > 200 min in each subject. All recordings were made at least 4 days after surgery. Each session consisted of a 30-min recording in the fasting state and a 30-min recording after a test meal. The test meal (liquid or mixed) was selected according to patient's tolerance. Electrical stimulation was performed in three subjects via the serosal electrodes at a frequency of 3 cycles/min. Gastric myoelectrical activity was recorded using serosal electrodes in each session. The serosal recording showed slow waves of 2.5 to 4.0 cycles/min in all 12 subjects. Absence of spikes was noted in 11 of the 12 subjects. The simultaneous serosal and cutaneous recording of gastric myoelectrical activity showed that the frequency of the EGG was exactly the same as that of the serosal recording. Liquid meals resulted in a significant decrease in slow-wave frequency (Student's t test, P = 0.006), and the EGG accurately reflected this change. Electrical stimulation had no effect on the frequency of the gastric slow wave and did not induce spikes.(ABSTRACT TRUNCATED AT 250 WORDS)

The Effect of Changes in Dialysate Volume on Glucose and Urea Equilibration

1994 ◽  
Vol 14 (3) ◽  
pp. 236-239 ◽  
Author(s):  
Edward C. Kohaut ◽  
F. Bryson Waldo ◽  
Mark R. Benfield
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Prospective Study ◽  
Body Surface ◽  
Patient Population ◽  
Different Ages ◽  
Student’S T ◽  
A Prospective Study ◽  
Dialysate Volume ◽  
Student’S T Test

Objectives To determine the effect of changing dialysate volume on urea and glucoseequilibration curves and to determine, if dialysate volume is prescribed on the basis of body surface area, whether equilibration curves will be consistent in patients of different sizes and ages. Design A prospective study wherein children with acute or chronic renal failure had peritoneal equilibration studies done with dwell volumes of 30 mL/kg, 40 mL/kg, and 1200 mL/m2. Patient Population Twenty-two children: 7 under 3 years of age; 8 between 3 and 10 years of age; 7 older than 10 years of age. Statistics Student's t-test. Results Urea and glucose equilibrated rapidly at dwell volumes of 30 mL/kg, slower at dwell volumes of 40 mL/kg, and slowest at dwell volumes of 1200 mL/m2. Equilibration curves were similar in children of different ages when dialysate volumes of 1200 mL/m2 were infused. Conclusion Dialysate volumes of 1200 mL/m2 should be used when equilibration studies are being done to compare individuals of different ages and sizes.

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