scholarly journals STUDI POTENSI LIPASE Alcaligenes faecalis UNTUK APLIKASI BIODETERJEN

2016 ◽  
Vol 3 (2) ◽  
pp. 66
Author(s):  
Ika Rahmatul Layly ◽  
Nita Oktavia Wiguna
Keyword(s):  
Olive Oil ◽  
Culture Medium ◽  
Performance Test ◽  
Economic Value ◽  
Performance Testing ◽  
Alcaligenes Faecalis ◽  
Luria Bertani ◽  
Stability Testing ◽  
Commercial Detergents ◽  
Hydrolysis Of

In detergent industry, enzymes are used enormously in terms of quantity and economic value. Lipase catalyzes the hydrolysis of triglycerides into diglycerides and monoglycerides by releasing fatty acids. Lipase is produced by bacteria, fungi, and yeasts. This study aims to determine the potential of Alcaligenes faecalis lipase  for its application  as biodetergen, through stability testing of its lipase activity against detergent components by exposing the enzyme to the commercial detergents, as well as performance testing through washing. Alcaligenes faecalis lipase was produced using Luria Bertani (LB) culture medium supplemented with 1% olive oil inducer. Production is carried out for 24 hours, and the enzyme was harvested at the 18th hour. The harvested enzyme was tested for their stability after being exposed to commercial detergents at a concentration of 1-5%. Results showed that the exposure to the detergents decreased the enzyme activity to 22, 38, 48, 68 and 90%. Performance test showed that the olive oil impurity removal from the fabric was 29%.Keywords: Alcaligenes faecalis Lipase, biodetergent, lipase activities, washing test AbstrakPada industri detergen penggunaan enzim sangatlah besar baik secara jumlah maupun nilai ekonomi. Lipase mengkatalis hidrolisis trigliserida menjadi digliserida dan monogliserida dengan membebaskan asam lemak. Lipase dihasilkan oleh bakteri, jamur, dan yeast. Penelitian ini bertujuan untuk mengetahui potensi lipase Alcaligenes faecalis untuk aplikasi biodeterjen, melalui uji stabilitas aktivitas lipase terhadap komponen deterjen dengan memaparkan terhadap deterjen komersial serta uji kinerja melalui washing test. Lipase Alcaligenes faecalis diproduksi menggunakan media Luria Bertani (LB) dengan penambahan induser minyak zaitun 1%. Produksi dilakukan selama 24 jam dengan waktu pemanenan enzim pada jam ke-18. Enzim yang sudah dipanen diuji stabilitasnya setelah dipapar dengan deterjen komersial pada konsentrasi 1-5%. Berdasarkan hasil pengujian aktivitas setelah dipapar terlihat penurunan aktivitas berturut-turut sebesar 22, 38, 48, 68 dan 90%. Hasil uji kinerja menunjukkan bahwa noda minyak zaitun yang hilang dari kain sebesar 29%.Kata kunci: Lipase Alcaligenes faecalis, biodeterjen, aktivitas lipase, washing test

scholarly journals Ag/AgCl Reference Electrode Based on Thin Film of Arabic Gum Membrane

2018 ◽  
Vol 18 (3) ◽  
pp. 479 ◽  
Author(s):  
Sagir Alva ◽  
Aiman Sajidah Binti Abdul Aziz ◽  
Mohd Ismahadi Bin Syono ◽  
Wan Adil Bin Wan Jamil
Keyword(s):  
Performance Test ◽  
Reference Electrode ◽  
Performance Testing ◽  
Optimum Concentration ◽  
Stability Testing ◽  
Arabic Gum ◽  
Linear Distance ◽  
Double Junction ◽  
And Performance ◽  
Hydrogel Films

The Ag/AgCl reference electrode based on the Arabic Gum (AG) hydrogel films was successfully developed. The Arabic Gum concentration used in this study was 10–40% (w/v) with an optimum concentration of 20% (w/v). In this study, reference electrode performance testing is based on DmV response of Cl-ion’s measurements, stability tests and performance testing against ISE sensors such as K+, NH4+, and NO3-. The Ag/AgCl based reference electrode of the Arabic Gum film shows an average DmV of 4.0 ± 0.7 mV when response test is performed against a standard Ag/AgCl double junction electrode in various concentrations of Cl-ion’s. Meanwhile, in stability testing conducted in a 0.01 M KCl solution for 72 h found drift of < 0.6 mV/h. The reference performance test of the Arabic Gum reference electrode with three types of ISE NH4+, K+ and NO3- commercial sensors obtaining a slope closer to the Nernst value is 54.9 ± 0.9, 52.3 ± 0.5 and -53.2 ± 0.2 mV/dec with all having a linear distance of 0.1–10-5 M.

Evaluation of Asphalt Mixture Performance Using Cracking and Durability Tests at a Full-Scale Pavement Facility

2021 ◽  
pp. 036119812110218
Author(s):  
Amir Golalipour ◽  
Varun Veginati ◽  
David J. Mensching
Keyword(s):  
Performance Test ◽  
Asphalt Mixture ◽  
Performance Testing ◽  
Field Performance ◽  
Optimization Procedure ◽  
Cyclic Fatigue ◽  
Intermediate Temperature ◽  
Index Test ◽  
Reclaimed Asphalt ◽  
Indirect Tensile

In the asphalt materials community, the most critical research need is centered around a paradigm shift in mixture design from the volumetric process of the previous 20-plus years to an optimization procedure based on laboratory-measured mechanical properties that should lead to an increase in long-term pavement performance. This study is focused on advancing the state of understanding with respect to the value of intermediate temperature cracking tests, which may be included in a balanced mix design. The materials included are plant-mixed, laboratory-compacted specimens reheated from the 2013 Federal Highway Administration’s (FHWA’s) Accelerated Loading Facility (ALF) study on reclaimed asphalt pavement/reclaimed asphalt shingle (RAP/RAS) materials. Six commonly discussed intermediate temperature (cracking and durability) performance testing (i.e., Asphalt Mixture Performance Tester [AMPT] Cyclic Fatigue, Cantabro, Illinois Flexibility Index Test [I-FIT], Indirect Tensile Cracking [ITC, also known as IDEAL-CT], Indirect Tensile Nflex, and Texas Overlay Test) were selected for use in this study based on input from stakeholders. Test results were analyzed to compare differences between the cracking tests. In addition, statistical analyses were conducted to assess the separation among materials (lanes) for each performance test. Cyclic fatigue and IDEAL-CT tests showed the most promising results. The ranking from these two tests’ index parameters matched closely with ALF field performance. Furthermore, both showed reasonable variability of test data and they were successful in differentiating between different materials.

Performance Testing of Coal Fired Power Plants

2007 ◽  
Author(s):  
Shane E. Powers ◽  
William C. Wood
Keyword(s):  
Power Plant ◽  
Power Plants ◽  
Performance Test ◽  
Model Development ◽  
Performance Testing ◽  
The United States ◽  
Plant Performance ◽  
Cycle Performance ◽  
Test Program

With the renewed interest in the construction of coal-fired power plants in the United States, there has also been an increased interest in the methodology used to calculate/determine the overall performance of a coal fired power plant. This methodology is detailed in the ASME PTC 46 (1996) Code, which provides an excellent framework for determining the power output and heat rate of coal fired power plants. Unfortunately, the power industry has been slow to adopt this methodology, in part because of the lack of some details in the Code regarding the planning needed to design a performance test program for the determination of coal fired power plant performance. This paper will expand on the ASME PTC 46 (1996) Code by discussing key concepts that need to be addressed when planning an overall plant performance test of a coal fired power plant. The most difficult aspect of calculating coal fired power plant performance is integrating the calculation of boiler performance with the calculation of turbine cycle performance and other balance of plant aspects. If proper planning of the performance test is not performed, the integration of boiler and turbine data will result in a test result that does not accurately reflect the true performance of the overall plant. This planning must start very early in the development of the test program, and be implemented in all stages of the test program design. This paper will address the necessary planning of the test program, including: • Determination of Actual Plant Performance. • Selection of a Test Goal. • Development of the Basic Correction Algorithm. • Designing a Plant Model. • Development of Correction Curves. • Operation of the Power Plant during the Test. All nomenclature in this paper utilizes the ASME PTC 46 definitions for the calculation and correction of plant performance.

A Comparative Study of Genetic Algorithms and Gradient Methods for RM12 Turbofan Engine Diagnostics and Performance Estimation

2004 ◽  
Author(s):  
Tomas Gro¨nstedt ◽  
Markus Wallin
Keyword(s):  
Gas Turbine ◽  
Performance Test ◽  
Gradient Methods ◽  
Performance Testing ◽  
Performance Estimation ◽  
Data Sets ◽  
Data Set ◽  
Turbofan Engine ◽  
Local Optima ◽  
Bypass Ratio

Recent work on gas turbine diagnostics based on optimisation techniques advocates two different approaches: 1) Stochastic optimisation, including Genetic Algorithm techniques, for its robustness when optimising objective functions with many local optima and 2) Gradient based methods mainly for their computational efficiency. For smooth and single optimum functions, gradient methods are known to provide superior numerical performance. This paper addresses the key issue for method selection, i.e. whether multiple local optima may occur when the optimisation approach is applied to real engine testing. Two performance test data sets for the RM12 low bypass ratio turbofan engine, powering the Swedish Fighter Gripen, have been analysed. One set of data was recorded during performance testing of a highly degraded engine. This engine has been subjected to Accelerated Mission Testing (AMT) cycles corresponding to more than 4000 hours of run time. The other data set was recorded for a development engine with less than 200 hours of operation. The search for multiple optima was performed starting from more than 100 extreme points. Not a single case of multi-modality was encountered, i.e. one unique solution for each of the two data sets was consistently obtained. The RM12 engine cycle is typical for a modern fighter engine, implying that the obtained results can be transferred to, at least, most low bypass ratio turbofan engines. The paper goes on to describe the numerical difficulties that had to be resolved to obtain efficient and robust performance by the gradient solvers. Ill conditioning and noise may, as illustrated on a model problem, introduce local optima without a correspondence in the gas turbine physics. Numerical methods exploiting the special problem structure represented by a non-linear least squares formulation is given special attention. Finally, a mixed norm allowing for both robustness and numerical efficiency is suggested.

scholarly journals Production, characterization and application of inulinase from fungal endophyte CCMB 328

2012 ◽  
Vol 84 (2) ◽  
pp. 443-454 ◽  
Author(s):  
Diego S. Nascimento ◽  
Gildomar Valasques Junior ◽  
Pedro Fernandes ◽  
Geise C.A. Ribeiro ◽  
Danyo M. Lima ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Yeast Extract ◽  
Arid Region ◽  
Culture Medium ◽  
Fungal Endophyte ◽  
Initial Concentration ◽  
Inulin Hydrolysis ◽  
Semi Arid Region ◽  
Hydrolysis Of ◽  
Semi Arid

Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).

scholarly journals Performance Test of a Household Rocket Stoves Fired with Coconut Frond, Coconut Shell and Bamboo

2021 ◽  
Vol 8 (1) ◽  
pp. 59-64
Author(s):  
Almuzakkir . ◽  
Muhammad . ◽  
Adi Setiawan
Keyword(s):  
Thermal Efficiency ◽  
Fossil Fuels ◽  
Performance Test ◽  
Performance Testing ◽  
Biomass Energy ◽  
Test Method ◽  
Fuel Oil ◽  
Coconut Shell ◽  
Water Boiling Test ◽  
A Value

Fuel is something that is very important in everyday life. Almost every human being needs fuel to meet their needs and support their activities, for example cooking in household needs. Currently, fossil fuels or fuel oil (BBM) are still widely used to meet demand, however, it should be noted that fossil fuels or fuel oil (BBM) are non-renewable natural resources. The biomass rocket stove is one of the modern stove innovations that uses biomass energy as the main energy source. Rocket stoves are designed to increase fuel efficiency with thermal efficiency, a combination of the increased combustion efficiency and heat transfer associated with burning briquette fuel. The purpose of this research is to design and manufacture rocket stove fired with coconut and bamboo biomass for household needs as well as developing methods and equipment for performance testing of rocket stoves. In this study, several steps were carried out, including designing a rocket furnace, selecting biomass fuel and testing the performance of a rocket furnace. From the design of the biomass stove, it is noteworthy that the design with two holes makes the combustion air easily enters and makes combustion in the furnace more perfect and efficient. Water boiling test using three types of solid fuels with the cold start condition suggested that the highest thermal efficiency was coconut fronds with a value of 38% and the lowest thermal efficiency was found from coconut shell combustion, i.e. 22%. During hot start test, the highest thermal efficiency was obtained from coconut fronds firing with a value of 41%. Moreover, with simmer water boiling test method, firing the rocket stove with coconut fronds showed the highest thermal efficiency with a value of 37%. Keywords: Rocket Stoves, Coconut Fronds and Shells, Bamboo, Thermal Efficiency, .Water Boiling Tests.

scholarly journals Phenotypic Variation of Tobramycin and Ofloxacin Resistance of Pseudomonas aeruginosa by Repeated Exhibition

2020 ◽  
pp. 1-9
Author(s):  
Andry Maharo Andrianarivelo ◽  
Christian Emmanuel Mahavy ◽  
Blandine Andrianarisoa ◽  
Tsiry Rasamiravaka
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenotypic Variation ◽  
Culture Medium ◽  
Wild Strain ◽  
Resistance Mechanisms ◽  
Luria Bertani ◽  
University Hospital ◽  
Buffer System ◽  
Visual Evaluation ◽  
Almost All

Pseudomonas aeruginosa has the ability to resist almost all available antibiotics by rapidly accumulating multiple resistance mechanisms and thus lead to a therapeutic impasse and higher mortality in infected patients. The objective of this study was to assess the phenotypic variation in resistance to tobramycin and ofloxacin from Pseudomonas aeruginosa by repeated exhibition after determination of the minimum inhibitory concentration. This is a prospective and descriptive study carried out in the Laboratory of Microbiology of Fundamental and Applied Biochemistry (Faculty of Sciences, Antananarivo) during the month of January 2020. The strains studied were the virulent wild strain of Pseudomonas aeruginosa PAO1 supplied by the Laboratory and two clinical strains of Pseudomonas aeruginosa from the Microbiology Laboratory of the Joseph Ravoahangy Andrianavalona University Hospital Center, Antananarivo. The strains of P. aeruginosa were cultured in the liquid culture medium (which is Luria Bertani, added with a buffer system of 3- (N-morpholino) propanesulfonic acid (LB-MOPS) which will stabilize the pH and a solid culture medium which is Columbia agar. Repeated exhibition to Tobramycin and Ofloxacin from these strains have been made. The MIC is determined by a visual evaluation of the turbidity of the various wells of the microplate. The MIC value of Pseudomonas aeruginosa with tobramycin and ofloxacin is very variable for the initial MIC until the 5th generation after repeated exhibition. More Pseudomonas aeruginosa is exposed to an antibiotic many times, the more it develops resistance to this antibiotic, even being sensitive at the start. That is to say, clinically, the dose prescribed for the antibiotic has been greatly exceeded if Pseudomonas aeruginosa is repeatedly exposed to the same antibiotic.

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