scholarly journals Genotyping of “Ukrainian” water buffaloes according β-CN (A2-milk), CSN3 and βLG genes

2021 ◽  
Vol 59 (3) ◽  
pp. 361-365
Author(s):  
N. B. Mokhnachova
Keyword(s):  
Restriction Enzyme ◽  
Molecular Genetic ◽  
Animal Husbandry ◽  
Bubalus Bubalis ◽  
Molecular Genetic Analysis ◽  
Meat Production ◽  
Casein Gene ◽  
Water Buffaloes ◽  
Beta Casein ◽  
Beta Lactoglobulin

Buffalo breeding in Ukraine is an ancient traditional branch of animal husbandry of the Crimean Tatars and Rusyns of Transcarpathia. Basically, "Ukrainian" buffaloes belong to the river buffalo (Bubalus bubalis) and are bred for dairy and meat production. Polymorphism of genes associated with dairy productivity will allow breeding buffaloes taking into account the “desired” genotypes in relation to economically useful traits. The paper dwells on studying allelic polymorphisms of beta-casein (β-CN), kappa-casein (CSN3) and beta-lactoglobulin (β-LG) genes in population of water buffaloes bred in Ukraine using PCR followed by restriction hydrolysis of the formed fragments (PCR-RFLP). Results of study of the “Ukrainian” population of water buffaloes are discussed, namely: the frequency of genotypes and alleles at the loci of beta-casein, kappa-casein and beta-lactoglobulin genes. Amplified fragment β-CN with the length of 121 bp was digested with DdeI restriction enzyme. A feature of the allelic spectrum of the beta-casein gene (β-CN) in the studied population was absence of A1 allele. All animals carried the β-CNA2A2 genotype of beta-casein gene, respectively, β-CNA2 allele frequency was 1.0. For the CSN3 gene, an amplified fragment in 273 bp was digested with HinfI restriction enzyme. A 100% predominance of animals with the most preferred homozygous CSN3BB genotype was revealed. During the β-LG gene study process, an amplified fragment with a size of 247 bp was digested with HaeIII. It has been determined that the most frequent was allele βLGA and genotype βLGAA of beta-lactoglobulin gene (0.96 and 0.92, respectively). Heterozygous β-LGAB genotype is present in 8 % of buffaloes. The research results are of interest in the field of molecular genetic analysis of the buffalo genome, which are the source of specific properties. The data obtained can be useful for preserving and increasing the genetic diversity of the “Ukrainian” population of water buffaloes, as well as for obtaining valuable products from buffaloes.

scholarly journals The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis

2020 ◽  
Vol 24 (7) ◽  
pp. 747-754
Author(s):  
V. R. Kharzinova ◽  
N. A. Zinovieva
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Animal Husbandry ◽  
Molecular Genetic Analysis ◽  
Fixation Index ◽  
Large White ◽  
Black And White ◽  
The Republic

One of the main tasks of genetics and animal breeding is the assessment of genetic diversity and the study of genetic relationships between different breeds and populations using molecular genetic analysis methods. We analysed the polymorphism of microsatellites and the information on the state of genetic diversity and the population structure of local breeds in Russia: the Kemerovo, the Berkshire, the Liven, the Mangalitsa, and the Civilian; in the Republic of Belarus: the Large White and the Black-and-White; and in Ukraine: the White Steppe, as well as commercial breeds of imported origin of domestic reproduction: the Large White, the Landrace, and the Duroc. The materials used for this study were the tissue and DNA samples extracted from 1,194 pigs and DNA of the UNU “Genetic material bank of domestic and wild animal species and birds” of the L.K. Ernst Federal Research Center for Animal Husbandry. Polymorphisms of 10 microsatellites (S0155, S0355, S0386, SW24, SO005, SW72, SW951, S0101, SW240, and SW857) were determined according to the previously developed technique using DNA analyser ABI3130xl. To estimate the allele pool of each population, the average number of alleles (NA), the effective number of alleles (NE ) based on the locus, the rarified allelic richness (AR), the observed (HO ) and expected (HE ) heterozygosity, and the fixation index (FIS) were calculated. The degree of genetic differentiation of the breeds was assessed based on the pairwise values of FST and D. The analysis of the allelic and genetic diversity parameters of the local breeds showed that the maximum and minimum levels of polymorphism were observed in pigs of the Ukrainian White Steppe breed (NA = 6.500, NE = 3.709, and AR = 6.020) and in pigs of the Duroc breed (NA = 4.875, NE = 2.119, and AR = 3.821), respectively. The highest level of genetic diversity was found in the Large White breed of the Republic of Belarus (HO = 0.707 and NE = 0.702). The minimum level of genetic diversity was found in pigs of the imported breeds – the Landrace (HO = 0.459, HE = 0.400) and the Duroc (HO = 0.480, HE = 0.469) – indicating a high selection pressure in these breeds. Based on the results of phylogenetic analysis, the genetic origin of Large White pigs, the breeds, from which the Berkshire pigs originated, and the genetic detachment of the Landrace from the Mangalitsa breeds were revealed. The cluster analysis showed a genetic consolidation of the Black-and-White, the Berkshire, and the Mangalitsa pigs. Additionally, the imported breeds with clustering depending on the origin were characterised by a genetic structure different from that of the other breeds. The information obtained from these studies can serve as a guide for the management and breeding strategies of the pig breeds studied, to allow their better use and conservation.

Water buffaloes (Bubalus bubalis) only have A2A2 genotype for beta-casein

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Louise Sarmento Martins de Oliveira ◽  
Jackeline Santos Alves ◽  
Marisa Siva Bastos ◽  
Valdecy Aparecida Rocha da Cruz ◽  
Luís Fernando Batista Pinto ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
Water Buffaloes ◽  
Beta Casein

scholarly journals The Effect of Myostatin Gene Polymorphisms onSome Biochemist Traits for Blood in Broiler Chicken

2018 ◽  
Vol 7 (1) ◽  
pp. 77-86
Author(s):  
Ismail Habeeb Ismail ◽  
Adnan Hussein Mohammed
Keyword(s):  
Restriction Enzyme ◽  
Broiler Chicken ◽  
Molecular Genetic ◽  
Restriction Enzymes ◽  
Molecular Genetic Analysis ◽  
College Of Agriculture ◽  
Biochemical Traits ◽  
Myostatin Gene ◽  
Enzyme Form ◽  
Blood Biochemical

This study is conducted at the Poultry Farm of Animal Production Department of College of Agriculture, University of Al-Qadisiyah during the period 9/4/2015 - 20/5/2015 and in a laboratory of molecular genetic analysis of the College of Agriculture, University of Baghdad and Almusayab Bridge Company. The objective of this study is to identify the genotypes for Myostatin gene (GDF-8), and its relationship with physiological traits of broiler chicken. Three hundreds of Ross 308 chicks at day-old were used. The experiment continues until the sixth week of age. Three types of restriction enzymes (Aci I, Bbv I and Bbs I) are used The results of this study could be summarized as follows. The polymorphisms of the Myostatin gene achieves using Restriction enzyme (Aci) are GG, GA and AA respectively. The genotype of  Myostatin gene  has no  effect on   biochemical traits  during 21  and  42 day, except which the effect of the genotypes of the Myostatin gene on the serum albumin is highly significant  (P<0.01) during 21 day. And also the form genotype of the Myostatin gene with Restriction enzyme form (Bbs I) are CC, CT and TT respectively. The genotype of Myostatin gene has no effect on the blood biochemical during 21 and 42 day. The results show that the genotype of the Myostatin gene with Restriction enzyme form (Bbv I) are AA and GA respectively. The effect of has no effect on the blood biochemical during 21 and 42 day.

124 KNOCKING-IN OF A FOREIGN GENE ON A BOVINE BETA-CASEIN GENE INTO BOVINE PRIMARY FIBROBLASTS USING HOMOLOGOUS RECOMBINATION EVENTS

2006 ◽  
Vol 18 (2) ◽  
pp. 171
Author(s):  
M. Chang ◽  
K.-B. Oh ◽  
J.-S. Park ◽  
D.-B. Koo ◽  
S.-T. Shin ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Blastocyst Stage ◽  
Genomic Region ◽  
Selection Marker ◽  
Foreign Gene ◽  
Casein Gene ◽  
Primary Fibroblasts ◽  
Beta Casein ◽  
Unique Restriction ◽  
Targeting Vector

Knocking-in of a foreign gene on a tissue-specific genomic region has not been reported in livestock. In this study, we constructed two knock-in vector cassettes specific for the bovine beta-casein gene which is expressed only in the mammary gland during lactation. The targeting vector cassettes, pBCKI1 and pBCKI2, have homology regions for the bovine beta-casein gene and contain 13.1 kb and 9.1 kb targeting arms with different long arm lengths, respectively. The targeting vector cassettes have unique restriction enzyme sites for insertion of foreign therapeutic genes in front of the neo gene which was inserted into the vector as a selection marker. The human thrombopoietin (hTPO) gene was inserted into the restriction enzyme sites of both targeting vectors, which were named pBCTPOKI1 and pBCTPOKI2. When the two targeting vectors were transfected into bovine ear skin fibroblasts using Lipofectamine (Invitrogen, Seoul, South Korea), 6.3% of neo resistant clones (2/32) were homologously targeted with the pBCTPOKI2 vector. Cells from the targeted colonies were nuclear-transferred into enucleated bovine oocytes and cultured to the blastocyst stage. A total of 66 blastocysts were generated of which 46 were transferred into recipients. No offspring have been produced at this time. Here we first describe knock-in vectors specific for a bovine beta-casein gene, which will be employed to generate animal bioreactors that produce therapeutic proteins secreted into the milk.

scholarly journals 31 CONSTRUCTION OF A TARGETING VECTOR SPECIFIC FOR THE BOVINE BETA-CASEIN GENE

2005 ◽  
Vol 17 (2) ◽  
pp. 165
Author(s):  
M. Chang ◽  
K.-B. Oh ◽  
G. Wee ◽  
D.-B.n Koo ◽  
S.-T.e Shin ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Gene Targeting ◽  
Restriction Enzyme ◽  
Transfection Efficiency ◽  
Therapeutic Proteins ◽  
Selection Marker ◽  
Specific Gene ◽  
Casein Gene ◽  
Beta Casein ◽  
Targeting Vector

Although using livestock as animal bioreactors is a powerful tool to produce valuable therapeutic proteins in the milk, there are still some limitations to the technology such as a low frequency of transgenesis and a low expression of the transgenes by random integration. In this study, we constructed gene-targeting vectors for a mammary gland-specific gene and then obtained two homologous recombinant cell clones after transfection of the vectors into bovine somatic cells. Two targeting vector cassettes, BCKI I and BCKI II, which have homology regions for a bovine beta-casein gene, were constructed. The beta-casein gene is expressed only in the mammary gland and is the most abundant milk protein in the cow. The targeting sequence lengths of the BCKI I and BCKI II vector cassettes were 13.1 kb and 9.1 kb, respectively, and contained different long arm lengths. A neo gene was inserted into the vectors as a selection marker, and a few restriction enzyme sites were made in front of the neo gene. The human thrombopoietin (TPO) gene was inserted into the restriction enzyme sites of the vector cassettes, named BCTPOKI I and BCTPOKI II vectors. The BCTPOKI I and BCTPOKI II vectors were transfected into bovine embryonic fibroblasts (bEF) and ear skin fibroblasts (bESF) using Lipofectamine™ 2000 reagent (Invitrogen, Seoul, South Korea). In order to determine the highest transfection efficiency, a variety of factors such as DNA concentration, lipid volume, and exposure time to DNA-liposome complexes based on the manufacturers' guideline, was optimized. The 2:1 and 1:2 ratios of DNA (μg) to transfection reagent (μL) were efficient for bEF and bESF, respectively, under overnight exposure to DNA-Lipofectamine™ 2000 reagent. Seventeen percent (51/304) of bESF clones and 6% (9/149) of bEF clones were normally expanded into passage 8. PCR and Southern blotting indicated that 6.3% (2/32) of the clones carrying with BCTPOKI II vectors was homologously targeted at the beta-casein gene. However, none (0/60) of the clones carrying BCTPOKI I was targeted. Additionally, both of the targeted clones were from bESF. When the targeted cells were transferred into enucleated oocytes and cultured, 83% (43/52) of the cloned embryos were transgenic. Thus, we found that homologous recombinant events using gene-targeting vectors might be dependent on cell types, vector sizes, and transfection procedures. In conclusion, mammary gland-specific gene-targeting vectors coupled with somatic cell nuclear transfer technology will be very useful for developing animal bioreactors that produce therapeutic proteins in milk.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

Diagnosis and Management of Cardiomyopathies – A Focus on Genetics, Cardiac Magnetic Resonance and Clinical Features

2011 ◽  
Vol 7 (3) ◽  
pp. 225
Author(s):  
Gianfranco Sinagra ◽  
Michele Moretti ◽  
Giancarlo Vitrella ◽  
Marco Merlo ◽  
Rossana Bussani ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Imaging Techniques ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Routine Clinical Practice ◽  
Clinical Approach ◽  
Diagnosis And Management ◽  
Made In

In recent years, outstanding progress has been made in the diagnosis and treatment of cardiomyopathies. Genetics is emerging as a primary point in the diagnosis and management of these diseases. However, molecular genetic analyses are not yet included in routine clinical practice, mainly because of their elevated costs and execution time. A patient-based and patient-oriented clinical approach, coupled with new imaging techniques such as cardiac magnetic resonance, can be of great help in selecting patients for molecular genetic analysis and is crucial for a better characterisation of these diseases. This article will specifically address clinical, magnetic resonance and genetic aspects of the diagnosis and management of cardiomyopathies.

SELECTION OF CATTLE FOR POLYMORPHIC BETA-CASEIN GENE IN THE KRASNODAR TERRITORY

2019 ◽  
pp. 22-26 ◽  
Author(s):  
N. KOVALYUK ◽  
◽  
V. SATSUK ◽  
M. KOVALYUK ◽  
E. MACHULSKAYA ◽  
...  
Keyword(s):  
Casein Gene ◽  
Beta Casein ◽  
Selection Of
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