scholarly journals Kinetic Spectrophotometric Method for the Estimation of Ofloxacin in Pharmaceutical Formulations

2017 ◽  
Vol 56 (4) ◽  
Author(s):  
Lalit Kishore ◽  
Ashok Kumar ◽  
Anroop Nair ◽  
Navpreet Kaur
Keyword(s):  
Spectrophotometric Method ◽  
Reference Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Of Change ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Significant Difference ◽  
Rate Method ◽  
Kinetic Spectrophotometric

The objective of the current study was to develop a direct, sensitive spectrophotometric method based on the oxidation of Ofloxacin using potassium permanganate in alkaline medium. The rate of change of absorbance was measured at 603 nm. The initial rate method and fixed time method (at 4 min) are utilised to construct calibration graphs for calculating the concentration of the drug. The results were validated through inter day and intraday precision assays according to the ICH guidelines and also through recovery studies. Statistical comparison of the proposed methods with that of reference method shows excellent agreement and indicates no significant difference in their accuracy and precision.

scholarly journals Kinetic Spectrophotometric Determination of Certain Cephalosporins in Pharmaceutical Formulations

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Osama H. Abdelmageed ◽  
Tamer Z. Attia
Keyword(s):  
Initial Rate ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Of Change ◽  
Ceftriaxone Sodium ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Cephalosporin Antibiotics ◽  
Kinetic Spectrophotometric

A simple, reliable, and sensitive kinetic spectrophotometric method was developed for determination of eight cephalosporin antibiotics, namely, Cefotaxime sodium, Cephapirin sodium, Cephradine dihydrate, Cephalexin monohydrate, Ceftazidime pentahydrate, Cefazoline sodium, Ceftriaxone sodium, and Cefuroxime sodium. The method depends on oxidation of each of studied drugs with alkaline potassium permanganate. The reaction is followed spectrophotometrically by measuring the rate of change of absorbance at 610 nm. The initial rate and fixed time (at 3 minutes) methods are utilized for construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 5–15 g  and 5–25 g  using the initial rate and fixed time methods, respectively. The results are validated statistically and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations

2015 ◽  
Vol 50 ◽  
pp. 169-178
Author(s):  
Mouhammed Khateeb ◽  
Basheer Elias ◽  
Fatema Al Rahal
Keyword(s):  
Folic Acid ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Sulfuric Acid Medium ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A simple and sensitive kinetic spectrophotometric method has been developed for the determination of folic acid (FA) in bulk and pharmaceutical Formulations. The method is based on the oxidation of FA by Fe (III) in sulfuric acid medium. Fe (III) subsequently reduces to Fe (II) which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance at λmax 725 nm. The rate data and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1–20 μg mL-1 for each method. The correlation coefficient was 0.9978 and 0.9993, and LOD was found to be 0.91 and 0.09 μg mL-1 for rate data and fixed time methods, respectively. The proposed method has been successfully applied to the determination of FA in formulations with no interference from the excipients. Statical comparison of the results shows that there is no significant difference between the proposed and pharmacopoeial methods

scholarly journals A kinetic spectrophotometric method for the determination of lansoprazole in pharmaceutical formulations

2006 ◽  
Vol 71 (10) ◽  
pp. 1107-1120 ◽  
Author(s):  
Nafisur Rahman ◽  
Zehra Bano ◽  
Hejaz Azmi ◽  
Mohammad Kashif
Keyword(s):  
Spectrophotometric Method ◽  
Room Temperature ◽  
Pharmaceutical Formulations ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results ◽  
Calibration Equations ◽  
Method Show

Asimple kinetic spectrophotometric method has been developed for the determination of lansoprazole in pharmaceutical formulations. The method is based on the oxidation of the drug with alkaline potassium permanganate at room temperature. The reaction was followed spectrophotometrically by measuring the increase in the absorbance owing to the formation of MnO 42? at 610 nm (Method A) and the decrease in the absorbance at 530 nm due to the disapperance of MnO4? (Method B). Calibration procedures were adopted for the assay of the drug. The calibration curves were linear over the concentration ranges of 5-150 and 5-70?g ml-1, with the corresponding calibration Equations: rate = -3.915x10-6 + 5.271x10-5 c and ?A = 1.04x 10-3 + 1.78x10-3 c for methods A, and B, respectively. A statistical comparison of the results of the proposed procedures with those of the reference spectrophotometric method show excellent agreement and indicated no significant difference between the compared methods in terms of accuracy and precision. Interval hypothesis tests were also performed, which indicated that the true bias of all samples was less than ? 2 %. .

scholarly journals New Kinetic Spectrophotometric Method for Determination of Fexofenadine Hydrochloride in Pharmaceutical Formulations

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Safwan Ashour ◽  
Mouhammed Khateeb
Keyword(s):  
Spectrophotometric Method ◽  
Initial Rate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Official Method ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method was developed for the determination of fexofenadine hydrochloride in bulk and pharmaceutical preparations. The method is based on a kinetic investigation of the oxidation reaction of fexofenadine using alkaline potassium permanganate as an oxidizing agent at room temperature. The reaction is followed spectrophotometrically by measuring the increase of absorbance owing to the formation of manganate ion at 610 nm. The initial rate and fixed time (at 15 min) methods are utilized for construction of calibration graphs. All the reaction conditions for the proposed method have been studied. The linearity range was found to be 2.5–50.0 μg mL−1 with detection limit of 0.055 μg mL−1 for both initial rate and fixed time methods. The proposed method was applied successfully for the determination of fexofenadine in pharmaceutical formulations; the percentage recoveries were 99.98–101.96%. The results obtained were compared statistically with those obtained by the official method and showed no significant differences regarding accuracy and precision.

scholarly journals A Sensitive UV Spectrophotometric Method for the Determination of Gabapentin

2009 ◽  
Vol 6 (s1) ◽  
pp. S163-S170 ◽  
Author(s):  
R. Singh Gujral ◽  
S. Manirul Haque ◽  
P. Shanker
Keyword(s):  
Spectrophotometric Method ◽  
Good Accuracy ◽  
Limit Of Detection ◽  
Low Cost ◽  
Pharmaceutical Formulations ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Limit Of Quantitation ◽  
Precision Limit

An accurate and validated spectrophotometric method was developed for the determination of gabapentin. This is simple, sensitive and low cost UV spectrophotometric method. The method is based on the direct measurement of the native absorbance of the drug. The detection was done at 210 nm. The method was linear in the range of 0.25 - 3.5 µ g/mL with correlation coefficient of 0.9999. It is validated according to the ICH guidelines with respect to linearity, selectivity, accuracy and precision, limit of quantitation and limit of detection. The method has been applied to assess gabapentin in pharmaceutical formulations with good accuracy and precision and relatively free of interference from coexisting substances.

scholarly journals Development and application of spectrophotometric method for quantitative determination of Metronidazole in pure and tablet formulations

2016 ◽  
Vol 2 (1) ◽  
pp. 28 ◽  
Author(s):  
Zeeshan Masood ◽  
Muhammad Tayyab Ansari ◽  
Sharjeel Adnan ◽  
Muhammad Asad ◽  
Muhammad Farooq ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Nitrite Ion ◽  
Colored Complex ◽  
Accuracy And Precision ◽  
Significant Difference

A rapid, simple and sensitive spectrophotometric method has been developed for the determination of metronidazole in pharmaceutical pure and dosage forms. The method depends on alkaline hydrolysis of metronidazole releases the nitro group as nitrite ion and yielded nitrite ions can be used to give a colored complex that absorbs maximally at 505 nm. Beer’s law is obeyed in the concentration ranges 9-100 mg/ml with molar absorptivity of 1.14 ×103 L mole-1 cm-1. The proposed method is precise, accurate and specific for the quantitative determination of drug in bulk and dosage forms. The results of analysis of commercial formulations and the recovery study of metronidazole suggested that there is no interference from any excipients, which are present in pharmaceutical formulations of metronidazole. Statistical comparison of the results was performed with regard to accuracy and precision using student’s t-test and F-ratio at 95% confidence level. There is no significant difference between the reported and proposed methods with regard to accuracy and precision.

scholarly journals A Novel Use of 3-Methyl-2-Benzothiazolinone Hydrazone Hydrochloride Monohydrate for Kinetic Spectrophotometric Determination of Captopril in Pharmaceutical Formulations

2016 ◽  
Vol 71 ◽  
pp. 29-39
Author(s):  
Mouhammed Khateeb ◽  
Bashir Elias ◽  
Shahama Adi
Keyword(s):  
Correlation Coefficients ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Significant Difference ◽  
Colored Product ◽  
Hydrochloride Monohydrate ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A new, simple and sensitive kinetic spectrophotometric method has been proposed for the determination of captopril (CPT) in pharmaceutical formulations. The method is based on oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) by ferric chloride followed by its coupling with the drug to form green-yellow colored product with absorbance maximum at 395nm. The concentration of CPT was calculated using the calibration equation for the rate data and fixed time methods. The linearity range was found to be 0.5–22.5 μg mL-1for each method. The correlation coefficients were 0.9994 and 0.9971 for rate data and fixed time methods respectively. The proposed methods were applied successfully for the determination of CPT in pharmaceutical formulations. Statistical comparison of the results shows that there is no significant difference between the proposed and official methods.

scholarly journals NEW ANALYTICAL METHODS FOR ESTIMATION OF ARTEETHER BY UV AND FLUORESCENCE SPECTROPHOTOMETRY: DEVELOPMENT AND VALIDATION

2018 ◽  
Vol 8 (5) ◽  
pp. 151-158
Author(s):  
Shikha Thakur ◽  
Neha Bajwa ◽  
Ashish Baldi
Keyword(s):  
Uv Spectroscopy ◽  
Research Work ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Dosage Form ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Significant Difference ◽  
Development And Validation ◽  
First Time

The present research work discusses the development and validation of two different spectrophotometric methods for estimation of α-β arteether using UV spectrophotometer and spectrofluorimeter for the first time. Two simple, accurate, precise, sensitiveand economical methods has been developed and validated for the estimation of α-β arteether in bulk and pharmaceutical dosage form as per ICH guidelines Q2(R1). The solvent used for UV spectroscopy was methanol and HCl (8:2) and methanol was used for fluorimeter. For qualitative and quantitative analysis, 254 nm was used in UV spectroscopy and excitation and emission wavelengths were set at 354 nm and 697 nm respectively for fluorimetry. Coefficients of correlation were found to be 0.993 and 0.992 for UV spectroscopy and fluorimetry. Both methods show good accuracy and precision and were compared statistically by using two way ANOVA which shows no significant difference between these methods. So the proposed methods were found to have equal applicability for estimation and routine analysis of arteether in pharmaceutical formulations. Keywords: Arteether, Analytical method, Fluorimeter, Pharmaceutical formulation, UV spectrophotometer.

Development of Validated UV Spectrophotometric Method for Assay of Ozagrel and its Pharmaceutical Formulations

2018 ◽  
pp. 69-77 ◽  
Author(s):  
Vishal N Kushare ◽  
Sachin S Kushare ◽  
Sagar V Ghotekar
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Routine Practice ◽  
Quantitation Limit ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Standard Values ◽  
Bulk Drug

UV Spectrophotometric method was developed and validated for the quantitative determination of Ozagrel in bulk drug and in pharmaceutical formulations. Ozagrel shows the maximum absorbance at 270 nm. Ozagrel follows Beer’s law in the concentration range of 1.0-10.0 µg/ml (r = 0.999). The detection limit (DL) and quantitation limit (QL) were 0.4629 and 1.4027 µg/ml respectively. Accuracy and precision were found to be satisfactory. The developed methods were validated according to ICH guidelines. All the validation parameters were found to be satisfactory accordance with the standard values. Therefore, the proposed method can be used for routine practice for the determination of Ozagrel in assay of bulk drug and pharmaceutical formulations.

New Derivatization Methodology of 4-aminobenzoic Acid from its Dietary Supplements: Kinetic Spectrophotometric Methods for Determination

2019 ◽  
Vol 15 (7) ◽  
pp. 752-768
Author(s):  
Naser A. Naser ◽  
Kasim M. Alasedi ◽  
Zainab A. Khan
Keyword(s):  
Dietary Supplement ◽  
Initial Rate ◽  
Kinetic Method ◽  
Reference Method ◽  
Fixed Time ◽  
Proton Nuclear Magnetic Resonance ◽  
Aminobenzoic Acid ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Kinetic Spectrophotometric

Background: A new approach describing the validation and development of an easy, new spectrophotometric and kinetic method for identification of para-aminobenzoic acid in dietary supplement has been performed. In this study, para-aminobenzoic acid was derived in a pH-controlled environment, as a new organic compound 4(4-Benzophenylazo)pyrogallol, by incorporating diazotized para-aminobenzoic acid with pyrogallol. Objective: The determination of para-aminobenzoic acid was conducted by the fixed time and initial rate techniques. These approaches were based on the reaction of the compound containing paraaminobenzoic acid, 4(4-Benzophenylazo)pyrogallol, with Ag(I) to form colored product with a maximum absorbance at 468nm. Both of these techniques were adopted for constructing the calibration curves and examined for their suitability for the quantitation of para-aminobenzoic acid in dietary supplement. Methods: The determination process was established, using initial rate and fixed time kinetic spectrophotometric methods. Results: 4(4-Benzophenylazo)pyrogallol was characterized using proton-nuclear magnetic resonance, Fourier-transform infrared, differential scanning calorimetry and thermogravimetric thermal methods, gas chromatography–mass techniques, and solvatochromic behavior in solvents with different polarities was also examined. Conclusion: For the first time, para-aminobenzoic acid was well determined by incorporating it as an organic solid compound, 4(4-Benzophenylazo)pyrogallol, through coupling pyrogallol with diazotized para-aminobenzoic acid in regulated pH medium, ranging between 5.0 to 6.0. The existence of common excipients in the dietary supplement did not produce any significant interference. F- and ttest data analysis were used for statistical comparison of the suggested techniques with that of reference method, demonstrating excellent agreement with no significant difference in the associated precision and accuracy.

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