scholarly journals Bioremediation of Heavy Metal Chrome with Saccharomyces cerevisiae in Industrial Metal Plating Liquid Waste

2018 ◽  
Author(s):  
Mr. Mardiyono ◽  
Nur Nur Hidayati ◽  
Nony Puspawati
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Liquid Waste ◽  
Metal Plating

scholarly journals VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 487
Author(s):  
Alexander Tomashevsky ◽  
Ekaterina Kulakovskaya ◽  
Ludmila Trilisenko ◽  
Ivan V. Kulakovskiy ◽  
Tatiana Kulakovskaya ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Stress Response ◽  
Stress Resistance ◽  
Alkaline Stress ◽  
Inorganic Polyphosphate ◽  
Divalent Metal Transporter ◽  
Metal Transporter ◽  
Elevated Expression ◽  
Chaperone Complex

Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg2+-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast.

scholarly journals Comparison of Heavy Metal Adsorption between Pseudomonas cepacia H42 and Saccharomyces cerevisiae SEY2102

2010 ◽  
Vol 19 (9) ◽  
pp. 1177-1185
Author(s):  
Ji-Won Park ◽  
Yu-Jeong Jeong ◽  
Eun-Ju Ryu ◽  
Byung-Woo Kim ◽  
Hyun-Ju Kwon ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Metal Adsorption ◽  
Pseudomonas Cepacia ◽  
Heavy Metal Adsorption

scholarly journals The Effectiveness Biosorption of Durian (Durio zibhetinus) Rind Pectin on Handling Liquid Waste Containing Heavy Metal (Pb II)

2018 ◽  
Vol 248 ◽  
pp. 04003
Author(s):  
Flora Elvistia Firdaus ◽  
Amida Redella ◽  
Sintani Nursabila
Keyword(s):  
Heavy Metal ◽  
Time Variation ◽  
Organic Waste ◽  
Liquid Waste ◽  
Extraction Time ◽  
Degree Of Esterification ◽  
Water Pollutants ◽  
Metal Binder ◽  
Living Things ◽  
Durian Rind

Heavy metal is one of the most substances occur in water pollutants. It is harmful to humans and other living things. The biosorption of pectin from durian rind is used as a heavy metal binder. However, the type of pectin presented in organic waste is generally HMP (High Methoxyl Pectin), which previously should be demethylation. Durian rind used in this study are originated from Bogor West Java. This study aims to determine the effectiveness of pectin biosorbent in reducing the concentration of heavy metal (Pb II). Pectin extraction was carried out at 80 °C within a time variation of 1; 5; and 6 hours. The results showed that the most optimum pectin is with 6 hours of extraction time where the methoxyl content is 3.46%; weight equivalent 3860 % galacturonate content; the degree of esterification 20.29%; and lead (Pb II) uptake 97%. While durian seeds do not contain pectin.

Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin

1992 ◽  
Vol 157 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Ralf Kneer ◽  
Toni M. Kutchan ◽  
Andreas Hochberger ◽  
Meinhart H. Zenk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Neurospora Crassa

scholarly journals Biosorption of heavy metal copper (Cu2+) by Saccharomyces cerevisiae

2018 ◽  
Vol 106 ◽  
pp. 012090
Author(s):  
S A J Ririhena ◽  
A D Astuti ◽  
M F Fachrul ◽  
M D S Silalahi ◽  
R Hadisoebroto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal

Vacuolar H + ‐ ATPase is involved in preventing heavy metal‐induced oxidative stress in Saccharomyces cerevisiae

2020 ◽  
Vol 22 (6) ◽  
pp. 2403-2418
Author(s):  
Todsapol Techo ◽  
Supat Jindarungrueng ◽  
Supinda Tatip ◽  
Tossapol Limcharoensuk ◽  
Prayad Pokethitiyook ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Heavy Metal

scholarly journals CzcD Is a Heavy Metal Ion Transporter Involved in Regulation of Heavy Metal Resistance in Ralstonia sp. Strain CH34

1999 ◽  
Vol 181 (22) ◽  
pp. 6876-6881 ◽  
Author(s):  
Andreas Anton ◽  
Cornelia Große ◽  
Jana Reißmann ◽  
Thomas Pribyl ◽  
Dietrich H. Nies
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Metal Ion ◽  
Constitutive Expression ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Cation Diffusion ◽  
Ion Efflux ◽  
Domains Of Life ◽  
Membrane Bound

ABSTRACT The Czc system of Ralstonia sp. strain CH34 mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. The CzcD protein is involved in the regulation of the Czc system. It is a membrane-bound protein with at least four transmembrane α-helices and is a member of a subfamily of the cation diffusion facilitator (CDF) protein family, which occurs in all three domains of life. The deletion ofczcD in a Ralstonia sp. led to partially constitutive expression of the Czc system due to an increased transcription of the structural czcCBA genes, both in the absence and presence of inducers. The czcD deletion could be fully complemented in trans by CzcD and two other CDF proteins from Saccharomyces cerevisiae, ZRC1p and COT1p. All three proteins mediated a small but significant resistance to cobalt, zinc, and cadmium in Ralstonia, and this resistance was based on a reduced accumulation of the cations. Thus, CzcD appeared to repress the Czc system by an export of the inducing cations.

scholarly journals Biosorption of Chromium (VI) and Calculations of Langmuir’s and Freundlichs Isotherms

2018 ◽  
Vol 60 (2) ◽  
pp. 90-95
Author(s):  
Muhammad Tahir Butt ◽  
Zara Amjad ◽  
Rauf Ahmad Khan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aqueous Solution ◽  
Heavy Metal ◽  
Toxic Metals ◽  
Low Cost ◽  
Cost Effective ◽  
Environmental Applications ◽  
Chromium Vi ◽  
Yeast Biomass ◽  
Process Cost

In the present study yeast biomass has been successfully used as biosorbent for removal of Crfrom aqueous solution. Yeasts of Saccharomyces cerevisiae are effective biosorbents for heavy metal ionsand it can be bought in large quantity at low cost. S. cerevisiae can remove toxic metals from aqueoussolutions to various levels. This low-cost biosorbent will make the process cost-effective and competitiveparticularly for environmental applications in detoxifying effluents. Langmuir’s and Freundlichs isothermswere also plotted to observe the maximum biosorption of heavy metal chromium (VI).

scholarly journals Biosorpsi logam Zn oleh biomassa Saccharomyces cerevisiae *) Biosorption of Zn metal by Saccharomyces cerevisiae biomass

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
. TRI-PANJI
Keyword(s):  
Waste Water ◽  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Industrial Waste ◽  
Contact Time ◽  
Sorption Process ◽  
Ion Concentration ◽  
Excess Base ◽  
Maximum Limit ◽  
Latex Rubber

SummaryHeavy metal in waste water potentiallycauses environmental pollution. Generally,heavy metal pollutions come from metalplating, textile, latex-rubber goods, and otherindustries. The process of latex-rubber goodindustries uses heavy metal in the form ofZnO as accelerator for rubber vulcanizationprocess, so that Zn 2+ ion exists in wastewatereffluents in concentration as much as 300 ppm,whereas the maximum limit allowed is 2.5 ppm.The chemical way generally used to decreaseZn 2+ concentration in wastewater effluents isby adding bases, NaOH or Ca(OH) 2 until pHreached 11, hence this metal is precipitated asits hydroxide. However, the way is done, isvery high cost and has a risk of the emergencesecondary pollution caused by excess base. Analternative way to absorb Zn 2+ consideredinexpensive is by using biosorbent in the formof Saccharomyces cerevisiae biomass frombioethanol industrial waste. The research wasconducted using artificial wastewater withZn 2+ ion concentration of 300 ppm and the pHwas adjusted to the range between 3-7.Biosorption was conducted by addition of freeS. cerevisiae biomass as well as byimmobilized cells on filter paper. Observationwas carried out for Zn 2+ concentration aftercontact time of two and five hours. The resultsof the research indicated that free andimmobilized S. cerevisiae biomass couldabsorb Zn 2+ metal and decreased itsconcentration from 250-300 ppm to 20-50 ppm.The optimum contact time was reached at onehour, while optimum sorption process occurredat pH 5. At low concentration, less than20 ppm S. cerevisiae biomass absorbed lessZn 2+ The NaOH-treated biomass showed bettersorption capabilities compared to cells treatedby formaldehyde or heat treatments. Thecontinue experiment showed the high capacityof biomass treated with NaOH to absorb Zn 2+ ,until concentration 24,02- 47,95 ppm in thefirst sampling and 1,15-10,99 ppm in thesecond sampling. Combination adsorptionprocess using charcoal and zeolite couldadsorp remain concentration of Zn 2+ , so thatcould reached the limit concentration-allowed.RingkasanLogam berat di dalam air limbahmerupakan penyebab pencemaran lingkunganyang potensial. Pencemaran logam berat padaumumnya berasal dari industri penyepuhanlogam, tekstil, barang jadi lateks, serta industrilain. Pada proses industri barang jadi lateksdigunakan logam berat dalam bentuk ZnOsebagai akselerator proses vulkanisasi karet,sehingga ion Zn 2+ terbawa dalam air limbahindustri barang jadi dengan konsentrasimencapai 300 ppm, sedangkan ambang bataskonsentrasi yang diperbolehkan maksimaladalah 2,5 ppm. Cara kimia yang umum di-gunakan untuk menurunkan kandunganZn 2+ dalam air limbah adalah dengan caramenambahkan basa, umumnya NaOH atauCa(OH) 2 , sampai pH sekitar 11, sehinggalogam berat ini diendapkan sebagai hidroksida-nya. Namun demikian, cara ini sangat mahaldan beresiko munculnya pencemaran sekunderakibat kelebihan basa. Salah satu alternatifyang murah untuk penyerapan Zn 2+ adalahmenggunakan biosorben berupa biomassaSaccharomyces cerevisiae yang berasal darilimbah pabrik bioetanol.Penelitian dilakukan dengan mengguna-kan air limbah artifisial yang mengandung ionZn 2+ dengan konsentrasi 300 ppm. Limbahartifisial diatur pHnya antara 3-7. Biosorpsidilakukan dengan menambahkan biomassaS. cerevisiae bebas maupun yang diamobilisasidengan kertas saring. Pengamatan dilakukanterhadap kandungan Zn 2+ setelah waktu kontakdua dan lima jam. Hasil penelitian menunjuk-kan bahwa biomassa S. cerevisiae bebasmaupun amobil mampu menyerap logam Zn 2+dan menurunkan konsentrasinya dari 250-300 ppm menjadi 20-50 ppm. Waktu kontakoptimum dicapai setelah satu jam, sedangkanproses sorpsi optimum terjadi pada pH 5.Biomassa S. cerevisiae kurang efektifmenyerap logam Zn 2+ pada konsentrasi rendah,di bawah 20 ppm. Perlakuan biomassa meng-gunakan NaOH menunjukkan kemampuanpenyerapan yang lebih baik jika dibandingkandengan yang diperlakukan menggunakanformaldehida dan pemanasan. Percobaan padaaliran kontinyu yang menggunakan biomassayang diperlaukan menggunakan NaOH,menunjukkan bahwa limbah artifisial Zn 2+dapat diturunkan sampai konsentrasi 24,02-47,95 ppm pada sampling pertama, dan 1,15-10,99 ppm pada sampling kedua. Kombinasipenyerapan menggunakan arang aktif danzeolit dapat menyerap sisa Zn 2+ mencapai batasyang diperbolehkan.

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