A High-Definition Imaging System Utilizing Single Lens And Double Monochromatic Light Sources

A method has been developed for densitometric estimation of the Feulgen-stained DNA content of 3H-labeled nuclei in autoradiographs in conjunction with automated grain counting using a Quantimet Imaging System. Refinements in the methodology are reported which include 1) the incorporation of an Image-Editor Module into the Quantimet module configuration; 2) the optimization of incident illumination based upon evaluation of various light sources; 3) changes in the optical configuration which reduce glare and minimize the level of monitor shading correction; 4) the optimization of scanner sensitivity; and 5) the evaluation of cell-flattening and staining with respect to densitometry resolution and sensitivity. These refinements resulted in a CV of less than 6.4% in the G-1 and G-2 DNA peaks of rat kidney cells in autoradiographs compared to the previous CV of 10.5%, and a G-2 to G-1 ratio of 2.025. For a fixed field position the CV was 5.1% and the replication error less than 1.0%.

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Super high definition imaging system in ATM network

Fifth International Conference on Image Processing and its Applications ◽

10.1049/cp:19950704 ◽

1995 ◽

Author(s):

R. Suzuki

Keyword(s):

Imaging System ◽

High Definition ◽

Atm Network

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High-Fluence Rate Monochromatic Light Sources, Computerized Analysis of Cell Movements, and Microbeam Irradiation of a Moving Cell: Current Experimental Methodology at the Okazaki Large Spectrograph

Biophysics of Photoreceptors and Photomovements in Microorganisms ◽

10.1007/978-1-4684-5988-3_26 ◽

1991 ◽

pp. 327-337 ◽

Cited By ~ 4

Author(s):

Masakatsu Watanabe

Keyword(s):

Monochromatic Light ◽

Experimental Methodology ◽

Light Sources ◽

Fluence Rate ◽

High Fluence ◽

Cell Movements ◽

Microbeam Irradiation ◽

Computerized Analysis ◽

Cell Current

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Beeporter: a high-throughput tool for non-invasive analysis of honey bee virus infection

10.1101/2021.01.13.426606 ◽

2021 ◽

Author(s):

Jay D. Evans ◽

Olubukola Banmeke ◽

Evan C. Palmer-Young ◽

Yanping Chen ◽

Eugene V. Ryabov

Keyword(s):

Virus Infection ◽

Honey Bee ◽

High Throughput ◽

Honey Bees ◽

High Throughput Screening ◽

Fluorescent Protein ◽

Imaging System ◽

Uv Light ◽

Light Sources ◽

Deformed Wing Virus

ABSTRACTHoney bees face numerous pests and pathogens but arguably none are as devastating as Deformed wing virus (DWV). Development of antiviral therapeutics and virus-resistant honey bee lines to control DWV in honey bees is slowed by the lack of a cost-effective high-throughput screening of DWV infection. Currently, analysis of virus infection and screening for antiviral treatments in bees and their colonies is tedious, requiring a well-equipped molecular biology laboratory and the use of hazardous chemicals. Here we utilize a cDNA clone of DWV tagged with green fluorescent protein (GFP) to develop the Beeporter assay, a method for detection and quantification of DWV infection in live honey bees. The assay involves infection of honey bee pupae by injecting a standardized DWV-GFP inoculum, followed by incubation for up to 44 hours. GFP fluorescence is recorded at intervals via commonly available long-wave UV light sources and a smartphone camera or a standard ultraviolet transilluminator gel imaging system. Nonlethal DWV monitoring allows high-throughput screening of antiviral candidates and a direct breeding tool for identifying honey bee parents with increased antivirus resistance. For even more rapid drug screening, we also describe a method for screening bees using 96-well trays and a spectrophotometer.

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Realized large field of view infrared imaging system of single lens with external field splicing

Infrared and Laser Engineering ◽

10.3788/irla202049.0314002 ◽

2020 ◽

Vol 49 (3) ◽

pp. 314002-314002

Author(s):

单秋莎 Qiusha Shan ◽

苏秀琴 Xiuqin Su ◽

段晶 Jing Duan ◽

周亮 Liang Zhou ◽

刘凯 Kai Liu ◽

...

Keyword(s):

External Field ◽

Infrared Imaging ◽

Imaging System ◽

Field Of View ◽

Large Field ◽

Infrared Imaging System ◽

Single Lens

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Photo-Electron Emission Microscopy of Semiconductor Surfaces

Microscopy and Microanalysis ◽

10.1017/s1431927600023151 ◽

1998 ◽

Vol 4 (S2) ◽

pp. 606-607

Author(s):

R.J. Nemanich ◽

S.L. English ◽

J.D. Hartman ◽

W. Yang ◽

H. Ade ◽

...

Keyword(s):

Electron Emission ◽

Imaging System ◽

Uv Light ◽

Ultra Violet ◽

Objective Lens ◽

Electron Optics ◽

Light Sources ◽

Emission Microscopy ◽

Electron Imaging ◽

Crucial Part

The technique of photo-electron emission microscopy (PEEM) is based on imaging of photo excited electrons from a surface. Typically ultra violet (UV) light above the work function of a metal will cause electrons to be emitted from a surface. Since photo excited electrons originate very near to the surface, they essentially reflect the electronic structure of the surface. These electrons may be accelerated and imaged, and the image will reflect the properties of the surface.While the PEEM technique has been understood in a basic sense for many years, it has been limited by the lack of high intensity UV light sources. The most crucial part of the electron imaging system for PEEM, the objective lens, is essentially the same as for the sister technique of low energy electron microscopy (LEEM), and advances in electron optics capabilities have been exploited both for LEEM and for PEEM.

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