scholarly journals THE RELATIONSHIP BETWEEN PRODUCTION OF PROTEIN A, CAPSULE STRUCTURE AND SOME ENZYMES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM INFECTIONS OF HUMAN AND ANIMALS

2021 ◽  
Vol 26 (1) ◽  
pp. 169-174
Author(s):  
Safana, A. S. AL-Taan ◽  
ASMAA, H. Al-Jobori
Keyword(s):  
Staphylococcus Aureus ◽  
Enzyme Production ◽  
Protein A ◽  
Capsule Formation ◽  
Negative Results ◽  
Best Value ◽  
Highly Correlated ◽  
The Relationship ◽  
Different Sources ◽  
Positive Results

Thirty-five isolates of Staphylococcus were isolated out of 100 clinical specimens were collected from different infections of human and animals. These isolates were identified as Staphylococcus aureus, 15 of them isolated from human, 15 from cows and only 5 isolates from poultry. Twenty-five (71.4%) isolates of different sources revealed positive results for the detection of protein A, capsule formation and enzyme production of phosphatase, DNase and haemolysin. Seven (20 %) isolates showed negative results for both protein A and capsule as well as for the production of enzymes while only three (8.5%) isolates were not capsulated but able to produce protein A and the enzymes. During the detection of protein A, capsule structure and production of some enzymes, we found that the best value of pH used was the range of 7-7.5. The present study indicated that the presence of protein A and capsule structure is highly associated with the virulence of isolates, and loss of protein A doesn't necessary mean loss of capsule as well. The ability of the isolates to produce their essential enzymes to induct the infection is highly correlated with the ability to produce protein A, without considering the source of the isolates.

PET/CT WITH 68GA-PSMA-11 IN DETECTION OF PRIMARY TUMOR AND RECURRENCE OF PROSTATE CANCER

2018 ◽  
Vol 64 (6) ◽  
pp. 799-804
Author(s):  
Darya Ryzhkova ◽  
M. Poyda
Keyword(s):  
Prostate Cancer ◽  
Primary Tumor ◽  
Biochemical Recurrence ◽  
Whole Body ◽  
Negative Results ◽  
Primary Prostate Cancer ◽  
Pet Ct ◽  
The Relationship ◽  
Positive Results ◽  
T Category

Purpose: To study the diagnostic value of PET-CT with 68Ga-PSMA-11 in the diagnosis of a primary prostate cancer, preoperative staging, and the detection of recurrence of prostate cancer (PCa). Methods: 28 patients aged 64.7 ± 8.74 years were included. 10 patients primary prostate cancer, and 18 patients with biochemical recurrence of the disease after radical treatment were examined. All patients underwent PET-CT with 68Ga-PSMA-11 according the whole body protocol. Interpretation of images was performed visually and quantitatively by calculation of SUL max. Results: High focal or diffuse 68Ga-PSMA-11 uptake was found in prostate parenchyma in patients with primary prostate cancer. Additionally metastases in regional lymph nodes were diagnosed in 4 patients and bone metastases were found in one patient. The correlation between 68Ga-PSMA-11 uptake level and Gleason index in the primary tumor (R Spearmen = 0.25, p = 0.57) was not observed. PET-positive results were obtained in 14 patients and PET-negative results in 4 patients with biochemical recurrence of PCa. The relationship between the frequency of PET-positive results and Gleason index was not revealed (R Spearmen = 0.2, p = 0.39). We found a weak but significant correlation between the frequency of PET-positive results and the prostate tumor stage according to the T category (R Spearmen = 0.49, p = 0.049). In patients with low values of PSA (less than 1.0 ng/ml) in 4 out of 9 cases, PET-negative results were obtained. In patients with PSA level more than 1.0 ng/ml PET-positive results were obtained in all cases. Conclusions: PET/CT with 68Ga-PSMA-11 allows to diagnose the primary prostate cancer, to establish the stage of the disease in categories N and M, and also to determine the localization and dissemination of the tumor in patients with biochemical recurrence of prostate cancer. The relationship between 68Ga-PSMA-11 uptake in primary tumor and Gleason index was not found. The probability of obtaining PET-positive results in cases of biochemical recurrence is affected by a PSA level above 1 ng/ml and a high stage of the disease according to the T category (T3-T4).

scholarly journals Clinical and laboratory features of typhoid fever in childhood

2001 ◽  
Vol 41 (3) ◽  
pp. 149 ◽  
Author(s):  
Sylvia Retnosari ◽  
Alan R. Tumbelaka ◽  
Arwin P. Akib ◽  
Sri Rezeki Hadinegoro
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Antibiotic Administration ◽  
Inclusion Criteria ◽  
Widal Test ◽  
Laboratory Findings ◽  
Sample Number ◽  
Negative Results ◽  
The Relationship ◽  
Positive Results

The aims of the study were to review the clinical features of patient with suspected typhoid fever, to obtain features of the blood culture results, Widal, Typhidot tests and to identify the relationship between/prior antibiotic administration with laboratory findings. The study had been conducted since January 1, 1999 till January 31, 2000 with inclusion criteria (1) age of patient was 3 to 14 years, (2) patient with diagnosis of suspected typhoid fever and (3) gave consent to participate in the present study. Thirty-six patients were eligible for this study and the result showed that typhoid fever was encountered more commonly in girls with the most prevalent onset was in age 5 to 9 years and duration of fever was less than 8 days. Gastrointestinal tract disturbance was the most frequent complain after fever. Most patient showed negative results in blood culture examination and Widal test, and gave positive results in Typhidot/Typhidot M test as well. Antibiotic administration tended to influence blood culture and Widal test as to need consideration in evaluation of laboratory results. However, a conclusion still could not be drawn definitively that further study with adequate sample number and positive blood culture as criteria inclusion was needed. 

Liquid Chromatography Verification of Tetracycline Residues in Milk and Influence of Milk Fat Lipolysis on the Detection of Antibiotic Residues by Microbial Assays and the Charm II Test

1992 ◽  
Vol 55 (5) ◽  
pp. 374-378 ◽  
Author(s):  
ASE CARLSSON ◽  
LENNART BJÖRCK
Keyword(s):  
Liquid Chromatography ◽  
Milk Fat ◽  
Causative Factor ◽  
Milk Samples ◽  
Negative Results ◽  
Highly Correlated ◽  
Routine Assay ◽  
Tetracycline Residues ◽  
Positive Results

Farm milk samples, which were positive in the routine assay for inhibitory substances detected by the Arla microtest but negative in the Delvotest SP, were used in the study. All samples analyzed gave positive results in the determination of tetracyclines and macrolides by the Charm II microbial receptor tests. To confirm the presence of tetracyclines, liquid chromatography and the Charm II immuno-receptor test were used. All samples, except one, showed negative results in the analyses by both these methods. This suggested that some other factor(s) was causing the observed positive results for tetracyclines and macrolides. Circumstantial evidence indicated that free fatty acids (FFA) may be a causative factor. Consequently, the influence of FFA on the Arla microtest, Valio T101, Delvotest SP, and the tetracycline and macrolide determinations by the Charm II test was investigated. Lipolysis of milk fat was induced by the addition of human blood serum, followed by storage for 24 and 48 h at 4°C. Samples were found to be inhibitory in the Arla microtest and the Valio T101 test at FFA levels of 4–5 mM, whereas the results of the Delvotest SP remained negative. The Charm II counts for tetracyclines and macrolides as determined by the microbial receptor tests were also highly correlated with FFA. It was concluded that lipolysis of milk fat may cause positive results in the microbial nonagar assays and interfere in the confirmation by the Charm II test.

Impact of Results of Methicillin-Resistant Staphylococcus, aureus Surveillance Culture of Nasal Specimens on Subsequent Antibiotic Prescribing Patterns

2010 ◽  
Vol 31 (8) ◽  
pp. 842-845
Author(s):  
Jörg J. Ruhe ◽  
Barry Kreiswirth ◽  
David C. Perlman ◽  
Donna Mildvan ◽  
Brian Koll
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Prescribing Patterns ◽  
Antibiotic Prescribing ◽  
Carrier Status ◽  
Surveillance Culture ◽  
Methicillin Resistant ◽  
Negative Results ◽  
Potential Impact ◽  
Positive Results

We studied the potential impact of results of methicillin-resistant Staphylococcus aureus (MRSA) surveillance culture of nasal specimens on physicians' vancomycin-prescribing habits. We compared 116 case patients who had positive results with 116 matched control subjects who had negative results. On multivariate analyses, a positive MRSA carrier status remained strongly predictive of vancomycin use within the subsequent 12 weeks.

The relationship of IGE receptor topography to signaling activity in RBL-2H3 mast cells

1991 ◽  
Vol 49 ◽  
pp. 236-237
Author(s):  
J.R. Pfeiffer ◽  
J.C. Seagrave ◽  
C. Wofsy ◽  
J.M. Oliver
Keyword(s):  
Mast Cells ◽  
Protein A ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Ige Binding ◽  
Electron Imaging ◽  
Small Clusters ◽  
Relationship Of ◽  
The Relationship

In RBL-2H3 rat leukemic mast cells, crosslinking IgE-receptor complexes with anti-IgE antibody leads to degranulation. Receptor crosslinking also stimulates the redistribution of receptors on the cell surface, a process that can be observed by labeling the anti-IgE with 15 nm protein A-gold particles as described in Stump et al. (1989), followed by back-scattered electron imaging (BEI) in the scanning electron microscope. We report that anti-IgE binding stimulates the redistribution of IgE-receptor complexes at 37“C from a dispersed topography (singlets and doublets; S/D) to distributions dominated sequentially by short chains, small clusters and large aggregates of crosslinked receptors. These patterns can be observed (Figure 1), quantified (Figure 2) and analyzed statistically. Cells incubated with 1 μg/ml anti-IgE, a concentration that stimulates maximum net secretion, redistribute receptors as far as chains and small clusters during a 15 min incubation period. At 3 and 10 μg/ml anti-IgE, net secretion is reduced and the majority of receptors redistribute rapidly into clusters and large aggregates.

Do Extra-Platelet Sources Contribute to the Plasma Level of Thrombospondin?

1988 ◽  
Vol 59 (02) ◽  
pp. 273-276 ◽  
Author(s):  
J Dawes ◽  
D A Pratt ◽  
M S Dewar ◽  
F E Preston
Keyword(s):  
Platelet Count ◽  
Background Concentration ◽  
Serum Concentrations ◽  
Bone Marrow Depression ◽  
Serial Sampling ◽  
Control Serum ◽  
Platelet Release ◽  
Highly Correlated ◽  
The Relationship

SummaryThrombospondin, a trimeric glycoprotein contained in the platelet α-granules, has been proposed as a marker of in vivo platelet activation. However, it is also synthesised by a range of other cells. The extraplatelet contribution to plasma levels of thrombospondin was therefore estimated by investigating the relationship between plasma thrombospondin levels and platelet count in samples from profoundly thrombocytopenic patients with marrow hypoplasia, using the platelet-specific α-granule protein β-thromboglobulin as control. Serum concentrations of both proteins were highly correlated with platelet count, but while plasma β-thromboglobulin levels and platelet count also correlated, there was no relationship between the number of platelets and thrombospondin concentrations in plasma. Serial sampling of patients recovering from bone marrow depression indicated that the plasma thrombospondin contributed by platelets is superimposed on a background concentration of at least 50 ng/ml probably derived from a non-platelet source, and plasma thrombospondin levels do not simply reflect platelet release.

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