scholarly journals Effect of uses of Probiotic prepared from Lactobacillus acidophilus Bacteria as immunostimulator in lambs vaccinated with Brucella melitensis Rev-1 vaccine

2009 ◽  
Vol 33 (1) ◽  
pp. 24-37
Author(s):  
Yousif A. A.
Keyword(s):  
Immune Response ◽  
Lactobacillus Acidophilus ◽  
Cellular Immune Response ◽  
Protein Concentration ◽  
Brucella Melitensis ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Immunological Tests ◽  
Cellular Immune ◽  
Positive Results

This study was conducted to investigate the effect of probiotic prepared from Lactobacillus acidophilus & usedas immunostimulator to improve the humeral &cellular immune response in lambs vaccinated with the Rev-1 vaccine.Lactobacillus isolated from intestinal contents of sheep after culturing on specific media &study the morphological &biochemical characteristic Lactobacillus acidophilus was selected as probiotic after definitive diagnosis and dependingon their higher adherence ability to epithelial cells of the intestine and have high tolerance to low pH and bile salts .thensensitivity test of the strain against antibiotics was done The experimental study included 15 lambs (age 5-8 months)were equally divided into three groups and treated as following.* First group was vaccinated with Rev -1 vaccine (1ml contain 2×109CFU- s/c as single dose ) and ioculated orally withprepared probiotic (2 ×109 CFU /lamb ) at one week before vaccination and continued daily to end of experiment(14weeks).*Second group was vaccinated only with Rev -1 vaccine at same way in first group. *Third group was inoculated with 1ml PBS S\c as control group. Clinical and immunological tests were conducted to all lambs at 0 times & after 2, 6, 10, 14weeks. The results of clinical tests revealed that there is increase in body temperature, respiratory &pulse rates ofvaccinated animals only but the second group show the highest parameters. The vaccinated lambs shows humeralimmune response by giving a positive results to rose Bengal test & by using Passive haemagglutination test, the firstgroup revealed high significant antibody titer (716.80±125.413) than the second group and third group (p<0.05 ) . andthe results of cellular immune response, as detected by delayed type hypersensitivity test which give a positive results invaccinated groups but the first group was significantly higher (p<0.05 )at than rates in redness area and thicknesscompared with the second group E. rosette test showed significant increase at(p<0.05 ) of activated lymphocyte infirst group ( 51.976±4.619 )compared with second (41.22±3.6865). Serum total protein concentration was increased infirst group (9.7358±0.2615) compared with other groups.

scholarly journals BEHAVIORAL AND IMMUNOLOGICAL EFFECTS OF EXPOSURE TO ARSENIC AND ANTIMONY CONTAINED IN SULFIDE ORE PROCESSING WASTE

2021 ◽  
Vol 4 (2) ◽  
pp. 133-139
Author(s):  
Alexander S. Ogudov ◽  
Natalia F. Chuenko ◽  
Maria A. Knyazheva ◽  
Lyudmila Yu. Anopchenko
Keyword(s):  
Immune Response ◽  
Drinking Water ◽  
Cellular Immune Response ◽  
Delayed Type Hypersensitivity ◽  
Laboratory Animals ◽  
Control Group ◽  
Free Access ◽  
Behavioral Reactions ◽  
Experimental Animals ◽  
Cellular Immune

The accumulation of arsenic and antimony compounds in environmental objects as a result of the development of mineral deposits is a source of public health risk. Arsenic and antimony, when they enter the human body in excess, activate pathophysiological processes at the subcellular, cellular and organ levels, which leads to the development of multiple organ pathology. The study confirmed the effect of arsenic and antimony on emotional-behavioral responses and cellular immune responses in laboratory animals. Four experimental groups of male Wistar rats and one control group were formed. Experienced with free access to drinking water with different concentrations of arsenic and antimony (water from the hydro dump of the sulphide tailing dump in the village of Komsomolsk, Kemerovo region), the control had free access to clean drinking water. On the 7th and 70th days of the experiment, the emotional-behavioral reactions of the experimental animals were investigated in the "open field" and "raised cruciform maze" tests. The assessment of the cellular immune response was performed by the delayed-type hypersensitivity reaction (HRT). Arsenic and antimony in the studied concentrations have a harmful effect on the central nervous system (CNS) and the cellular immune response of experimental animals, which manifests itself in disorganization of emotional-behavioral reactions, induction of delayed-type hypersensitivity reactions.

Effect of Hypertonic Saline Infusion on Postoperative Cellular Immune Function

2004 ◽  
Vol 100 (5) ◽  
pp. 1108-1118 ◽  
Author(s):  
Jens A. Kølsen-Petersen ◽  
Jens-Ole D. Nielsen ◽  
Else M. Tonnesen
Keyword(s):  
Immune Response ◽  
Immune Function ◽  
Hypertonic Saline ◽  
Cellular Immune Response ◽  
Killer Cell ◽  
Cell Activity ◽  
Abdominal Hysterectomy ◽  
Delayed Type Hypersensitivity ◽  
Laboratory Animals ◽  
Cellular Immune

Background Previous studies found hypertonicity to affect immune responses in intact laboratory animals and in human blood cell cultures. In this study, the authors investigated the cellular immune response to surgery after preoperative infusion of hypertonic saline in humans. Methods Sixty-two women scheduled to undergo abdominal hysterectomy were randomly assigned to single-blinded infusion of 4 ml/kg NaCl, 7.5%; 4 ml/kg NaCl, 0.9%; or 32 ml/kg NaCl, 0.9%, over 20 min. Blood was collected at baseline, during surgery, and 1, 24, and 48 h after surgery for the determination of leukocyte and differential counts, flow cytometric phenotyping of mononuclear cells, and natural killer cell activity against K 562 tumor cells. Phytohemagglutinin-induced lymphocyte proliferation, plasma elastase, and neutrophil chemotaxis were measured at the same time points except during surgery. The authors tested cell-mediated immune function in vivo by delayed-type hypersensitivity reaction in the skin. Results Surgery induced well-known changes in the cellular immune response, which were unrelated to the tonicity or volume of the infused fluids. Conclusion Infusion of a clinically relevant dose of hypertonic saline did not seem to modify the postoperative cellular immune response after elective abdominal hysterectomy.

scholarly journals How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae

2021 ◽  
Vol 7 (11) ◽  
pp. 950
Author(s):  
Thaís Almeida Corrêa ◽  
Jéssica Fiorotti ◽  
Emily Mesquita ◽  
Laura Nóbrega Meirelles ◽  
Mariana Guedes Camargo ◽  
...  
Keyword(s):  
Immune Response ◽  
Metarhizium Anisopliae ◽  
Cellular Immune Response ◽  
Tick Control ◽  
Rhipicephalus Microplus ◽  
Phagocytic Index ◽  
Control Group ◽  
Immune Functions ◽  
Phenoloxidase Activity ◽  
Cellular Immune

Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of Rhipicephalus microplus inoculated with M. anisopliae blastospores. Exogenous DA increased both ticks’ survival 72 h after M. anisopliae inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with M. anisopliae did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.

scholarly journals Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

2021 ◽  
Vol 22 (6) ◽  
pp. 1111-1120
Author(s):  
I. A. Goldina ◽  
E. V. Markova ◽  
I. A. Orlovskaya ◽  
L. B. Toporkova ◽  
V. A. Kozlov
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Cellular Immune Response ◽  
Proliferative Activity ◽  
Lymphoid Organs ◽  
Delayed Type Hypersensitivity ◽  
Relative Mass ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem ◽  
Cellular Immune

Our aim was to evaluate immunomodulatory properties of an original bioflavonoid complex in experimental immune disturbances induced by cyclophosphamide (Cy). We have studied morphometric indexes of thymus and spleen, as well as blood leukocyte counts, cell proliferative activity in lymphoid organs, delayed hypersensitivity responses to T cell-dependent antigen, along with differentiation activity of bone marrow stem cells in experimental animals during Cy-induced immune suppression after a course of bioflavonoid treatment. Suspension of the bioflafonoid complex was introduced to the male mice (СВАхC57Bl/6)F1 aged 12- 14 weeks at a daily dose of 2 mg/animal (80 mg/kg), per os, using gastric catheter, over 14 days. Cytostatic immunosuppression was produced by a single intraperitoneal Cy injection. Proliferative activity of spleen and thymic cells was determined by standard method with Н3 -thymidine incorporation in the 72-h cell culture. Cellular immune response was assayed by the degree of delayed-type hypersensitivity development in response to sheep erythrocytes. The number of hematopoietic progenitors was evaluated by culturing bone marrow cells in methylcellulose-based medium. The experiments have shown mitigation of immunosuppressive effects induced by Cy, in the course of bioflavonoid complex treatment, with respect to absolute and relative mass of lymphoid organs and leukocyte numbers in peripheral blood. Moreover, we have demonstrated decreased effects of Cy treatment upon the spontaneous activity of spleen cells, mitogen-induced thymocyte and splenocyte proliferation, intensivity of delayed-type hypersensitivity response that reached the values of intact animals. Following the course of bioflavonoids, we have revealed an increase in early hematopoietic progenitors. Alleviation of Cy-induced suppressive effects upon cellular immune response, proliferation rates of immune cells, as well as stimulation of hematopoietic stem cell functions suggest a sufficient capacity of the original bioflavonoid complex for modulation of immunity and hematopoiesis, thus presenting experimental proofs for its potential usage as an adjuvant treatment of the patients with malignant diseases.

scholarly journals Immunomodulating properties of the probiotic preparation, VedaBiotic in aging

2021 ◽  
pp. 41-54
Author(s):  
Е.V. Markova ◽  
◽  
О.V. Sorokin ◽  
I.А. Goldina ◽  
I.V. Savkin ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Nutritional Supplement ◽  
Progressive Increase ◽  
Delayed Type Hypersensitivity ◽  
Elderly Persons ◽  
Cytostatic Treatment ◽  
Freeze Dried ◽  
Age Dependent ◽  
Cellular Immune

The homeostatic immunological dialogue between the host and its microbiota is critical to maintaining health. The weakening of the organism occurring with age, its greater dependence on the external life conditions, make old age the period of the greatest morbidity. In the context of a progressive increase in life expectancy, more and more attention is being paid to the possibility of influencing human health through personalized nutritional and therapeutic strategies aimed at modifying the intestinal microbiota, which include the use of pro- and prebiotics. The aim of this work is to study the effect of the VedaBiotic preparation, which is an original complex of probiotics (freeze-dried biomass of live active bifidobacteria, lactobacilli, Streptococcus thermophilus), prebiotics (microcrystalline cellulose, lactulose) and vitamin C, on the cellular immune response and functional activity of immunocytes in age-dependent mammals (male mice at the age of 10 months). It was found that in age-dependent mammals, a course administration of the drug leads to the stimulation of the proliferative activity of immunocytes and the cellular immune response, also including the case of the induced immunosuppression (when the delayed-type hypersensitivity reaction increased to the level observed in intact animals of similar age). The VedaBiotic also has a modulating effect on the cytokines production, causing a decrease in the pro-inflammatory cytokines IL-1β and IFN-γ, and an increase in the anti-inflammatory cytokine IL-10. Thus, the VedaBiotic preparation has a favorable immunomodulatory effect and can be used as a nutritional supplement — as a source of probiotic microorganisms — to reduce the negative side effects of cytostatic treatment, as well as by elderly persons to improve the quality of life and to prevent the development of age-associated pathology.

scholarly journals TURMERIC EXTRACT IN CORRECTION OF NERVOUS AND IMMUNE SYSTEMS FUNCTIONAL ACTIVITY PARAMETERS IN EXPERIMENTAL ALCOHOLISM

2019 ◽  
Vol 19 (1S) ◽  
pp. 215-217
Author(s):  
E V Markova ◽  
I A Goldina ◽  
B G Goldin ◽  
M A Knyazheva ◽  
I V Savkin
Keyword(s):  
Immune Response ◽  
Functional Activity ◽  
Cellular Immune Response ◽  
Experimental Models ◽  
Ethanol Solution ◽  
Delayed Type Hypersensitivity ◽  
Experimental Alcoholism ◽  
Immune Systems ◽  
Turmeric Extract ◽  
Cellular Immune

The purpose of this work was to study the effect of turmeric extract on behavior indicators, the severity of the cellular immune response in animals in a state of experimental alcoholism. Experimental models: mouse males (CBAxC57Bl/6)F1 three months of age (n = 60). Alcohol dependence in experimental animals was formed by the method of 6-month soldering with a 10% ethanol solution. In the control groups, the animals received per os water or 10% ethanol solution, in the experimental group - an extract of turmeric powder in a solution of ethanol. Mice behavior was assessed in the “open field” test. The severity of the cellular immune response to sheep erythrocytes was assessed by the intensity of the development of a delayed-type hypersensitivity reaction.It was found that the use of turmeric extract against the background of taking ethanol solution in animals with experimental alcoholism leads to the stimulation of behavior and the increase of the cellular immune response to the level characteristic of healthy animals of the corresponding age.Results indicates the protective effect of turmeric on a number of parameters of the functional activity of the nervous and immune systems during chronic ethanol intoxication.

scholarly journals Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge

2012 ◽  
Vol 93 (7) ◽  
pp. 1556-1562 ◽  
Author(s):  
Yeonsu Oh ◽  
Hwi Won Seo ◽  
Kiwon Han ◽  
Changhoon Park ◽  
Chanhee Chae
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Delayed Type Hypersensitivity ◽  
Porcine Circovirus ◽  
Newborn Piglets ◽  
Specific Cellular Immune Response ◽  
Cellular Immune

The objective of the present study was to evaluate (i) the passive transfer of maternally derived functional porcine circovirus type 2 (PCV2)-specific lymphocytes of seronegative sows immunized with the PCV2 vaccine to newborn piglets and (ii) the functional role of the maternally derived PCV2-specific cellular immune response in protecting newborn piglets from challenge with PCV2. After ingesting colostrums, piglets from vaccinated sows (PT01 and PT02) have significantly higher numbers of PCV2-specific gamma interferon-secreting cells, an increased PCV2-specific delayed type hypersensitivity response, and a stronger proliferative response of peripheral blood mononuclear cells compared with piglets from non-vaccinated seronegative sows (PT03 and PT04). In the PCV2 challenge study, the number of serum genomic PCV2 copies was significantly less in piglets from vaccinated sows (PT02) compared with piglets from non-vaccinated sows (PT04) at 7–28 days post-inoculation (P<0.05 and P<0.001). The histopathological lesions and immunohistochemical scores were significantly lower in piglets of vaccinated sows compared with those of non-vaccinated sows. To our knowledge, this is the first report of transferring a maternally derived PCV2-specific cellular immune response from vaccinated dams to their offspring. Maternally derived adaptive cellular immune responses play a critical role in protecting newborn piglets challenged with PCV2 at 3 weeks of age.

The prevalence of immediate and delayed type hypersensitivity reactions to Microsporum canis antigens in cats

2003 ◽  
Vol 5 (3) ◽  
pp. 161-166 ◽  
Author(s):  
KA Moriello ◽  
DJ DeBoer ◽  
J Greek ◽  
K Kuhl ◽  
M Fintelman
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Spontaneous Recovery ◽  
Delayed Type Hypersensitivity ◽  
Microsporum Canis ◽  
Hypersensitivity Reactions ◽  
Intradermal Testing ◽  
Cellular Immune ◽  
Culture Positive ◽  
Culture Negative

Spontaneous recovery from Microsporum canis infections in cats is thought to be dependent on the development of a competent immune response. The purpose of this study was to determine the prevalence of positive delayed type hypersensitivity reactions in cats with and without dermatophytosis. Four groups of cats were intradermally skin tested with M canis extract and test sites were evaluated both subjectively and objectively at 0, 24 and 48 h after injection. Delayed intradermal testing (IDT) reactions were absent in cats not exposed to dermatophytosis ( n=20); infected–recovered cats ( n=38 culture negative lesion negative and n=43 lesion negative but culture positive) had significantly larger IDT reactions than unexposed cats and cats that were still actively infected ( n=18). Based on the results of this study, IDT with M canis extract can be used to assess the cellular immune response of cats with dermatophytosis.

scholarly journals 22 Study the Immune Response of Conjunctival and Subcutaneous Vaccination of Goats with RB51 Vaccine for Controlling Brucellosis

2013 ◽  
Vol 37 (1) ◽  
pp. 22-28
Author(s):  
Waffa Abdulelah Ahmad1
Keyword(s):  
Immune Response ◽  
Skin Test ◽  
Serological Test ◽  
Optimal Concentration ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Skin Thickness ◽  
Skin Reactions ◽  
Passive Hemagglutination ◽  
Cellular Immune

This study was conducted to evaluate the efficacy of RB51 vaccine in immunization of goats against brucellosis although it is a specific vaccine for cattle, fifteen pregnant and non-pregnant goats were divided into three groups, 5 goats were injected subcutaneously(S/C) with 2 mls of RB51 vaccine which contain 4 × 108 CFU , 5 goats were vaccinated through conjunctival route (C/J) with 0.2 ml of RB51 which contain 4 × 108 CFU , and the last 5 goats were injected with PBS and kept as control group. It has been ascertained that no abortion occurred in the vaccinated animals except one case of a weak kid was born in S/C vaccinated group. Humeral immune response for the vaccinated animals measured by serological test (Rose Bengal and Passive hemagglutination tests) every two weeks, while the cellular immune response measured by brucellin test at the 6th week. Passive haemaglutination test (PHA) was positive and the antibodies were increased significantly (P<0.05) at the 2nd week to reach 10.4±2.4 and 6.4±0.9 in S/C and C/J routes respectively, and increased significantly (P<0.05) at 8th week to reach 38.4±6.4 and 22.4±3.9 in S/C and C/J routes respectively, and decreased significantly at 12th week which reach 9.6±1.6 to 6.4±0.9. Brucellin from RB51 strain prepared to be used as antigen in the PHA test and also in skin test to determine delayed type hypersensitivity, serial dilutions of RB51 brucellin had been done to confer the optimal concentration in skin test that did not induce toxicity for mice 40µg was the optimal concentration that has been given to goats. In goats the results indicated that the S/C group showed a high delayed type hypersensitivity compared with C/J group and the good skin reactions was increased to reach the maximum at 48 hrs post brucellin injection (PBI), when the erythema diameter reached 7.1±0.09mm and 5.4±0.3mm in S/C and C/J groups respectively and skin thickness was2.9±0.1 mm and 2.1±0.2 mm in S/C and C/J groups respectively. These results showed that immunized animals expressed cellular and humeral immune response examined by DTH and PHA. Concluded that the RB51 vaccine gave good level of immunization in goats and it can be considered as alternative vaccine against heterogonous Brucella spp.

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