scholarly journals Cytotoxic Effect of Datura stramonium Extract on Cancer Cell lines

2010 ◽  
Vol 34 (1) ◽  
pp. 131-138
Author(s):  
Ala'a H. Merza
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Datura Stramonium ◽  
Cancer Cell Lines ◽  
Brain Cell ◽  
Mammary Adenocarcinoma ◽  
Normal Rat ◽  
Embryonic Fibroblast

In order to investigate the in vitro antitumor activity of Datura stramonium seeds on cancer cell lines. Extract of this plant was prepared by using different concentrations of seed extract 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 and 5 mg/ ml. Cytotoxicity was estimated on mammary adenocarcinoma (AMN3), brain cancer, and normal rat embryonic fibroblast (Ref3) cell lines. The results exhibited that the extract has cytotoxic effect by decreasing the viability of AMN3 (42.91%) and brain cell lines (32.79%). However, it produced little effect on viability of normal cell line Ref3, indicating the specificity of this extract against malignant cells

scholarly journals NEW QUINAZOLINONE DERIVATIVES: SYTHESIS AND IN VITRO CYTOTOXIC ACTIVITY

2020 ◽  
Vol 58 (1) ◽  
pp. 12
Author(s):  
Tran Khac Vu
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Cancer Cell Lines ◽  
Ic50 Values ◽  
Quinazolinone Derivatives ◽  
Bioassay Result ◽  
Mcf 7

The paper presents a simple synthesis of new quinazolinone derivatives 13a-i. Synthesized derivatives were tested for their cytotoxic effect against three cancer cell lines including SKLU-1, MCF-7 and HepG-2. The bioassay result showed that only compound 13e exhibited significant cytotoxic effect against cancer cell lines tested with IC50 values of 9.48, 20.39 and 18.04 µg/ mL, respectively.

scholarly journals An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2049 ◽  
Author(s):  
Przemysław Sitarek ◽  
Ewelina Synowiec ◽  
Tomasz Kowalczyk ◽  
Tomasz Śliwiński ◽  
Ewa Skała
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Treatment Success ◽  
Cancer Cell Lines ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Cytotoxic Activities ◽  
Root Extract ◽  
Root Extracts

As the current cancer treatment success rate is not sufficient, interest has grown in plants as possible sources of anti-cancer compounds. One such plant with a broad spectrum of activity is Lenourus sibiricus of the family Lamiaceae. This study investigates for the first time both the genotoxic and cytotoxic activities of TR (transformed) and AtPAP1 TR (with over-expression of transcriptional factor) root extracts of Lenourus sibiricus against various cancer cell lines (CCRF-CEM, K-562 and A549). Both tested extracts showed a cytotoxic effect on CCRF-CEM and K-562 cell lines, but strongest activity was observed for the AtPAP1 TR extract. No cytotoxic effect was observed against the A549 cell line in the tested concentration range, and it was found that both tested extracts may induce apoptosis by decreasing mitochondrial membrane potential and inducing nDNA damage lesion in the TP53 region and mtDNA in ND1 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1) and ND5 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5) regions in K-562 and CCRF-CEM. Our results confirmed that TR and AtPAP1 TR root extracts from L. sibiricus are cytotoxic and genotoxic against different model cell lines (CCRF-CEM and K-562). However, the observed genotoxicity of both extracts needs to be confirmed by additional studies. These preclinical observations support the use of L. sibiricus with other pharmacological purposes.

scholarly journals SB365, Pulsatilla Saponin D Induces Caspase-Independent Cell Death and Augments the Anticancer Effect of Temozolomide in Glioblastoma Multiforme Cells

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3230 ◽  
Author(s):  
Jun-Man Hong ◽  
Jin-Hee Kim ◽  
Hyemin Kim ◽  
Wang Jae Lee ◽  
Young-il Hwang
Keyword(s):  
Cell Death ◽  
Glioblastoma Multiforme ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cathepsin B ◽  
Cytotoxic Effect ◽  
Cancer Cell Lines ◽  
Autophagic Flux

SB365, a saponin D extracted from the roots of Pulsatilla koreana, has been reported to show cytotoxicity in several cancer cell lines. We investigated the effects of SB365 on U87-MG and T98G glioblastoma multiforme (GBM) cells, and its efficacy in combination with temozolomide for treating GBM. SB365 exerted a cytotoxic effect on GBM cells not by inducing apoptosis, as in other cancer cell lines, but by triggering caspase-independent cell death. Inhibition of autophagic flux and neutralization of the lysosomal pH occurred rapidly after application of SB365, followed by deterioration of mitochondrial membrane potential. A cathepsin B inhibitor and N-acetyl cysteine, an antioxidant, partially recovered cell death induced by SB365. SB365 in combination with temozolomide exerted an additive cytotoxic effect in vitro and in vivo. In conclusion, SB365 inhibits autophagic flux and induces caspase-independent cell death in GBM cells in a manner involving cathepsin B and mainly reactive oxygen species, and its use in combination with temozolomide shows promise for the treatment of GBM.

scholarly journals The Effect of Crude Extracts of Sonchus oleraceus on Cancer Cell Growth (In vitro)

2010 ◽  
Vol 34 (2) ◽  
pp. 30-38
Author(s):  
Zainab R. Zghair
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Cell Types ◽  
Cancer Cell Lines ◽  
Mammary Adenocarcinoma ◽  
Sonchus Oleraceus

This study was designed to evaluate the anticancer, effects of the ethanolic (EE), cold aqueous (CAE), and hot aqueous (HAE) extracts of Sonchus oleraceus on cancer cell lines (in vitro). In vitro study was performed on three cancer cell lines (murine mammary adenocarcinoma AMN-3 cell line, laryngeal carcinoma Hep-2 cell line) and rat embryogenic fibroblast (REF) as normal cell line. Periods of exposure of cell lines were measured at 24, 48, and 72-hr in a microtitration plate under complete sterile conditions. Different concentrations starting from (78.125 to 10000) μg/ml of two fold dilution for each extract were prepared and tested on each cell line, with three replicates for each concentration. The three extracts showed concentration and time dependence with growth inhibitory effects, and the highest effect was obtained from ethanolic extract at higher concentrations after 48 hr. of exposures on both AMN3 and Hep-2 cell lines, while the cytotoxic effect of both cold aqueous and hot aqueous extracts on AMN-3 and Hep-2 cell lines exhibited that the higher concentrations gave a significantly (P<0.05) and the higher inhibition growth rate of cells were increased at 24 hrs.Conclusion: These results suggest that the cytotoxic concentrations of Sonchus oleraceus extracts showed variation in values among cell lines according to cell types in vitro.

scholarly journals Cytotoxic and apoptotic effects of cyproterone acetate against cancer cells and normal cells

2019 ◽  
Vol 13 (1) ◽  
pp. 68-74
Author(s):  
Hala M. Al-Saily ◽  
Mohammad Al-Halbosiy ◽  
Faris N. Al-Hady
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cyproterone Acetate ◽  
Cytotoxic Effect ◽  
Cell Function ◽  
Cancer Cell Lines ◽  
Anticancer Activities ◽  
Natural Product Research

Back ground: Cyproterone acetate (CPA) is a steroidal anti-androgen inhibits the testosterone  and  DHT action it is used as a medicine for prostate cancer by association with the androgen receptors located on the surface of prostate cells, thus preventing the association of testosterone with receptors. Objective: Investigation of the anticancer activities of Cyproterone acetate   against cancer cell lines testicular (Tera-1), macrophage (RAW 264.7)  in comparison to non- tumorigenic fetal hepatic cell line (WRL-68). Material and method: The cytotoxic effect of CPA was investigated according to selected parameters including: MTT assay as assay of cell function to determined cell viability, high content screening (HCS) technique for the apoptosis of cell. Only the most cytotoxic concentration of CPA and the most sensitive cells  as assayed by MTT was selected to complete the other test: (HCS). (MTT) assay was carried out at the Centre of Biotechnology Research’s, Al- NahrainUniversityBaghdad,Iraq . The HCS assay was performed at the Centre for Natural Product Research and Drug Discovery, Department of Pharmacology,Faculty of Medicine, University of Malaya, KualaLumpur, Malaysia Results: The most significant cytotoxic effect of Cyproterone acetate towards 2 cancer cell lines was obtained when its concentration was 1.25mg/mL. The Tera-1 Cells were more sensitive to Cyproterone acetate compared with RAW264.7  and  WRL-68. cells. There was a significant decrease  in valid cell count, nuclear intensity and mitochondrial membrane potential (MMP) when CPA (400 μg/mL)was used compared with doxorubicin (20 μg/mL) as a standard. Also, there was a significant increase in membrane permeability and cytochrome C releasing when CPA (400μg/ml) was used compared with positive control. Conclusion: CPA showed cytotoxic effects against the Tera-1 and RAW264.7  cancer cell lines while the WRL-68. Cells was not affected as determined in-vitro by the MTT assay. The HCStechnique also showed toxic effect towards Tera-1.

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