scholarly journals The immune response of broiler chickens fed diets supplemented with Propolis and Digestarom under heat stress condition

2019 ◽  
Vol 42 (2) ◽  
pp. 33-40
Author(s):  
Hasanain shauqi H.
Keyword(s):  
Immune Response ◽  
Heat Stress ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Stress Group ◽  
Lymphocyte Ratio ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Group 3

                                                             Summary    This research was carried out to evaluate the effect of dietary supplementation  of a mixture of  propolis and phytogenic (Digestarom)on immune responses against Newcastle disease and infectious bursal disease , also Heterophil/lymphocyte ratios in vaccinated broiler under thermoneutral (maintained on usual heat program)and heat stress maintained in  )33 ± 2°C). total of three hundred, one-day-old broilers (Rose 308) chicks were distributed equally in two separated room, thermoneutral  groups and heat stress HS groups along the duration of the experiment (42)day, then each group subdivided in to five groups( 30 chicks for each )  as follow: (thermoneutral group and heat stress group )fed a basal diet supplemented with propolis (2g/kg of diet),(thermoneutral group 2and heat stress2)fed Digestarom(150mg/kg).( thermoneutral  group 3 and heat stress group 3) fed a mixture of (propolis2g/kg +Digestarom150mg/kg) and two control groups without additive. Antibody titers against Newcastle disease  and infectious bursal disease   were measured and Heterophil/lymphocyte  ratio was estimated. In heat stress  chickens the results revealed a significant decrease(P<0.05)in immune response with significant increased(P<0.05) in Heterophil/lymphocyte  ratio, while higher significant (P<0.05)in antibody titers with significant decrease (P<0.05) in Heterophil/lymphocyte  ratio was showed in all thermoneutral chickens but more significant in thermoneutral group 3 and heat stress group 3 groups were gave the best values in comparing with others groups. The role of Propolis or phytogenic on broiler health and immunity had already been reported, but in this study was reported the effects of their mixture on ameliorating  deleterious effects of heat stress.

scholarly journals Effects of soluble ß-glucan on the immune responses of broiler chickens vaccinated with Newcastle disease vaccine and reared under heat stress

2014 ◽  
Vol 38 (1) ◽  
pp. 30-39
Author(s):  
Nidhal Raoof Mahdi
Keyword(s):  
Immune Response ◽  
Drinking Water ◽  
Monoclonal Antibodies ◽  
Heat Stress ◽  
Antibody Titer ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Positive Staining ◽  
Lymphocyte Ratio ◽  
Stressed Group

The main objectives of this study was to determine the influence of soluble β-glucan extracted from the cell wall of Saccharomyces cerevisiae on immune response of broiler chickens reared under heat stress .β-glucan 225μg/ml was supplemented in drinking water to broiler chicken vaccinated with Newcastle disease virus (NDV) vaccine. The parameters of the assessment of the immune response was the Heterophil / Lymphocyte ratio as a measure of stress, determination of the serum antibody titer post vaccination with the NDV vaccine by ELISA test and Immunohistochemical detection of macrophages by using monoclonal antibodies (mouse antichicken macrophage KUL01). A hundred and twenty (120) Chickens one day old were divided into two equal groups; group under heat stress and group control; each group was divided into two subgroups (G1, G2, G3 and G4) containing thirty chicks. The experiment was conducted for six weeks. The stressed group exposed to heat stress (≃35ºC) starting from the third week of age upto the end of the experiment. While (Group 1) and (G3) chicks were supplemented with 225µg/ml of soluble β-glucan in drinking water from day 1 to the end of the experiment, while (G2 and G4) chicks were not supplemented. The result of Heterophil /Lymphocyte ratio indicates that there was a significant (P < 0.05) difference within heat stressed treated which showed an elevated H/L ratio at 21,28 and 35 days old. Also there was a significant (P < 0.05) difference between groups that were treated with β-glucan (G1, G3) at 21, 28 and 32 days of age compared with a control non treated non stressed group (G4) at same periods. The results of antibody titer to NDV showed that there were significant (P < 0.05) differences among all groups at 7, 14 and 21 days of age, and the results of immunohistochemical analysis demonstrated positive staining for duodenal and bursal macrophages labeled with KUL-01 mouse anti-chicken monocyte- macrophages monoclonal antibodies. Tissue sections of duodenum and bursa at 14 and 24 days old stressed and non-stressed groups treated with β-glucan showed a positive result (purple-brown staining macrophages) in G1 and G3 as compared with duodenal and bursal tissues of G2 and G4groups which showed no stained cells.

scholarly journals Histopathological And Immune Response Against Infectious Bursal Disease In Chickens Vaccinated Against Newcastle Disease

2013 ◽  
Vol 12 (1) ◽  
pp. 66 ◽  
Author(s):  
A.Z. AL Zubeedy
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
High Antibody ◽  
Pathological Changes ◽  
Post Vaccination ◽  
Vaccination Programs ◽  
Bursal Disease ◽  
Antibody Titers

Two different vaccination programs against chicken infectious bursal disease (IBD) were compared. Results showed the first program including IBD vaccination at 8 and 16th day old as well as ND vaccination(live attenuated and inactivated types ) give high antibody titers at 21 days post vaccination with significantly difference (P≤0.0005) in comparison with the second program including only IBD vaccine . serological antibodies titer were determined to study the correlation between two different programs using ELISA test. pathological changes in vaccinated groups reveals mild & moderate lesions appear in liver, spleen and bursa of fabriciusKey word : IBD,ND , vaccination , ELISA test ,Broiler , histopathology .

scholarly journals Immune Response Study to Live Infectious Bursal Disease Vaccines in Broiler Chickens

2017 ◽  
Vol 6 (12) ◽  
pp. 2655-2668 ◽  
Author(s):  
Pooja Kundu ◽  
G. Narang ◽  
Rajesh Chhabra ◽  
Sushma Kajal ◽  
N.K. Mahajan ◽  
...  
Keyword(s):  
Immune Response ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Cellular immune response of infectious bursal disease and Newcastle disease vaccinations in broilers exposed to monochromatic lights

2011 ◽  
Vol 10 (46) ◽  
pp. 9528-9532 ◽  
Author(s):  
Sadrzadeh Avesta ◽  
Nikbakht Brujeni Gholamreza ◽  
Livi Masoud ◽  
Javad Nazari Mohammad ◽  
Tehrani Sharif Meysam ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Cellular Immune Response ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Cellular Immune

scholarly journals Comparative Immunogenicity Evaluation of Two Commonly Used Infectious Bursal Disease Vaccines in Broiler Chickens in Ethiopia

2021 ◽  
Author(s):  
Likelesh Legese ◽  
Berhane Wakjira ◽  
Tsedale Teshome ◽  
Dereje Nigussie Woldemichael ◽  
Hika Waktole ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Immunological Response ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
Differential Leukocyte Count ◽  
Endemic Diseases ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Group 2 ◽  
Group 1

Infectious bursal disease (IBD) is one of the most endemic diseases of commercial poultry in Ethiopia. Vaccination has been practiced as the major means of IBD prevention and control. A study was conducted to determine and compare the immunogenicity of two commercially available IBD vaccines in broiler chicken with maternally derived antibody (MDA). Day-old chickens of 270 were randomly assigned to three groups, group 1 vaccinated with brand 1 vaccine at 7th and 19th days and group 2 with brand 2 vaccine at 15th and 22nd days while group 3 were kept as control. Six chickens were also randomly selected and bled on day 1 for differential leukocyte count (DLC) and determination of MDA. Representative chickens from each group were bled at 24th and 42nd days of age for antibody titration using the indirect ELISA test. DLC scores were determined in the 1st and 24th days. The result revealed highly significant differences (P = 0.001) between group 1 and group 2 in DLC at 24th days of age. Antibody titers against IBD were differed significantly (P = 0.02) at 24th and 42nd days of age in broilers vaccinated with brand 1 and brand 2 vaccines. It is concluded that although both brands of vaccine induce an adequate immunological response at the end of the experiment, brand 1 vaccine has shown significantly high antibody titers against the IBDV and DLC than brand 2.

scholarly journals The Effect of Licorice roots powder on experimental Infection of infectious bursal disease in broiler chickens

2006 ◽  
Vol 30 (2) ◽  
pp. 65-78
Author(s):  
A. K. Kassab
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Food Intake ◽  
Water Content ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Age Group ◽  
Bursal Disease ◽  
Group 3 ◽  
Group 1

This study was conducted to investigate the effect of Licorice roots powder for 35 days in reducing the severity of infection with infectious bursal disease (IBD)and the immune response and performance of broiler chicks .A Total of 150 day old broiler chickens were divided into 5 groups and treated as follows : Group 1: infected with IBD virus at 21 days of age. Group 2: infected with IBD virus at 21 days of age and given 1 gm of Licorice roots powder /1 L of drinking water one day after infection . Group 3 : given 1 gm Licorice roots powder / 1L of drinking water at day one of age and infected with IBD virus at day 21 of age . Group 4:given 1 gm Licorice roots / 1 L of drinking water at 22 days of age .Group 5: untreated control. Antibody (Ab) of IBD titer was measured by ELIZA , the serum total protein (STP) the serum Globulin(SG)and the blood picture were determined , the water content of the droppings was measured , the body weight , food intake was recorded, there was increase (P<0.01) in the (Ab)titer of IBD virus in the group3 comparing with the other groups , the bursa / body weight index (B:BW)three days after infection was increased (P<0.01) in group1 , the STP , the SG , the PCV and RBCs was increased (P<0.01) in group 3 at age 27 days the food intake (FI) Food conversion ratio (FCR) and body weight (BW) in Group 3 were significantly increased , the dropping water content was significantly (P<0 .01)increased in group 3 in comparison with other groups at the period from 23-27 days of age, the morbidity and Mortality rate were significantly lower in group 3 and then 2 in comparison with group 1 (50 % , 63.3 %, 90 % ) and (6.6 % , 16.6 % ,30%) respectively

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